ML_AI_training/test_data/pnca_merged_df3.csv at 458a933d736b3cc75e65d437fe9a5b2adb6919e0

Tanushree Tunstall 04e0267dd1 added my_data4 after outputting merged_df3 for pnca to test the ml models

2022-03-03 13:35:05 +00:00

386 KiB

Raw Blame History

1	mutationinformation	lineage_labels	ligand_id	wild_type	wild_pos	position	mutant_type	pyrazinamide	drtype	mutation_info_labels	wt_prop_water	mut_prop_water	wt_prop_polarity	mut_prop_polarity	wt_calcprop	mut_calcprop	ligand_distance	ligand_affinity_change	duet_stability_change	ddg_foldx	deepddg	ddg_dynamut2	snap2_score	snap2_accuracy_pc	consurf_score	consurf_colour	consurf_colour_rev	asa	rsa	ss_class	kd_values	rd_values	af	or_mychisq	or_logistic	or_fisher	est_chisq	contacts	electro_rr	electro_sm	electro_ss	hbonds_rr	hbonds_mm	hbonds_sm	hbonds_ss	vdwclashes_rr	vdwclashes_mm	vdwclashes_sm	vdwclashes_ss	volumetric_rr	volumetric_sm	volumetric_ss	affinity_scaled	duet_scaled	foldx_scaled	deepddg_scaled	ddg_dynamut2_scaled	snap2_scaled	consurf_scaled	ligand_outcome	duet_outcome	foldx_outcome	deepddg_outcome	ddg_dynamut2_outcome	snap2_outcome	consurf_ci_upper	consurf_ci_lower	consurf_ci_colour	consurf_msa_data	consurf_aa_variety	beta_logistic	pval_logistic	se_logistic	zval_logistic	ci_low_logistic	ci_hi_logistic	log10_or_mychisq	pval_fisher	neglog_pval_fisher	ci_low_fisher	ci_hi_fisher	pval_chisq	lineage	mutation_info	mut_3upper	id	sample	sublineage	country_code	geographic_source	mutation	chain	ss	wt_3upper	wild_chain_pos	pdb_file
2	M1I	L4	PZA	M	M1	1	I	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	23.978	-0.294	-0.347	-0.0549615	-0.407	-0.79	7	53	-1.048	9	9	88	0.392857142857143	loop	0.633333333333333	4.12	0.0001437401178668	1.2119588062211	4.59700131334276e-05	0	1.01056822389159e-25	6	0	0	0	0	0	0	0	-3	0	-3	-3	0	0	0	-0.0744680851063829	-0.0896640826873385	-0.0105670806736907	-0.0693947144075021	-0.206806282722513	0.0736842105263158	-0.86326194398682	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-0.993	9:9	21/150	V:M	-9.98752126245056	0.942832958100734	139.277183325397	-0.0717096729269464	NA	144471.706314837	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage4	other_mutations_pyrazinamide	ILE	SAMN07236323,SRR5709866	SRR5709866	lineage4.4.2	th	Thailand	pnca_p.met1ile	A	-	MET	MA1	pnca_complex.pdb
3	M1K	L3	PZA	M	M1	1	K	0	MDR	OM	hydrophobic	hydrophobic	non-polar	basic	non-polar	pos	23.978	0.215	0.296	0.0794895	-1.017	-0.34	70	85	-1.048	9	9	88	0.392857142857143	loop	0.633333333333333	4.12	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-6	-2	0	-18	0	0	0	-2	-1	0	-1	-1	0	0	0	0.0964125560538116	0.251700680272109	0.00289518061757443	-0.173401534526854	-0.0890052356020942	0.736842105263158	-0.86326194398682	Stabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-0.993	9:9	21/150	V:M	-8.98740878149642	0.940032862253633	119.468044435492	-0.0752285585987748	NA	4692673.51081395	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	LYS	ERR2510542	ERR2510542	lineage3	pk	Pakistan	pnca_p.met1lys	A	-	MET	MA1	pnca_complex.pdb
4	M1R	L2	PZA	M	M1	1	R	NA	MDR	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	23.978	-0.16	0.694	-0.00977971	-0.78	1.21	44	71	-1.048	9	9	88	0.392857142857143	loop	0.633333333333333	4.12	NA	NA	NA	NA	NA	-14	0	0	-20	-2	0	-2	0	1	0	-1	1	0	-2	-2	-0.0405268490374873	0.590136054421769	-0.00188027955087288	-0.132992327365729	0.679775280898876	0.463157894736842	-0.86326194398682	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-1.178	-0.993	9:9	21/150	V:M	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	ARG	SRR5073787	SRR5073787	lineage2.2.1	vn	Vietnam	pnca_p.met1arg	A	-	MET	MA1	pnca_complex.pdb
5	M1T	L4	PZA	M	M1	1	T	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	23.978	0.344	0.109	0.867771	-1.096	0.51	52	75	-1.048	9	9	88	0.392857142857143	loop	0.633333333333333	4.12	0.0007187005893344	97.3828619670747	1395803.74890195	Inf	42.8452490786805	10	0	0	0	0	0	0	0	2	0	0	2	0	0	0	0.154260089686099	0.0926870748299319	0.031606108727482	-0.186871270247229	0.286516853932584	0.547368421052632	-0.86326194398682	Stabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.178	-0.993	9:9	21/150	V:M	14.1489809714062	0.890414731995374	102.692976014008	0.137779442378573	507.712885368923	NA	1.98848253379833	2.1018584548314e-08	7.67739653395739	10.9022485555592	Inf	5.92457926729285e-11	lineage4	dr_mutations_pyrazinamide	THR	ERR2515926	ERR2515926	lineage4.8	za	South Africa	pnca_p.met1thr	A	-	MET	MA1	pnca_complex.pdb
6	R2L	L4	PZA	R	R2	2	L	0	Pre-XDR	OM	hydrophilic	hydrophobic	basic	non-polar	pos	non-polar	21.092	-2.083	0.062	1.78755	-0.708	-0.24	44	71	-0.015	5	5	55	0.200729927007299	strand	-0.266666666666667	4.74	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	38	30	4	26	4	0	2	2	1	0	-2	2	0	0	-2	-0.527608915906788	0.0527210884353741	0.0651064620225963	-0.120716112531969	-0.06282722513089	0.463157894736842	-0.0123558484349259	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	84/150	R:K:N:H:T:S:E	-8.9874087814964	0.940032862253633	119.46804443549	-0.0752285585987755	NA	4692673.51081259	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	LEU	ERR2516706	ERR2516706	lineage4	pe	Peru	pnca_p.arg2leu	A	E	ARG	RA2	pnca_complex.pdb
7	R2P	L4	PZA	R	R2	2	P	NA	MDR	OM	hydrophilic	hydrophilic	basic	non-polar	pos	non-polar	21.092	-2.244	-1.031	4.20376	-1.935	-0.43	63	80	-0.015	5	5	55	0.200729927007299	strand	-0.266666666666667	4.74	NA	NA	NA	NA	NA	48	28	4	24	2	0	4	0	4	2	1	1	4	6	0	-0.56838905775076	-0.26640826873385	0.153110089671399	-0.329923273657289	-0.112565445026178	0.663157894736842	-0.0123558484349259	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	84/150	R:K:N:H:T:S:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	PRO	ERR779907	ERR779907	lineage4.3.3	pe		pnca_p.arg2pro	A	E	ARG	RA2	pnca_complex.pdb
8	R2W	L4	PZA	R	R2	2	W	NA	Pre-XDR	OM	hydrophilic	hydrophobic	basic	non-polar	pos	non-polar	21.092	-1.147	-0.164	4.38565	-0.74	-0.16	58	75	-0.015	5	5	55	0.200729927007299	strand	-0.266666666666667	4.74	NA	NA	NA	NA	NA	8	30	4	26	4	0	2	2	0	0	-5	5	6	6	4	-0.290526849037487	-0.0423772609819121	0.159734919397723	-0.12617220801364	-0.0418848167539267	0.610526315789474	-0.0123558484349259	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	84/150	R:K:N:H:T:S:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	TRP	SAMN02584674	SRR1168960_SRR1166135	lineage4.2.1	md	Moldova	pnca_p.arg2trp	A	E	ARG	RA2	pnca_complex.pdb
9	A3E	L2	PZA	A	A3	3	E	1	MDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	18.028	-1.552	-2.829	2.75936	-5.34	-1.11	56	75	-0.873	8	8	0	0	strand	0.366666666666667	8.38	0.0002874802357337	38.8547368421053	1392277.50785087	Inf	13.9909822352959	-30	-38	-2	-36	0	0	0	0	-13	0	-3	-14	-4	-2	-9	-0.393110435663627	-0.731007751937984	0.100501897595408	-0.910485933503836	-0.290575916230366	0.589473684210526	-0.719110378912685	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	141/150	A:G:T:V:C	14.1464514590954	0.930573112990226	162.371849599086	0.0871237932808215	0.011399544335555	NA	1.58944397188817	0.0008528275207519	3.06913879340442	3.20302487654751	Inf	0.0001836895199759	lineage2	dr_mutations_pyrazinamide	GLU	MDR_17174	SRR6824459	lineage2.2.1	cn	China: Ma City, Huanggang City,Hubei Province	pnca_p.ala3glu	A	E	ALA	AA3	pnca_complex.pdb
10	A3P	L4	PZA	A	A3	3	P	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	18.028	-2.664	-1.124	-0.155697	-3.837	-0.13	66	80	-0.873	8	8	0	0	strand	0.366666666666667	8.38	7.18700589334483e-05	9.70142977291842	188179.926264599	Inf	0.763784339928386	0	0	0	0	0	0	0	0	-7	2	-7	-4	-2	-2	-1	-0.674772036474164	-0.290439276485788	-0.0299348227332154	-0.654219948849105	-0.0340314136125654	0.694736842105263	-0.719110378912685	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	141/150	A:G:T:V:C	12.1451538387263	0.919026337424228	119.468044436222	0.101660271548263	5.01153666063644e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	PRO	3200	ERR2432999	lineage4.1.2.1	br	Brazil	pnca_p.ala3pro	A	E	ALA	AA3	pnca_complex.pdb
11	L4S	L2	PZA	L	L4	4	S	1	MDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	15.328	-1.078	-3.87	5.57838	-2.638	-3.7	63	80	-1.075	9	9	0	0	strand	3.36666666666667	8.8	0.0024435820037372	28.4570212765957	28.4570177059942	28.4308865607855	107.057952768928	22	0	0	0	0	0	-2	0	-3	0	-1	-2	6	4	6	-0.273049645390071	-1	0.203176742254824	-0.449786871270247	-0.968586387434555	0.663157894736842	-0.885502471169687	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.152	-1.028	9:9	146/150	F:V:L:I	3.34839479820379	4.89069202996555e-12	0.484662577645081	6.90871330415739	12.0071584873314	83.7151686983107	1.4541894385859	5.6263316435115e-18	17.2497746719749	10.8739157094292	94.2522723934353	4.32340113554558e-25	lineage2	dr_mutations_pyrazinamide	SER	ERR2516880	ERR2516880	lineage2.2.1	gb	UK	pnca_p.leu4ser	A	E	LEU	LA4	pnca_complex.pdb
12	L4V	L6	PZA	L	L4	4	V	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.328	-2.828	-1.717	1.19624	-1.68	-1.95	22	63	-1.075	9	9	0	0	strand	3.36666666666667	8.8	NA	NA	NA	NA	NA	22	0	0	0	0	0	0	0	-3	0	-1	-2	2	0	0	-0.716312056737589	-0.443669250645995	0.043569664697441	-0.286445012787724	-0.510471204188482	0.231578947368421	-0.885502471169687	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.152	-1.028	9:9	146/150	F:V:L:I	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage6	other_mutations_pyrazinamide	VAL	ERR1215461	ERR1215461	lineage6	gh	Ghana	pnca_p.leu4val	A	E	LEU	LA4	pnca_complex.pdb
13	L4W	L2	PZA	L	L4	4	W	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.328	-2.216	-2.169	1.63111	-2.168	-1.93	70	85	-1.075	9	9	0	0	strand	3.36666666666667	8.8	0.0012217910018686	22.7551797040169	22.7551786098964	22.7463684762183	46.6274188614457	-46	6	0	6	0	0	0	0	-15	0	-7	-8	0	-16	2	-0.5612968591692	-0.56046511627907	0.0594085766941776	-0.369650468883205	-0.505235602094241	0.736842105263158	-0.885502471169687	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.152	-1.028	9:9	146/150	F:V:L:I	3.12479275069714	9.15103299018708e-07	0.636534850451638	4.90906782005734	7.42096527532349	98.7712876576624	1.35708026960092	7.15795820641824e-09	8.14521084167875	6.3414443600848	123.179124931302	8.58505795592483e-12	lineage2	dr_mutations_pyrazinamide	TRP	ERR3275831	ERR3275831	lineage2.2.1	nl	Netherlands	pnca_p.leu4trp	A	E	LEU	LA4	pnca_complex.pdb
14	I5S	L4	PZA	I	I5	5	S	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	11.437	-0.778	-3.843	5.83176	-3.518	-3.58	63	80	-0.011	5	5	0	0	strand	4.26666666666667	12.6	7.18700589334483e-05	9.70142977291842	188179.92626441	Inf	0.763784339928386	30	0	0	0	0	0	-4	0	4	0	2	2	0	4	0	-0.197061803444782	-0.993023255813954	0.212405393395931	-0.599829497016198	-0.93717277486911	0.663157894736842	-0.00906095551894563	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	146/150	G:F:I:L:V	12.1451538387253	0.919026337424193	119.46804443616	0.101660271548307	5.0115366606943e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	SER	ERR2179669	ERR2179669	lineage4.4	cn	China	pnca_p.ile5ser	A	E	ILE	IA5	pnca_complex.pdb
15	I5T	L1	PZA	I	I5	5	T	1	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	11.437	-0.945	-3.43	3.67776	-2.709	-3.3	63	80	-0.011	5	5	0	0	strand	4.26666666666667	12.6	0.0002874802357337	14.5631313131313	14.5630810302278	14.5631012253236	5.81873994206135	32	0	0	0	0	0	-4	0	4	0	2	2	0	4	0	-0.23936170212766	-0.886304909560723	0.133952024708805	-0.461892583120205	-0.863874345549738	0.663157894736842	-0.00906095551894563	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	146/150	G:F:I:L:V	2.67848962960141	0.0202679102821362	1.15384949648317	2.3213509541454	1.86363317860697	294.488177946116	1.16325476548539	0.0174140488202037	1.75910024233227	1.16837785201846	760.989759542733	0.0158563003544608	lineage1	dr_mutations_pyrazinamide	THR	ERR2510512	ERR2510512	lineage1.2.2	pk	Pakistan	pnca_p.ile5thr	A	E	ILE	IA5	pnca_complex.pdb
16	I6L	L2	PZA	I	I6	6	L	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.258	-3.018	-0.548	0.267883	-1.616	-0.58	5	53	-0.6	7	7	1	0.0050761421319796	strand	4.4	11.36	0.0097024579560155	0.385310257492613	0.385311080310026	0.385326683377145	8.35414540919546	-6	0	0	0	0	0	0	0	-7	0	-2	-5	2	-2	-2	-0.764437689969605	-0.141602067183462	0.00975688197029407	-0.275532821824382	-0.151832460732984	0.0526315789473684	-0.494233937397035	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.553	8:7	146/150	M:A:I:L:V	-0.953704270146009	0.0037560422096937	0.329095586242593	-2.89795521427317	0.189089468462042	0.697385389891155	-0.414189429312607	0.0017355697972772	2.76055791615401	0.180049643960023	0.733980750878148	0.0038480840706912	lineage2	other_mutations_pyrazinamide	LEU	ERR067611,0341X-sc-2011-09-07T13:19:07Z-1276458	ERR067611	lineage2.2.1	ru	Russia	pnca_p.ile6leu	A	E	ILE	IA6	pnca_complex.pdb
17	I6M	L4	PZA	I	I6	6	M	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.258	-3.085	-1.021	0.24349	-2.364	0.14	10	59	-0.6	7	7	1	0.0050761421319796	strand	4.4	11.36	NA	NA	NA	NA	NA	-32	0	0	0	0	0	0	0	-3	0	0	-3	-2	-4	-2	-0.781408308004053	-0.263824289405685	0.00886843581319794	-0.40306905370844	0.0786516853932584	0.105263157894737	-0.494233937397035	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.728	-0.553	8:7	146/150	M:A:I:L:V	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	MET	ERR1555057	ERR1555057	lineage4.3.3			pnca_p.ile6met	A	E	ILE	IA6	pnca_complex.pdb
18	I6S	L2	PZA	I	I6	6	S	1	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	9.258	-1.047	-3.585	6.01751	-4.149	-3.42	64	80	-0.6	7	7	1	0.0050761421319796	strand	4.4	11.36	0.0001437401178668	19.411022297013	511754.463552573	Inf	4.73122070382274	24	0	0	0	0	0	-2	0	-1	0	-1	0	2	4	0	-0.265197568389058	-0.926356589147287	0.219170812724452	-0.707416879795396	-0.895287958115183	0.673684210526316	-0.494233937397035	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.553	8:7	146/150	M:A:I:L:V	13.1456002256205	0.924803768446996	139.277183326562	0.094384449136928	0.0001628371344453	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	SER	ERR2516977	ERR2516977	lineage2.2.1	gb	UK	pnca_p.ile6ser	A	E	ILE	IA6	pnca_complex.pdb
19	I6T	L2	PZA	I	I6	6	T	1	XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	9.258	-1.244	-3.089	3.71452	-3.589	-3.14	62	80	-0.6	7	7	1	0.0050761421319796	strand	4.4	11.36	0.000646830530401	87.6075949367089	1395214.80217275	Inf	38.0099632572142	24	0	0	0	0	0	-2	0	-1	0	-1	0	2	0	0	-0.315096251266464	-0.798191214470284	0.135290903925582	-0.611935208866155	-0.821989528795812	0.652631578947368	-0.494233937397035	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.553	8:7	146/150	M:A:I:L:V	14.148558941436	0.896008552350165	108.247901039825	0.130705157379733	226.474535586036	NA	1.94254175795031	1.23317897951184e-07	6.90897388676731	9.59561062779463	Inf	7.038428913028e-10	lineage2	dr_mutations_pyrazinamide	THR	ERR2510686	ERR2510686	lineage2.2.1	it	Italy	pnca_p.ile6thr	A	E	ILE	IA6	pnca_complex.pdb
20	I6V	L1	PZA	I	I6	6	V	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.258	-2.761	-1.528	1.33147	-1.511	-1.35	-86	93	-0.6	7	7	1	0.0050761421319796	strand	4.4	11.36	NA	NA	NA	NA	NA	24	0	0	0	0	0	0	0	-1	0	-1	0	2	2	0	-0.699341438703141	-0.394832041343669	0.048495035657311	-0.257630008525149	-0.353403141361257	-0.924731182795699	-0.494233937397035	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.728	-0.553	8:7	146/150	M:A:I:L:V	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	VAL	SAMN02599161	SRR1173036_SRR1172709	lineage1.1.3	in	India	pnca_p.ile6val	A	E	ILE	IA6	pnca_complex.pdb
21	V7A	L4	PZA	V	V7	7	A	NA	Other	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.336	-0.97	-2.592	3.42668	-3.043	-1.9	64	80	-0.66	7	7	0	0	strand	1.73333333333333	11.02	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	2	0	0	0	0	0	-2	0	5	0	3	2	4	6	3	-0.245694022289767	-0.669767441860465	0.124807144574188	-0.518840579710145	-0.49738219895288	0.673684210526316	-0.543657331136738	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	146/150	V:I:A	-8.98740878149641	0.940032862253633	119.468044435491	-0.075228558598775	NA	4692673.51081185	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	ALA	ERR2229336	ERR2229336	lineage4.8	dk		pnca_p.val7ala	A	E	VAL	VA7	pnca_complex.pdb
22	V7F	L2	PZA	V	V7	7	F	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.336	0.042	-1.877	4.86315	-2.949	-1.55	83	91	-0.66	7	7	0	0	strand	1.73333333333333	11.02	0.0002156101768003	9.70466975178797	9.70465898653049	9.70194990589383	2.29168245016684	-54	-2	0	-2	0	0	-2	0	-3	0	1	-4	-8	-6	-4	0.0188340807174887	-0.485012919896641	0.177126508788671	-0.502813299232737	-0.405759162303665	0.873684210526316	-0.543657331136738	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	146/150	V:I:A	2.27260607808817	0.06344559022609	1.22442760158496	1.8560559033024	0.929172866365903	208.881603004777	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	dr_mutations_pyrazinamide	PHE	ERR2510561	ERR2510561	lineage2.2.1	pk	Pakistan	pnca_p.val7phe	A	E	VAL	VA7	pnca_complex.pdb
23	V7G	L2	PZA	V	V7	7	G	NA	XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	5.336	-1.062	-3.114	5.26226	-4.33	-2.58	86	91	-0.66	7	7	0	0	strand	1.73333333333333	11.02	0.001293661060802	39.0452814219213	39.0452813634446	39.0430804499264	60.5607562088978	12	0	0	0	0	0	-2	0	3	0	1	2	8	11	21	-0.268996960486322	-0.804651162790698	0.191662963745365	-0.738277919863598	-0.675392670157068	0.905263157894737	-0.543657331136738	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	146/150	V:I:A	3.6647220332212	1.03849290922087e-06	0.750321763717271	4.88420063289289	11.103639297077	247.090478887866	1.59156855738766	5.52264644232101e-11	10.2578527592503	9.16748833639401	349.97982252054	7.13421964779937e-15	lineage2	dr_mutations_pyrazinamide	GLY	SAMN02628499	SRR1172711_SRR1173722	lineage2.2.1	ir	Iran	pnca_p.val7gly	A	E	VAL	VA7	pnca_complex.pdb
24	V7I	L4	PZA	V	V7	7	I	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.336	-0.635	-0.898	-1.10338	-0.738	-0.73	46	71	-0.66	7	7	0	0	strand	1.73333333333333	11.02	NA	NA	NA	NA	NA	-24	0	0	0	0	0	-2	0	-4	0	2	-7	0	2	-2	-0.160840932117528	-0.232041343669251	-0.212139506267784	-0.125831202046036	-0.19109947643979	0.484210526315789	-0.543657331136738	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	146/150	V:I:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	ILE	SAMN02586003	SRR1196479_SRR1175056_SRR1184351_SRR1172851	lineage4.3.4.2	za	South Africa	pnca_p.val7ile	A	E	VAL	VA7	pnca_complex.pdb
25	V7L	L4	PZA	V	V7	7	L	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.336	-0.589	-1.137	-0.847275	-2.289	-0.99	83	91	-0.66	7	7	0	0	strand	1.73333333333333	11.02	0.0009343107661348	10.9471518987342	10.9471289278841	10.9440461901209	21.4040928993518	-26	0	0	0	0	0	-2	0	-3	0	1	-4	-2	-2	2	-0.14918946301925	-0.293798449612403	-0.162899907713605	-0.390281329923274	-0.259162303664921	0.873684210526316	-0.543657331136738	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	146/150	V:I:A	2.39307722351576	6.83262964849249e-05	0.600967660910521	3.98203993188257	3.56185012264106	40.4315613280321	1.03930114423982	4.53835196843674e-05	4.34310182576657	3.05117591386949	48.6969925271007	3.71976276752962e-06	lineage4	dr_mutations_pyrazinamide	LEU	140603921,SAMN05276312	SRR3675218	lineage4.1.1.3	gb	UK	pnca_p.val7leu	A	E	VAL	VA7	pnca_complex.pdb
26	D8A	L2	PZA	D	D8	8	A	NA	Pre-XDR	DM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	3.22	-3.268	-0.511	0.541028	-1.125	0.4	87	91	-1.187	9	9	5	0.0259067357512953	loop	1.63333333333333	9.48	0.0001437401178668	19.411022297013	511754.463552623	Inf	4.73122070382274	26	36	0	36	0	0	0	4	-5	0	-4	-1	0	6	1	-0.827760891590679	-0.132041343669251	0.0197054174345676	-0.191815856777494	0.224719101123595	0.91578947368421	-0.977759472817133	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.178	9:9	146/150	D:H	13.1456002256206	0.924803768447001	139.277183326572	0.094384449136922	0.0001628371344451	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	ALA	SRR5709862,SAMN07236319	SRR5709862	lineage2.2.1	th	Thailand	pnca_p.asp8ala	A	-	ASP	DA8	pnca_complex.pdb
27	D8E	L4	PZA	D	D8	8	E	NA	Pre-XDR	DM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	3.22	0.012	-0.786	1.90122	-1.417	-0.4	90	95	-1.187	9	9	5	0.0259067357512953	loop	1.63333333333333	9.48	0.0002874802357337	14.5631313131313	14.5630810302278	14.5631012253236	5.81873994206135	-24	-28	0	-28	-2	0	-2	0	2	0	2	-1	-8	-10	-6	0.0053811659192825	-0.203100775193798	0.0692465708520604	-0.241602728047741	-0.104712041884817	0.947368421052632	-0.977759472817133	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	146/150	D:H	2.67848962960141	0.0202679102821361	1.15384949648317	2.3213509541454	1.86363317860697	294.488177945815	1.16325476548539	0.0174140488202037	1.75910024233227	1.16837785201846	760.989759542733	0.0158563003544608	lineage4	dr_mutations_pyrazinamide	GLU	ERR2510171	ERR2510171	lineage4	pe	Peru	pnca_p.asp8glu	A	-	ASP	DA8	pnca_complex.pdb
28	D8G	L2;L4	PZA	D	D8	8	G	NA	MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	3.22	-3.445	-0.851	1.89305	-1.078	-0.82	92	95	-1.187	9	9	5	0.0259067357512953	loop	1.63333333333333	9.48	0.0007905706482679	48.6872097931617	48.6872048041474	48.6746878162623	37.2619168572472	34	38	0	38	0	0	0	0	-8	-1	-3	-5	6	9	17	-0.872593718338399	-0.219896640826873	0.0689490016681357	-0.183802216538789	-0.214659685863874	0.968421052631579	-0.977759472817133	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	146/150	D:H	3.88541626053988	0.0002118075877291	1.0488367141091	3.70450062271154	9.3197738725557	893.78337229237	1.68741488635211	1.95392414212297e-07	6.70909230105579	6.91781679568266	2088.84994220519	1.03281930722422e-09	lineage2;lineage4	dr_mutations_pyrazinamide	GLY	SRR5818641	SRR5818641	lineage4;lineage2.2.1	dj	Djibouti	pnca_p.asp8gly	A	-	ASP	DA8	pnca_complex.pdb
29	D8N	L2	PZA	D	D8	8	N	NA	MDR	DM	hydrophilic	hydrophilic	acidic	neutral	neg	polar	3.22	-1.655	-1.182	-1.26057	-1.022	-1.29	92	95	-1.187	9	9	5	0.0259067357512953	loop	1.63333333333333	9.48	0.0005030904125341	29.1630847029077	29.1630732435187	29.1293325978315	18.6717493808583	0	34	0	34	-4	0	-2	-2	-7	0	-5	-4	0	-4	2	-0.41919959473151	-0.305426356589147	-0.242361378143505	-0.174254049445865	-0.337696335078534	0.968421052631579	-0.977759472817133	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	146/150	D:H	3.37290329402598	0.001789077082818	1.07995768762031	3.12318096596746	4.97876379054103	550.894323519252	1.46483345924118	0.0001485295621003	3.828187099348	3.53443753556636	1331.56873199938	1.55265977742841e-05	lineage2	dr_mutations_pyrazinamide	ASN	ERR2515173	ERR2515173	lineage2.2.1	za	South Africa	pnca_p.asp8asn	A	-	ASP	DA8	pnca_complex.pdb
30	V9A	L2	PZA	V	V9	9	A	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	6.555	-1.286	-2.467	2.03689	-2.068	-2.01	61	80	-0.711	8	8	0	0	loop	-0.933333333333333	9.57	0.0002156101768003	0.807902480033628	4.59660271789382e-05	0	0.000393645565148	0	0	0	0	0	0	0	0	2	0	0	2	6	6	5	-0.325734549138804	-0.637467700258398	0.0741879675696938	-0.352600170502984	-0.526178010471204	0.642105263157895	-0.585667215815486	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.827	-0.615	8:7	146/150	I:C:L:V:M	-9.98760797391819	0.930014323968985	113.719344729607	-0.0878268160765962	NA	127.948735098955	-0.0926410587047397	1	0	0	11.7369754738005	0.984170607395186	lineage2	dr_mutations_pyrazinamide	ALA	SRS797047	SRR1723549	lineage2.2.1	cn	China:Hebei	pnca_p.val9ala	A	-	VAL	VA9	pnca_complex.pdb
31	V9G	L3	PZA	V	V9	9	G	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	6.555	-1.063	-3.236	3.70952	-3.636	-3.48	83	91	-0.711	8	8	0	0	loop	-0.933333333333333	9.57	0.0003593502946672	19.4256842105263	19.4256680571767	19.4169790983636	9.87561363723508	8	0	0	0	0	0	0	0	0	0	2	-2	4	3	21	-0.269250253292806	-0.836175710594315	0.135108793041907	-0.619948849104859	-0.910994764397906	0.873684210526316	-0.585667215815486	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.827	-0.615	8:7	146/150	I:C:L:V:M	2.96659528728018	0.0079500503166053	1.11770528264822	2.65418382943607	2.87215213651501	380.097158975938	1.28837632444196	0.0036816889015929	2.43395291165754	1.92061868691892	951.403114304703	0.0016748373055023	lineage3	dr_mutations_pyrazinamide	GLY	ERR2510586	ERR2510586	lineage3	pk	Pakistan	pnca_p.val9gly	A	-	VAL	VA9	pnca_complex.pdb
32	Q10E	L2	PZA	Q	Q10	10	E	NA	XDR	OM	hydrophilic	hydrophilic	neutral	acidic	polar	neg	6.02	-0.537	-2.051	3.84692	-1.976	-0.13	88	91	-1.214	9	9	0	0	strand	-0.933333333333333	8.14	NA	NA	NA	NA	NA	0	-56	0	-56	0	0	2	-2	-4	0	-6	1	0	0	4	-0.136018237082067	-0.529974160206718	0.140113200125292	-0.33691389599318	-0.0340314136125654	0.926315789473684	-1	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLU	DRR184878	DRR184878	lineage2.2.1	vn	Vietnam	pnca_p.gln10glu	A	B	GLN	QA10	pnca_complex.pdb
33	Q10H	L4	PZA	Q	Q10	10	H	1	MDR	DM	hydrophilic	hydrophobic	neutral	basic	polar	pos	6.02	-0.636	-1.024	3.94633	-1.814	-0.97	91	95	-1.214	9	9	0	0	strand	-0.933333333333333	8.14	0.0007905706482679	107.166385135135	3795803.48336072	Inf	47.6847963834956	4	0	0	0	2	0	2	0	-11	0	-8	-3	0	2	2	-0.161094224924012	-0.264599483204134	0.143733928714516	-0.309292412617221	-0.253926701570681	0.957894736842105	-1	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	Q	15.1494066679727	0.925233417916174	161.432542878774	0.0938435732834176	24.1993753469043	NA	2.03005858162844	3.58120158191569e-09	8.44597123233921	12.2081619622864	Inf	5.00558510128865e-12	lineage4	dr_mutations_pyrazinamide	HIS	436	ERR3014581	lineage4.1.2.1	tn	Tunisia:Bizerte	pnca_p.gln10his	A	B	GLN	QA10	pnca_complex.pdb
34	Q10L	L4	PZA	Q	Q10	10	L	NA	Pre-XDR	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	6.02	-1.694	0.641	0.169275	-0.188	0.05	91	95	-1.214	9	9	0	0	strand	-0.933333333333333	8.14	NA	NA	NA	NA	NA	20	0	0	0	4	0	4	0	-3	0	-3	-3	2	6	4	-0.429078014184397	0.545068027210884	0.00616536396681211	-0.0320545609548167	0.0280898876404494	0.957894736842105	-1	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.229	-1.201	9:9	147/150	Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	LEU	SAMN02567710	SRR1166324	lineage4.1.2.1	ro	Romania	pnca_p.gln10leu	A	B	GLN	QA10	pnca_complex.pdb
35	Q10P	L4	PZA	Q	Q10	10	P	1	Pre-XDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	6.02	-1.771	-0.631	6.78299	-2.963	-0.11	95	95	-1.214	9	9	0	0	strand	-0.933333333333333	8.14	0.0211297973264338	156.233890214797	156.233722233267	156.382472756358	1333.51093453962	38	-2	0	-2	6	2	4	0	-8	2	-6	-4	0	2	0	-0.448581560283688	-0.163049095607235	0.247051260571537	-0.505200341005968	-0.0287958115183246	1	-1	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	Q	5.05135310548255	2.49562215648785e-55	0.322395320006857	15.6681961306855	87.7376148601903	314.979076956589	2.19377524680641	1.27822918710216e-207	206.893391269988	83.6783303393677	330.782028899935	5.90060424845557e-292	lineage4	dr_mutations_pyrazinamide	PRO	ERR760750	ERR760750	lineage4.1.2.1	ar	Argentina	pnca_p.gln10pro	A	B	GLN	QA10	pnca_complex.pdb
36	Q10R	L4	PZA	Q	Q10	10	R	NA	MDR	DM	hydrophilic	hydrophilic	neutral	basic	polar	pos	6.02	-0.833	-1.078	8.55341	-2.07	-0.42	94	95	-1.214	9	9	0	0	strand	-0.933333333333333	8.14	0.001293661060802	83.0135478408129	83.0096797665738	82.9817212776157	70.7034697627924	-48	-32	0	-32	2	0	2	0	-5	0	-5	-5	0	6	-4	-0.210992907801418	-0.278552971576227	0.311533810706663	-0.352941176470588	-0.109947643979058	0.989473684210526	-1	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	Q	4.41895722469024	1.61039699449321e-05	1.02456224249978	4.31301978678098	17.0476857395373	1496.24228456829	1.91914897517566	1.31421065040623e-12	11.8813350175945	12.9924324330291	3402.15298704802	4.15161653132659e-17	lineage4	dr_mutations_pyrazinamide	ARG	SRR3732721	SRR3732721	lineage4.3.3	pe	Peru	pnca_p.gln10arg	A	B	GLN	QA10	pnca_complex.pdb
37	N11S	L3	PZA	N	N11	11	S	0	Sensitive	OM	hydrophilic	hydrophilic	neutral	neutral	polar	polar	9.599	-1.515	0.315	2.58536	-1.389	0.57	69	80	-0.682	8	8	42	0.215384615384615	helix	-3.5	4.28	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	4	0	0	0	2	0	2	0	-2	0	0	-2	0	-2	0	-0.383738601823708	0.267857142857143	0.094164438843523	-0.236828644501279	0.320224719101123	0.726315789473684	-0.561779242174629	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.827	-0.615	8:7	147/150	T:P:L:N:V:I:R:K:Y:E:H:Q	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987755	NA	4692673.51081015	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	SER	ERR2513021	ERR2513021	lineage3	gb	UK	pnca_p.asn11ser	A	G	ASN	NA11	pnca_complex.pdb
38	D12A	L4	PZA	D	D12	12	A	NA	MDR	DM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	7.825	-2.924	0.904	0.896575	-1.267	1.36	64	80	-1.058	9	9	22	0.113989637305699	helix	-1.4	5.01	0.0017967514733362	5.2760989010989	5.27609886875925	5.2752786493537	19.1276037177328	14	16	0	16	4	0	4	0	0	0	0	0	4	6	3	-0.740628166160081	0.768707482993197	0.0326552131061561	-0.216027280477408	0.764044943820225	0.673684210526316	-0.871499176276771	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.124	-1.028	9:9	147/150	C:D:T:G	1.66318697401976	3.35314758209503e-05	0.400956055116994	4.14805301676878	2.39021356042842	11.7435962131774	0.722312927852139	6.93631068428177e-05	4.15887146261268	2.21601547098056	12.6651520546836	1.22263744860911e-05	lineage4	dr_mutations_pyrazinamide	ALA	ERR2707093	ERR2707093	lineage4.3.2	cd	Democratic Republic of Congo	pnca_p.asp12ala	A	G	ASP	DA12	pnca_complex.pdb
39	D12E	L2	PZA	D	D12	12	E	NA	MDR	DM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	7.825	-0.42	-0.21	2.1586	-2.035	-0.02	69	80	-1.058	9	9	22	0.113989637305699	helix	-1.4	5.01	0.0005749604714675	34.0379426644182	34.0375872334386	34.0436365315203	23.2409378193551	-8	0	0	0	2	0	2	0	-3	0	-3	-2	0	0	0	-0.106382978723404	-0.0542635658914728	0.0786209107001071	-0.346973572037511	-0.0052356020942408	0.726315789473684	-0.871499176276771	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-1.028	9:9	147/150	C:D:T:G	3.52746542085928	0.0009482725856347	1.06716956280854	3.30544043214259	6.05331233477717	636.464952477748	1.53196330239691	2.88610345466244e-05	4.53968810533409	4.36820226973726	1521.29182901896	1.42922316872435e-06	lineage2	dr_mutations_pyrazinamide	GLU	ERR234215	ERR234215	lineage2.2.1	mn	Mongolia	pnca_p.asp12glu	A	G	ASP	DA12	pnca_complex.pdb
40	D12G	L4	PZA	D	D12	12	G	1	Pre-XDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	7.825	-3.008	0.079	2.26095	-1.546	0.46	67	80	-1.058	9	9	22	0.113989637305699	helix	-1.4	5.01	0.0005030904125341	29.1630847029077	29.1630732435187	29.1293325978315	18.6717493808583	20	16	0	16	4	0	4	0	0	0	0	0	6	15	17	-0.761904761904762	0.0671768707482993	0.0823487204889313	-0.263597612958227	0.258426966292135	0.705263157894737	-0.871499176276771	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.124	-1.028	9:9	147/150	C:D:T:G	3.37290329402598	0.001789077082818	1.07995768762031	3.12318096596747	4.97876379053956	550.894323519286	1.46483345924118	0.0001485295621003	3.828187099348	3.53443753556636	1331.56873199938	1.55265977742841e-05	lineage4	dr_mutations_pyrazinamide	GLY	ERR2516704	ERR2516704	lineage4.3.2	pe	Peru	pnca_p.asp12gly	A	G	ASP	DA12	pnca_complex.pdb
41	D12N	L2	PZA	D	D12	12	N	NA	Pre-XDR	DM	hydrophilic	hydrophilic	acidic	neutral	neg	polar	7.825	-1.928	0.018	1.58277	-2.236	0.09	79	85	-1.058	9	9	22	0.113989637305699	helix	-1.4	5.01	0.000646830530401	17.0174957841484	17.0174574529133	17.0076332291246	19.306440114962	0	16	0	16	2	0	2	0	-7	0	-5	-2	0	0	0	-0.488348530901722	0.0153061224489795	0.0576479286708091	-0.381244671781756	0.0505617977528089	0.831578947368421	-0.871499176276771	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.124	-1.028	9:9	147/150	C:D:T:G	2.83423972614077	0.0004057565735776	0.801467871651648	3.53631109416779	4.11022012991606	114.25813127402	1.23089565168614	0.0001105447439914	3.95646190174896	3.23648804294829	167.757055506696	1.11330211002346e-05	lineage2	dr_mutations_pyrazinamide	ASN	SRS797316	SRR1723847	lineage2.2.1	cn	China:Shanxi	pnca_p.asp12asn	A	G	ASP	DA12	pnca_complex.pdb
42	D12Y	L4	PZA	D	D12	12	Y	NA	MDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	7.825	-1.94	-0.794	4.93098	-1.092	-0.59	82	91	-1.058	9	9	22	0.113989637305699	helix	-1.4	5.01	NA	NA	NA	NA	NA	-30	16	0	16	4	0	4	0	-7	0	-7	-2	0	0	-6	-0.491388044579534	-0.205167958656331	0.179597025036604	-0.18618925831202	-0.154450261780105	0.863157894736842	-0.871499176276771	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-1.028	9:9	147/150	C:D:T:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	TYR	ERR047881	ERR047881	lineage4.3.3	gb	UK	pnca_p.asp12tyr	A	G	ASP	DA12	pnca_complex.pdb
43	F13C	L4	PZA	F	F13	13	C	NA	Pre-MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	3.551	-0.485	-2.32	2.69734	-2.036	-2.68	79	85	-1.201	9	9	24	0.1	helix	0.6	6.93	0.0001437401178668	1.2119588062211	4.59700131334268e-05	0	1.01056822389159e-25	42	4	0	4	0	0	-2	0	-7	0	-5	-2	-2	-2	0	-0.122847011144883	-0.599483204134367	0.098242994194305	-0.347144075021313	-0.701570680628272	0.831578947368421	-0.989291598023064	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	F	-9.98752126245058	0.942832958100734	139.277183325397	-0.0717096729269463	NA	144471.706314842	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage4	other_mutations_pyrazinamide	CYS	ERR1034975	ERR1034975	lineage4.3.2.1	za	South Africa	pnca_p.phe13cys	A	G	PHE	FA13	pnca_complex.pdb
44	F13I	L4	PZA	F	F13	13	I	1	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	3.551	-0.45	-1.762	0.887245	-1.241	-2.3	83	91	-1.201	9	9	24	0.1	helix	0.6	6.93	0.0002874802357337	14.5631313131313	14.5630810302277	14.5631012253236	5.81873994206135	12	8	0	8	0	0	0	0	-7	0	-3	-4	4	2	4	-0.113981762917933	-0.455297157622739	0.0323153941972188	-0.211594202898551	-0.602094240837696	0.873684210526316	-0.989291598023064	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	F	2.67848962960141	0.0202679102821363	1.15384949648317	2.3213509541454	1.86363317860665	294.488177945996	1.16325476548539	0.0174140488202037	1.75910024233227	1.16837785201846	760.989759542733	0.0158563003544608	lineage4	dr_mutations_pyrazinamide	ILE	ERR2864275	ERR2864275	lineage4.1.2.1	pt	Portugal	pnca_p.phe13ile	A	G	PHE	FA13	pnca_complex.pdb
45	F13L	L4	PZA	F	F13	13	L	1	XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	3.551	-0.427	-2.033	1.0968	-1.283	-2.06	84	91	-1.201	9	9	24	0.1	helix	0.6	6.93	0.0005749604714675	34.0379426644182	34.0375872334388	34.0436365315203	23.2409378193551	8	6	0	6	0	0	0	0	-4	0	-2	-4	-4	-2	0	-0.108156028368794	-0.525322997416021	0.0399478434429155	-0.218755328218244	-0.539267015706806	0.884210526315789	-0.989291598023064	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	F	3.52746542085928	0.0009482725856346	1.06716956280854	3.3054404321426	6.05331233477459	636.464952476757	1.53196330239691	2.88610345466244e-05	4.53968810533409	4.36820226973726	1521.29182901896	1.42922316872435e-06	lineage4	dr_mutations_pyrazinamide	LEU	ERR1873518	ERR1873518	lineage4.1.1.3	za	South Africa	pnca_p.phe13leu	A	G	PHE	FA13	pnca_complex.pdb
46	F13S	L2	PZA	F	F13	13	S	0	XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	3.551	0.218	-3.104	2.59174	-2.032	-3.54	88	91	-1.201	9	9	24	0.1	helix	0.6	6.93	0.0002874802357337	1.61648444070648	1.61648999729673	1.61641362774286	5.75148528770432e-25	44	6	0	6	0	0	-2	0	-9	0	-5	-4	-2	2	6	0.0977578475336322	-0.802067183462532	0.0943968123310922	-0.346462063086104	-0.926701570680628	0.926315789473684	-0.989291598023064	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	F	0.480257130287146	0.677247786834331	1.1538493489906	0.416221693678883	0.0799389646160496	12.6318165284589	0.208571528713835	0.527693668101443	0.277618116975238	0.0307788891197694	20.1401594005	0.999999999999395	lineage2	dr_mutations_pyrazinamide	SER	0218N-sc-2011-09-07T13:18:52Z-1276431,ERR067584	ERR067584	lineage2.2.1	ru	Russia,Samara	pnca_p.phe13ser	A	G	PHE	FA13	pnca_complex.pdb
47	F13V	L3	PZA	F	F13	13	V	0	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	3.551	-0.562	-2.57	1.40193	-2.51	-2.34	84	91	-1.201	9	9	24	0.1	helix	0.6	6.93	0.0001437401178668	1.2119588062211	4.59700131334268e-05	0	1.01056822389159e-25	44	8	0	8	2	0	2	0	-1	0	-1	0	0	-4	0	-0.142350557244174	-0.664082687338501	0.051061342230057	-0.427962489343563	-0.612565445026178	0.884210526315789	-0.989291598023064	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	147/150	F	-9.98752126245058	0.942832958100734	139.277183325397	-0.0717096729269463	NA	144471.706314842	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage3	other_mutations_pyrazinamide	VAL	ERR2510280	ERR2510280	lineage3	pk	Pakistan	pnca_p.phe13val	A	G	PHE	FA13	pnca_complex.pdb
48	C14G	L4	PZA	C	C14	14	G	0	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	non-polar	8.493	-1.214	-0.534	1.40101	-2.618	1.21	82	91	0.013	5	5	1	0.0059880239520958	loop	0.6	5.76	0.0009343107661348	4.1639877189814	4.16398768969361	4.16339464867528	5.83418569155344	10	0	0	0	0	0	2	-2	4	0	4	2	4	13	15	-0.307497467071935	-0.137984496124031	0.0510278338274609	-0.446376811594203	0.679775280898876	0.863157894736842	0.00416666666666667	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.244	0.225	6:4	147/150	L:V:C:I:M:A:T	1.42647319438274	0.0104102993024226	0.556804076010637	2.56189431047824	1.33944091330526	12.5461379903288	0.619509439957981	0.0142886927095374	1.84500750353971	1.15481122214044	14.4829286214949	0.0157176829434794	lineage4	dr_mutations_pyrazinamide	GLY	ERR2510823	ERR2510823	lineage4.8	it	Italy	pnca_p.cys14gly	A	S	CYS	CA14	pnca_complex.pdb
49	C14R	L2	PZA	C	C14	14	R	NA	XDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	8.493	-1.215	-0.333	-1.79171	-2.708	0.11	81	91	0.013	5	5	1	0.0059880239520958	loop	0.6	5.76	0.0034497628288055	42.4477739726027	42.4477736723918	42.4422996313437	173.445422378416	-58	-18	0	-18	-4	0	-2	-2	-5	0	-2	-3	0	-2	-2	-0.307750759878419	-0.0860465116279069	-0.344480119972314	-0.461722080136402	0.0617977528089887	0.852631578947368	0.00416666666666667	Destabilising	Destabilising	Stabilising	Destabilising	Stabilising	effect	-0.244	0.225	6:4	147/150	L:V:C:I:M:A:T	3.74827446550197	2.29709370000913e-15	0.473019029416408	7.92415152964658	18.4724160727947	122.828725767578	1.6278549200979	5.27268754098056e-28	27.2779679641604	16.8169087404394	137.378365631042	1.30826250297363e-39	lineage2	dr_mutations_pyrazinamide	ARG	ERR2516005	ERR2516005	lineage2.2.2	za	South Africa	pnca_p.cys14arg	A	S	CYS	CA14	pnca_complex.pdb
50	C14W	L2	PZA	C	C14	14	W	1	XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	8.493	-0.421	-1.426	3.56718	-1.443	-1.01	81	91	0.013	5	5	1	0.0059880239520958	loop	0.6	5.76	0.0002156101768003	9.70466975178797	9.70465898653051	9.70194990589383	2.29168245016684	-40	0	0	0	0	0	0	0	-2	0	-2	-4	0	0	-2	-0.106636271529888	-0.368475452196382	0.129924460405452	-0.246035805626598	-0.264397905759162	0.852631578947368	0.00416666666666667	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.225	6:4	147/150	L:V:C:I:M:A:T	2.27260607808817	0.0634455902260898	1.22442760158496	1.8560559033024	0.929172866365548	208.881603004778	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	dr_mutations_pyrazinamide	TRP	ERR1873469	ERR1873469	lineage2.2.1	za	South Africa	pnca_p.cys14trp	A	S	CYS	CA14	pnca_complex.pdb
51	C14Y	L2	PZA	C	C14	14	Y	NA	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	polar	8.493	-0.604	-0.931	-0.852908	-1.613	-0.51	73	85	0.013	5	5	1	0.0059880239520958	loop	0.6	5.76	0.0001437401178668	4.85029436501262	4.85029432717734	4.84956447904112	0.0881192621863332	-40	-2	0	-2	0	0	0	0	-5	0	-3	-2	0	0	-2	-0.152988855116515	-0.240568475452196	-0.163982927016842	-0.275021312872975	-0.133507853403141	0.768421052631579	0.00416666666666667	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.244	0.225	6:4	147/150	L:V:C:I:M:A:T	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673665	122.683760399245	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	dr_mutations_pyrazinamide	TYR	SRR5709803,SAMN07236302	SRR5709803	lineage2.2.2	th	Thailand	pnca_p.cys14tyr	A	S	CYS	CA14	pnca_complex.pdb
52	E15D	L4	PZA	E	E15	15	D	NA	Sensitive	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	11.854	0.146	-0.135	2.38936	-0.543	-0.05	-31	66	-0.898	8	8	92	0.412556053811659	loop	-0.466666666666667	3.7	NA	NA	NA	NA	NA	10	4	0	4	7	0	7	0	4	0	2	2	0	2	-4	0.0654708520179372	-0.0348837209302325	0.0870256922034688	-0.0925831202046036	-0.013089005235602	-0.333333333333333	-0.739703459637562	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.993	-0.827	9:8	146/150	H:A:S:E:P:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ASP	ERR2229487	ERR2229487	lineage4.8	dk		pnca_p.glu15asp	A	T	GLU	EA15	pnca_complex.pdb
53	E15G	L2;L4	PZA	E	E15	15	G	0	MDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	11.854	-0.81	0.063	2.82636	-0.925	0.47	66	80	-0.898	8	8	92	0.412556053811659	loop	-0.466666666666667	3.7	0.0003593502946672	0.484657419083649	1.69068633684896e-05	0	0.177777263899814	16	10	0	10	7	0	7	0	4	0	2	2	0	11	5	-0.20516717325228	0.0535714285714285	0.102942183436651	-0.15771526001705	0.264044943820225	0.694736842105263	-0.739703459637562	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.993	-0.827	9:8	146/150	H:A:S:E:P:D	-10.9877909019995	0.939691219428539	145.22979768191	-0.075657964669658	NA	60.3389082676129	-0.314565134742567	0.596111875058711	0.224672226566594	0	5.29224350433385	0.673290424524484	lineage2;lineage4	other_mutations_pyrazinamide	GLY	14MT_11622	SRR5525995	lineage4.9;lineage2.2.1.1	pg		pnca_p.glu15gly	A	T	GLU	EA15	pnca_complex.pdb
54	G17A	L2	PZA	G	G17	17	A	NA	MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	11.288	-0.016	-0.03	4.40656	-1.942	-0.17	66	80	-1.147	9	9	18	0.173076923076923	loop	-0.533333333333333	3.87	7.18700589334483e-05	9.70142977291842	188179.926264583	Inf	0.763784339928386	-6	0	0	0	2	0	2	0	-2	0	-4	2	-6	-13	-18	-0.0040526849037487	-0.0077519379844961	0.160496507113251	-0.331116794543905	-0.0445026178010471	0.694736842105263	-0.944810543657331	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	147/150	R:G	12.1451538387262	0.919026337424224	119.468044436215	0.101660271548268	5.01153666064308e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ALA	ERR718273	ERR718273	lineage2.2.1	th	Thailand	pnca_p.gly17ala	A	S	GLY	GA17	pnca_complex.pdb
55	G17D	L4	PZA	G	G17	17	D	NA	MDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	11.288	1.678	-0.552	15.5901	-2.984	-0.05	78	85	-1.147	9	9	18	0.173076923076923	loop	-0.533333333333333	3.87	0.0003593502946672	19.4256842105263	19.4256680571767	19.4169790983636	9.87561363723508	-26	-26	0	-26	-2	0	-4	-2	-6	0	-6	-2	-6	-11	-21	0.752466367713005	-0.142635658914729	0.567825377515862	-0.508780903665814	-0.013089005235602	0.821052631578947	-0.944810543657331	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	147/150	R:G	2.96659528728018	0.0079500503166054	1.11770528264822	2.65418382943607	2.87215213651461	380.0971589756	1.28837632444196	0.0036816889015929	2.43395291165754	1.92061868691892	951.403114304703	0.0016748373055023	lineage4	dr_mutations_pyrazinamide	ASP	ERR1035344	ERR1035344	lineage4.3.2	br	Brazil	pnca_p.gly17asp	A	S	GLY	GA17	pnca_complex.pdb
56	G17R	L2	PZA	G	G17	17	R	1	MDR	OM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	11.288	0.099	-0.0579999999999999	13.8675	-2.189	0.01	86	91	-1.147	9	9	18	0.173076923076923	loop	-0.533333333333333	3.87	7.18700589334483e-05	9.70142977291842	188179.926264663	Inf	0.763784339928386	-74	-16	0	-16	-2	0	-2	-2	-7	0	-7	-5	-10	-13	-21	0.0443946188340807	-0.0149870801033591	0.505084535872202	-0.373231031543052	0.0056179775280898	0.905263157894737	-0.944810543657331	Stabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.124	9:9	147/150	R:G	12.1451538387266	0.919026337424239	119.468044436241	0.10166027154825	5.0115366606189e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ARG	ERR2510655	ERR2510655	lineage2.2.1	it	Italy	pnca_p.gly17arg	A	S	GLY	GA17	pnca_complex.pdb
57	G17S	L4	PZA	G	G17	17	S	0	Pre-MDR	OM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	11.288	0.598	-0.437	8.97262	-1.946	-0.26	68	80	-1.147	9	9	18	0.173076923076923	loop	-0.533333333333333	3.87	0.0002156101768003	2.42493692178301	2.4249395742581	2.42473736947533	4.31445715363782e-25	-6	0	0	0	-2	0	-2	-2	-7	0	-7	0	-4	-11	-19	0.268161434977578	-0.112919896640827	0.32680235141573	-0.331798806479113	-0.0680628272251308	0.715789473684211	-0.944810543657331	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	147/150	R:G	0.885806606247281	0.469406216824826	1.22442753211922	0.723445514749362	0.112662937620391	25.327054747117	0.384700446081433	0.430258985168156	0.366270051463612	0.0410854444106247	46.5881472867033	0.999999999999476	lineage4	other_mutations_pyrazinamide	SER	1054X-sc-2012-02-09T18:57:40Z-1355318,ERR133985	ERR133985	lineage4.3.3	ru	Russia	pnca_p.gly17ser	A	S	GLY	GA17	pnca_complex.pdb
58	S18P	L1	PZA	S	S18	18	P	0	Other	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	9.173	-1.13	-0.521	1.09268	0.064	-0.16	53	75	-0.553	7	7	53	0.341935483870968	loop	0.866666666666667	3.72	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	0	0	0	4	0	4	0	-4	2	-6	-2	-2	-8	0	-0.286220871327254	-0.134625322997416	0.0397977840747674	0.0540540540540541	-0.0418848167539267	0.557894736842105	-0.455518945634267	Destabilising	Destabilising	Destabilising	Stabilising	Destabilising	effect	-0.674	-0.485	7:7	147/150	T:S:A:R:K:N:H:E	-8.9874087814964	0.940032862253632	119.468044435489	-0.0752285585987761	NA	4692673.51081157	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	dr_mutations_pyrazinamide	PRO	SAMN03648394,SRR2101147	SRR2101147	lineage1.2.2			pnca_p.ser18pro	A	T	SER	SA18	pnca_complex.pdb
59	L19P	L4	PZA	L	L19	19	P	1	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	5.113	-1.723	-1.791	2.98164	-1.477	-1.37	83	91	-1.173	9	9	27	0.134328358208955	loop	1.6	4.47	0.0003593502946672	19.4256842105263	19.4256680571767	19.4169790983636	9.87561363723508	26	0	0	0	0	0	0	0	7	6	-1	1	-4	-2	0	-0.436423505572442	-0.462790697674418	0.108597819040057	-0.251832907075874	-0.358638743455497	0.873684210526316	-0.966227347611203	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	L:V	2.96659528728018	0.0079500503166053	1.11770528264822	2.65418382943607	2.87215213651493	380.09715897553	1.28837632444196	0.0036816889015929	2.43395291165754	1.92061868691892	951.403114304703	0.0016748373055023	lineage4	dr_mutations_pyrazinamide	PRO	ERR2041727	ERR2041727	lineage4.4.1.1	rs	Serbia	pnca_p.leu19pro	A	T	LEU	LA19	pnca_complex.pdb
60	L19R	L3	PZA	L	L19	19	R	1	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	5.113	-0.665	-1.418	2.62574	-1.398	-0.57	81	91	-1.173	9	9	27	0.134328358208955	loop	1.6	4.47	0.0002156101768003	9.70466975178797	9.70465898653051	9.70194990589383	2.29168245016684	-54	-30	0	-30	0	0	0	0	2	0	-1	2	-4	0	-8	-0.168439716312057	-0.36640826873385	0.0956351663400812	-0.238363171355499	-0.149214659685864	0.852631578947368	-0.966227347611203	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	L:V	2.27260607808817	0.0634455902260897	1.22442760158496	1.8560559033024	0.929172866364976	208.881603004981	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage3	dr_mutations_pyrazinamide	ARG	ERR3335797	ERR3335797	lineage3	pk	Pakistan	pnca_p.leu19arg	A	T	LEU	LA19	pnca_complex.pdb
61	L19V	L4	PZA	L	L19	19	V	NA	Pre-MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.113	-1.722	-2.048	1.25265	-1.307	-2.23	7	53	-1.173	9	9	27	0.134328358208955	loop	1.6	4.47	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	26	0	0	0	0	0	0	0	-2	0	-2	-1	-2	0	-2	-0.436170212765957	-0.529198966408269	0.0456242396870607	-0.222847399829497	-0.583769633507853	0.0736842105263158	-0.966227347611203	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	L:V	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987759	NA	4692673.51081035	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	VAL	SRR6325448	SRR6325448	lineage4.3	us		pnca_p.leu19val	A	T	LEU	LA19	pnca_complex.pdb
62	A20P	L3;L4	PZA	A	A20	20	P	NA	Pre-MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	9.104	-0.318	-0.313	-1.37798	-0.07	0.06	-76	87	-0.39	6	6	52	0.403100775193798	loop	3.26666666666667	3.81	NA	NA	NA	NA	NA	0	0	0	0	0	0	0	0	-4	2	-6	0	0	0	-3	-0.080547112462006	-0.0808785529715762	-0.264935014996539	-0.0119352088661552	0.0337078651685393	-0.817204301075269	-0.321252059308072	Destabilising	Destabilising	Stabilising	Destabilising	Stabilising	neutral	-0.553	-0.332	7:6	147/150	P:A:S:Y:E:G:H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage3;lineage4	other_mutations_pyrazinamide	PRO	SRR6325444	SRR6325444	lineage4.3;lineage3	us		pnca_p.ala20pro	A	-	ALA	AA20	pnca_complex.pdb
63	A20S	L2	PZA	A	A20	20	S	NA	Other	OM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	9.104	0.467	-0.846	-0.127919	-0.376	-0.16	-46	72	-0.39	6	6	52	0.403100775193798	loop	3.26666666666667	3.81	NA	NA	NA	NA	NA	0	0	0	0	-4	0	-4	0	-3	0	-3	0	0	0	-1	0.209417040358744	-0.218604651162791	-0.0245941321233561	-0.0641091219096334	-0.0418848167539267	-0.494623655913978	-0.321252059308072	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.553	-0.332	7:6	147/150	P:A:S:Y:E:G:H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	SER	ERR502943	ERR502943	lineage2.2			pnca_p.ala20ser	A	-	ALA	AA20	pnca_complex.pdb
64	V21A	L4	PZA	V	V21	21	A	1	XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	6.521	-0.327	-1.742	1.43401	-1.866	-1.79	60	80	-1.104	9	9	9	0.0517241379310344	loop	1.76666666666667	4.94	0.0001437401178668	4.85029436501262	4.85029432717736	4.84956447904112	0.0881192621863332	4	0	0	0	2	0	0	0	-1	0	-1	0	2	4	1	-0.0828267477203647	-0.450129198966408	0.0522297656597149	-0.318158567774936	-0.468586387434555	0.631578947368421	-0.909390444810544	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	147/150	T:V:C:I	1.57903938912402	0.264247566376722	1.41439286276584	1.11640791656443	0.191756076673664	122.683760399233	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage4	dr_mutations_pyrazinamide	ALA	SAMN02419617,XTB13-183	SRR1172308_SRR1162962_SRR1168981_SRR1166910	lineage4.3.3	by	Belarus	pnca_p.val21ala	A	-	VAL	VA21	pnca_complex.pdb
65	V21G	L3	PZA	V	V21	21	G	1	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	6.521	-0.391	-2.004	2.37287	-2.469	-2.8	70	85	-1.104	9	9	9	0.0517241379310344	loop	1.76666666666667	4.94	0.0004312203536006	24.2923336141533	24.29233342416	24.2833096922897	14.1977442691074	16	0	0	0	0	0	0	0	0	0	0	0	2	11	15	-0.0990374873353596	-0.517829457364341	0.0864250905091092	-0.420971867007673	-0.732984293193717	0.736842105263158	-0.909390444810544	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	147/150	T:V:C:I	3.19016080361872	0.0035954281300594	1.09565968954933	2.91163472932996	3.91551977432241	465.531063220335	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage3	dr_mutations_pyrazinamide	GLY	ERR550764,SAMEA2533452	ERR550764	lineage3.1.1			pnca_p.val21gly	A	-	VAL	VA21	pnca_complex.pdb
66	V21I	L4	PZA	V	V21	21	I	NA	Other	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	6.521	-0.188	-0.748	-0.568333	-0.271	-0.56	30	66	-1.104	9	9	9	0.0517241379310344	loop	1.76666666666667	4.94	NA	NA	NA	NA	NA	-14	0	0	0	0	0	0	0	-2	0	-2	0	0	-2	-2	-0.0476190476190476	-0.19328165374677	-0.1092695916327	-0.0462063086104007	-0.146596858638743	0.315789473684211	-0.909390444810544	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	147/150	T:V:C:I	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ILE	ERR1034923	ERR1034923	lineage4.1.2.1	za	South Africa	pnca_p.val21ile	A	-	VAL	VA21	pnca_complex.pdb
67	T22A	L4	PZA	T	T22	22	A	NA	Pre-MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	11.195	-0.361	0.118	-0.193269	0.057	-0.37	-93	97	1.546	1	1	127	0.738372093023256	loop	1.03333333333333	3.6	NA	NA	NA	NA	NA	0	0	0	0	0	0	0	0	0	0	0	0	2	4	1	-0.0914387031408308	0.100340136054422	-0.0371585403368453	0.0481418918918919	-0.0968586387434555	-1	0.495512820512821	Destabilising	Stabilising	Stabilising	Stabilising	Destabilising	neutral	0.842	1.712	2:1	147/150	T:A:S:G:P:D:H:E:Q:N:K:I:R	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ALA	SRR6325445	SRR6325445	lineage4.3	us		pnca_p.thr22ala	A	T	THR	TA22	pnca_complex.pdb
68	T22P	L2	PZA	T	T22	22	P	NA	Other	OM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	11.195	-0.353	0.063	-1.50027	0.006	0	-77	87	1.546	1	1	127	0.738372093023256	loop	1.03333333333333	3.6	NA	NA	NA	NA	NA	0	0	0	0	0	0	0	0	0	0	0	0	0	0	-4	-0.0894123606889564	0.0535714285714285	-0.288446896869953	0.00506756756756757	0	-0.827956989247312	0.495512820512821	Destabilising	Stabilising	Stabilising	Stabilising	Stabilising	neutral	0.842	1.712	2:1	147/150	T:A:S:G:P:D:H:E:Q:N:K:I:R	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	PRO	ERR502943	ERR502943	lineage2.2			pnca_p.thr22pro	A	T	THR	TA22	pnca_complex.pdb
69	G23V	L2	PZA	G	G23	23	V	0	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	11.963	-0.147	-0.781	2.85361	-2.833	-1.41	53	75	0.492	3	3	28	0.269230769230769	loop	-0.5	3.83	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-2	0	0	0	0	0	0	0	-3	0	-3	0	-2	-5	-13	-0.0372340425531914	-0.201808785529716	0.103934687752679	-0.483034953111679	-0.369109947643979	0.557894736842105	0.157692307692308	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.083	0.59	5:3	147/150	G:D:H:Y:E:Q:N:R:K	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987751	NA	4692673.51081314	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	dr_mutations_pyrazinamide	VAL	MDR_27149	SRR6824547	lineage2.2.1	cn	China: Weiyang District, Xi'an City, Shaanxi Province	pnca_p.gly23val	A	T	GLY	GA23	pnca_complex.pdb
70	G24D	L4	PZA	G	G24	24	D	0	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	10.19	1.138	-2.197	4.32448	-3.803	-1.77	80	91	-0.971	9	9	0	0	helix	0.333333333333333	5.96	0.0005030904125341	29.1630847029077	29.1630732435189	29.1293325978315	18.6717493808583	-20	-28	0	-28	0	0	0	0	-10	0	-6	-6	-6	-9	-23	0.510313901345291	-0.567700258397933	0.157506974846845	-0.648422847399829	-0.463350785340314	0.842105263157895	-0.799835255354201	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.094	-0.914	9:8	147/150	G:S:A	3.37290329402598	0.001789077082818	1.07995768762031	3.12318096596746	4.97876379054099	550.894323519243	1.46483345924118	0.0001485295621003	3.828187099348	3.53443753556636	1331.56873199938	1.55265977742841e-05	lineage4	dr_mutations_pyrazinamide	ASP	ERR2041731	ERR2041731	lineage4.2.2.1	rs	Serbia	pnca_p.gly24asp	A	H	GLY	GA24	pnca_complex.pdb
71	G24R	L2	PZA	G	G24	24	R	NA	XDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	10.19	-0.978	-1.19	6.83709	-3.44	-0.67	82	91	-0.971	9	9	0	0	helix	0.333333333333333	5.96	7.18700589334483e-05	9.70142977291842	188179.926264595	Inf	0.763784339928386	-64	-30	0	-30	-2	0	0	-2	-9	0	-3	-11	-14	-17	-29	-0.247720364741641	-0.30749354005168	0.249021700332899	-0.586530264279625	-0.175392670157068	0.863157894736842	-0.799835255354201	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.094	-0.914	9:8	147/150	G:S:A	12.1451538387262	0.919026337424226	119.468044436218	0.101660271548266	5.01153666064e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	ARG	SAMN02628481	SRR1174894_SRR1183028_SRR1172289_SRR1180230	lineage2.2.1	ir	Iran	pnca_p.gly24arg	A	H	GLY	GA24	pnca_complex.pdb
72	G24S	L2	PZA	G	G24	24	S	1	XDR	OM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	10.19	-0.034	-1.524	-0.205598	-2.078	-1.03	41	71	-0.971	9	9	0	0	helix	0.333333333333333	5.96	7.18700589334483e-05	9.70142977291842	188179.926264687	Inf	0.763784339928386	-8	0	0	0	-2	0	-2	0	-8	0	-6	-4	-4	-9	-17	-0.008611955420466	-0.393798449612403	-0.0395289548565716	-0.354305200341006	-0.269633507853403	0.431578947368421	-0.799835255354201	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.094	-0.914	9:8	147/150	G:S:A	12.1451538387267	0.919026337424243	119.468044436249	0.101660271548244	5.01153666061174e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	SER	ERR2516425	ERR2516425	lineage2.2.1	pe	Peru	pnca_p.gly24ser	A	H	GLY	GA24	pnca_complex.pdb
73	G24V	L2	PZA	G	G24	24	V	0	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	10.19	-1.361	-0.186	2.33197	-2.591	-1.1	70	85	-0.971	9	9	0	0	helix	0.333333333333333	5.96	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-8	0	0	0	0	0	0	0	-9	0	-7	-6	-6	-11	-23	-0.344731509625127	-0.0480620155038759	0.0849354234806489	-0.441773231031543	-0.287958115183246	0.736842105263158	-0.799835255354201	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.094	-0.914	9:8	147/150	G:S:A	-8.98740878149641	0.940032862253632	119.46804443549	-0.0752285585987761	NA	4692673.51081173	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	VAL	ERR1035102	ERR1035102	lineage2.2.1	vn	Vietnam	pnca_p.gly24val	A	H	GLY	GA24	pnca_complex.pdb
74	A25V	L4	PZA	A	A25	25	V	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	12.121	-0.995	-0.514	-0.280715	-0.778	-0.65	-66	82	-0.242	6	6	26	0.201550387596899	helix	1.06666666666667	4.5	NA	NA	NA	NA	NA	-2	0	0	0	0	0	2	-2	1	0	3	-1	0	0	3	-0.252026342451874	-0.1328165374677	-0.0539711989540875	-0.132651321398124	-0.170157068062827	-0.709677419354839	-0.199341021416804	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.411	-0.147	7:6	147/150	R:K:N:H:E:Y:D:T:S:G:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	VAL	ERR3275947	ERR3275947	lineage4.2.2	nl	Netherlands	pnca_p.ala25val	A	H	ALA	AA25	pnca_complex.pdb
75	L27P	L4	PZA	L	L27	27	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	9.548	-1.558	-1.624	4.6599	-3.919	-1.07	71	85	-0.385	6	6	11	0.0547263681592039	helix	2.46666666666667	5.14	0.0007187005893344	19.4567692956558	19.4567531291168	19.4480067941443	23.6697146258566	22	0	0	0	2	2	0	0	-2	4	-4	-2	2	6	2	-0.394630192502533	-0.419638242894057	0.169723701367289	-0.668201193520887	-0.280104712041885	0.747368421052632	-0.317133443163097	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.332	7:6	147/150	R:C:I:V:L:T:M	2.96819421438473	0.000173451696941	0.790498020917702	3.75484079130129	4.8718075812224	128.958907982956	1.28907072937786	2.34381123661905e-05	4.63007736799346	3.87828374077737	187.779865627687	1.14368245582331e-06	lineage4	dr_mutations_pyrazinamide	PRO	SAMN02567730	SRR1166108	lineage4.2.1	ro	Romania	pnca_p.leu27pro	A	H	LEU	LA27	pnca_complex.pdb
76	L27Q	L4	PZA	L	L27	27	Q	NA	MDR	OM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	9.548	0.134	-1.081	2.24048	-3.309	-0.81	45	71	-0.385	6	6	11	0.0547263681592039	helix	2.46666666666667	5.14	NA	NA	NA	NA	NA	-8	0	0	0	-2	0	-2	0	-10	0	-6	-4	2	2	-2	0.0600896860986547	-0.279328165374677	0.0816031585311665	-0.564194373401535	-0.212041884816754	0.473684210526316	-0.317133443163097	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.332	7:6	147/150	R:C:I:V:L:T:M	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLN	SAMN02628478	SRR1174323_SRR1172944	lineage4.4	ir	Iran	pnca_p.leu27gln	A	H	LEU	LA27	pnca_complex.pdb
77	L27R	L4	PZA	L	L27	27	R	1	Pre-XDR	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	9.548	-1.016	-0.738	2.15593	-3.558	-0.1	69	80	-0.385	6	6	11	0.0547263681592039	helix	2.46666666666667	5.14	0.0001437401178668	4.85029436501262	4.85029432717734	4.84956447904112	0.0881192621863332	-46	-20	-2	-18	0	0	-2	0	-3	0	-3	-1	0	2	4	-0.257345491388045	-0.190697674418605	0.0785236634882247	-0.606649616368286	-0.0261780104712041	0.726315789473684	-0.317133443163097	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.332	7:6	147/150	R:C:I:V:L:T:M	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673649	122.683760399218	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage4	other_mutations_pyrazinamide	ARG	ERR2516687	ERR2516687	lineage4.3.4.2	pe	Peru	pnca_p.leu27arg	A	H	LEU	LA27	pnca_complex.pdb
78	A28D	L2	PZA	A	A28	28	D	1	MDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	12.7	0.554	-2.8	3.01687	-4.44	-1.38	67	80	-0.564	7	7	0	0	helix	0.366666666666667	6.48	7.18700589334483e-05	9.70142977291842	188179.926264663	Inf	0.763784339928386	-8	-52	0	-52	0	0	4	-4	-7	0	-3	-2	-8	-10	-5	0.248430493273542	-0.723514211886305	0.10988097232643	-0.757033248081841	-0.361256544502618	0.705263157894737	-0.464579901153213	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	147/150	P:A:I:V:L	12.1451538387266	0.91902633742424	119.468044436242	0.101660271548249	5.01153666061778e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	ASP	SAMN10961375	SRR8651616	lineage2.2.1	cn	China:Wenzhou	pnca_p.ala28asp	A	H	ALA	AA28	pnca_complex.pdb
79	A28T	L4	PZA	A	A28	28	T	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	12.7	-0.289	-1.901	-0.611286	-2.577	-1.74	41	71	-0.564	7	7	0	0	helix	0.366666666666667	6.48	NA	NA	NA	NA	NA	-2	0	0	0	-2	0	-2	0	-8	0	-2	-6	-8	-4	-5	-0.0732016210739615	-0.491214470284238	-0.117527878181958	-0.439386189258312	-0.455497382198953	0.431578947368421	-0.464579901153213	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	147/150	P:A:I:V:L	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	THR	ERR2516621	ERR2516621	lineage4.1.1.3	nl	Netherlands	pnca_p.ala28thr	A	H	ALA	AA28	pnca_complex.pdb
80	A30V	L1	PZA	A	A30	30	V	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	16.46	-0.846	-0.401	-0.154342	-0.442	-0.7	-76	87	1.207	1	1	37	0.286821705426357	helix	0.6	3.88	7.18700589334483e-05	9.70142977291842	188179.9262646	Inf	0.763784339928386	-4	0	0	0	0	0	0	0	-5	0	-5	-4	0	0	-3	-0.214285714285714	-0.103617571059432	-0.0296743059294009	-0.0753623188405797	-0.183246073298429	-0.817204301075269	0.386858974358974	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	0.59	1.183	3:1	147/150	Q:E:Y:K:I:R:V:L:N:P:M:A:G:T:D	12.1451538387263	0.919026337424228	119.468044436221	0.101660271548264	5.01153666063734e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage1	other_mutations_pyrazinamide	VAL	ERR3256161	ERR3256161	lineage1.2.1	ph	Philippines	pnca_p.ala30val	A	H	ALA	AA30	pnca_complex.pdb
81	I31S	L2	PZA	I	I31	31	S	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	11.654	-0.329	-3.608	4.81248	-3.395	-3.34	60	80	-0.562	7	7	0	0	helix	1.83333333333333	6.99	0.0004312203536006	58.3312262958281	1393450.94022095	Inf	23.547488635408	28	0	0	0	0	0	-2	0	0	0	0	0	2	2	2	-0.0833333333333333	-0.932299741602067	0.17528099709351	-0.578857630008525	-0.87434554973822	0.631578947368421	-0.462932454695222	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	147/150	I:L:V:T:A	14.1472939191279	0.915018435774967	132.576060676205	0.106710773023195	4.17896182050295	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage2	dr_mutations_pyrazinamide	SER	ETB0068	SRR6257006	lineage2.2.1	ru	Russia:Altaysky Krai	pnca_p.ile31ser	A	H	ILE	IA31	pnca_complex.pdb
82	I31T	L2	PZA	I	I31	31	T	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	11.654	-0.358	-3.12	3.06922	-3.102	-3.05	62	80	-0.562	7	7	0	0	helix	1.83333333333333	6.99	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	24	0	0	0	-2	0	-2	0	-5	0	-5	0	2	2	0	-0.0906788247213779	-0.806201550387597	0.111787673278506	-0.528900255754476	-0.798429319371728	0.652631578947368	-0.462932454695222	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	147/150	I:L:V:T:A	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987758	NA	4692673.51081066	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	THR	SAMN07236365,SRR5709965	SRR5709965	lineage2.2.1	th	Thailand	pnca_p.ile31thr	A	H	ILE	IA31	pnca_complex.pdb
83	S32G	L1	PZA	S	S32	32	G	NA	Sensitive	OM	hydrophilic	hydrophobic	neutral	neutral	polar	non-polar	16.787	-1.334	-0.875	0.0485615999999999	-0.992	-0.43	-32	66	-0.647	7	7	7	0.0451612903225806	loop	0.0666666666666666	4.62	NA	NA	NA	NA	NA	4	0	0	0	2	0	4	0	3	0	3	0	2	5	17	-0.337892603850051	-0.22609819121447	0.00176871917773293	-0.169138959931799	-0.112565445026178	-0.344086021505376	-0.532948929159802	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.779	-0.553	8:7	147/150	D:T:S:G:A:V:N:R:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	GLY	SRR5067283	SRR5067283	lineage1.1.1	vn	Vietnam	pnca_p.ser32gly	A	T	SER	SA32	pnca_complex.pdb
84	S32I	L4	PZA	S	S32	32	I	1	Pre-XDR	DM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	16.787	-1.207	0.018	0.86851	-0.677	-0.41	29	63	-0.647	7	7	7	0.0451612903225806	loop	0.0666666666666666	4.62	7.18700589334483e-05	9.70142977291842	188179.926264664	Inf	0.763784339928386	-10	0	0	0	2	0	4	0	0	0	-2	-3	0	0	0	-0.305724417426545	0.0153061224489795	0.0316330247160891	-0.115430520034101	-0.107329842931937	0.305263157894737	-0.532948929159802	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.553	8:7	147/150	D:T:S:G:A:V:N:R:Q	12.1451538387266	0.919026337424239	119.468044436241	0.101660271548249	5.01153666061844e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	ILE	ERS458092	ERR552499_ERR552498	lineage4.3.3	uz	Nukus	pnca_p.ser32ile	A	T	SER	SA32	pnca_complex.pdb
85	D33V	L4	PZA	D	D33	33	V	1	Other	OM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	20.313	-0.795	0.276	1.48114	-0.022	-0.3	-5	53	1.213	1	1	77	0.398963730569948	loop	-1.86666666666667	3.72	7.18700589334483e-05	9.70142977291842	188179.9262646	Inf	0.763784339928386	4	14	2	12	0	0	0	0	0	0	0	0	0	0	-2	-0.201367781155015	0.23469387755102	0.053946342849234	-0.00375106564364876	-0.0785340314136125	-0.0537634408602151	0.388782051282051	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	neutral	0.842	1.183	2:1	147/150	N:L:V:K:R:H:E:Q:D:T:A:S:G	12.1451538387263	0.919026337424228	119.468044436221	0.101660271548264	5.01153666063734e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	VAL	ERS457272	ERR551343_ERR551342	lineage4.1.2.1	uz	Nukus	pnca_p.asp33val	A	T	ASP	DA33	pnca_complex.pdb
86	Y34C	L4	PZA	Y	Y34	34	C	NA	MDR	OM	hydrophobic	hydrophobic	neutral	neutral	polar	non-polar	18.259	-0.635	-1.829	1.92562	-0.861	-1.94	-2	53	0.42	3	3	26	0.0988593155893536	loop	-0.333333333333333	4.65	NA	NA	NA	NA	NA	48	10	2	8	-2	0	-4	2	2	0	0	2	6	2	6	-0.160840932117528	-0.472609819121447	0.0701352719643937	-0.146803069053708	-0.507853403141361	-0.021505376344086	0.134615384615385	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.083	0.59	5:3	147/150	M:A:W:Y:E:F:H:Q:V:L:R:C:I	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	CYS	SAMN02231172	SRR998793_SRR998792_SRR998795_SRR998794	lineage4.1	ml	Mali	pnca_p.tyr34cys	A	T	TYR	YA34	pnca_complex.pdb
87	Y34D	L4	PZA	Y	Y34	34	D	NA	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	acidic	polar	neg	18.259	0.124	-1.857	2.0881	-1.484	-1.99	30	66	0.42	3	3	26	0.0988593155893536	loop	-0.333333333333333	4.65	0.0005749604714675	14.5802781289507	14.5802277940117	14.5801759034061	15.0663448164144	38	-18	0	-18	2	0	0	2	3	0	0	3	8	2	6	0.0556053811659192	-0.47984496124031	0.0760531472402917	-0.253026427962489	-0.520942408376963	0.315789473684211	0.134615384615385	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.083	0.59	5:3	147/150	M:A:W:Y:E:F:H:Q:V:L:R:C:I	2.6796663501904	0.0010245352377104	0.816050632122416	3.28370108999367	3.35697961122125	99.553869259241	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage4	dr_mutations_pyrazinamide	ASP	SAMN02350491	SRR1019165_SRR1140957	lineage4.4.1.1	za	South Africa	pnca_p.tyr34asp	A	T	TYR	YA34	pnca_complex.pdb
88	Y34S	L2	PZA	Y	Y34	34	S	0	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	18.259	-0.316	-3.224	0.32836	-1.692	-3.31	13	59	0.42	3	3	26	0.0988593155893536	loop	-0.333333333333333	4.65	0.0001437401178668	1.2119588062211	4.5970013133425e-05	0	1.01056822389159e-25	48	10	2	8	-2	0	-4	2	2	0	0	2	10	2	10	-0.0800405268490375	-0.833074935400517	0.0119595859526949	-0.28849104859335	-0.866492146596859	0.136842105263158	0.134615384615385	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.083	0.59	5:3	147/150	M:A:W:Y:E:F:H:Q:V:L:R:C:I	-9.98752126245062	0.942832958100734	139.277183325398	-0.071709672926946	NA	144471.706314878	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	dr_mutations_pyrazinamide	SER	ERR2510443	ERR2510443	lineage2.2.1	pk	Pakistan	pnca_p.tyr34ser	A	T	TYR	YA34	pnca_complex.pdb
89	L35M	L4	PZA	L	L35	35	M	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	17.42	-1.12	-1.162	-0.61263	-0.928	-0.38	-4	53	0.183	4	4	35	0.174129353233831	loop	1.43333333333333	4.69	NA	NA	NA	NA	NA	-10	0	0	0	0	0	0	0	0	0	-1	0	2	-2	2	-0.283687943262411	-0.300258397932817	-0.11778628008921	-0.158226768968457	-0.0994764397905759	-0.043010752688172	0.0586538461538462	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.038	0.389	5:4	147/150	G:A:S:M:T:Q:V:L:I	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	MET	SAMN02231181	SRR1162468_SRR1162694_SRR1103486	lineage4.1	ml	Mali	pnca_p.leu35met	A	T	LEU	LA35	pnca_complex.pdb
90	L35P	L2	PZA	L	L35	35	P	0	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	17.42	-0.954	-2.054	8.91998	-2.108	-1.48	82	91	0.183	4	4	35	0.174129353233831	loop	1.43333333333333	4.69	0.0005749604714675	4.85515789473684	4.85515785897614	4.85442845338892	4.01139112494925	24	0	0	0	0	0	0	0	0	2	-5	1	8	0	8	-0.241641337386018	-0.530749354005168	0.324885088032401	-0.359420289855072	-0.387434554973822	0.863157894736842	0.0586538461538462	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	147/150	G:A:S:M:T:Q:V:L:I	1.58004161593687	0.0255237229775361	0.70746570614631	2.23338262506551	1.14726850666833	20.5466864308597	0.686203358177678	0.033386534669467	1.47642865587913	0.903495684237988	26.0627135254866	0.0451938468216249	lineage2	dr_mutations_pyrazinamide	PRO	ERR2513880	ERR2513880	lineage2.2.1	gb	UK	pnca_p.leu35pro	A	T	LEU	LA35	pnca_complex.pdb
91	L35R	L4	PZA	L	L35	35	R	NA	Sensitive	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	17.42	-0.653	-1.493	-2.11958	-2.016	-0.88	64	80	0.183	4	4	35	0.174129353233831	loop	1.43333333333333	4.69	0.0025873221216041	0.284614813531639	0.28461481380629	0.284630509244736	2.62510215004498	-30	-32	-2	-30	-2	0	-2	0	-6	0	-6	-2	-2	-8	-2	-0.165400202634245	-0.38578811369509	-0.40751749596247	-0.343734015345269	-0.230366492146597	0.673684210526316	0.0586538461538462	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	147/150	G:A:S:M:T:Q:V:L:I	-1.25661854335884	0.0842996990557667	0.727940577686249	-1.72626527752167	0.0461651045270816	0.934717733973448	-0.545742499657136	0.0746087863378038	1.1272100246275	0.033101827382896	1.11162498496942	0.105185735611338	lineage4	other_mutations_pyrazinamide	ARG	016-1	ERR1750903	lineage4.9	za		pnca_p.leu35arg	A	T	LEU	LA35	pnca_complex.pdb
92	E37V	L4	PZA	E	E37	37	V	0	Sensitive	OM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	24.08	-0.519	-0.033	0.085964	-0.065	-0.26	-32	66	2.736	1	1	138	0.618834080717489	loop	0.0333333333333333	3.58	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	12	18	2	16	2	0	2	0	-1	0	-2	1	2	2	-2	-0.131458966565349	-0.0085271317829457	0.00313099600084499	-0.0110826939471441	-0.0680628272251308	-0.344086021505376	0.876923076923077	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	1.183	3.125	1:1	95/150	F:E:Q:V:N:L:I:R:T:W:H:Y:M:G:A:S:P:D	-8.9874087814964	0.940032862253632	119.468044435489	-0.0752285585987761	NA	4692673.51081157	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	VAL	SAMN03647581,SRR2100408	SRR2100408	lineage4.1.1.1			pnca_p.glu37val	A	S	GLU	EA37	pnca_complex.pdb
93	Y41C	L4	PZA	Y	Y41	41	C	0	MDR	OM	hydrophobic	hydrophobic	neutral	neutral	polar	non-polar	19.063	-1.07	-1.057	4.03027	-1.836	0.19	37	66	-0.477	7	7	35	0.133079847908745	loop	-2.66666666666667	4.97	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	46	8	0	8	2	0	-2	4	2	0	-2	4	10	2	10	-0.271023302938197	-0.27312661498708	0.146791206229649	-0.31304347826087	0.106741573033708	0.389473684210526	-0.392915980230643	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.674	-0.411	7:7	147/150	L:N:R:F:H:Y:W:A:G	-8.98740878149643	0.940032862253634	119.468044435493	-0.0752285585987739	NA	4692673.51081287	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	CYS	ERR2510731	ERR2510731	lineage4.1.2.1	it	Italy	pnca_p.tyr41cys	A	-	TYR	YA41	pnca_complex.pdb
94	Y41D	L4	PZA	Y	Y41	41	D	1	Pre-MDR	OM	hydrophobic	hydrophilic	neutral	acidic	polar	neg	19.063	-0.298	-2.11	5.35024	-3.198	-1.85	70	85	-0.477	7	7	35	0.133079847908745	loop	-2.66666666666667	4.97	7.18700589334483e-05	9.70142977291842	188179.926264407	Inf	0.763784339928386	36	-28	-4	-24	0	0	-2	2	2	0	-2	4	4	0	4	-0.0754812563323201	-0.545219638242894	0.194867386854508	-0.545268542199489	-0.484293193717278	0.736842105263158	-0.392915980230643	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	147/150	L:N:R:F:H:Y:W:A:G	12.1451538387252	0.919026337424193	119.468044436159	0.101660271548308	5.01153666069528e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	ASP	SRR2469373,SAMN04099730	SRR2469373	lineage4.1.1.3			pnca_p.tyr41asp	A	-	TYR	YA41	pnca_complex.pdb
95	H43D	L3	PZA	H	H43	43	D	0	Pre-XDR	OM	hydrophobic	hydrophilic	basic	acidic	pos	neg	19.543	-0.912	-1.596	3.54222	-0.971	-1.78	27	63	1.071	1	1	40	0.178571428571428	strand	-0.733333333333333	4.75	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	6	-24	-2	-22	0	0	2	0	-2	0	-2	-6	0	0	0	-0.231003039513678	-0.412403100775194	0.129015362874147	-0.165558397271952	-0.465968586387435	0.284210526315789	0.343269230769231	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.59	1.183	3:1	147/150	Y:F:H:Q:L:N:V:R:I:C:A:M:T:P:D	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987746	NA	4692673.51081392	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	ASP	ERR2510343	ERR2510343	lineage3	pk	Pakistan	pnca_p.his43asp	A	E	HIS	HA43	pnca_complex.pdb
96	H43P	L4	PZA	H	H43	43	P	NA	MDR	DM	hydrophobic	hydrophilic	basic	non-polar	pos	non-polar	19.543	-1.878	-0.892	2.10447	-1.154	0.4	30	66	1.071	1	1	40	0.178571428571428	strand	-0.733333333333333	4.75	NA	NA	NA	NA	NA	18	0	0	0	2	0	0	2	-4	2	-3	-4	4	4	-4	-0.475683890577508	-0.230490956072351	0.0766493782734431	-0.196760443307758	0.224719101123595	0.315789473684211	0.343269230769231	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.59	1.183	3:1	147/150	Y:F:H:Q:L:N:V:R:I:C:A:M:T:P:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	PRO	SAMN02414879	SRR1162792_SRR1166871_SRR1144753_SRR1146380	lineage4.6.1.2	se	Sweden	pnca_p.his43pro	A	E	HIS	HA43	pnca_complex.pdb
97	H43Y	L1	PZA	H	H43	43	Y	NA	Sensitive	OM	hydrophobic	hydrophobic	basic	neutral	pos	polar	19.543	-1.341	0.984	-0.653353	-0.455	1.37	-33	66	1.071	1	1	40	0.178571428571428	strand	-0.733333333333333	4.75	NA	NA	NA	NA	NA	-22	-6	-2	-4	0	0	-2	2	-1	0	-2	0	4	-2	2	-0.339665653495441	0.836734693877551	-0.125615819426286	-0.0775788576300085	0.769662921348315	-0.354838709677419	0.343269230769231	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	neutral	0.59	1.183	3:1	147/150	Y:F:H:Q:L:N:V:R:I:C:A:M:T:P:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	TYR	ERR3276024	ERR3276024	lineage1.2.1	nl	Netherlands	pnca_p.his43tyr	A	E	HIS	HA43	pnca_complex.pdb
98	V44A	L4	PZA	V	V44	44	A	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	17.073	-1.246	-2.343	3.40395	-1.839	-2.01	57	75	-0.194	6	6	13	0.0747126436781609	strand	1.73333333333333	5.57	7.18700589334483e-05	9.70142977291842	188179.926264644	Inf	0.763784339928386	2	0	0	0	0	0	0	0	0	0	0	0	0	2	1	-0.315602836879433	-0.605426356589147	0.123979268497002	-0.313554987212276	-0.526178010471204	0.6	-0.159802306425041	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	149/150	T:A:Q:C:R:I:L:V	12.1451538387265	0.919026337424235	119.468044436235	0.101660271548254	5.01153666062486e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	ALA	ERR1035326	ERR1035326	lineage4.7	br	Brazil	pnca_p.val44ala	A	E	VAL	VA44	pnca_complex.pdb
99	V44G	L2	PZA	V	V44	44	G	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	17.073	-1.136	-2.975	5.19527	-3.88	-2.43	74	85	-0.194	6	6	13	0.0747126436781609	strand	1.73333333333333	5.57	0.0005030904125341	12.1451137320977	12.1450773794064	12.1416664151504	11.0019205274607	8	0	0	0	0	0	0	0	-2	0	-2	0	4	5	19	-0.28774062816616	-0.768733850129199	0.18922304212589	-0.6615515771526	-0.636125654450262	0.778947368421053	-0.159802306425041	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	149/150	T:A:Q:C:R:I:L:V	2.4969239337312	0.0028293218857323	0.83629495944555	2.98569769616526	2.61610833082893	84.8371270838078	1.08440158608155	0.0022516005975125	2.64750864476206	1.98635523014012	127.719946645636	0.0009101752788745	lineage2	dr_mutations_pyrazinamide	GLY	SAMN03653308,SRS935929	SRR2024890	lineage2.2.1.1	cn	China	pnca_p.val44gly	A	E	VAL	VA44	pnca_complex.pdb
100	V45A	L2	PZA	V	V45	45	A	0	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.521	-2.228	-2.783	3.60576	-1.973	-2.23	39	66	-0.375	6	6	0	0	strand	3.4	8.66	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	6	2	0	2	0	0	0	0	2	0	0	1	2	0	7	-0.564336372847011	-0.719121447028424	0.131329627983887	-0.336402387041773	-0.583769633507853	0.410526315789474	-0.308896210873147	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.244	7:6	149/150	F:A:Y:M:I:V:L	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987758	NA	4692673.5108122	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	ALA	SAMEA2534091,ERS457493	ERR551648_ERR551649	lineage2.2.1	uz	Nukus	pnca_p.val45ala	A	E	VAL	VA45	pnca_complex.pdb
101	V45E	L2	PZA	V	V45	45	E	NA	Pre-XDR	OM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	13.521	-1.593	-3.389	4.70839	-4.817	-1.92	80	91	-0.375	6	6	0	0	strand	3.4	8.66	NA	NA	NA	NA	NA	-32	-34	-2	-32	0	0	0	0	2	0	2	-1	0	0	0	-0.403495440729483	-0.875710594315245	0.171489812717167	-0.821312872975277	-0.50261780104712	0.842105263157895	-0.308896210873147	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.244	7:6	149/150	F:A:Y:M:I:V:L	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLU	SAMN02360628	SRR1003108_SRR1003107	lineage2.2.1	kr	South Korea	pnca_p.val45glu	A	E	VAL	VA45	pnca_complex.pdb
102	V45G	L3	PZA	V	V45	45	G	0	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	13.521	-1.938	-3.523	5.16465	-3.509	-3.55	80	91	-0.375	6	6	0	0	strand	3.4	8.66	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	10	2	0	2	0	0	0	0	4	0	2	1	4	5	25	-0.490881458966565	-0.910335917312662	0.188107795074265	-0.598294970161978	-0.929319371727749	0.842105263157895	-0.308896210873147	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.244	7:6	149/150	F:A:Y:M:I:V:L	-8.98740878149642	0.940032862253634	119.468044435493	-0.0752285585987741	NA	4692673.51081481	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	dr_mutations_pyrazinamide	GLY	ERR2510298	ERR2510298	lineage3	pk	Pakistan	pnca_p.val45gly	A	E	VAL	VA45	pnca_complex.pdb
103	A46E	L2	PZA	A	A46	46	E	1	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	11.322	-1.749	-1.747	2.18089	-5.096	-1.21	79	85	-0.822	8	8	0	0	strand	1.76666666666667	8.13	7.18700589334483e-05	9.70142977291842	188179.926264417	Inf	0.763784339928386	-36	-32	0	-32	0	0	0	0	-4	0	1	-5	-8	-8	-7	-0.443009118541033	-0.451421188630491	0.0794327610195296	-0.868883205456095	-0.316753926701571	0.831578947368421	-0.677100494233937	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.914	-0.779	8:8	149/150	Q:F:Y:I:V:L:T:A:S:G	12.1451538387253	0.919026337424195	119.468044436163	0.101660271548305	5.01153666069207e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	GLU	SRR5817469	SRR5817469	lineage2.2.1	au	Australia	pnca_p.ala46glu	A	E	ALA	AA46	pnca_complex.pdb
104	A46P	L2	PZA	A	A46	46	P	1	XDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	11.322	-2.408	-0.221	2.09234	-4.42	0.65	81	91	-0.822	8	8	0	0	strand	1.76666666666667	8.13	0.0001437401178668	19.411022297013	511754.463552641	Inf	4.73122070382274	0	0	0	0	2	2	0	0	-10	0	-8	-4	-2	0	-3	-0.609929078014184	-0.0571059431524547	0.0762075772696479	-0.753623188405797	0.365168539325843	0.852631578947368	-0.677100494233937	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.914	-0.779	8:8	149/150	Q:F:Y:I:V:L:T:A:S:G	13.1456002256206	0.924803768447002	139.277183326574	0.0943844491369209	0.0001628371344451	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	PRO	TB-17	ERR2851370	lineage2.2.1	lb	Lebanon	pnca_p.ala46pro	A	E	ALA	AA46	pnca_complex.pdb
105	A46T	L4	PZA	A	A46	46	T	0	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	11.322	-1.355	-1.192	-1.34478	-2.283	-0.62	-6	53	-0.822	8	8	0	0	strand	1.76666666666667	8.13	0.0001437401178668	4.85029436501262	4.85029432717733	4.84956447904112	0.0881192621863332	-2	0	0	0	-2	0	-2	0	-9	0	-2	-7	-2	-2	-3	-0.343211752786221	-0.308010335917313	-0.258551872644774	-0.38925831202046	-0.162303664921466	-0.0645161290322581	-0.677100494233937	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.914	-0.779	8:8	149/150	Q:F:Y:I:V:L:T:A:S:G	1.57903938912402	0.264247566376724	1.41439286276584	1.11640791656443	0.191756076673846	122.683760398934	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage4	other_mutations_pyrazinamide	THR	ERR2041741	ERR2041741	lineage4.1.2.1	rs	Serbia	pnca_p.ala46thr	A	E	ALA	AA46	pnca_complex.pdb
106	A46V	L5	PZA	A	A46	46	V	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.322	-2.718	1.017	-2.38862	-1.487	0.44	59	75	-0.822	8	8	0	0	strand	1.76666666666667	8.13	0.0004312203536006	9.712	9.7119892297561	9.70929820233251	7.20065367726982	-2	0	0	0	0	0	0	0	-7	0	1	-8	-2	2	-7	-0.688449848024316	0.864795918367347	-0.459244020610628	-0.253537936913896	0.247191011235955	0.621052631578947	-0.677100494233937	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-0.914	-0.779	8:8	149/150	Q:F:Y:I:V:L:T:A:S:G	2.27336112535402	0.0086576033028962	0.865947753893075	2.62528670480821	1.89450831676925	70.0912742677304	0.987308673731182	0.0095462527031996	2.02016707341074	1.39065774214338	107.435987551185	0.0072877030810549	lineage5	dr_mutations_pyrazinamide	VAL	ERR502508	ERR502508	lineage5	gh	Ghana	pnca_p.ala46val	A	E	ALA	AA46	pnca_complex.pdb
107	T47A	L2	PZA	T	T47	47	A	1	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	7.503	-2.172	-1.71	2.87617	-2.276	-0.77	58	75	-0.925	8	8	0	0	strand	-0.933333333333333	10.3	0.0008624407072013	4.85840707964602	4.85840704522462	4.85767795624558	6.99654790120219	4	0	0	0	0	0	0	2	6	0	4	2	12	6	9	-0.550151975683891	-0.441860465116279	0.10475637205982	-0.388064791133845	-0.201570680628272	0.610526315789474	-0.761943986820428	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.028	-0.872	9:8	149/150	C:K:I:T:S	1.58071061571228	0.0062231325401172	0.577790125858072	2.73578682807148	1.51864513964879	15.5428887748675	0.686493900966672	0.0089768751657447	2.04687481416212	1.29740576993775	18.1865471788576	0.0081667056971109	lineage2	dr_mutations_pyrazinamide	ALA	2564N-sc-2012-10-11T13:40:44Z-1494346,ERR234668	ERR234668	lineage2.2.1	ru	Russia,Samara	pnca_p.thr47ala	A	E	THR	TA47	pnca_complex.pdb
108	T47I	L4	PZA	T	T47	47	I	0	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	7.503	-2.112	-0.155	-1.0395	-1.52	-0.62	72	85	-0.925	8	8	0	0	strand	-0.933333333333333	10.3	0.0003593502946672	1.21225820016821	1.21225918982515	1.212214074125	7.19297638498975e-25	-32	2	0	2	0	0	0	2	-2	0	-3	-1	2	0	4	-0.534954407294833	-0.0400516795865633	-0.199857725140352	-0.259164535379369	-0.162303664921466	0.757894736842105	-0.761943986820428	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.028	-0.872	9:8	149/150	C:K:I:T:S	0.192485717774877	0.863268405978747	1.11770505415734	0.172215126932566	0.061955084955231	8.19905671742341	0.083595130527928	1	0	0.0246053772172713	12.260612593354	0.999999999999323	lineage4	other_mutations_pyrazinamide	ILE	SAMN10961346	SRR8651588	lineage4.4.2	cn	China:Wenzhou	pnca_p.thr47ile	A	E	THR	TA47	pnca_complex.pdb
109	T47N	L3	PZA	T	T47	47	N	0	Pre-XDR	OM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	7.503	-0.208	-1.831	0.956982	-5.182	-1.29	77	85	-0.925	8	8	0	0	strand	-0.933333333333333	10.3	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-26	2	0	2	-4	0	-2	2	0	0	1	-1	-4	-6	2	-0.0526849037487335	-0.47312661498708	0.0348553675361854	-0.883546462063086	-0.337696335078534	0.810526315789474	-0.761943986820428	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.028	-0.872	9:8	149/150	C:K:I:T:S	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987742	NA	4692673.51081218	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	ASN	ERR2510633	ERR2510633	lineage3	pk	Pakistan	pnca_p.thr47asn	A	E	THR	TA47	pnca_complex.pdb
110	T47P	L3	PZA	T	T47	47	P	0	MDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	7.503	-1.892	-1.493	1.93613	-5.056	-1.52	87	91	-0.925	8	8	0	0	strand	-0.933333333333333	10.3	0.0001437401178668	1.2119588062211	4.5970013133425e-05	0	1.01056822389159e-25	4	2	0	2	2	2	0	2	4	0	2	2	8	6	0	-0.479229989868288	-0.38578811369509	0.0705180690418782	-0.862063086104007	-0.397905759162304	0.91578947368421	-0.761943986820428	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.028	-0.872	9:8	149/150	C:K:I:T:S	-9.98752126245062	0.942832958100734	139.277183325398	-0.071709672926946	NA	144471.70631486	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage3	dr_mutations_pyrazinamide	PRO	ERR2510424	ERR2510424	lineage3	pk	Pakistan	pnca_p.thr47pro	A	E	THR	TA47	pnca_complex.pdb
111	K48E	L2	PZA	K	K48	48	E	NA	XDR	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	7.546	-1.91	-1.833	2.3862	-1.921	-1.95	53	75	-0.823	8	8	19	0.0805084745762711	strand	-2.7	6.78	0.000646830530401	6.07150379106992	6.07150374077153	6.07036843596735	6.87801392972404	28	-28	0	-28	2	0	2	0	1	0	0	1	0	0	0	-0.483789260385005	-0.473643410852713	0.0869105981249863	-0.327536231884058	-0.510471204188482	0.557894736842105	-0.677924217462932	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.914	-0.779	8:8	149/150	I:K:R:L:Q:A	1.80360630763137	0.00720618942243	0.671193623356544	2.68716245933892	1.60530087074952	24.5460966015952	0.783296270528047	0.0099794518593429	2.00089331252028	1.30550120941319	30.6177596174465	0.0087262477054536	lineage2	dr_mutations_pyrazinamide	GLU	SAMN02360604	SRR1049046_SRR1049047_SRR1013650_SRR1013651	lineage2.2.2	kr	South Korea	pnca_p.lys48glu	A	E	LYS	KA48	pnca_complex.pdb
112	K48N	L3	PZA	K	K48	48	N	0	Pre-XDR	OM	hydrophobic	hydrophilic	basic	neutral	pos	polar	7.546	-1.168	-2.178	2.2078	-2.869	-1.86	67	80	-0.823	8	8	19	0.0805084745762711	strand	-2.7	6.78	0.0001437401178668	1.2119588062211	4.59700131334259e-05	0	1.01056822389159e-25	46	24	0	24	0	0	0	0	0	0	-2	1	2	0	-2	-0.295845997973658	-0.562790697674419	0.0804128817954676	-0.489173060528559	-0.486910994764398	0.705263157894737	-0.677924217462932	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.914	-0.779	8:8	149/150	I:K:R:L:Q:A	-9.9875212624506	0.942832958100734	139.277183325398	-0.0717096729269463	NA	144471.706314829	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage3	other_mutations_pyrazinamide	ASN	ERR2510272	ERR2510272	lineage3	pk	Pakistan	pnca_p.lys48asn	A	E	LYS	KA48	pnca_complex.pdb
113	K48Q	L4	PZA	K	K48	48	Q	NA	Pre-MDR	OM	hydrophobic	hydrophilic	basic	neutral	pos	polar	7.546	-1.653	-1.537	1.38977	-0.028	-1.89	-61	82	-0.823	8	8	19	0.0805084745762711	strand	-2.7	6.78	NA	NA	NA	NA	NA	28	24	0	24	0	0	2	-2	2	0	1	1	2	2	-4	-0.418693009118541	-0.397157622739018	0.0506184485609598	-0.00477408354646206	-0.494764397905759	-0.655913978494624	-0.677924217462932	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.914	-0.779	8:8	149/150	I:K:R:L:Q:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLN	ERR502532	ERR502532	lineage4.3	gh	Ghana	pnca_p.lys48gln	A	E	LYS	KA48	pnca_complex.pdb
114	K48T	L4	PZA	K	K48	48	T	NA	Pre-XDR	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	7.546	-2.085	-1.92	2.8148	0.126	-1.82	63	80	-0.823	8	8	19	0.0805084745762711	strand	-2.7	6.78	0.0011499209429351	3.77937043282743	3.77937041554348	3.77886521261346	6.25531101162114	58	24	0	24	2	0	2	0	1	0	-1	2	0	4	-2	-0.528115501519757	-0.496124031007752	0.102521143073595	0.106418918918919	-0.476439790575916	0.663157894736842	-0.677924217462932	Destabilising	Destabilising	Destabilising	Stabilising	Destabilising	effect	-0.914	-0.779	8:8	149/150	I:K:R:L:Q:A	1.32955743902485	0.0083975040006128	0.504450465587511	2.6356550934617	1.34958413760038	10.1533105792859	0.577419461128044	0.0115372080052907	1.93789927736761	1.1946979725449	11.4155701668003	0.0123821507399189	lineage4	dr_mutations_pyrazinamide	THR	ERR751528	ERR751528	lineage4.3.3	pe		pnca_p.lys48thr	A	E	LYS	KA48	pnca_complex.pdb
115	D49A	L2	PZA	D	D49	49	A	1	MDR	DM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	3.452	-3.348	-0.451	-2.06724	-1.72	-1.56	73	85	-1.188	9	9	7	0.0362694300518134	strand	-1.53333333333333	7.89	0.0004312203536006	58.3312262958281	1393450.9402217	Inf	23.547488635408	20	28	0	28	0	0	0	0	-5	0	0	-5	0	2	3	-0.848024316109423	-0.116537467700258	-0.397454433592248	-0.293265132139812	-0.408376963350785	0.768421052631579	-0.978583196046129	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	T:D	14.1472939191285	0.915018435774987	132.576060676241	0.106710773023171	4.17896182049081	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage2	dr_mutations_pyrazinamide	ALA	ERR2517097	ERR2517097	lineage2.2.1.2	gb	UK	pnca_p.asp49ala	A	E	ASP	DA49	pnca_complex.pdb
116	D49E	L4	PZA	D	D49	49	E	NA	MDR	DM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	3.452	0.251	-0.472	-0.696543	-2.35	-0.28	75	85	-1.188	9	9	7	0.0362694300518134	strand	-1.53333333333333	7.89	0.0002156101768003	9.70466975178797	9.70465898653052	9.70194990589383	2.29168245016684	-22	0	0	0	0	0	0	0	-5	0	-1	-6	-10	-6	-8	0.112556053811659	-0.121963824289406	-0.133919672383296	-0.400682011935209	-0.0732984293193717	0.789473684210526	-0.978583196046129	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	T:D	2.27260607808817	0.0634455902260897	1.22442760158496	1.8560559033024	0.929172866365932	208.881603004782	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage4	dr_mutations_pyrazinamide	GLU	ERR1555056	ERR1555056	lineage4.3.3			pnca_p.asp49glu	A	E	ASP	DA49	pnca_complex.pdb
117	D49G	L4	PZA	D	D49	49	G	1	XDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	3.452	-3.463	-1.157	0.460983	-2.415	-1.08	83	91	-1.188	9	9	7	0.0362694300518134	strand	-1.53333333333333	7.89	0.0005030904125341	29.1630847029077	29.1630732435189	29.1293325978315	18.6717493808583	28	30	0	30	0	0	0	0	0	0	0	-1	6	9	19	-0.877152988855116	-0.298966408268734	0.0167900042978169	-0.411764705882353	-0.282722513089005	0.873684210526316	-0.978583196046129	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	T:D	3.37290329402599	0.0017890770828182	1.07995768762032	3.12318096596744	4.97876379054023	550.894323518622	1.46483345924118	0.0001485295621003	3.828187099348	3.53443753556636	1331.56873199938	1.55265977742841e-05	lineage4	dr_mutations_pyrazinamide	GLY	ERR2516206	ERR2516206	lineage4.3.3	be	Belgium	pnca_p.asp49gly	A	E	ASP	DA49	pnca_complex.pdb
118	D49N	L4	PZA	D	D49	49	N	NA	MDR	DM	hydrophilic	hydrophilic	acidic	neutral	neg	polar	3.452	-1.932	-1.684	-0.334669	-2.857	-1.45	76	85	-1.188	9	9	7	0.0362694300518134	strand	-1.53333333333333	7.89	0.0005749604714675	77.8405735976381	1394626.35223403	Inf	33.1802749271012	0	32	0	32	-2	0	0	-2	-6	0	0	-6	-2	0	0	-0.48936170212766	-0.435142118863049	-0.064344574329001	-0.487127024722933	-0.379581151832461	0.8	-0.978583196046129	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	T:D	14.148137089499	0.901927875940367	114.814237034002	0.123226330244298	82.9586021424457	NA	1.89120602753485	7.2326389241213e-07	6.1407032156959	8.29603358299821	Inf	8.39983046099388e-09	lineage4	dr_mutations_pyrazinamide	ASN	ERR775328	ERR775328	lineage4.3.3	pe		pnca_p.asp49asn	A	E	ASP	DA49	pnca_complex.pdb
119	D49Y	L4	PZA	D	D49	49	Y	1	MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	3.452	-1.855	-0.739	-2.66914	-0.988	-1.64	85	91	-1.188	9	9	7	0.0362694300518134	strand	-1.53333333333333	7.89	7.18700589334483e-05	9.70142977291842	188179.926264654	Inf	0.763784339928386	-42	20	0	20	-2	0	0	-4	-14	0	-4	-10	-14	-12	-12	-0.469858156028369	-0.190956072351421	-0.513177728216565	-0.16845694799659	-0.429319371727749	0.894736842105263	-0.978583196046129	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	T:D	12.1451538387266	0.919026337424238	119.468044436239	0.10166027154825	5.0115366606202e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	TYR	ERR2510658	ERR2510658	lineage4.3.3	it	Italy	pnca_p.asp49tyr	A	E	ASP	DA49	pnca_complex.pdb
120	H51D	L4	PZA	H	H51	51	D	1	Pre-MDR	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	5.661	-1.821	-2.198	4.34022	-1.814	-1.67	82	91	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	0.0030185424752048	414.608472400514	10454944.8180635	Inf	198.436695636911	16	-28	0	-28	2	0	2	0	4	0	2	2	2	4	4	-0.461246200607903	-0.567958656330749	0.158080259908653	-0.309292412617221	-0.43717277486911	0.863157894736842	-0.979406919275124	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	16.1625856127882	0.905545449550291	136.210261834263	0.118659089228199	26043.5577250657	1.23987518604392e+26	2.61763817252677	4.4914140361843e-33	32.347616908121	53.6831618314533	Inf	4.58127580776551e-45	lineage4	dr_mutations_pyrazinamide	ASP	ERR552878	ERR552878	lineage4.4.1.1	sz	Swaziland	pnca_p.his51asp	A	B	HIS	HA51	pnca_complex.pdb
121	H51N	L4	PZA	H	H51	51	N	NA	MDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	5.661	-1.063	-2.215	1.03208	-2.201	-1.83	70	85	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	NA	NA	NA	NA	NA	14	0	0	0	0	0	0	0	0	0	0	0	-4	2	0	-0.269250253292806	-0.57235142118863	0.0375906001646282	-0.375277067348679	-0.479057591623037	0.736842105263158	-0.979406919275124	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	ASN	ERR2516444	ERR2516444	lineage4.1.2.1	nl	Netherlands	pnca_p.his51asn	A	B	HIS	HA51	pnca_complex.pdb
122	H51P	L2	PZA	H	H51	51	P	1	Pre-XDR	DM	hydrophobic	hydrophilic	basic	non-polar	pos	non-polar	5.661	-2.624	-1.762	2.82591	-1.529	-0.98	82	91	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	0.0002156101768003	2.42493692178301	2.42493957425811	2.42473736947533	4.31445715363782e-25	28	0	0	0	4	2	2	0	0	0	-2	2	4	6	2	-0.664640324214792	-0.455297157622739	0.10292579345712	-0.260699062233589	-0.256544502617801	0.863157894736842	-0.979406919275124	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	0.885806606247285	0.469406216824825	1.22442753211922	0.723445514749364	0.112662937620387	25.3270547471172	0.384700446081433	0.430258985168156	0.366270051463612	0.0410854444106247	46.5881472867033	0.999999999999476	lineage2	dr_mutations_pyrazinamide	PRO	0111X-sc-2012-10-11T13:37:09Z-1494123,ERR234637	ERR234637	lineage2.2.1	ru	Russia,Samara	pnca_p.his51pro	A	B	HIS	HA51	pnca_complex.pdb
123	H51Q	L2	PZA	H	H51	51	Q	NA	Pre-XDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	5.661	-1.42	-1.714	-1.10489	-1.18	-1.75	65	80	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	0.0004312203536006	24.2923336141533	24.29233342416	24.2833096922897	14.1977442691074	0	0	0	0	-4	0	-2	-2	2	0	2	0	-6	-2	-4	-0.3596757852077	-0.442894056847545	-0.212429823886795	-0.201193520886615	-0.458115183246073	0.684210526315789	-0.979406919275124	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	3.19016080361872	0.0035954281300595	1.09565968954934	2.91163472932995	3.91551977432275	465.531063220863	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage2	dr_mutations_pyrazinamide	GLN	SRS935961,SAMN03653227	SRR2024921	lineage2.2.1	cn	China	pnca_p.his51gln	A	B	HIS	HA51	pnca_complex.pdb
124	H51R	L4	PZA	H	H51	51	R	NA	MDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	5.661	-0.862	-1.766	2.7037	-1.252	-1.48	74	85	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	0.001293661060802	24.3908629441624	24.3908627908724	24.3817411979725	51.2029377973759	-32	-2	0	-2	0	0	2	-2	-10	0	-2	-9	0	2	-2	-0.218338399189463	-0.456330749354005	0.0984746392383394	-0.213469735720375	-0.387434554973822	0.778947368421053	-0.979406919275124	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	3.19420858638167	4.47975895465189e-07	0.632848408547862	5.04735185115046	8.04148719104289	105.368248724156	1.38722716584297	1.45797891284227e-09	8.83624875729546	6.89001106760784	131.337835841673	8.32938806400108e-13	lineage4	dr_mutations_pyrazinamide	ARG	ERR779915	ERR779915	lineage4.3.3	pe		pnca_p.his51arg	A	B	HIS	HA51	pnca_complex.pdb
125	H51Y	L4	PZA	H	H51	51	Y	NA	MDR	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	5.661	-2.046	0.122	-1.10156	-0.891	0.41	60	80	-1.189	9	9	1	0.0044642857142857	strand	1.36666666666667	6.97	0.0002156101768003	9.70466975178797	9.70465898653051	9.70194990589383	2.29168245016684	-18	-6	0	-6	2	0	2	0	-4	0	2	-6	-2	4	-4	-0.518237082066869	0.103741496598639	-0.21178958701838	-0.151918158567775	0.230337078651685	0.631578947368421	-0.979406919275124	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-1.221	-1.178	9:9	149/150	H:Y	2.27260607808817	0.0634455902260897	1.22442760158496	1.8560559033024	0.929172866365331	208.881603005168	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage4	dr_mutations_pyrazinamide	TYR	ERR2707212	ERR2707212	lineage4.3.3	cd	Democratic Republic of Congo	pnca_p.his51tyr	A	B	HIS	HA51	pnca_complex.pdb
126	I52T	L4	PZA	I	I52	52	T	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	12.353	0.529	-1.196	0.950846	-1.262	-1.33	26	63	-0.518	7	7	82	0.416243654822335	loop	-0.733333333333333	4.09	NA	NA	NA	NA	NA	6	0	0	0	-2	0	0	-2	-1	0	-3	0	0	0	-2	0.237219730941704	-0.309043927648579	0.0346318810597397	-0.215174765558397	-0.348167539267016	0.273684210526316	-0.42668863261944	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	149/150	N:L:V:I:S:T:P:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	THR	ERR1465772	ERR1465772	lineage4.1.2.1	ch	Switzerland	pnca_p.ile52thr	A	S	ILE	IA52	pnca_complex.pdb
127	D53E	L4	PZA	D	D53	53	E	0	Sensitive	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	13.16	-0.123	-0.145	0.906346	-0.467	0.14	-39	66	0.498	3	3	103	0.533678756476684	loop	-0.2	3.83	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-6	2	0	2	0	0	0	0	-1	0	-1	0	-2	-2	0	-0.0311550151975683	-0.0374677002583979	0.0330110941950335	-0.0796248934356351	0.0786516853932584	-0.419354838709677	0.159615384615385	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	neutral	0.225	0.59	4:3	148/150	D:P:S:G:A:I:R:L:N:V:Q:E:H	-8.9874087814964	0.940032862253633	119.468044435491	-0.0752285585987752	NA	4692673.51081297	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	GLU	SAMN03648619,SRR2101372	SRR2101372	lineage4.3.4.2.1			pnca_p.asp53glu	A	-	ASP	DA53	pnca_complex.pdb
128	P54A	L2	PZA	P	P54	54	A	NA	MDR	OM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.765	-1.785	-1.416	2.25796	-1.236	-1.58	25	63	-0.129	5	5	8	0.050314465408805	loop	-1.83333333333333	4.91	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	0	0	0	0	0	0	0	-1	0	-1	0	0	0	1	-0.452127659574468	-0.365891472868217	0.0822398181804938	-0.21074168797954	-0.413612565445026	0.263157894736842	-0.106260296540362	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	148/150	Q:E:H:K:V:N:P:A:G:S:D	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987755	NA	4692673.51081266	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	ALA	SRR671771,BGI_GZ10115	SRR671771	lineage2.2.1	cn	China	pnca_p.pro54ala	A	-	PRO	PA54	pnca_complex.pdb
129	P54L	L4	PZA	P	P54	54	L	1	Pre-XDR	DM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.765	-1.77	-0.0969999999999999	3.46567	-0.95	-0.4	33	66	-0.129	5	5	8	0.050314465408805	loop	-1.83333333333333	4.91	0.0023717119448037	22.0580357142857	22.0580329576526	22.0491621136906	93.2270586345296	-16	0	0	0	0	0	0	0	-10	0	-6	-4	-2	-2	-2	-0.448328267477204	-0.0250645994832041	0.126227245245085	-0.161977834612106	-0.104712041884817	0.347368421052632	-0.106260296540362	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	148/150	Q:E:H:K:V:N:P:A:G:S:D	3.09367684201887	7.52888191547387e-12	0.451813758844646	6.84723911447463	9.73717599077908	59.1593944160241	1.34356683555525	6.54726814958295e-16	15.1839398717709	8.90776823243485	65.3653818513773	4.6626285706706e-22	lineage4	dr_mutations_pyrazinamide	LEU	ERR1873531	ERR1873531	lineage4.1.1.3	za	South Africa	pnca_p.pro54leu	A	-	PRO	PA54	pnca_complex.pdb
130	P54Q	L2	PZA	P	P54	54	Q	1	XDR	DM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	9.765	-0.084	-1.251	9.94224	-1.258	-1.64	8	53	-0.129	5	5	8	0.050314465408805	loop	-1.83333333333333	4.91	0.0003593502946672	19.4256842105263	19.4256680571768	19.4169790983636	9.87561363723508	-16	0	0	0	-3	0	-1	-2	-8	0	-8	-4	-10	-8	-6	-0.0212765957446808	-0.323255813953488	0.362118022421492	-0.214492753623188	-0.429319371727749	0.0842105263157895	-0.106260296540362	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	148/150	Q:E:H:K:V:N:P:A:G:S:D	2.96659528728018	0.0079500503166053	1.11770528264822	2.65418382943607	2.872152136516	380.097158975598	1.28837632444196	0.0036816889015929	2.43395291165754	1.92061868691892	951.403114304703	0.0016748373055023	lineage2	dr_mutations_pyrazinamide	GLN	ERR158587,2129Q-sc-2012-04-10T09:16:19Z-1397799	ERR158587	lineage2.2.1	ru	Russia,Samara	pnca_p.pro54gln	A	-	PRO	PA54	pnca_complex.pdb
131	P54R	L4	PZA	P	P54	54	R	1	Pre-XDR	OM	hydrophilic	hydrophilic	non-polar	basic	non-polar	pos	9.765	-0.957	-0.884	7.06229	-1.269	-0.35	33	66	-0.129	5	5	8	0.050314465408805	loop	-1.83333333333333	4.91	0.0001437401178668	19.411022297013	511754.463552342	Inf	4.73122070382274	-40	-24	0	-24	-4	0	0	-4	-19	0	-8	-16	-12	-12	-12	-0.242401215805471	-0.228423772609819	0.257223974533614	-0.216368286445013	-0.0916230366492146	0.347368421052632	-0.106260296540362	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	148/150	Q:E:H:K:V:N:P:A:G:S:D	13.14560022562	0.924803768446982	139.27718332653	0.0943844491369463	0.0001628371344461	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	other_mutations_pyrazinamide	ARG	SAMN10961390	SRR8651581	lineage4.5	cn	China:Wenzhou	pnca_p.pro54arg	A	-	PRO	PA54	pnca_complex.pdb
132	P54S	L2	PZA	P	P54	54	S	NA	Pre-XDR	OM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	9.765	-0.307	-1.837	1.58683	-1.286	-1.76	26	63	-0.129	5	5	8	0.050314465408805	loop	-1.83333333333333	4.91	0.0002156101768003	9.70466975178797	9.70465898653047	9.70194990589383	2.29168245016684	0	-4	0	-4	-6	0	2	-8	-7	0	-3	-2	-6	-6	-4	-0.0777608915906788	-0.474677002583979	0.0577958027083531	-0.21926683716965	-0.460732984293194	0.273684210526316	-0.106260296540362	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	148/150	Q:E:H:K:V:N:P:A:G:S:D	2.27260607808816	0.0634455902260904	1.22442760158496	1.8560559033024	0.929172866366028	208.88160300516	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	other_mutations_pyrazinamide	SER	ERR1023297	ERR1023297	lineage2.2.1			pnca_p.pro54ser	A	-	PRO	PA54	pnca_complex.pdb
133	G55S	L4	PZA	G	G55	55	S	NA	Sensitive	OM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	12.27	0.559	-0.912	0.441313	-1.035	0.06	5	53	-1.036	9	9	36	0.346153846153846	helix	-1.83333333333333	3.54	NA	NA	NA	NA	NA	0	0	0	0	-2	0	-2	0	-2	0	-4	0	0	-3	-7	0.25067264573991	-0.235658914728682	0.01607358008144	-0.176470588235294	0.0337078651685393	0.0526315789473684	-0.85337726523888	Stabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.124	-0.993	9:9	148/150	T:H:S:A:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	SER	ERR2229463	ERR2229463	lineage4.8	dk		pnca_p.gly55ser	A	G	GLY	GA55	pnca_complex.pdb
134	D56E	L2	PZA	D	D56	56	E	NA	Sensitive	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	11.133	-0.01	0.434	-0.915918	-0.032	0.09	-78	87	2.366	1	1	148	0.766839378238342	helix	-2.36666666666667	3.47	NA	NA	NA	NA	NA	-8	-4	0	-4	-1	0	-1	0	0	0	0	-1	0	0	0	-0.0025329280648429	0.369047619047619	-0.176097439052526	-0.00545609548167093	0.0505617977528089	-0.838709677419355	0.758333333333333	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	neutral	1.183	3.125	1:1	148/150	D:P:S:A:G:T:K:C:R:I:L:V:E:Y	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLU	ERR502510	ERR502510	lineage2	gh	Ghana	pnca_p.asp56glu	A	G	ASP	DA56	pnca_complex.pdb
135	H57D	LBOV	PZA	H	H57	57	D	NA	Other	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	4.561	-1.283	-1.854	1.83315	-1.011	-1.24	75	85	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	0.0073307460112117	166.910526315789	166.909698569351	166.066117054134	457.827712322676	12	-52	0	-52	2	0	0	2	0	0	0	2	-2	0	0	-0.324974670719352	-0.47906976744186	0.066767313281711	-0.172378516624041	-0.324607329842932	0.789473684210526	-0.969522240527183	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	148/150	H:S	5.11745293904149	2.3513585122013e-18	0.585586418334624	8.73902259139692	62.7783116073746	679.19417378895	2.2224837265936	2.07661319929242e-72	71.6826443898669	55.3332327668776	812.938834180501	1.42764749548143e-101	lineageBOV	dr_mutations_pyrazinamide	ASP	ERR3275354	ERR3275354	lineageBOV	nl	Netherlands	pnca_p.his57asp	A	G	HIS	HA57	pnca_complex.pdb
136	H57L	L4	PZA	H	H57	57	L	NA	MDR	DM	hydrophobic	hydrophobic	basic	non-polar	pos	non-polar	4.561	-1.924	-0.06	-1.11406	-0.541	-0.64	68	80	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	NA	NA	NA	NA	NA	2	0	0	0	2	0	0	2	0	0	-2	1	0	-2	0	-0.487335359675785	-0.0155038759689922	-0.214192878566485	-0.0922421142369992	-0.167539267015707	0.715789473684211	-0.969522240527183	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	148/150	H:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	LEU	ERR751574	ERR751574	lineage4.3.4.2	pe		pnca_p.his57leu	A	G	HIS	HA57	pnca_complex.pdb
137	H57P	L4	PZA	H	H57	57	P	1	Pre-XDR	DM	hydrophobic	hydrophilic	basic	non-polar	pos	non-polar	4.561	-2.115	-1.226	0.15002	-0.75	-0.33	78	85	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	0.0002874802357337	38.8547368421053	1392277.50785055	Inf	13.9909822352959	22	0	0	0	4	2	0	2	3	4	-3	4	2	0	4	-0.535714285714286	-0.316795865633075	0.00546405495378026	-0.127877237851662	-0.0863874345549738	0.821052631578947	-0.969522240527183	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	148/150	H:S	14.1464514590952	0.930573112990219	162.371849599067	0.0871237932808301	0.0113995443355766	NA	1.58944397188817	0.0008528275207519	3.06913879340442	3.20302487654751	Inf	0.0001836895199759	lineage4	dr_mutations_pyrazinamide	PRO	ERR2510509	ERR2510509	lineage4.1.2.1	pk	Pakistan	pnca_p.his57pro	A	G	HIS	HA57	pnca_complex.pdb
138	H57Q	L4	PZA	H	H57	57	Q	NA	MDR	OM	hydrophobic	hydrophilic	basic	neutral	pos	polar	4.561	-0.949	-1.29	0.84932	-0.454	-0.3	63	80	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	NA	NA	NA	NA	NA	-2	0	0	0	1	0	-1	2	-2	0	-2	2	0	-2	-2	-0.240374873353597	-0.333333333333333	0.0309340831445451	-0.0774083546462063	-0.0785340314136125	0.663157894736842	-0.969522240527183	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	148/150	H:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLN	ERR2707036	ERR2707036	lineage4.3.4.1	cd	Democratic Republic of Congo	pnca_p.his57gln	A	G	HIS	HA57	pnca_complex.pdb
139	H57R	L4	PZA	H	H57	57	R	NA	MDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	4.561	-0.281	-1.171	1.25273	-0.843	-0.28	76	85	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	0.0018686215322696	254.917127071823	3820001.12562827	Inf	120.509921037495	-20	-28	0	-28	2	0	0	2	0	0	-2	3	0	-2	0	-0.0711752786220871	-0.302583979328165	0.0456271534611995	-0.143734015345269	-0.0732984293193717	0.8	-0.969522240527183	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	148/150	H:S	15.1557612752498	0.885234541058947	105.0027715034	0.144336773765625	15316.7789338948	20462689243807308	2.40639901531589	1.01650823124183e-20	19.9928891002838	32.1173872780919	Inf	4.89215110948636e-28	lineage4	dr_mutations_pyrazinamide	ARG	SRR671861,BGI_GuangZ0016	SRR671861	lineage4.4.2	cn	China	pnca_p.his57arg	A	G	HIS	HA57	pnca_complex.pdb
140	H57Y	L2	PZA	H	H57	57	Y	NA	Pre-XDR	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	4.561	-1.155	0.405	-0.147464	-0.488	0.9	63	80	-1.177	9	9	16	0.0714285714285714	helix	-1.3	5.63	0.0002156101768003	29.1287878787879	511969.848428296	Inf	9.2872640727157	-10	-6	0	-6	0	0	0	0	-4	0	-2	0	0	0	0	-0.292553191489362	0.344387755102041	-0.028351918787972	-0.0832054560954817	0.50561797752809	0.663157894736842	-0.969522240527183	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-1.221	-1.152	9:9	148/150	H:S	13.1460210124991	0.907969111549563	113.719344731871	0.115600569485296	0.183926936278874	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage2	dr_mutations_pyrazinamide	TYR	SRR6280334	SRR6280334	lineage2.2.1.1	cn	China	pnca_p.his57tyr	A	G	HIS	HA57	pnca_complex.pdb
141	F58L	L2	PZA	F	F58	58	L	0	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	8.072	-2.337	-1.639	0.155134	-0.746	-1.43	74	85	-1.108	9	9	43	0.179166666666667	strand	-0.4	4.41	0.000646830530401	6.07150379106992	6.0715037407715	6.07036843596735	6.87801392972404	10	2	0	2	0	0	0	0	-3	0	-3	1	2	6	0	-0.591945288753799	-0.423514211886305	0.0056503179656029	-0.127195225916454	-0.37434554973822	0.778947368421053	-0.912685337726524	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	148/150	W:I:F	1.80360630763137	0.0072061894224301	0.671193623356544	2.68716245933892	1.60530087074952	24.5460966015953	0.783296270528047	0.0099794518593429	2.00089331252028	1.30550120941319	30.6177596174465	0.0087262477054536	lineage2	dr_mutations_pyrazinamide	LEU	ERR133844,0311P-sc-2012-02-09T18:55:55Z-1355177	ERR133844	lineage2.2.1	ru	Russia,Samara	pnca_p.phe58leu	A	B	PHE	FA58	pnca_complex.pdb
142	F58S	L2	PZA	F	F58	58	S	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	8.072	-0.806	-2.784	2.9435	-1.022	-3	78	85	-1.108	9	9	43	0.179166666666667	strand	-0.4	4.41	0.0001437401178668	4.85029436501262	4.85029432717734	4.84956447904112	0.0881192621863332	28	2	0	2	0	0	0	-2	0	0	0	2	6	10	2	-0.204154002026342	-0.71937984496124	0.107208677219385	-0.174254049445865	-0.785340314136126	0.821052631578947	-0.912685337726524	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	148/150	W:I:F	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673649	122.683760399218	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	dr_mutations_pyrazinamide	SER	SRR5065526	SRR5065526	lineage2.2.1	vn	Vietnam	pnca_p.phe58ser	A	B	PHE	FA58	pnca_complex.pdb
143	F58V	L5	PZA	F	F58	58	V	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	8.072	-2.319	-1.927	1.56433	-1.103	-1.7	71	85	-1.108	9	9	43	0.179166666666667	strand	-0.4	4.41	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	28	2	0	2	0	0	2	-2	-2	0	-2	2	4	8	-2	-0.587386018237082	-0.497932816537468	0.0569763037318162	-0.188064791133845	-0.445026178010471	0.747368421052632	-0.912685337726524	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	148/150	W:I:F	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987755	NA	4692673.51081266	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage5	other_mutations_pyrazinamide	VAL	ERR2707138	ERR2707138	lineage5	cd	Democratic Republic of Congo	pnca_p.phe58val	A	B	PHE	FA58	pnca_complex.pdb
144	S59F	L4	PZA	S	S59	59	F	NA	MDR	DM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	11.829	-0.598	-0.905	0.176045	-1.083	-0.74	56	75	-0.671	7	7	38	0.245161290322581	loop	0.533333333333333	3.86	7.18700589334483e-05	9.70142977291842	188179.926264404	Inf	0.763784339928386	-14	-2	0	-2	2	0	2	0	-3	0	-1	-2	-2	-4	-2	-0.151469098277609	-0.233850129198966	0.00641194210330786	-0.184654731457801	-0.193717277486911	0.589473684210526	-0.552718286655684	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	148/150	T:G:A:S:Q:H:E:K:R:L:V	12.1451538387252	0.919026337424193	119.468044436159	0.101660271548307	5.01153666069521e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	PHE	ERR2707143	ERR2707143	lineage4.3.3	cd	Democratic Republic of Congo	pnca_p.ser59phe	A	-	SER	SA59	pnca_complex.pdb
145	S59P	L4	PZA	S	S59	59	P	NA	MDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	11.829	-1.097	-0.594	3.6058	-1.13	-0.16	62	80	-0.671	7	7	38	0.245161290322581	loop	0.533333333333333	3.86	0.0004312203536006	58.3312262958281	1393450.94022764	Inf	23.547488635408	0	0	0	0	4	2	2	0	-6	0	-4	-2	-2	-4	-6	-0.277862208713272	-0.153488372093023	0.131331084870956	-0.192668371696505	-0.0418848167539267	0.652631578947368	-0.552718286655684	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	148/150	T:G:A:S:Q:H:E:K:R:L:V	14.1472939191327	0.915018435775139	132.57606067652	0.106710773022978	4.17896182039693	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage4	dr_mutations_pyrazinamide	PRO	SAMN02403622	SRR1175510_SRR1175516	lineage4.8	za	South Africa	pnca_p.ser59pro	A	-	SER	SA59	pnca_complex.pdb
146	S59Y	L2	PZA	S	S59	59	Y	1	Pre-XDR	OM	hydrophilic	hydrophobic	neutral	neutral	polar	polar	11.829	-0.553	-0.542	0.447426	-0.986	-0.66	58	75	-0.671	7	7	38	0.245161290322581	loop	0.533333333333333	3.86	7.18700589334483e-05	9.70142977291842	188179.926264634	Inf	0.763784339928386	-22	-2	0	-2	2	0	2	0	-1	0	-1	0	0	-2	-2	-0.140070921985815	-0.140051679586563	0.0162962288478209	-0.168115942028985	-0.172774869109948	0.610526315789474	-0.552718286655684	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.615	8:7	148/150	T:G:A:S:Q:H:E:K:R:L:V	12.1451538387264	0.919026337424234	119.468044436232	0.101660271548256	5.0115366606273e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	TYR	SAMN10961392	SRR8651662	lineage2.2.1	cn	China:Wenzhou	pnca_p.ser59tyr	A	-	SER	SA59	pnca_complex.pdb
147	G60D	L4	PZA	G	G60	60	D	0	Sensitive	OM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	14.904	0.52	-0.161	-2.08267	0.187	-0.13	-38	66	0.72	2	2	80	0.769230769230769	loop	-0.633333333333333	3.26	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	-8	0	-8	-4	0	0	-4	-1	0	-1	0	0	-1	-7	0.233183856502242	-0.0416020671834625	-0.400421056679228	0.157939189189189	-0.0340314136125654	-0.408602150537634	0.230769230769231	Stabilising	Destabilising	Stabilising	Stabilising	Destabilising	neutral	0.389	0.842	4:2	148/150	H:E:Q:N:L:V:K:I:T:A:S:G:P:D	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987745	NA	4692673.51081424	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	ASP	ERS2286463	ERR2503536	lineage4.8	fi	Finland	pnca_p.gly60asp	A	S	GLY	GA60	pnca_complex.pdb
148	T61P	L4	PZA	T	T61	61	P	NA	MDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	14.618	-0.472	-0.204	1.58134	-0.158	0.02	-13	57	1.323	1	1	130	0.755813953488372	loop	-0.9	3.48	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	0	0	0	0	0	0	0	0	2	-2	0	0	0	-2	-0.119554204660588	-0.0527131782945736	0.0575958449580781	-0.0269394714407502	0.0112359550561797	-0.139784946236559	0.424038461538462	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	neutral	0.842	1.712	2:1	124/150	R:K:N:L:V:Q:H:E:D:P:T:A:G:S	-8.98740878149644	0.940032862253633	119.468044435491	-0.0752285585987752	NA	4692673.51081345	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	dr_mutations_pyrazinamide	PRO	SAMN02403629	SRR1175497_SRR1175498_SRR1175480_SRR1175526	lineage4.1.2.1	za	South Africa	pnca_p.thr61pro	A	S	THR	TA61	pnca_complex.pdb
149	T61R	L1	PZA	T	T61	61	R	NA	Pre-MDR	OM	hydrophobic	hydrophilic	neutral	basic	polar	pos	14.618	-0.01	0.174	-0.362941	-0.011	-0.02	-37	66	1.323	1	1	130	0.755813953488372	loop	-0.9	3.48	NA	NA	NA	NA	NA	-8	-4	0	-4	0	0	0	0	-1	0	-1	-1	0	0	0	-0.0025329280648429	0.147959183673469	-0.0697802430208413	-0.00187553282182438	-0.0052356020942408	-0.397849462365591	0.424038461538462	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	neutral	0.842	1.712	2:1	124/150	R:K:N:L:V:Q:H:E:D:P:T:A:G:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	ARG	ERR702404	ERR702404	lineage1	gh	Ghana	pnca_p.thr61arg	A	S	THR	TA61	pnca_complex.pdb
150	P62L	L4	PZA	P	P62	62	L	1	MDR	DM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.121	-0.276	-0.504	1.77658	-1.127	-0.59	54	75	-0.599	7	7	34	0.213836477987421	loop	-1.93333333333333	3.99	0.0007905706482679	48.6872097931617	48.6872048041474	48.6746878162623	37.2619168572472	0	-2	0	-2	0	0	0	0	-1	-2	-7	1	2	4	2	-0.0699088145896656	-0.130232558139535	0.0647069107438137	-0.192156862745098	-0.154450261780105	0.568421052631579	-0.49341021416804	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.485	8:7	125/150	I:V:P:A:E	3.88541626053988	0.0002118075877292	1.0488367141091	3.70450062271154	9.31977387255173	893.783372292271	1.68741488635211	1.95392414212297e-07	6.70909230105579	6.91781679568266	2088.84994220519	1.03281930722422e-09	lineage4	dr_mutations_pyrazinamide	LEU	ERR1034715	ERR1034715	lineage4.1.2.1	pe	Peru	pnca_p.pro62leu	A	-	PRO	PA62	pnca_complex.pdb
151	P62Q	L4	PZA	P	P62	62	Q	NA	Pre-MDR	DM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	11.121	0.708	-0.763	1.97038	-0.98	-0.28	50	75	-0.599	7	7	34	0.213836477987421	loop	-1.93333333333333	3.99	NA	NA	NA	NA	NA	-6	0	0	0	-3	0	-1	-2	-7	-2	-3	-3	0	0	0	0.317488789237668	-0.197157622739018	0.071765528595051	-0.167092924126172	-0.0732984293193717	0.526315789473684	-0.49341021416804	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.485	8:7	125/150	I:V:P:A:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	GLN	SRR5065206	SRR5065206	lineage4.4.1.2	vn	Vietnam	pnca_p.pro62gln	A	-	PRO	PA62	pnca_complex.pdb
152	P62R	L4	PZA	P	P62	62	R	NA	XDR	DM	hydrophilic	hydrophilic	non-polar	basic	non-polar	pos	11.121	0.023	-0.186	2.51854	-1.078	-0.18	61	80	-0.599	7	7	34	0.213836477987421	loop	-1.93333333333333	3.99	NA	NA	NA	NA	NA	-20	-14	0	-14	0	0	0	0	-7	-2	-1	-5	-2	0	0	0.0103139013452914	-0.0480620155038759	0.0917307089940923	-0.183802216538789	-0.0471204188481675	0.642105263157895	-0.49341021416804	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.485	8:7	125/150	I:V:P:A:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	ARG	ERR775322	ERR775322	lineage4.1.1	pe		pnca_p.pro62arg	A	-	PRO	PA62	pnca_complex.pdb
153	P62S	L2	PZA	P	P62	62	S	0	XDR	DM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	11.121	0.534	-1.342	1.54135	-1.161	-1.23	50	75	-0.599	7	7	34	0.213836477987421	loop	-1.93333333333333	3.99	0.0003593502946672	1.21225820016821	1.21225918982515	1.212214074125	7.19297638498975e-25	0	0	0	0	-2	0	-2	0	-3	-2	-1	0	-4	-4	0	0.239461883408072	-0.346770025839793	0.0561393221104466	-0.197953964194373	-0.321989528795811	0.526315789473684	-0.49341021416804	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.485	8:7	125/150	I:V:P:A:E	0.192485717774876	0.863268405978748	1.11770505415734	0.172215126932565	0.0619550849552706	8.19905671742343	0.083595130527928	1	0	0.0246053772172713	12.260612593354	0.999999999999323	lineage2	dr_mutations_pyrazinamide	SER	ERR2517004	ERR2517004	lineage2.2.1	gb	UK	pnca_p.pro62ser	A	-	PRO	PA62	pnca_complex.pdb
154	P62T	L3	PZA	P	P62	62	T	1	Pre-XDR	OM	hydrophilic	hydrophobic	non-polar	neutral	non-polar	polar	11.121	0.522	-1.056	2.20908	-1.242	-0.58	55	75	-0.599	7	7	34	0.213836477987421	loop	-1.93333333333333	3.99	0.0002156101768003	29.1287878787879	511969.848429208	Inf	9.2872640727157	0	0	0	0	0	0	0	0	-9	-2	-5	-2	-4	-4	0	0.234080717488789	-0.272868217054264	0.0804595021816884	-0.211764705882353	-0.151832460732984	0.578947368421053	-0.49341021416804	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.779	-0.485	8:7	125/150	I:V:P:A:E	13.1460210125009	0.907969111549634	113.719344731975	0.115600569485207	0.183926936276724	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage3	other_mutations_pyrazinamide	THR	ERR2510418	ERR2510418	lineage3	pk	Pakistan	pnca_p.pro62thr	A	-	PRO	PA62	pnca_complex.pdb
155	D63A	L2	PZA	D	D63	63	A	1	MDR	DM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	10.737	-0.769	-0.714	4.4472	-0.704	0.12	40	71	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	0.0009343107661348	26.7869510135135	26.7869506340703	26.7708032978517	37.2159659891988	2	12	0	12	4	0	4	6	3	0	3	0	0	2	1	-0.194782168186423	-0.184496124031008	0.16197670437576	-0.12003410059676	0.0674157303370786	0.421052631578947	-0.610378912685338	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	3.28791485229524	1.90630753051068e-05	0.768996545261043	4.275591187707	7.18677177005967	173.199151011581	1.42792328347145	1.9896368453757e-07	6.70122618511891	5.83859157504838	247.443798048456	1.05744462378296e-09	lineage2	dr_mutations_pyrazinamide	ALA	ERR1034857	ERR1034857	lineage2.2.1	pt	Portugal	pnca_p.asp63ala	A	-	ASP	DA63	pnca_complex.pdb
156	D63E	L2	PZA	D	D63	63	E	NA	XDR	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	10.737	-0.0139999999999999	-0.219	3.8992	-0.86	0.02	32	66	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	NA	NA	NA	NA	NA	-4	6	0	6	4	0	6	2	2	0	2	0	0	2	2	-0.0035460992907801	-0.0565891472868217	0.142017351524997	-0.146632566069906	0.0112359550561797	0.336842105263158	-0.610378912685338	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLU	ERR2515591	ERR2515591	lineage2.2.1	za	South Africa	pnca_p.asp63glu	A	-	ASP	DA63	pnca_complex.pdb
157	D63G	L2	PZA	D	D63	63	G	1	Pre-MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	10.737	-0.799	-1.153	4.61622	-0.964	-0.66	33	66	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	0.0005749604714675	4.85515789473684	4.85515785897614	4.85442845338892	4.01139112494925	4	12	0	12	4	0	4	6	3	0	1	0	0	7	11	-0.202380952380952	-0.297932816537468	0.168132780687505	-0.164364876385337	-0.172774869109948	0.347368421052632	-0.610378912685338	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	1.58004161593687	0.0255237229775361	0.70746570614631	2.23338262506551	1.14726850666831	20.5466864308595	0.686203358177678	0.033386534669467	1.47642865587913	0.903495684237988	26.0627135254866	0.0451938468216249	lineage2	dr_mutations_pyrazinamide	GLY	SRR6369875	SRR6369875	lineage2.2.1	au	Australia	pnca_p.asp63gly	A	-	ASP	DA63	pnca_complex.pdb
158	D63H	L3	PZA	D	D63	63	H	0	XDR	DM	hydrophilic	hydrophobic	acidic	basic	neg	pos	10.737	-0.456	-0.085	4.09043	-0.703	0.25	35	66	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	0.0002156101768003	2.42493692178301	2.42493957425811	2.42473736947533	4.31445715363782e-25	-4	6	0	6	2	0	2	6	3	0	3	0	0	2	-2	-0.115501519756839	-0.0219638242894056	0.148982364382025	-0.119863597612958	0.140449438202247	0.368421052631579	-0.610378912685338	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	0.885806606247284	0.469406216824826	1.22442753211922	0.723445514749363	0.112662937620386	25.3270547471305	0.384700446081433	0.430258985168156	0.366270051463612	0.0410854444106247	46.5881472867033	0.999999999999476	lineage3	dr_mutations_pyrazinamide	HIS	ERR2510422	ERR2510422	lineage3	pk	Pakistan	pnca_p.asp63his	A	-	ASP	DA63	pnca_complex.pdb
159	D63V	L4	PZA	D	D63	63	V	NA	MDR	OM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	10.737	-0.722	0.0279999999999999	4.48687	-0.842	-0.12	40	71	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	NA	NA	NA	NA	NA	0	12	0	12	4	0	4	6	1	0	1	0	0	0	0	-0.182877406281662	0.0238095238095238	0.163421572126837	-0.143563512361466	-0.031413612565445	0.421052631578947	-0.610378912685338	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	VAL	SAMN02584627	SRR1166318_SRR1169048	lineage4.3.3	md	Moldova	pnca_p.asp63val	A	-	ASP	DA63	pnca_complex.pdb
160	D63Y	L2	PZA	D	D63	63	Y	NA	XDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	10.737	-0.438	0.07	4.37717	-0.53	0.23	35	66	-0.741	8	8	84	0.435233160621762	loop	-2.13333333333333	3.62	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-6	12	0	12	4	0	4	6	2	0	2	-2	0	2	0	-0.110942249240121	0.0595238095238095	0.15942605933901	-0.0903665814151748	0.129213483146067	0.368421052631579	-0.610378912685338	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.872	-0.674	8:7	125/150	W:D:M:G:N:F:Y:E	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987744	NA	4692673.51081431	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	TYR	SAMN02360617	SRR1049071_SRR1013674_SRR1013675_SRR1049070	lineage2.2.2	kr	South Korea	pnca_p.asp63tyr	A	-	ASP	DA63	pnca_complex.pdb
161	Y64D	L2	PZA	Y	Y64	64	D	0	MDR	DM	hydrophobic	hydrophilic	neutral	acidic	polar	neg	8.434	0.546	-0.392	2.59866	-0.33	0.17	69	80	0.947	1	1	135	0.513307984790874	loop	-1.86666666666667	3.61	0.0002874802357337	4.85191417753471	4.85191414039982	4.85118443551178	1.17497090855698	32	-18	0	-18	0	0	0	0	3	0	-3	4	6	6	4	0.244843049327354	-0.101291989664083	0.0946488537940982	-0.0562659846547315	0.0955056179775281	0.726315789473684	0.303525641025641	Stabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.59	1.183	3:1	146/150	E:Y:H:F:Q:N:R:K:S:G:A:T:P:W:D	1.57937329521209	0.114342297004304	1.0002536454007	1.57897279602454	0.581889091311512	40.4562911075298	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561	lineage2	dr_mutations_pyrazinamide	ASP	ERS457793	ERR552068_ERR552067	lineage2.2.1	uz	Nukus	pnca_p.tyr64asp	A	S	TYR	YA64	pnca_complex.pdb
162	S65P	L2	PZA	S	S65	65	P	0	Pre-XDR	OM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	8.268	-0.499	-0.032	3.33753	-0.186	-0.19	21	63	-0.697	8	8	97	0.625806451612903	loop	-0.966666666666667	3.79	0.0001437401178668	1.2119588062211	4.59700131334264e-05	0	1.01056822389159e-25	0	0	0	0	4	0	4	0	0	4	-4	-2	0	-2	-2	-0.126393110435664	-0.0082687338501291	0.121560107518266	-0.0317135549872123	-0.0497382198952879	0.221052631578947	-0.574135090609555	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.827	-0.615	8:7	147/150	L:V:R:K:I:E:Q:D:G:S:A:T	-9.98752126245058	0.942832958100734	139.277183325397	-0.0717096729269463	NA	144471.706314841	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	other_mutations_pyrazinamide	PRO	ERR552937,ERS458416,SAMEA2535014	ERR552937	lineage2.2.1	uz	Nukus	pnca_p.ser65pro	A	S	SER	SA65	pnca_complex.pdb
163	S66L	L1	PZA	S	S66	66	L	0	Sensitive	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	9.005	-0.663	0.201	1.22464	0.084	-0.57	32	66	-0.098	5	5	71	0.458064516129032	loop	-0.8	3.87	0.0005030904125341	0.346123821533658	1.69039306948873e-05	0	0.489658927611485	-10	0	0	0	2	0	0	4	-1	0	0	-5	0	0	0	-0.167933130699088	0.170918367346939	0.0446040545167141	0.070945945945946	-0.149214659685864	0.336842105263158	-0.0807248764415157	Destabilising	Stabilising	Destabilising	Stabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	N:V:I:R:H:F:E:Q:D:T:G:M:S:A	-10.9879643775828	0.928667766494711	122.741582014466	-0.0895211239520097	NA	1.02643587095793	-0.460768509909571	0.611380301705926	0.213688558083325	0	3.36442927964707	0.484079488471969	lineage1	other_mutations_pyrazinamide	LEU	ERR2514260	ERR2514260	lineage1.1.2	gb	UK	pnca_p.ser66leu	A	S	SER	SA66	pnca_complex.pdb
164	S66P	L4	PZA	S	S66	66	P	NA	Pre-MDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	9.005	-0.726	-0.248	1.91796	-0.165	-0.07	46	71	-0.098	5	5	71	0.458064516129032	loop	-0.8	3.87	7.18700589334483e-05	9.70142977291842	188179.926264563	Inf	0.763784339928386	0	0	0	0	4	0	2	4	2	4	-2	-4	0	-2	0	-0.183890577507599	-0.0640826873385013	0.0698562780906038	-0.0281329923273657	-0.0183246073298429	0.484210526315789	-0.0807248764415157	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	N:V:I:R:H:F:E:Q:D:T:G:M:S:A	12.1451538387261	0.919026337424221	119.468044436209	0.101660271548273	5.01153666064915e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	PRO	ERR1213862	ERR1213862	lineage4	kr	South Korea	pnca_p.ser66pro	A	S	SER	SA66	pnca_complex.pdb
165	S67L	L4	PZA	S	S67	67	L	NA	XDR	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	8.658	-1.544	0.61	0.0243891	-0.207	0.22	33	66	-0.355	6	6	15	0.0967741935483871	loop	-0.833333333333333	5.15	NA	NA	NA	NA	NA	-8	0	0	0	2	0	0	4	-5	0	-2	-5	0	0	-2	-0.391084093211753	0.518707482993197	0.000888304110606866	-0.0352941176470588	0.123595505617977	0.347368421052632	-0.292421746293245	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.553	-0.244	7:6	148/150	N:L:V:I:F:Q:W:T:A:M:S:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	LEU	ERR779901	ERR779901	lineage4.3.4.2	pe		pnca_p.ser67leu	A	-	SER	SA67	pnca_complex.pdb
166	S67P	L2	PZA	S	S67	67	P	1	MDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	8.658	-1.699	-0.228	3.29311	-0.86	0	40	71	-0.355	6	6	15	0.0967741935483871	loop	-0.833333333333333	5.15	0.0009343107661348	58.4740177439797	58.4739415306308	58.4547574725378	46.7514830195167	0	0	0	0	2	0	2	4	-2	2	-2	-4	0	0	-4	-0.430344478216819	-0.0589147286821705	0.119942234427698	-0.146632566069906	0	0.421052631578947	-0.292421746293245	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.553	-0.244	7:6	148/150	N:L:V:I:F:Q:W:T:A:M:S:P	4.06858121107736	9.19336449861209e-05	1.04030498364652	3.91095041842055	11.5166635599536	1065.61332397634	1.76696293519342	6.68324361543955e-09	8.17501270782547	8.64086529870195	2465.74647273772	8.05839318437163e-12	lineage2	dr_mutations_pyrazinamide	PRO	ERR2517333	ERR2517333	lineage2.2.1	nl	Netherlands	pnca_p.ser67pro	A	-	SER	SA67	pnca_complex.pdb
167	S67T	L1	PZA	S	S67	67	T	NA	Pre-MDR	OM	hydrophilic	hydrophobic	neutral	neutral	polar	polar	8.658	-1.302	-0.129	-0.265321	-0.27	-0.1	-47	72	-0.355	6	6	15	0.0967741935483871	loop	-0.833333333333333	5.15	NA	NA	NA	NA	NA	0	0	0	0	0	0	0	0	-8	0	-6	-2	0	0	-4	-0.329787234042553	-0.0333333333333333	-0.0510114973467661	-0.0460358056265985	-0.0261780104712041	-0.505376344086022	-0.292421746293245	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.553	-0.244	7:6	148/150	N:L:V:I:F:Q:W:T:A:M:S:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	THR	ERR702404	ERR702404	lineage1	gh	Ghana	pnca_p.ser67thr	A	-	SER	SA67	pnca_complex.pdb
168	S67W	L2	PZA	S	S67	67	W	NA	Pre-XDR	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	8.658	-0.833	-0.832	0.201358	-0.082	-0.65	39	66	-0.355	6	6	15	0.0967741935483871	loop	-0.833333333333333	5.15	7.18700589334483e-05	9.70142977291842	188179.926264541	Inf	0.763784339928386	-10	-4	0	-4	0	0	0	2	-4	0	-4	-4	0	0	-6	-0.210992907801418	-0.214987080103359	0.00733389666300016	-0.0139812446717818	-0.170157068062827	0.410526315789474	-0.292421746293245	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.244	7:6	148/150	N:L:V:I:F:Q:W:T:A:M:S:P	12.145153838726	0.919026337424218	119.468044436204	0.101660271548276	5.01153666065335e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	TRP	ERR2515719	ERR2515719	lineage2.2.1	za	South Africa	pnca_p.ser67trp	A	-	SER	SA67	pnca_complex.pdb
169	W68C	L4	PZA	W	W68	68	C	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	4.974	-1.575	-1.453	2.68091	-1.561	-1.46	78	85	-1.146	9	9	45	0.157894736842105	strand	-1.1	5.49	0.0004312203536006	24.2923336141533	24.2923334241599	24.2833096922897	14.1977442691074	46	0	0	0	0	0	0	0	4	0	0	4	2	0	4	-0.398936170212766	-0.375452196382429	0.0976445778305495	-0.26615515771526	-0.382198952879581	0.821052631578947	-0.943986820428336	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	150/150	F:W	3.19016080361872	0.0035954281300595	1.09565968954934	2.91163472932995	3.91551977432164	465.531063220521	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage4	dr_mutations_pyrazinamide	CYS	ERS2592795	ERR2677450	lineage4.6	de	Germany	pnca_p.trp68cys	A	B	TRP	WA68	pnca_complex.pdb
170	W68G	L2	PZA	W	W68	68	G	NA	XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	4.974	-2.125	-2.572	4.03792	-1.882	-3.08	86	91	-1.146	9	9	45	0.157894736842105	strand	-1.1	5.49	0.0013655311197355	87.9339263024142	87.9339260820995	87.8998400324811	75.5173867602406	52	0	0	0	0	0	0	0	4	0	0	4	4	5	21	-0.538247213779129	-0.664599483204134	0.147069835881672	-0.320886615515772	-0.806282722513089	0.905263157894737	-0.943986820428336	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	150/150	F:W	4.47658569248055	1.32290566590749e-05	1.02761623592683	4.35628159226488	18.1565285172187	1582.53238090538	1.94415646509946	2.3565519928076e-13	12.6277229739224	13.8702072448497	3588.35734387119	3.62203368500016e-18	lineage2	dr_mutations_pyrazinamide	GLY	SAMN02419585	SRR1159293_SRR1180989_SRR1181094_SRR1162491	lineage2.2.1	by	Belarus	pnca_p.trp68gly	A	B	TRP	WA68	pnca_complex.pdb
171	W68L	L2	PZA	W	W68	68	L	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	4.974	-2.242	-1.622	0.192132	-1.096	-1.35	84	91	-1.146	9	9	45	0.157894736842105	strand	-1.1	5.49	NA	NA	NA	NA	NA	30	0	0	0	0	0	0	0	-3	0	-1	-3	0	0	2	-0.567882472137791	-0.419121447028424	0.00699786566044333	-0.186871270247229	-0.353403141361257	0.884210526315789	-0.943986820428336	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	150/150	F:W	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	dr_mutations_pyrazinamide	LEU	BGI_SH415,SRR671826	SRR671826	lineage2.2.1	cn	China	pnca_p.trp68leu	A	B	TRP	WA68	pnca_complex.pdb
172	W68R	L2;L4	PZA	W	W68	68	R	NA	Pre-MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	4.974	-0.576	-1.608	0.0790982	-1.263	-1.5	89	91	-1.146	9	9	45	0.157894736842105	strand	-1.1	5.49	0.0020123616501365	132.405612244898	132.40553086776	132.608758225219	119.023328875182	-18	-32	0	-32	-2	0	-2	0	-1	0	-4	3	2	0	-2	-0.145896656534954	-0.415503875968992	0.00288092861981803	-0.215345268542199	-0.392670157068063	0.936842105263158	-0.943986820428336	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	150/150	F:W	4.88586941655608	1.59295080619135e-06	1.01805765099734	4.79920701128237	28.2126225203342	2362.8544732838	2.12190639382976	4.03470202806197e-20	19.3941885333947	21.793565475171	5246.53379470447	1.03505008297772e-27	lineage2;lineage4	dr_mutations_pyrazinamide	ARG	SRS797298	SRR1723828	lineage4;lineage2.2.1	cn	China:Hubei	pnca_p.trp68arg	A	B	TRP	WA68	pnca_complex.pdb
173	W68S	L2	PZA	W	W68	68	S	NA	XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	4.974	-1.036	-2.673	2.64788	-1.343	-3.09	86	91	-1.146	9	9	45	0.157894736842105	strand	-1.1	5.49	7.18700589334483e-05	9.70142977291842	188179.926264399	Inf	0.763784339928386	48	0	0	0	-2	0	-2	0	0	0	0	0	4	2	4	-0.26241134751773	-0.690697674418605	0.0964415533329934	-0.228985507246377	-0.808900523560209	0.905263157894737	-0.943986820428336	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	150/150	F:W	12.1451538387252	0.919026337424192	119.468044436157	0.101660271548309	5.01153666069715e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	SER	SAMN02360558	SRR1048856_SRR1048857_SRR1013557_SRR1013558	lineage2.2.2	kr	South Korea	pnca_p.trp68ser	A	B	TRP	WA68	pnca_complex.pdb
174	P69L	L2	PZA	P	P69	69	L	1	Pre-XDR	DM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	7.994	-1.774	0.046	2.773	-1.421	-0.28	71	85	-1.198	9	9	23	0.144654088050314	loop	-1.36666666666667	4.65	0.0004312203536006	24.2923336141533	24.2923334241601	24.2833096922897	14.1977442691074	-12	0	0	0	0	0	0	0	-1	0	-1	0	-4	-2	0	-0.449341438703141	0.0391156462585034	0.100998696086073	-0.24228473998295	-0.0732984293193717	0.747368421052632	-0.986820428336079	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	P	3.19016080361873	0.0035954281300594	1.09565968954934	2.91163472932996	3.91551977432241	465.531063220338	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage2	dr_mutations_pyrazinamide	LEU	ERS2592804	ERR2677460	lineage2.2.1	de	Germany	pnca_p.pro69leu	A	-	PRO	PA69	pnca_complex.pdb
175	P69Q	L3	PZA	P	P69	69	Q	0	Pre-MDR	OM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	7.994	-0.293	-1.59	2.83344	-1.339	-0.94	71	85	-1.198	9	9	23	0.144654088050314	loop	-1.36666666666667	4.65	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-12	0	0	0	-2	0	-2	0	-6	0	-2	-5	-8	-2	-2	-0.0742147922998986	-0.410852713178295	0.103200052447934	-0.228303495311168	-0.246073298429319	0.747368421052632	-0.986820428336079	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	P	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987744	NA	4692673.51081431	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	GLN	ERR3335782	ERR3335782	lineage3	pk	Pakistan	pnca_p.pro69gln	A	-	PRO	PA69	pnca_complex.pdb
176	P69R	L2	PZA	P	P69	69	R	NA	XDR	DM	hydrophilic	hydrophilic	non-polar	basic	non-polar	pos	7.994	-0.966	-1.258	2.33826	-1.319	-1.24	77	85	-1.198	9	9	23	0.144654088050314	loop	-1.36666666666667	4.65	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-46	-14	0	-14	-2	0	-2	0	-8	0	-2	-7	-6	0	-2	-0.24468085106383	-0.325064599483204	0.0851645189723119	-0.224893435635124	-0.324607329842932	0.810526315789474	-0.986820428336079	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	P	-8.98740878149642	0.940032862253634	119.468044435493	-0.0752285585987743	NA	4692673.51081303	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	dr_mutations_pyrazinamide	ARG	SAMN02628504	SRR1173192_SRR1173793	lineage2.2.1	ir	Iran	pnca_p.pro69arg	A	-	PRO	PA69	pnca_complex.pdb
177	P69S	L4	PZA	P	P69	69	S	NA	Pre-XDR	OM	hydrophilic	hydrophilic	non-polar	neutral	non-polar	polar	7.994	-0.353	-2.477	3.28838	-1.447	-1.93	73	85	-1.198	9	9	23	0.144654088050314	loop	-1.36666666666667	4.65	0.0001437401178668	1.2119588062211	4.59700131334263e-05	0	1.01056822389159e-25	0	0	0	0	0	0	0	-2	-1	0	-1	0	2	2	2	-0.0894123606889564	-0.640051679586563	0.119769957531742	-0.246717817561807	-0.505235602094241	0.768421052631579	-0.986820428336079	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	P	-9.98752126245059	0.942832958100734	139.277183325398	-0.0717096729269462	NA	144471.706314845	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage4	other_mutations_pyrazinamide	SER	ERR2707003	ERR2707003	lineage4.4.1.2	cd	Democratic Republic of Congo	pnca_p.pro69ser	A	-	PRO	PA69	pnca_complex.pdb
178	P69T	L4	PZA	P	P69	69	T	NA	Pre-MDR	OM	hydrophilic	hydrophobic	non-polar	neutral	non-polar	polar	7.994	-0.377	-1.905	2.75187	-1.735	-1.08	72	85	-1.198	9	9	23	0.144654088050314	loop	-1.36666666666667	4.65	7.18700589334483e-05	9.70142977291842	188179.926264408	Inf	0.763784339928386	0	0	0	0	-2	0	-2	0	-1	0	-1	0	0	2	0	-0.0954913880445795	-0.492248062015504	0.100229095491663	-0.295822676896846	-0.282722513089005	0.757894736842105	-0.986820428336079	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	P	12.1451538387252	0.919026337424193	119.46804443616	0.101660271548307	5.01153666069443e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	THR	ERR1213868	ERR1213868	lineage4.1.2.1	br	Brazil	pnca_p.pro69thr	A	-	PRO	PA69	pnca_complex.pdb
179	H71D	L2	PZA	H	H71	71	D	NA	Pre-XDR	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	4.184	-2.498	-2.685	5.74525	-1.66	-2.6	82	91	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	7.18700589334483e-05	9.70142977291842	188179.926264626	Inf	0.763784339928386	18	-24	0	-24	4	0	2	2	-3	0	-7	4	0	0	0	-0.632725430597771	-0.693798449612403	0.209254510886589	-0.283034953111679	-0.680628272251309	0.863157894736842	-0.995881383855025	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	12.1451538387264	0.919026337424233	119.468044436231	0.101660271548257	5.01153666062825e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	ASP	SRS797287	SRR1723816	lineage2.2.1	cn	China:Guangdong	pnca_p.his71asp	A	-	HIS	HA71	pnca_complex.pdb
180	H71N	L4	PZA	H	H71	71	N	NA	MDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	4.184	-1.335	-2.671	0.64378	-1.764	-2.6	70	85	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	NA	NA	NA	NA	NA	16	6	0	6	-2	0	2	-4	1	0	-2	5	0	0	0	-0.338145896656535	-0.690180878552972	0.0234478689384392	-0.30076726342711	-0.680628272251309	0.736842105263158	-0.995881383855025	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	ASN	SAMN02567731	SRR1166325	lineage4.3.3	ro	Romania	pnca_p.his71asn	A	-	HIS	HA71	pnca_complex.pdb
181	H71P	L4	PZA	H	H71	71	P	NA	MDR	OM	hydrophobic	hydrophilic	basic	non-polar	pos	non-polar	4.184	-2.885	-2.362	3.26218	-1.555	-2.44	84	91	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	0.0001437401178668	4.85029436501262	4.85029432717734	4.84956447904112	0.0881192621863332	32	8	0	8	2	0	2	0	-2	0	-4	2	0	0	0	-0.730749746707194	-0.610335917312661	0.118815696501286	-0.265132139812447	-0.638743455497382	0.884210526315789	-0.995881383855025	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673778	122.683760399245	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage4	other_mutations_pyrazinamide	PRO	3033	ERR2433011	lineage4.3.4.2.1	br	Momazbique	pnca_p.his71pro	A	-	HIS	HA71	pnca_complex.pdb
182	H71Q	L2	PZA	H	H71	71	Q	NA	XDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	4.184	-1.733	-2.294	1.12335	-1.091	-1.86	67	80	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	0.0001437401178668	19.411022297013	511754.463553472	Inf	4.73122070382274	-4	6	0	6	0	0	2	-2	-5	0	-5	-1	0	0	2	-0.438956433637285	-0.592764857881137	0.040914852235229	-0.186018755328218	-0.486910994764398	0.705263157894737	-0.995881383855025	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	13.1456002256222	0.924803768447052	139.277183326685	0.0943844491368574	0.0001628371344425	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	GLN	SAMN02360618	SRR1049072_SRR1013676_SRR1049073_SRR1013677	lineage2.2.1	kr	South Korea	pnca_p.his71gln	A	-	HIS	HA71	pnca_complex.pdb
183	H71R	L4	PZA	H	H71	71	R	NA	Pre-XDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	4.184	-0.831	-1.93	-1.52387	-1.26	-1.58	77	85	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	0.0005030904125341	1.94026083298275	1.94026661170037	1.94003614333864	0.0926662555126122	-36	-2	0	-2	0	0	2	-2	-1	0	-1	1	-4	-4	0	-0.21048632218845	-0.498708010335917	-0.292984311312774	-0.214833759590793	-0.413612565445026	0.810526315789474	-0.995881383855025	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	0.662825392344309	0.428026066477985	0.836294653807202	0.792573992105318	0.27776402289494	9.00753289954653	0.287860116894481	0.342335895194644	0.465547561079344	0.184637933513187	11.8604663966297	0.760814539944039	lineage4	dr_mutations_pyrazinamide	ARG	ERR3335745	ERR3335745	lineage4.5	pk	Pakistan	pnca_p.his71arg	A	-	HIS	HA71	pnca_complex.pdb
184	H71Y	L4	PZA	H	H71	71	Y	NA	Pre-XDR	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	4.184	-1.955	-0.461	-1.77532	-0.84	-0.23	61	80	-1.209	9	9	5	0.0223214285714285	loop	-0.766666666666667	6.25	0.0017967514733362	25.6733460559796	25.6733459339263	25.6638623299459	74.4270249019608	-26	-6	0	-6	-2	0	2	-4	-8	0	-3	-5	-12	-12	-6	-0.495187436676798	-0.119121447028424	-0.34132892409444	-0.143222506393862	-0.0602094240837696	0.642105263157895	-0.995881383855025	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	H	3.24545333052325	2.7826871320661e-09	0.546011377839736	5.94392985612077	9.76352440503871	88.06998811648	1.40948247478545	4.51976315688199e-13	12.3448843223466	8.64707452935555	102.96388402837	6.29217184975434e-18	lineage4	dr_mutations_pyrazinamide	TYR	ERR2516045	ERR2516045	lineage4.1.2.1	za	South Africa	pnca_p.his71tyr	A	-	HIS	HA71	pnca_complex.pdb
185	C72G	LBOV	PZA	C	C72	72	G	NA	Other	OM	hydrophobic	hydrophobic	neutral	neutral	non-polar	non-polar	7.053	-0.725	-1.759	1.3326	-1.459	-1.55	54	75	-1.104	9	9	0	0	loop	1.16666666666667	7.01	NA	NA	NA	NA	NA	10	0	0	0	0	0	0	0	-2	0	0	-2	0	3	17	-0.183637284701114	-0.454521963824289	0.0485361927170215	-0.248763853367434	-0.405759162303665	0.568421052631579	-0.909390444810544	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	150/150	G:A:C	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineageBOV	other_mutations_pyrazinamide	GLY	ERR553338	ERR553338	lineageBOV	cg	Congo	pnca_p.cys72gly	A	S	CYS	CA72	pnca_complex.pdb
186	C72R	L2	PZA	C	C72	72	R	1	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	7.053	0.152	-1.639	7.93343	-2.368	-1.28	87	91	-1.104	9	9	0	0	loop	1.16666666666667	7.01	0.0002156101768003	9.70466975178797	9.70465898653054	9.70194990589383	2.29168245016684	-68	-32	0	-32	-6	0	-6	-2	-16	0	-5	-13	-20	-8	-12	0.0681614349775784	-0.423514211886305	0.288952789574516	-0.403751065643649	-0.335078534031414	0.91578947368421	-0.909390444810544	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	150/150	G:A:C	2.27260607808817	0.0634455902260893	1.22442760158496	1.8560559033024	0.929172866365108	208.881603005161	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	dr_mutations_pyrazinamide	ARG	0180X-sc-2011-09-07T13:18:50Z-1276428,ERR067581	ERR067581	lineage2.2.1	ru	Russia,Samara	pnca_p.cys72arg	A	S	CYS	CA72	pnca_complex.pdb
187	C72W	L4	PZA	C	C72	72	W	1	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	7.053	0.265	-1.725	27.4558	-1.622	-1.13	85	91	-1.104	9	9	0	0	loop	1.16666666666667	7.01	0.0001437401178668	19.411022297013	511754.463552333	Inf	4.73122070382274	-46	0	0	0	-2	0	0	-2	-12	0	-12	-6	-16	-14	-8	0.118834080717489	-0.445736434108527	1	-0.276555839727195	-0.295811518324607	0.894736842105263	-0.909390444810544	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	150/150	G:A:C	13.14560022562	0.924803768446981	139.277183326528	0.0943844491369478	0.0001628371344461	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	TRP	SAMN10961405	SRR8651625	lineage4.4.2	cn	China:Wenzhou	pnca_p.cys72trp	A	S	CYS	CA72	pnca_complex.pdb
188	C72Y	L4	PZA	C	C72	72	Y	NA	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	polar	7.053	0.154	-1.564	15.4245	-1.249	-1.05	83	91	-1.104	9	9	0	0	loop	1.16666666666667	7.01	7.18700589334483e-05	9.70142977291842	188179.92626441	Inf	0.763784339928386	-46	-12	0	-12	-2	0	-2	0	-15	0	-4	-13	-18	-16	-14	0.0690582959641255	-0.404134366925065	0.561793865048551	-0.212958226768968	-0.274869109947644	0.873684210526316	-0.909390444810544	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.062	9:9	150/150	G:A:C	12.1451538387253	0.919026337424193	119.46804443616	0.101660271548307	5.0115366606943e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	TYR	ERR1035034	ERR1035034	lineage4.3.4.2.1	za	South Africa	pnca_p.cys72tyr	A	S	CYS	CA72	pnca_complex.pdb
189	V73D	L2	PZA	V	V73	73	D	NA	MDR	OM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	10.768	1.153	-1.148	1.11169	-2.613	-1.37	87	91	-0.743	8	8	46	0.264367816091954	strand	1.96666666666667	4.66	NA	NA	NA	NA	NA	-6	-50	0	-50	0	0	0	0	-2	0	-4	2	2	2	-2	0.517040358744395	-0.296640826873385	0.0404901696544992	-0.445524296675192	-0.358638743455497	0.91578947368421	-0.612026359143328	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	150/150	I:R:K:L:V:E:T:M	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	ASP	BGI_SH337,SRR671818	SRR671818	lineage2.2.1	cn	China	pnca_p.val73asp	A	B	VAL	VA73	pnca_complex.pdb
190	T76I	L2	PZA	T	T76	76	I	NA	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	14.032	-0.319	0.311	-0.781308	-0.733	-0.73	69	80	-0.548	7	7	56	0.325581395348837	loop	-0.9	3.94	0.0002156101768003	29.1287878787879	511969.848429101	Inf	9.2872640727157	-10	0	0	0	2	0	2	0	-6	0	-6	0	0	0	-4	-0.0808004052684903	0.264455782312925	-0.150216873029301	-0.124978687127025	-0.19109947643979	0.726315789473684	-0.451400329489292	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	-0.674	-0.485	7:7	150/150	Q:T:S:D	13.1460210125007	0.907969111549626	113.719344731963	0.115600569485217	0.183926936276972	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage2	dr_mutations_pyrazinamide	ILE	SAMN03653232,SRS935975	SRR2024927	lineage2.2.1	cn	China	pnca_p.thr76ile	A	S	THR	TA76	pnca_complex.pdb
191	T76P	L4	PZA	T	T76	76	P	NA	MDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	14.032	-0.382	-0.519	1.15155	-1.623	-0.09	78	85	-0.548	7	7	56	0.325581395348837	loop	-0.9	3.94	0.0017248814144027	24.4404408647732	24.4404407276145	24.4312693653994	69.8031277140286	0	0	0	0	2	0	2	0	-4	2	-6	-2	4	2	0	-0.096757852077001	-0.134108527131783	0.0419419576191552	-0.276726342710997	-0.0235602094240837	0.821052631578947	-0.451400329489292	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.485	7:7	150/150	Q:T:S:D	3.19623916718558	5.52153426461584e-09	0.548179859875324	5.83063954212496	9.23910743126347	84.0861153416749	1.38810903558899	2.23953024381857e-12	11.6498430682339	8.17279939949528	98.407580019827	6.55288933297668e-17	lineage4	dr_mutations_pyrazinamide	PRO	ERR1035324	ERR1035324	lineage4.6	br	Brazil	pnca_p.thr76pro	A	S	THR	TA76	pnca_complex.pdb
192	P77L	L1	PZA	P	P77	77	L	0	Sensitive	OM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.235	0.067	0.544	0.65747	-0.447	-0.2	18	59	3.113	1	1	94	0.591194968553459	helix	-0.9	3.75	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-10	0	0	0	0	0	0	0	-5	-2	-3	-1	0	0	-2	0.0300448430493273	0.462585034013606	0.023946488537941	-0.0762148337595908	-0.0523560209424083	0.189473684210526	0.99775641025641	Stabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	1.712	3.125	1:1	150/150	T:W:Q:E:F:R:L:V:N:P:G:S:A:D:Y:H:K	-8.98740878149639	0.940032862253632	119.468044435489	-0.075228558598776	NA	4692673.51081001	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	other_mutations_pyrazinamide	LEU	SRR2100651,SAMN03647824	SRR2100651	lineage1.1.2			pnca_p.pro77leu	A	G	PRO	PA77	pnca_complex.pdb
193	G78A	L2	PZA	G	G78	78	A	NA	XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	9.84	-0.912	0.335	1.30582	-1.378	-0.01	67	80	-0.882	8	8	8	0.0769230769230769	helix	-0.0666666666666667	4.83	NA	NA	NA	NA	NA	-4	0	0	0	0	0	0	0	-2	0	0	-2	-2	-7	-16	-0.231003039513678	0.284863945578231	0.047560806824059	-0.234953111679454	-0.0026178010471204	0.705263157894737	-0.726523887973641	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	G:E:N:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	ALA	BGI_FJ05195,SRR671759	SRR671759	lineage2.2.1	cn	China	pnca_p.gly78ala	A	G	GLY	GA78	pnca_complex.pdb
194	G78C	L1	PZA	G	G78	78	C	1	MDR	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	non-polar	9.84	-0.281	-1.038	2.40156	-1.186	-0.68	70	85	-0.882	8	8	8	0.0769230769230769	helix	-0.0666666666666667	4.83	0.0002156101768003	9.70466975178797	9.70465898653048	9.70194990589383	2.29168245016684	-10	0	0	0	-2	0	-2	0	-5	0	-1	-4	-4	-9	-19	-0.0711752786220871	-0.268217054263566	0.0874700427596355	-0.202216538789429	-0.178010471204189	0.736842105263158	-0.726523887973641	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	G:E:N:D	2.27260607808816	0.0634455902260901	1.22442760158496	1.8560559033024	0.929172866365334	208.881603004981	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage1	dr_mutations_pyrazinamide	CYS	SAMN03647573,SRR2100400	SRR2100400	lineage1.2.2			pnca_p.gly78cys	A	G	GLY	GA78	pnca_complex.pdb
195	G78R	L4	PZA	G	G78	78	R	NA	Pre-MDR	OM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	9.84	-0.84	-0.272	1.06499	-1.34	-0.13	84	91	-0.882	8	8	8	0.0769230769230769	helix	-0.0666666666666667	4.83	NA	NA	NA	NA	NA	-42	-20	0	-20	0	0	2	-4	-9	0	-5	-7	-6	-11	-13	-0.212765957446809	-0.0702842377260982	0.0387892540009761	-0.22847399829497	-0.0340314136125654	0.884210526315789	-0.726523887973641	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	G:E:N:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ARG	ERR2707074	ERR2707074	lineage4.6.1.2	cd	Democratic Republic of Congo	pnca_p.gly78arg	A	G	GLY	GA78	pnca_complex.pdb
196	G78S	L2	PZA	G	G78	78	S	1	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	9.84	0.215	-0.859	0.982763	-1.528	-0.4	65	80	-0.882	8	8	8	0.0769230769230769	helix	-0.0666666666666667	4.83	0.0002156101768003	9.70466975178797	9.7046589865305	9.70194990589383	2.29168245016684	-6	-2	0	-2	0	0	2	-2	-10	0	-8	-2	-2	-5	-19	0.0964125560538116	-0.221963824289406	0.0357943676745897	-0.260528559249787	-0.104712041884817	0.684210526315789	-0.726523887973641	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	G:E:N:D	2.27260607808817	0.0634455902260898	1.22442760158496	1.8560559033024	0.929172866365337	208.881603004981	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	dr_mutations_pyrazinamide	SER	ERR228039,2180W-sc-2012-10-11T13:38:26Z-1494203	ERR228039	lineage2.2.1	ru	Russia,Samara	pnca_p.gly78ser	A	G	GLY	GA78	pnca_complex.pdb
197	G78V	L4	PZA	G	G78	78	V	NA	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	9.84	-0.842	0.114	2.8237	-1.939	-0.82	77	85	-0.882	8	8	8	0.0769230769230769	helix	-0.0666666666666667	4.83	0.0001437401178668	19.411022297013	511754.463553282	Inf	4.73122070382274	-8	-2	0	-2	2	0	2	0	-17	0	-11	-10	-8	-9	-21	-0.213272543059777	0.096938775510204	0.102845300446536	-0.330605285592498	-0.214659685863874	0.810526315789474	-0.726523887973641	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	G:E:N:D	13.1456002256219	0.924803768447042	139.277183326662	0.0943844491368702	0.0001628371344429	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	VAL	ERR1199111	ERR1199111	lineage4.6.1.2	cd	Democratic Republic of the Congo	pnca_p.gly78val	A	G	GLY	GA78	pnca_complex.pdb
198	A79T	L4	PZA	A	A79	79	T	0	Sensitive	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	10.663	-0.176	-1.881	-0.0668707	-2.659	-1.58	69	80	-0.886	8	8	1	0.0077519379844961	helix	-0.7	5.8	0.0001437401178668	1.2119588062211	4.59700131334282e-05	0	1.01056822389159e-25	0	0	0	0	-2	0	-2	0	-1	0	-1	0	-2	-2	-5	-0.044579533941236	-0.486046511627907	-0.0128567830500654	-0.453367433930094	-0.413612565445026	0.726315789473684	-0.729818780889621	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	Q:G:S:E:A:C:V	-9.98752126245055	0.942832958100734	139.277183325396	-0.0717096729269465	NA	144471.70631481	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage4	other_mutations_pyrazinamide	THR	ERR2512531	ERR2512531	lineage4.6	gb	UK	pnca_p.ala79thr	A	G	ALA	AA79	pnca_complex.pdb
199	A79V	L3	PZA	A	A79	79	V	0	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.663	-1.403	-0.186	-0.825109	-1.839	-0.64	25	63	-0.886	8	8	1	0.0077519379844961	helix	-0.7	5.8	0.0003593502946672	0.484657419083649	1.69068633684892e-05	0	0.177777263899814	0	0	0	0	0	0	0	0	-1	0	-1	0	-2	-2	-5	-0.355369807497467	-0.0480620155038759	-0.158638198877182	-0.313554987212276	-0.167539267015707	0.263157894736842	-0.729818780889621	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	150/150	Q:G:S:E:A:C:V	-10.9877909019996	0.939691219428539	145.229797681911	-0.0756579646696576	NA	60.3389082676172	-0.314565134742567	0.596111875058711	0.224672226566594	0	5.29224350433385	0.673290424524484	lineage3	other_mutations_pyrazinamide	VAL	ERR2512680	ERR2512680	lineage3	gb	UK	pnca_p.ala79val	A	G	ALA	AA79	pnca_complex.pdb
200	F81C	L2	PZA	F	F81	81	C	1	XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	11.313	-1.003	-1.592	2.56753	-1.315	-1.85	55	75	-0.082	5	5	40	0.166666666666667	strand	-1.3	4.56	7.18700589334483e-05	9.70142977291842	188179.926264665	Inf	0.763784339928386	26	4	0	4	-2	0	-2	0	2	0	2	0	10	10	6	-0.254052684903749	-0.411369509043928	0.0935150314323385	-0.224211423699915	-0.484293193717278	0.578947368421053	-0.0675453047775947	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	0.083	6:5	150/150	F:M:Y:P:V:L:I	12.1451538387266	0.919026337424239	119.468044436241	0.101660271548249	5.01153666061863e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	CYS	ERR234657,2531C-sc-2012-10-11T13:40:33Z-1494335	ERR234657	lineage2.2.1	ru	Russia,Samara	pnca_p.phe81cys	A	B	PHE	FA81	pnca_complex.pdb
201	F81S	L2	PZA	F	F81	81	S	0	XDR	OM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	11.313	-0.596	-2.584	2.44385	-1.725	-2.58	31	66	-0.082	5	5	40	0.166666666666667	strand	-1.3	4.56	0.0001437401178668	1.2119588062211	4.59700131334266e-05	0	1.01056822389159e-25	32	4	0	4	-2	0	-2	0	6	0	2	4	12	10	8	-0.15096251266464	-0.667700258397933	0.0890103366137574	-0.294117647058824	-0.675392670157068	0.326315789473684	-0.0675453047775947	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	0.083	6:5	150/150	F:M:Y:P:V:L:I	-9.98752126245058	0.942832958100734	139.277183325397	-0.0717096729269465	NA	144471.70631483	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	other_mutations_pyrazinamide	SER	0190H-sc-2011-08-25T12:38:23Z-1262321,ERR067722	ERR067722	lineage2.2.1	ru	Russia,Samara	pnca_p.phe81ser	A	B	PHE	FA81	pnca_complex.pdb
202	F81V	L4	PZA	F	F81	81	V	1	Pre-MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.313	-1.967	-1.563	2.08972	-1.962	-1.92	56	75	-0.082	5	5	40	0.166666666666667	strand	-1.3	4.56	0.0003593502946672	1.21225820016821	1.21225918982515	1.212214074125	7.19297638498975e-25	32	4	0	4	0	0	0	0	4	0	2	2	10	8	6	-0.49822695035461	-0.403875968992248	0.0761121511666023	-0.334526854219949	-0.50261780104712	0.589473684210526	-0.0675453047775947	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	0.083	6:5	150/150	F:M:Y:P:V:L:I	0.192485717774876	0.863268405978748	1.11770505415734	0.172215126932565	0.0619550849552711	8.1990567174233	0.083595130527928	1	0	0.0246053772172713	12.260612593354	0.999999999999323	lineage4	dr_mutations_pyrazinamide	VAL	SRR2101519,SAMN03648766	SRR2101519	lineage4.4.1.1			pnca_p.phe81val	A	B	PHE	FA81	pnca_complex.pdb
203	H82D	L4	PZA	H	H82	82	D	NA	MDR	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	11.736	0.013	-0.198	1.78228	-1.413	0.3	63	80	-0.684	8	8	33	0.147321428571428	loop	-0.666666666666667	4.73	0.0001437401178668	4.85029436501262	4.85029432717735	4.84956447904112	0.0881192621863332	26	-20	0	-20	-2	0	-2	0	-1	0	1	0	4	4	-2	0.005829596412556	-0.0511627906976744	0.064914517151203	-0.240920716112532	0.168539325842697	0.663157894736842	-0.563426688632619	Stabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.827	-0.615	8:7	150/150	D:P:S:A:I:R:V:H	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673665	122.683760399245	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage4	dr_mutations_pyrazinamide	ASP	ERR751365	ERR751365	lineage4.3.3	pe		pnca_p.his82asp	A	-	HIS	HA82	pnca_complex.pdb
204	H82R	L4	PZA	H	H82	82	R	NA	MDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	11.736	-0.111	-0.663	-0.0040868	-0.923	-0.05	52	75	-0.684	8	8	33	0.147321428571428	loop	-0.666666666666667	4.73	0.0004312203536006	24.2923336141533	24.2923334241601	24.2833096922897	14.1977442691074	-34	-18	0	-18	0	0	2	-2	-5	0	2	-5	2	2	-2	-0.0281155015197568	-0.171317829457364	-0.000785741751903407	-0.157374254049446	-0.013089005235602	0.547368421052632	-0.563426688632619	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.827	-0.615	8:7	150/150	D:P:S:A:I:R:V:H	3.19016080361873	0.0035954281300594	1.09565968954934	2.91163472932996	3.915519774324	465.531063220231	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage4	dr_mutations_pyrazinamide	ARG	ERR2706933	ERR2706933	lineage4.3.4.1	cd	Democratic Republic of Congo	pnca_p.his82arg	A	-	HIS	HA82	pnca_complex.pdb
205	H82Y	LBOV	PZA	H	H82	82	Y	NA	Other	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	11.736	-0.973	0.591	-0.667302	-0.819	1.23	62	80	-0.684	8	8	33	0.147321428571428	loop	-0.666666666666667	4.73	NA	NA	NA	NA	NA	-26	-4	0	-4	0	0	0	0	-4	0	0	-6	-2	-2	-4	-0.24645390070922	0.502551020408163	-0.128297700530647	-0.139641943734015	0.691011235955056	0.652631578947368	-0.563426688632619	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-0.827	-0.615	8:7	150/150	D:P:S:A:I:R:V:H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineageBOV	dr_mutations_pyrazinamide	TYR	SRR5657460	SRR5657460	lineageBOV	mx		pnca_p.his82tyr	A	-	HIS	HA82	pnca_complex.pdb
206	L85P	L2	PZA	L	L85	85	P	NA	Pre-MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	12.428	-1.426	-1.891	8.82868	-1.285	-1.32	85	91	-0.734	8	8	9	0.044776119402985	loop	-0.166666666666667	5.61	0.0005030904125341	29.1630847029077	29.1630732435189	29.1293325978315	18.6717493808583	18	0	0	0	0	0	0	0	1	0	-1	-2	4	-2	8	-0.361195542046606	-0.488630490956072	0.321559743296498	-0.219096334185848	-0.345549738219895	0.894736842105263	-0.604612850082372	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	150/150	F:V:L:I	3.37290329402598	0.001789077082818	1.07995768762031	3.12318096596747	4.97876379054104	550.894323518834	1.46483345924118	0.0001485295621003	3.828187099348	3.53443753556636	1331.56873199938	1.55265977742841e-05	lineage2	dr_mutations_pyrazinamide	PRO	SRS797068	SRR1723570	lineage2.2.1	cn	China:InnerMongolia	pnca_p.leu85pro	A	S	LEU	LA85	pnca_complex.pdb
207	L85R	L2	PZA	L	L85	85	R	0	MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	12.428	-0.808	-1.371	2.99904	-1.503	-0.82	84	91	-0.734	8	8	9	0.044776119402985	loop	-0.166666666666667	5.61	0.0009343107661348	26.7869510135135	26.7869506340703	26.7708032978517	37.2159659891988	-50	-32	0	-32	0	0	0	0	-3	0	1	-5	-8	-6	-6	-0.204660587639311	-0.354263565891473	0.109231564915246	-0.256265984654731	-0.214659685863874	0.884210526315789	-0.604612850082372	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	150/150	F:V:L:I	3.28791485229524	1.90630753051074e-05	0.768996545261044	4.27559118770699	7.18677177006371	173.199151011595	1.42792328347145	1.9896368453757e-07	6.70122618511891	5.83859157504838	247.443798048456	1.05744462378296e-09	lineage2	dr_mutations_pyrazinamide	ARG	ERR2516799	ERR2516799	lineage2.2.1	gb	UK	pnca_p.leu85arg	A	S	LEU	LA85	pnca_complex.pdb
208	D86G	L4	PZA	D	D86	86	G	0	Other	OM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	17.129	-1.107	-0.094	2.19531	-0.796	0.24	15	59	1.31	1	1	97	0.50259067357513	loop	-0.133333333333333	3.88	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	4	14	0	14	4	0	2	2	3	0	1	2	2	3	13	-0.280395136778116	-0.0242894056847545	0.0799579688080478	-0.135720375106564	0.134831460674157	0.157894736842105	0.419871794871795	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.842	1.712	2:1	150/150	D:P:A:G:S:T:K:R:N:L:V:Q:E:F:H	-8.98740878149642	0.940032862253633	119.468044435491	-0.0752285585987753	NA	4692673.51081135	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	GLY	ERR2513489	ERR2513489	lineage4.1.2	gb	UK	pnca_p.asp86gly	A	-	ASP	DA86	pnca_complex.pdb
209	T87M	L4	PZA	T	T87	87	M	0	Pre-MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	17.594	-0.692	-0.4	-1.95982	-0.061	-0.37	-47	72	1.003	1	1	86	0.5	loop	-1.66666666666667	3.72	0.0007905706482679	0.220184187397302	6.21642915145485e-06	0	1.2237252074576	-22	0	0	0	2	0	2	0	-5	0	-1	-4	-2	-2	0	-0.175278622087133	-0.103359173126615	-0.376801507344459	-0.0104006820119352	-0.0968586387434555	-0.505376344086022	0.321474358974359	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	0.59	1.183	3:1	150/150	I:R:L:V:N:Q:F:E:W:T:K:H:Y:D:P:G:M:A:S	-11.9883149074727	0.940801856882948	161.432542873449	-0.0742620706710335	NA	0.975080220739854	-0.657213873251097	0.23001103975255	0.638251318814789	0	1.93160031351699	0.268630815579749	lineage4	other_mutations_pyrazinamide	MET	1322-04,ERS458560	ERR553130_ERR553131	lineage4.8			pnca_p.thr87met	A	-	THR	TA87	pnca_complex.pdb
210	I90S	L4	PZA	I	I90	90	S	1	XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	14.878	0.447	-2.286	4.11572	-2.665	-1.93	38	66	0.151	4	4	24	0.121827411167513	loop	0.933333333333333	4.88	0.0005030904125341	6.47410526315789	6.47410517670655	6.47281975099898	5.34879705543722	12	0	0	0	0	0	-2	0	-1	0	-1	0	0	0	0	0.200448430493273	-0.590697674418605	0.149903481231652	-0.454390451832907	-0.505235602094241	0.4	0.0483974358974359	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	P:S:G:A:W:D:E:F:C:I:L:V:N	1.86781040138793	0.0145043045254535	0.764079088633845	2.44452495713176	1.42623754737829	32.8877353882374	0.811179756363585	0.0192724555107824	1.71506294819644	1.09429403734416	44.2397805449942	0.0207365726835135	lineage4	dr_mutations_pyrazinamide	SER	ERR2041748	ERR2041748	lineage4.2.2.1	rs	Serbia	pnca_p.ile90ser	A	-	ILE	IA90	pnca_complex.pdb
211	I90T	L4	PZA	I	I90	90	T	1	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	14.878	0.462	-1.983	2.28269	-2.457	-1.55	42	71	0.151	4	4	24	0.121827411167513	loop	0.933333333333333	4.88	0.0002874802357337	14.5631313131313	14.5630810302278	14.5631012253236	5.81873994206135	10	0	0	0	0	0	-2	0	-1	0	-1	0	2	0	2	0.207174887892377	-0.512403100775194	0.0831405386111496	-0.418925831202046	-0.405759162303665	0.442105263157895	0.0483974358974359	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	P:S:G:A:W:D:E:F:C:I:L:V:N	2.67848962960141	0.0202679102821363	1.15384949648317	2.3213509541454	1.8636331786073	294.488177945833	1.16325476548539	0.0174140488202037	1.75910024233227	1.16837785201846	760.989759542733	0.0158563003544608	lineage4	other_mutations_pyrazinamide	THR	ERR2516485	ERR2516485	lineage4.3.4.2;lineage4.1.2.1	pe	Peru	pnca_p.ile90thr	A	-	ILE	IA90	pnca_complex.pdb
212	A92G	L1	PZA	A	A92	92	G	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	15.57	-1.692	-0.574	1.40665	-1.618	-0.96	12	59	0.318	4	4	11	0.0852713178294573	strand	0.833333333333333	4.62	NA	NA	NA	NA	NA	6	0	0	0	0	0	0	0	0	0	2	0	0	3	12	-0.428571428571428	-0.148320413436692	0.0512332549042461	-0.275873827791986	-0.25130890052356	0.126315789473684	0.101923076923077	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.083	0.389	5:4	150/150	I:R:V:L:Q:E:F:W:T:K:Y:H:P:M:S:A:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	GLY	ERR2679251	ERR2679251	lineage1.1.1			pnca_p.ala92gly	A	E	ALA	AA92	pnca_complex.pdb
213	A92P	L2	PZA	A	A92	92	P	0	MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	15.57	-1.787	0.14	0.275359	-2.918	0.76	9	53	0.318	4	4	11	0.0852713178294573	strand	0.833333333333333	4.62	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	2	0	2	0	0	0	0	-6	2	-2	-6	2	0	-3	-0.452634245187437	0.119047619047619	0.0100291741635647	-0.497527706734868	0.426966292134831	0.0947368421052632	0.101923076923077	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	0.083	0.389	5:4	150/150	I:R:V:L:Q:E:F:W:T:K:Y:H:P:M:S:A:G	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987756	NA	4692673.5108108	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	PRO	MDR_18428	SRR6824520	lineage2.2.1	cn	China: Taojiang County, Yiyang City, Hunan Province	pnca_p.ala92pro	A	E	ALA	AA92	pnca_complex.pdb
214	A92V	L1	PZA	A	A92	92	V	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.57	-1.782	0.941	-1.35941	-0.312	0.55	-40	66	0.318	4	4	11	0.0852713178294573	strand	0.833333333333333	4.62	NA	NA	NA	NA	NA	0	2	0	2	0	0	0	0	-6	0	0	-6	-4	-2	-3	-0.451367781155015	0.800170068027211	-0.261364685072676	-0.0531969309462916	0.308988764044944	-0.43010752688172	0.101923076923077	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	neutral	0.083	0.389	5:4	150/150	I:R:V:L:Q:E:F:W:T:K:Y:H:P:M:S:A:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	VAL	SRR5073850	SRR5073850	lineage1.1.1	vn	Vietnam	pnca_p.ala92val	A	E	ALA	AA92	pnca_complex.pdb
215	V93G	L4	PZA	V	V93	93	G	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	13.206	-0.958	-2.333	3.06107	-2.536	-2.72	41	71	-0.095	5	5	27	0.155172413793103	strand	2.93333333333333	4.76	NA	NA	NA	NA	NA	4	0	0	0	0	0	0	0	2	0	0	1	4	13	15	-0.242654508611955	-0.602842377260982	0.111490832538116	-0.432395566922421	-0.712041884816754	0.431578947368421	-0.0782537067545305	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	150/150	W:T:A:S:K:I:C:R:L:V:Q:H:Y:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLY	SAMN02584678	SRR1166143_SRR1169099	lineage4.2.1	md	Moldova	pnca_p.val93gly	A	E	VAL	VA93	pnca_complex.pdb
216	V93L	L4	PZA	V	V93	93	L	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.206	-1.213	-0.015	-1.8226	-0.026	-0.52	-1	53	-0.095	5	5	27	0.155172413793103	strand	2.93333333333333	4.76	NA	NA	NA	NA	NA	-14	0	0	0	-2	0	0	-2	-8	0	-5	-4	0	2	-2	-0.307244174265451	-0.003875968992248	-0.350419134045989	-0.00443307757885763	-0.136125654450262	-0.010752688172043	-0.0782537067545305	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.332	-0.038	6:5	150/150	W:T:A:S:K:I:C:R:L:V:Q:H:Y:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	LEU	ERR2516559	ERR2516559	lineage4.1.2.1	pe	Peru	pnca_p.val93leu	A	E	VAL	VA93	pnca_complex.pdb
217	F94C	L4	PZA	F	F94	94	C	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	10.621	-1.615	-1.595	4.52813	-3.58	-1.42	49	71	-0.13	5	5	0	0	strand	1.9	7.59	0.0002874802357337	14.5631313131313	14.5630810302278	14.5631012253236	5.81873994206135	28	4	0	4	0	0	-2	0	2	0	0	2	8	8	6	-0.409067882472138	-0.412144702842377	0.164924351138921	-0.610400682011935	-0.371727748691099	0.515789473684211	-0.107084019769357	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	150/150	A:F:Q:V:L:I	2.67848962960141	0.0202679102821361	1.15384949648317	2.3213509541454	1.86363317860726	294.488177945833	1.16325476548539	0.0174140488202037	1.75910024233227	1.16837785201846	760.989759542733	0.0158563003544608	lineage4	dr_mutations_pyrazinamide	CYS	ERR1035329	ERR1035329	lineage4.3.3	br	Brazil	pnca_p.phe94cys	A	E	PHE	FA94	pnca_complex.pdb
218	F94L	L4	PZA	F	F94	94	L	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.621	-1.712	-1.582	1.1796	-2.563	-1.99	24	63	-0.13	5	5	0	0	strand	1.9	7.59	0.0005030904125341	6.47410526315789	6.47410517670654	6.47281975099898	5.34879705543722	16	4	0	4	0	0	0	0	-3	0	-1	-3	2	6	2	-0.433637284701115	-0.408785529715762	0.0429635996765711	-0.436999147485081	-0.520942408376963	0.252631578947368	-0.107084019769357	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	150/150	A:F:Q:V:L:I	1.86781040138793	0.0145043045254536	0.764079088633845	2.44452495713176	1.42623754737775	32.8877353882363	0.811179756363585	0.0192724555107824	1.71506294819644	1.09429403734416	44.2397805449942	0.0207365726835135	lineage4	dr_mutations_pyrazinamide	LEU	ERR2516686	ERR2516686	lineage4.1.1	pe	Peru	pnca_p.phe94leu	A	E	PHE	FA94	pnca_complex.pdb
219	F94S	L2	PZA	F	F94	94	S	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	10.621	-0.449	-3.027	5.85272	-3.24	-2.74	69	80	-0.13	5	5	0	0	strand	1.9	7.59	0.0001437401178668	19.411022297013	511754.463552622	Inf	4.73122070382274	32	4	0	4	0	0	-2	0	-1	0	-3	0	6	10	6	-0.113728470111449	-0.782170542635659	0.213168802220296	-0.552429667519182	-0.717277486910995	0.726315789473684	-0.107084019769357	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	150/150	A:F:Q:V:L:I	13.1456002256206	0.924803768446999	139.277183326568	0.0943844491369244	0.0001628371344452	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	SER	SAMN07236524,SRR5709885	SRR5709885	lineage2.2.1	th	Thailand	pnca_p.phe94ser	A	E	PHE	FA94	pnca_complex.pdb
220	F94V	L4	PZA	F	F94	94	V	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.621	-1.681	-1.995	2.56655	-2.927	-1.82	-5	53	-0.13	5	5	0	0	strand	1.9	7.59	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	30	4	0	4	0	0	0	0	-1	0	-3	2	4	6	2	-0.425785207700101	-0.515503875968992	0.0934793376991382	-0.499062233589088	-0.476439790575916	-0.0537634408602151	-0.107084019769357	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.332	-0.038	6:5	150/150	A:F:Q:V:L:I	-8.9874087814964	0.940032862253633	119.46804443549	-0.0752285585987757	NA	4692673.51081231	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	VAL	2591	ERR2432995	lineage4.3.3	br	Brazil	pnca_p.phe94val	A	E	PHE	FA94	pnca_complex.pdb
221	Y95D	L2	PZA	Y	Y95	95	D	NA	Pre-XDR	OM	hydrophobic	hydrophilic	neutral	acidic	polar	neg	10.528	0.071	-0.772	4.78106	-2.558	-0.4	46	71	-0.013	5	5	75	0.285171102661597	strand	-0.8	4.15	7.18700589334483e-05	9.70142977291842	188179.926264427	Inf	0.763784339928386	38	-12	0	-12	2	0	0	2	1	0	1	1	4	2	2	0.0318385650224215	-0.199483204134367	0.174136612300498	-0.43614663256607	-0.104712041884817	0.484210526315789	-0.0107084019769357	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	150/150	K:Y:H:D:G:S:M:P:L:N:C:R:E:F:Q:T	12.1451538387253	0.919026337424197	119.468044436166	0.101660271548303	5.01153666068889e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ASP	ERR2516654	ERR2516654	lineage2.2.1.1	pe	Peru	pnca_p.tyr95asp	A	E	TYR	YA95	pnca_complex.pdb
222	K96E	L2	PZA	K	K96	96	E	NA	Pre-XDR	DM	hydrophobic	hydrophilic	basic	acidic	pos	neg	3.977	-0.673	-2.119	6.92487	-2.7	-1.84	89	91	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	0.0007905706482679	107.166385135135	3795803.48339079	Inf	47.6847963834956	26	-2	0	-2	4	0	-4	4	0	0	-2	1	-2	0	-2	-0.170466058763931	-0.547545219638243	0.252218839006694	-0.460358056265985	-0.481675392670157	0.936842105263158	-0.983525535420099	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	15.1494066679806	0.925233417916433	161.43254287942	0.0938435732830911	24.1993753459371	NA	2.03005858162844	3.58120158191569e-09	8.44597123233921	12.2081619622864	Inf	5.00558510128865e-12	lineage2	dr_mutations_pyrazinamide	GLU	SAMN03653224,SRS935964	SRR2024918	lineage2.2.1	cn	China	pnca_p.lys96glu	A	E	LYS	KA96	pnca_complex.pdb
223	K96M	L6	PZA	K	K96	96	M	NA	MDR	OM	hydrophobic	hydrophobic	basic	non-polar	pos	non-polar	3.977	-1.026	0.413	0.273257	-0.457	1.02	84	91	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	0.0001437401178668	19.411022297013	511754.463553382	Inf	4.73122070382274	24	32	0	32	4	0	0	4	-5	0	-5	0	-2	-2	-4	-0.259878419452887	0.351190476190476	0.0099526147480678	-0.077919863597613	0.573033707865168	0.884210526315789	-0.983525535420099	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	13.1456002256221	0.924803768447045	139.277183326669	0.0943844491368667	0.0001628371344428	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage6	other_mutations_pyrazinamide	MET	ERR181315	ERR181315	lineage6	so	Somalia	pnca_p.lys96met	A	E	LYS	KA96	pnca_complex.pdb
224	K96N	L2	PZA	K	K96	96	N	0	MDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	3.977	0.325	-1.156	2.61245	-3.151	-0.85	90	95	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	52	6	0	6	4	0	2	0	-3	0	-1	-3	6	4	-2	0.145739910313901	-0.298708010335917	0.0951511156112734	-0.537254901960784	-0.222513089005236	0.947368421052632	-0.983525535420099	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987751	NA	4692673.51081327	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	dr_mutations_pyrazinamide	ASN	MDR_19128	SRR6824521	lineage2.2.1	cn	China: Zengcheng City, Guangzhou City, Guangdong Province	pnca_p.lys96asn	A	E	LYS	KA96	pnca_complex.pdb
225	K96Q	L2	PZA	K	K96	96	Q	0	Pre-XDR	DM	hydrophobic	hydrophilic	basic	neutral	pos	polar	3.977	-0.08	-1.318	1.04409	-1.359	-0.86	78	85	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	0.0002874802357337	4.85191417753471	4.85191414039981	4.85118443551178	1.17497090855698	26	8	0	8	0	0	-2	0	-2	0	-2	-1	2	2	-2	-0.0202634245187436	-0.340568475452196	0.0380280305072152	-0.231713554987212	-0.225130890052356	0.821052631578947	-0.983525535420099	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	1.57937329521209	0.114342297004304	1.0002536454007	1.57897279602454	0.58188909131153	40.4562911075298	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561	lineage2	dr_mutations_pyrazinamide	GLN	ERR2517049	ERR2517049	lineage2.2.1	gb	UK	pnca_p.lys96gln	A	E	LYS	KA96	pnca_complex.pdb
226	K96R	L2	PZA	K	K96	96	R	0	Pre-XDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	3.977	0.083	-0.169	-0.740521	-2.045	-0.35	85	91	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	0.0010780508840017	19.4879594423321	19.4879432617414	19.4791388432651	37.591449001387	-12	-4	0	-4	0	0	-2	2	-5	0	-3	-5	-8	-6	-8	0.037219730941704	-0.0436692506459948	-0.142375028839499	-0.348678601875533	-0.0916230366492146	0.894736842105263	-0.983525535420099	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	2.9697959800773	4.2195571364394e-06	0.645570763650269	4.60026405670093	6.18542852926305	85.5881348249979	1.28976636711505	1.66262776179573e-07	6.77920497212353	5.25121653678864	107.844057222035	8.72260296739213e-10	lineage2	dr_mutations_pyrazinamide	ARG	ERR2516844	ERR2516844	lineage2.2.1	gb	UK	pnca_p.lys96arg	A	E	LYS	KA96	pnca_complex.pdb
227	K96T	L2	PZA	K	K96	96	T	NA	Pre-XDR	DM	hydrophobic	hydrophobic	basic	neutral	pos	polar	3.977	-0.57	-0.86	3.53764	-0.932	-0.61	85	91	-1.194	9	9	8	0.0338983050847457	strand	-1.86666666666667	5.96	0.0009343107661348	58.4740177439797	58.473941530631	58.4547574725378	46.7514830195167	72	8	0	8	4	0	0	4	0	0	0	0	8	6	4	-0.144376899696049	-0.222222222222222	0.128848549304701	-0.158908780903666	-0.159685863874345	0.894736842105263	-0.983525535420099	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	150/150	K:Q	4.06858121107736	9.193364498612e-05	1.04030498364652	3.91095041842056	11.5166635599539	1065.61332397744	1.76696293519342	6.68324361543955e-09	8.17501270782547	8.64086529870195	2465.74647273772	8.05839318437163e-12	lineage2	dr_mutations_pyrazinamide	THR	SAMN02360569	SRR1048963_SRR1013580_SRR1013579_SRR1048962	lineage2.2.1	kr	South Korea	pnca_p.lys96thr	A	E	LYS	KA96	pnca_complex.pdb
228	G97A	L4	PZA	G	G97	97	A	NA	MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	8.236	-1.721	-1.126	3.72826	-1.921	-0.73	66	80	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	NA	NA	NA	NA	NA	-6	0	0	0	0	0	2	-2	-4	0	0	-4	-2	-1	-16	-0.435916919959473	-0.290956072351421	0.135791344633921	-0.327536231884058	-0.19109947643979	0.694736842105263	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ALA	SAMN02231160	SRR998769_SRR998771_SRR998770_SRR998768	lineage4.1	ml	Mali	pnca_p.gly97ala	A	-	GLY	GA97	pnca_complex.pdb
229	G97C	L4	PZA	G	G97	97	C	1	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	non-polar	8.236	-1.158	-1.635	4.47159	-1.484	-0.7	69	80	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	0.0007187005893344	97.3828619670747	1395803.74890115	Inf	42.8452490786805	-10	-2	0	-2	0	0	2	-2	-9	0	-3	-11	-8	-7	-21	-0.293313069908815	-0.422480620155039	0.162865041266326	-0.253026427962489	-0.183246073298429	0.726315789473684	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	14.1489809714057	0.890414731995348	102.692976013979	0.137779442378606	507.712885369765	NA	1.98848253379833	2.1018584548314e-08	7.67739653395739	10.9022485555592	Inf	5.92457926729285e-11	lineage4	dr_mutations_pyrazinamide	CYS	ERR551166	ERR551166	lineage4.1.1.1	sz	Swaziland	pnca_p.gly97cys	A	-	GLY	GA97	pnca_complex.pdb
230	G97D	L3;L4	PZA	G	G97	97	D	NA	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	8.236	-0.248	-2.974	12.1049	-2.263	-1.49	83	91	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	0.0025154520626706	29.4508301404853	29.4508186631222	29.4177544871982	111.74678026952	-16	-66	0	-66	-6	0	0	-6	-13	0	-9	-13	-8	-15	-23	-0.0628166160081053	-0.768475452196382	0.440886807159143	-0.385848252344416	-0.390052356020942	0.873684210526316	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	3.38272170811945	2.59985209607615e-12	0.483396852654822	6.9978149206837	12.4656214812387	86.4946048523286	1.46909754090074	1.11009505519018e-18	17.9546398318646	11.2940206861184	97.32420575844	4.05989172191837e-26	lineage3;lineage4	dr_mutations_pyrazinamide	ASP	ERR1035046	ERR1035046	lineage4.7;lineage3	za	South Africa	pnca_p.gly97asp	A	-	GLY	GA97	pnca_complex.pdb
231	G97R	L2	PZA	G	G97	97	R	1	MDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	8.236	-0.89	-1.252	4.89479	-2.091	-0.79	82	91	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	0.0001437401178668	19.411022297013	511754.463553323	Inf	4.73122070382274	-78	-34	0	-34	-4	0	0	-6	-16	0	-10	-15	-16	-13	-27	-0.225430597771023	-0.323514211886305	0.178278906460566	-0.356521739130435	-0.206806282722513	0.863157894736842	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	13.1456002256219	0.924803768447043	139.277183326664	0.0943844491368691	0.0001628371344429	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	ARG	ERR2517307	ERR2517307	lineage2.2.1	nl	Netherlands	pnca_p.gly97arg	A	-	GLY	GA97	pnca_complex.pdb
232	G97S	L4	PZA	G	G97	97	S	NA	XDR	DM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	8.236	-0.309	-1.999	5.99386	-2.075	-1.28	72	85	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	0.000646830530401	87.6075949367089	1395214.80217992	Inf	38.0099632572142	-6	0	0	0	-4	0	-2	-2	-7	0	-3	-4	-4	-7	-19	-0.0782674772036474	-0.516537467700258	0.21830942824467	-0.353793691389599	-0.335078534031414	0.757894736842105	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	14.1485589414412	0.896008552350388	108.247901040098	0.130705157379451	226.474535582214	NA	1.94254175795031	1.23317897951184e-07	6.90897388676731	9.59561062779463	Inf	7.038428913028e-10	lineage4	dr_mutations_pyrazinamide	SER	ERR688043	ERR688043	lineage4.8	pk	Pakistan	pnca_p.gly97ser	A	-	GLY	GA97	pnca_complex.pdb
233	G97V	L1	PZA	G	G97	97	V	NA	Pre-MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	8.236	-1.664	-0.588	6.12241	-2.616	-1.24	77	85	-1.026	9	9	7	0.0673076923076923	loop	-0.833333333333333	4.92	7.18700589334483e-05	9.70142977291842	188179.926264625	Inf	0.763784339928386	-8	-2	0	-2	-2	0	0	-2	-14	0	-8	-9	-12	-11	-23	-0.421479229989868	-0.151937984496124	0.22299149906395	-0.446035805626598	-0.324607329842932	0.810526315789474	-0.845140032948929	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.124	-0.993	9:9	150/150	A:G	12.1451538387264	0.919026337424232	119.468044436229	0.101660271548258	5.01153666063045e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage1	other_mutations_pyrazinamide	VAL	ERR1023294	ERR1023294	lineage1.2.1			pnca_p.gly97val	A	-	GLY	GA97	pnca_complex.pdb
234	T100A	L3	PZA	T	T100	100	A	0	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	8.272	-1.759	-0.543	0.408316	0.129	-0.1	-36	66	1.347	1	1	81	0.470930232558139	loop	-0.8	4.18	0.0002874802357337	4.85191417753471	4.85191414039982	4.85118443551178	1.17497090855698	0	0	0	0	2	0	2	0	0	0	0	0	2	2	5	-0.445542046605876	-0.14031007751938	0.0148717575157162	0.108952702702703	-0.0261780104712041	-0.387096774193548	0.431730769230769	Destabilising	Destabilising	Destabilising	Stabilising	Destabilising	neutral	0.842	1.712	2:1	150/150	K:Y:D:P:A:G:S:R:I:V:L:N:Q:F:E:T	1.57937329521209	0.114342297004304	1.0002536454007	1.57897279602454	0.581889091312076	40.456291107557	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561	lineage3	other_mutations_pyrazinamide	ALA	ERR2510467	ERR2510467	lineage3	pk	Pakistan	pnca_p.thr100ala	A	S	THR	TA100	pnca_complex.pdb
235	T100I	L2	PZA	T	T100	100	I	1	XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	8.272	-1.537	0.379	0.808015	-0.042	-0.36	14	59	1.347	1	1	81	0.470930232558139	loop	-0.8	4.18	0.000646830530401	38.9169126950654	38.9169126367816	38.9145296299157	27.8738145664613	-6	2	0	2	2	0	2	0	-1	0	-3	0	0	0	-2	-0.389311043566363	0.322278911564626	0.0294296651345071	-0.00716112531969309	-0.094240837696335	0.147368421052632	0.431730769230769	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	0.842	1.712	2:1	150/150	K:Y:D:P:A:G:S:R:I:V:L:N:Q:F:E:T	3.66142892829214	0.0005578275078108	1.06087424525349	3.45133171502081	7.13685172054609	722.165073757354	1.59013838008863	5.5228318480997e-06	5.25783817940065	5.21074959149254	1710.75565736304	1.29490403629991e-07	lineage2	dr_mutations_pyrazinamide	ILE	ERR1873474	ERR1873474	lineage2.2.1	za	South Africa	pnca_p.thr100ile	A	S	THR	TA100	pnca_complex.pdb
236	T100P	L2	PZA	T	T100	100	P	NA	MDR	OM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	8.272	-1.685	-0.488	1.65141	-0.412	0.03	18	59	1.347	1	1	81	0.470930232558139	loop	-0.8	4.18	0.0005030904125341	68.0817875210792	1394038.39846607	Inf	28.35828361563	0	0	0	0	2	0	2	0	0	2	0	-2	0	-2	-4	-0.426798378926039	-0.12609819121447	0.0601479468818974	-0.0702472293265132	0.0168539325842696	0.189473684210526	0.431730769230769	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.842	1.712	2:1	150/150	K:Y:D:P:A:G:S:R:I:V:L:N:Q:F:E:T	14.1477154154513	0.908235680148	122.741582019402	0.115264242017142	22.9578143501725	NA	1.83303094984313	4.24048522418865e-06	5.37258444573496	7.00237574052121	Inf	1.00814491180914e-07	lineage2	other_mutations_pyrazinamide	PRO	SAMN02403469	SRR1181050_SRR1180926	lineage2.2.1	za	South Africa	pnca_p.thr100pro	A	S	THR	TA100	pnca_complex.pdb
237	A102P	L4	PZA	A	A102	102	P	1	MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	3.5	-0.23	-1.249	-0.616782	-2.115	0.13	83	91	-0.862	8	8	10	0.0775193798449612	loop	0.0333333333333332	5.51	0.0005749604714675	14.5802781289507	14.5802277940116	14.5801759034061	15.0663448164144	0	0	0	0	0	0	2	-2	-8	0	-2	-6	-6	-2	-5	-0.058257345491388	-0.322739018087855	-0.118584557409828	-0.360613810741688	0.0730337078651685	0.873684210526316	-0.71004942339374	Destabilising	Destabilising	Stabilising	Destabilising	Stabilising	effect	-0.955	-0.827	9:8	150/150	M:E:S:G:A:H:Q	2.6796663501904	0.0010245352377103	0.816050632122414	3.28370108999368	3.35697961122126	99.5538692592317	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage4	other_mutations_pyrazinamide	PRO	ERR2041708	ERR2041708	lineage4	rs	Serbia	pnca_p.ala102pro	A	-	ALA	AA102	pnca_complex.pdb
238	A102R	L2	PZA	A	A102	102	R	0	Pre-XDR	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	3.5	0.174	-0.696	4.1344	-2.095	-0.28	81	91	-0.862	8	8	10	0.0775193798449612	loop	0.0333333333333332	5.51	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-76	-30	0	-30	-2	0	0	-2	-12	0	-4	-10	-16	-12	-7	0.0780269058295964	-0.17984496124031	0.150583847493062	-0.357203751065644	-0.0732984293193717	0.852631578947368	-0.71004942339374	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.955	-0.827	9:8	150/150	M:E:S:G:A:H:Q	-8.98740878149642	0.940032862253633	119.468044435492	-0.0752285585987749	NA	4692673.51081367	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	ARG	1323P-sc-2011-10-12T14:59:30Z-1289168,ERR108503	ERR108503	lineage2.2.1	ru	Russia,Samara	pnca_p.ala102arg	A	-	ALA	AA102	pnca_complex.pdb
239	A102T	L2	PZA	A	A102	102	T	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	3.5	0.881	-0.719	-2.02927	-1.769	-0.2	71	85	-0.862	8	8	10	0.0775193798449612	loop	0.0333333333333332	5.51	0.0001437401178668	19.411022297013	511754.463552419	Inf	4.73122070382274	-2	0	0	0	-2	0	0	-2	-5	0	-3	-3	-4	-2	-5	0.395067264573991	-0.18578811369509	-0.390154195185726	-0.301619778346121	-0.0523560209424083	0.747368421052632	-0.71004942339374	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.955	-0.827	9:8	150/150	M:E:S:G:A:H:Q	13.1456002256202	0.924803768446987	139.277183326542	0.0943844491369395	0.0001628371344458	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	THR	ERR234630,0365Y-sc-2012-10-11T13:37:02Z-1494116	ERR234630	lineage2.2.1	ru	Russia,Samara	pnca_p.ala102thr	A	-	ALA	AA102	pnca_complex.pdb
240	A102V	L2	PZA	A	A102	102	V	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	3.5	-0.162	-0.251	-1.90987	-1.389	-0.73	73	85	-0.862	8	8	10	0.0775193798449612	loop	0.0333333333333332	5.51	0.000646830530401	2.42613636363636	2.42613901795158	2.42593660187249	0.724684701500429	-2	-26	0	-26	0	0	0	0	-5	0	-3	-2	-4	-4	-3	-0.0410334346504559	-0.0648578811369509	-0.367197954318234	-0.236828644501279	-0.19109947643979	0.768421052631579	-0.71004942339374	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.955	-0.827	9:8	150/150	M:E:S:G:A:H:Q	0.886301112240174	0.210089452509977	0.707162944655621	1.25331950569298	0.511701209936356	9.20262713399942	0.384915207210874	0.188417082130056	0.724879726073294	0.392299288705794	11.3662890368022	0.394611528192705	lineage2	other_mutations_pyrazinamide	VAL	ERR751361	ERR751361	lineage2.2.1	pe		pnca_p.ala102val	A	-	ALA	AA102	pnca_complex.pdb
241	Y103C	L4	PZA	Y	Y103	103	C	0	Pre-MDR	DM	hydrophobic	hydrophobic	neutral	neutral	polar	non-polar	5.416	-0.48	0.606	1.02924	-0.498	1.43	68	80	-1.175	9	9	138	0.524714828897338	loop	-0.1	3.94	0.0007187005893344	3.23621052631579	3.23621068095208	3.23583471755484	2.2626260572101	42	4	0	4	0	0	0	0	0	0	-2	2	0	-2	0	-0.121580547112462	0.51530612244898	0.0374871611827009	-0.0849104859335038	0.803370786516854	0.715789473684211	-0.967874794069193	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.152	9:9	150/150	Y:K	1.17440310243918	0.0690022741521524	0.64584247435876	1.8184048728062	0.826804878959969	11.3377530375619	0.510036766167445	0.0754031320762053	1.12261061414134	0.671078964694216	13.6617156135614	0.132529157572648	lineage4	dr_mutations_pyrazinamide	CYS	ERR2516653	ERR2516653	lineage4.8	nl	Netherlands	pnca_p.tyr103cys	A	-	TYR	YA103	pnca_complex.pdb
242	Y103D	L2	PZA	Y	Y103	103	D	1	MDR	OM	hydrophobic	hydrophilic	neutral	acidic	polar	neg	5.416	0.851	1.176	1.18458	-0.578	1.51	88	91	-1.175	9	9	138	0.524714828897338	loop	-0.1	3.94	0.0021561017680034	142.334468085106	142.334276537658	142.74783870505	128.720903887203	34	-24	0	-24	0	0	0	0	2	0	-2	4	-2	-2	-2	0.381614349775785	1	0.0431449821167112	-0.0985507246376812	0.848314606741573	0.926315789473684	-0.967874794069193	Stabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.152	9:9	150/150	Y:K	4.95817835126683	1.0743991920355e-06	1.01654133277845	4.87749803317387	30.4620917601388	2537.05252258235	2.1533100827021	1.23916669217761e-21	20.9068702685848	23.5556923218963	5610.51758528632	7.80576752108032e-30	lineage2	other_mutations_pyrazinamide	ASP	15MT_1210	SRR5551225	lineage2.2.1.1	pg		pnca_p.tyr103asp	A	-	TYR	YA103	pnca_complex.pdb
243	Y103H	L2	PZA	Y	Y103	103	H	NA	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	basic	polar	pos	5.416	-0.046	0.779	0.909501	-0.683	1.21	81	91	-1.175	9	9	138	0.524714828897338	loop	-0.1	3.94	0.0001437401178668	19.411022297013	511754.463552419	Inf	4.73122070382274	22	-22	0	-22	-2	0	-2	0	3	0	-1	4	0	0	0	-0.0116514690982776	0.662414965986395	0.0331260061626323	-0.116453537936914	0.679775280898876	0.852631578947368	-0.967874794069193	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.152	9:9	150/150	Y:K	13.1456002256202	0.924803768446986	139.27718332654	0.0943844491369405	0.0001628371344458	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	HIS	SRR5709897,SAMN07236308	SRR5709897	lineage2.1	th	Thailand	pnca_p.tyr103his	A	-	TYR	YA103	pnca_complex.pdb
244	Y103S	L2	PZA	Y	Y103	103	S	NA	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	5.416	-0.062	0.233	1.16175	-0.796	0.42	85	91	-1.175	9	9	138	0.524714828897338	loop	-0.1	3.94	7.18700589334483e-05	9.70142977291842	188179.926264673	Inf	0.763784339928386	44	4	0	4	0	0	0	0	4	0	0	4	2	0	2	-0.0157041540020263	0.19812925170068	0.0423134638218519	-0.135720375106564	0.235955056179775	0.894736842105263	-0.967874794069193	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.152	9:9	150/150	Y:K	12.1451538387267	0.919026337424241	119.468044436244	0.101660271548247	5.01153666061564e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	SER	SRS797199	SRR1723718_SRR1723719	lineage2.2.1	cn	China:Anhui	pnca_p.tyr103ser	A	-	TYR	YA103	pnca_complex.pdb
245	S104G	L2	PZA	S	S104	104	G	1	Pre-XDR	DM	hydrophilic	hydrophobic	neutral	neutral	polar	non-polar	6.519	-0.7	-0.671	3.32695	-2.163	-0.68	81	91	-1.214	9	9	1	0.0064516129032258	loop	-0.833333333333333	5.37	0.0002156101768003	2.42493692178301	2.42493957425811	2.42473736947533	4.31445715363782e-25	4	0	0	0	4	0	2	2	1	0	1	0	2	5	15	-0.177304964539007	-0.173385012919897	0.12117476088841	-0.368797953964194	-0.178010471204189	0.852631578947368	-1	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	S	0.885806606247283	0.469406216824825	1.22442753211922	0.723445514749363	0.112662937620391	25.327054747104	0.384700446081433	0.430258985168156	0.366270051463612	0.0410854444106247	46.5881472867033	0.999999999999476	lineage2	dr_mutations_pyrazinamide	GLY	ERS457414,SAMEA2534012	ERR551539_ERR551540	lineage2.2.1	uz	Nukus	pnca_p.ser104gly	A	-	SER	SA104	pnca_complex.pdb
246	S104I	L4	PZA	S	S104	104	I	NA	MDR	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	6.519	-0.295	0.284	0.982352	-1.713	-0.39	82	91	-1.214	9	9	1	0.0064516129032258	loop	-0.833333333333333	5.37	NA	NA	NA	NA	NA	-18	2	0	2	2	0	0	2	-7	0	-11	0	-10	-6	-6	-0.0747213779128672	0.241496598639456	0.0357793981599516	-0.292071611253197	-0.102094240837696	0.863157894736842	-1	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ILE	ERR1555060	ERR1555060	lineage4.3.4.2			pnca_p.ser104ile	A	-	SER	SA104	pnca_complex.pdb
247	S104R	L4	PZA	S	S104	104	R	NA	XDR	DM	hydrophilic	hydrophilic	neutral	basic	polar	pos	6.519	-0.438	-0.525	-0.736092	-2.325	-0.47	85	91	-1.214	9	9	1	0.0064516129032258	loop	-0.833333333333333	5.37	0.0005749604714675	14.5802781289507	14.5802277940117	14.5801759034061	15.0663448164144	-68	-30	0	-30	4	0	0	4	-8	0	-4	-9	-2	-4	-2	-0.110942249240121	-0.135658914728682	-0.141523494578174	-0.396419437340153	-0.12303664921466	0.894736842105263	-1	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.229	-1.201	9:9	150/150	S	2.6796663501904	0.0010245352377103	0.816050632122416	3.28370108999367	3.35697961122126	99.5538692592611	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage4	dr_mutations_pyrazinamide	ARG	2370	ERR2432994	lineage4.3.4.1	br	Brazil	pnca_p.ser104arg	A	-	SER	SA104	pnca_complex.pdb
248	G105D	L4	PZA	G	G105	105	D	1	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	7.858	0.709	-1.651	2.15123	-2.29	-1.54	79	85	-0.506	7	7	0	0	helix	0.533333333333333	7.04	0.0001437401178668	19.411022297013	511754.463553489	Inf	4.73122070382274	-20	-32	0	-32	-2	0	2	-2	-5	0	0	-5	-18	-15	-25	0.317937219730942	-0.426614987080103	0.0783524792575703	-0.390451832907076	-0.403141361256544	0.831578947368421	-0.416803953871499	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	150/150	A:S:M:G:I:V:L	13.1456002256223	0.924803768447052	139.277183326685	0.0943844491368573	0.0001628371344425	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	ASP	ERR2510421	ERR2510421	lineage4.1.1.1	pk	Pakistan	pnca_p.gly105asp	A	G	GLY	GA105	pnca_complex.pdb
249	G105R	L2	PZA	G	G105	105	R	1	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	7.858	-0.634	-0.974	5.20362	-1.727	-0.66	81	91	-0.506	7	7	0	0	helix	0.533333333333333	7.04	7.18700589334483e-05	9.70142977291842	188179.926264685	Inf	0.763784339928386	-98	-50	-2	-48	-4	0	0	-4	-23	0	-8	-21	-22	-27	-39	-0.160587639311044	-0.251679586563308	0.189527167301627	-0.294458653026428	-0.172774869109948	0.852631578947368	-0.416803953871499	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	150/150	A:S:M:G:I:V:L	12.1451538387267	0.919026337424243	119.468044436248	0.101660271548245	5.01153666061226e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	ARG	ERR2516270	ERR2516270	lineage2.2.1	be	Belgium	pnca_p.gly105arg	A	G	GLY	GA105	pnca_complex.pdb
250	G105V	L2	PZA	G	G105	105	V	1	Pre-XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	7.858	-0.85	0.117	-2.03856	-1.183	-0.86	68	80	-0.506	7	7	0	0	helix	0.533333333333333	7.04	7.18700589334483e-05	9.70142977291842	188179.926264631	Inf	0.763784339928386	-2	0	0	0	-2	0	-2	2	-9	0	-1	-10	-8	-9	-21	-0.215298885511651	0.0994897959183673	-0.391940321464277	-0.201705029838022	-0.225130890052356	0.715789473684211	-0.416803953871499	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	150/150	A:S:M:G:I:V:L	12.1451538387264	0.919026337424233	119.468044436231	0.101660271548256	5.01153666062796e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	VAL	ERR2510727	ERR2510727	lineage2.2.1	it	Italy	pnca_p.gly105val	A	G	GLY	GA105	pnca_complex.pdb
251	F106C	L4	PZA	F	F106	106	C	0	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	10.466	-1.068	-1.594	3.977	-1.577	-2.12	59	75	-1.174	9	9	29	0.120833333333333	helix	-0.366666666666667	5.05	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	30	-2	0	-2	0	0	-2	-2	8	0	7	2	10	2	12	-0.270516717325228	-0.411886304909561	0.144850996874977	-0.268883205456095	-0.554973821989529	0.621052631578947	-0.967051070840198	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	150/150	F:L	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987749	NA	4692673.51081193	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	CYS	SAMN10961321	SRR8651642	lineage4.5	cn	China:Wenzhou	pnca_p.phe106cys	A	G	PHE	FA106	pnca_complex.pdb
252	F106L	L1	PZA	F	F106	106	L	0	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.466	-1.708	-1.425	1.92858	-0.947	-1.96	66	80	-1.174	9	9	29	0.120833333333333	helix	-0.366666666666667	5.05	0.0002156101768003	2.42493692178301	2.42493957425811	2.42473736947533	4.31445715363782e-25	10	4	0	4	0	0	0	0	2	0	0	2	6	2	4	-0.432624113475177	-0.368217054263566	0.0702430816075292	-0.161466325660699	-0.513089005235602	0.694736842105263	-0.967051070840198	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	150/150	F:L	0.885806606247283	0.469406216824826	1.22442753211922	0.723445514749363	0.112662937620387	25.3270547471038	0.384700446081433	0.430258985168156	0.366270051463612	0.0410854444106247	46.5881472867033	0.999999999999476	lineage1	other_mutations_pyrazinamide	LEU	ERR176618	ERR176618	lineage1.1.2	mw	Malawi	pnca_p.phe106leu	A	G	PHE	FA106	pnca_complex.pdb
253	F106S	L4	PZA	F	F106	106	S	1	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	10.466	-0.439	-2.455	4.86398	-1.914	-2.08	73	85	-1.174	9	9	29	0.120833333333333	helix	-0.366666666666667	5.05	7.18700589334483e-05	9.70142977291842	188179.926264447	Inf	0.763784339928386	32	2	0	2	-2	0	-2	-4	8	0	4	5	6	-2	6	-0.111195542046606	-0.634366925064599	0.177156739195361	-0.326342710997442	-0.544502617801047	0.768421052631579	-0.967051070840198	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	150/150	F:L	12.1451538387255	0.9190263374242	119.468044436172	0.101660271548299	5.01153666068353e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	SER	ERR2041728	ERR2041728	lineage4.1.2.1	rs	Serbia	pnca_p.phe106ser	A	G	PHE	FA106	pnca_complex.pdb
254	F106V	L2	PZA	F	F106	106	V	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.466	-1.625	-1.933	3.73767	-2.02	-2.1	69	80	-1.174	9	9	29	0.120833333333333	helix	-0.366666666666667	5.05	7.18700589334483e-05	9.70142977291842	188179.926264574	Inf	0.763784339928386	30	4	0	4	0	0	-2	0	6	0	4	3	10	2	8	-0.411600810536981	-0.499483204134367	0.136134077316997	-0.344416027280477	-0.549738219895288	0.726315789473684	-0.967051070840198	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	150/150	F:L	12.1451538387261	0.919026337424223	119.468044436213	0.101660271548269	5.01153666064512e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	VAL	ERR751650	ERR751650	lineage2.2.1	th	Thailand	pnca_p.phe106val	A	G	PHE	FA106	pnca_complex.pdb
255	E107D	L4	PZA	E	E107	107	D	NA	Other	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	8.13	-0.656	-0.224	0.965668	-0.917	-0.02	7	53	-0.068	5	5	88	0.394618834080717	helix	-0.366666666666667	3.69	NA	NA	NA	NA	NA	14	0	0	0	-2	0	-2	0	3	0	-1	4	-6	-4	-4	-0.166160081053698	-0.0578811369509043	0.0351717305633054	-0.156351236146633	-0.0052356020942408	0.0736842105263158	-0.0560131795716639	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	150/150	T:S:G:A:M:D:Q:F:H:Y:E:R:V:L	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ASP	SRR5065629	SRR5065629	lineage4.1.2.1	vn	Vietnam	pnca_p.glu107asp	A	G	GLU	EA107	pnca_complex.pdb
256	G108E	L2	PZA	G	G108	108	E	NA	XDR	OM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	10.939	0.303	-1.423	3.72313	-3.566	-0.78	39	66	-0.947	9	9	0	0	loop	0.1	5.18	NA	NA	NA	NA	NA	-44	-66	0	-66	-2	0	-2	0	-13	0	-8	-9	-12	-15	-23	0.135874439461883	-0.367700258397933	0.13560449886727	-0.608013640238704	-0.204188481675393	0.410526315789474	-0.78006589785832	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.062	-0.872	9:8	136/150	N:V:D:G:E:A:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLU	ERR067605,0224V-sc-2011-09-07T13:19:04Z-1276452	ERR067605	lineage2.2.1	ru	Russia	pnca_p.gly108glu	A	-	GLY	GA108	pnca_complex.pdb
257	G108R		PZA	G	G108	108	R	NA	MDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	10.939	-1.199	-0.972	8.65723	-2.025	-0.81	53	75	-0.947	9	9	0	0	loop	0.1	5.18	0.0002874802357337	4.85191417753471	4.85191414039983	4.85118443551178	1.17497090855698	-90	-46	0	-46	-4	0	-2	0	-22	0	-10	-18	-18	-17	-31	-0.303698074974671	-0.251162790697674	0.315315161095288	-0.345268542199488	-0.212041884816754	0.557894736842105	-0.78006589785832	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.062	-0.872	9:8	136/150	N:V:D:G:E:A:P	1.57937329521209	0.114342297004303	1.0002536454007	1.57897279602454	0.581889091312071	40.456291107579	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561		dr_mutations_pyrazinamide	ARG	SRR5818423	SRR5818423		dj	Djibouti	pnca_p.gly108arg	A	-	GLY	GA108	pnca_complex.pdb
258	D110H	L1	PZA	D	D110	110	H	NA	Sensitive	OM	hydrophilic	hydrophobic	acidic	basic	neg	pos	16.281	-1.3	-0.761	3.05477	-0.614	-0.26	-18	57	0.526	3	3	17	0.0880829015544041	loop	-0.933333333333333	4.81	NA	NA	NA	NA	NA	-10	20	0	20	-2	0	0	0	-3	0	-3	-1	0	2	0	-0.329280648429585	-0.196640826873385	0.111261372824685	-0.104688832054561	-0.0680628272251308	-0.193548387096774	0.168589743589744	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.225	0.59	4:3	128/150	Q:H:E:K:R:L:N:T:A:G:S:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	HIS	ERR3256124	ERR3256124	lineage1.2.1	ph	Philippines	pnca_p.asp110his	A	-	ASP	DA110	pnca_complex.pdb
259	E111D	L3;L4	PZA	E	E111	111	D	NA	Pre-MDR	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	18.382	0.103	0.156	0.183196	-0.053	-0.32	-55	78	1.616	1	1	167	0.748878923766816	loop	-3.5	3.55	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	10	0	0	0	0	0	0	0	1	0	1	1	0	0	0	0.0461883408071748	0.13265306122449	0.00667239708913963	-0.00903665814151748	-0.0837696335078534	-0.591397849462366	0.517948717948718	Stabilising	Stabilising	Destabilising	Destabilising	Destabilising	neutral	1.183	1.712	1:1	132/150	T:M:S:G:A:P:D:F:E:Q:L:V:N:R:K	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987759	NA	4692673.51081035	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3;lineage4	other_mutations_pyrazinamide	ASP	SRR6325444	SRR6325444	lineage4.3;lineage3	us		pnca_p.glu111asp	A	S	GLU	EA111	pnca_complex.pdb
260	N112Y	L1	PZA	N	N112	112	Y	NA	Sensitive	OM	hydrophilic	hydrophobic	neutral	neutral	polar	polar	19.93	-0.261	-0.378	4.97901	-0.297	-0.25	26	63	0.77	2	2	109	0.558974358974359	loop	-2.46666666666667	3.54	NA	NA	NA	NA	NA	-10	2	0	2	2	0	2	4	0	0	2	1	4	0	4	-0.0661094224924012	-0.0976744186046511	0.181346382185185	-0.0506393861892583	-0.0654450261780104	0.273684210526316	0.246794871794872	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.389	0.842	4:2	131/150	K:R:N:V:Q:E:H:D:P:S:M:G:A:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	TYR	SRR6152963	SRR6152963	lineage1.2.1	ca	Canada	pnca_p.asn112tyr	A	S	ASN	NA112	pnca_complex.pdb
261	T114M	L2	PZA	T	T114	114	M	NA	Sensitive	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	17.491	-1.152	0.246	-0.0405939	-0.077	0.38	25	63	-0.224	6	6	29	0.168604651162791	loop	-0.9	4.57	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-8	0	0	0	-2	0	-2	2	-6	0	0	-6	0	0	2	-0.291793313069909	0.209183673469388	-0.00780471814196724	-0.0131287297527707	0.213483146067416	0.263157894736842	-0.184514003294893	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-0.411	-0.147	7:6	148/150	S:A:P:D:H:K:T:W:E:Q:V:N:L:I:R:C	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987751	NA	4692673.51081327	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	MET	ERR1034985	ERR1034985	lineage2.2.1.1	za	South Africa	pnca_p.thr114met	A	-	THR	TA114	pnca_complex.pdb
262	T114P	L2	PZA	T	T114	114	P	1	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	17.491	-1.322	-0.651	5.39158	-2.268	-0.1	34	66	-0.224	6	6	29	0.168604651162791	loop	-0.9	4.57	7.18700589334483e-05	9.70142977291842	188179.926264667	Inf	0.763784339928386	4	0	0	0	0	0	0	4	-2	2	-4	0	4	-2	6	-0.334853090172239	-0.168217054263566	0.196373079640732	-0.386700767263427	-0.0261780104712041	0.357894736842105	-0.184514003294893	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.147	7:6	148/150	S:A:P:D:H:K:T:W:E:Q:V:N:L:I:R:C	12.1451538387266	0.91902633742424	119.468044436242	0.101660271548248	5.01153666061746e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	PRO	ERS456781	ERR550668_ERR550667_ERR550669	lineage2.2.1	uz	Nukus	pnca_p.thr114pro	A	-	THR	TA114	pnca_complex.pdb
263	P115L	L2	PZA	P	P115	115	L	0	Sensitive	OM	hydrophilic	hydrophobic	non-polar	non-polar	non-polar	non-polar	16.985	-0.972	-0.448	0.979724	-0.8	-0.55	8	53	0.774	2	2	50	0.314465408805031	strand	0.5	4.3	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-14	0	0	0	-2	0	-2	0	-8	0	-4	-5	-2	-2	0	-0.246200607902736	-0.115762273901809	0.0356836806794921	-0.136402387041773	-0.143979057591623	0.0842105263157895	0.248076923076923	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.389	0.842	4:2	147/150	T:G:A:S:P:D:E:Q:L:N:K:R	-8.98740878149641	0.940032862253632	119.46804443549	-0.0752285585987761	NA	4692673.51081015	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	LEU	non-MDR_11204	SRR6824507	lineage2.2.1	cn	China: Jianggan District, Hangzhou City, Zhejiang Province	pnca_p.pro115leu	A	B	PRO	PA115	pnca_complex.pdb
264	L116P	L4	PZA	L	L116	116	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	13.071	-1.504	-1.386	3.74372	-2.595	0.06	89	91	-0.924	8	8	0	0	helix	2	6.86	0.0002156101768003	9.70466975178797	9.70465898653047	9.70194990589383	2.29168245016684	18	0	0	0	2	2	-2	0	-3	2	-3	-2	8	0	8	-0.380952380952381	-0.358139534883721	0.136354431486243	-0.442455242966752	0.0337078651685393	0.936842105263158	-0.761120263591433	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.028	-0.872	9:8	147/150	I:V:L:M:A:T:F	2.27260607808816	0.0634455902260904	1.22442760158496	1.8560559033024	0.929172866365899	208.881603004966	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage4	dr_mutations_pyrazinamide	PRO	ERR2510202	ERR2510202	lineage4.3.4.2	pe	Peru	pnca_p.leu116pro	A	H	LEU	LA116	pnca_complex.pdb
265	L116R	L2	PZA	L	L116	116	R	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	13.071	-0.78	-1.733	4.65552	-4.189	-1.89	85	91	-0.924	8	8	0	0	helix	2	6.86	7.18700589334483e-05	9.70142977291842	188179.926264668	Inf	0.763784339928386	-54	-52	-2	-50	-2	0	-2	-2	-6	0	1	-7	-4	-12	0	-0.197568389057751	-0.447803617571059	0.169564172233189	-0.714236999147485	-0.494764397905759	0.894736842105263	-0.761120263591433	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.028	-0.872	9:8	147/150	I:V:L:M:A:T:F	12.1451538387266	0.91902633742424	119.468044436243	0.101660271548248	5.01153666061721e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	ARG	SAMN02584643	SRR1166113_SRR1169084	lineage2.2.1	md	Moldova	pnca_p.leu116arg	A	H	LEU	LA116	pnca_complex.pdb
266	L116V	L2	PZA	L	L116	116	V	0	XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.071	-1.648	-1.084	1.87491	-1.706	0.62	48	71	-0.924	8	8	0	0	helix	2	6.86	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	20	0	0	0	0	0	-2	0	2	0	2	0	2	0	2	-0.41742654508612	-0.280103359173127	0.0682883033821633	-0.290878090366581	0.348314606741573	0.505263157894737	-0.761120263591433	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.028	-0.872	9:8	147/150	I:V:L:M:A:T:F	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987746	NA	4692673.510814	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	dr_mutations_pyrazinamide	VAL	ERS1831679	ERR2041934	lineage2.2.1		Germany_extendedCAO	pnca_p.leu116val	A	H	LEU	LA116	pnca_complex.pdb
267	L117R	L2	PZA	L	L117	117	R	0	MDR	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	17.11	-0.585	-0.678	0.0947913	-1.884	-0.23	9	53	0.447	3	3	50	0.248756218905473	helix	1.36666666666667	4.46	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-28	-26	-2	-24	-2	0	-2	-2	4	0	1	4	2	2	0	-0.148176291793313	-0.175193798449612	0.0034525054815376	-0.321227621483376	-0.0602094240837696	0.0947368421052632	0.143269230769231	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.083	0.59	5:3	147/150	D:P:G:S:A:T:K:V:L:E:H	-8.98740878149642	0.940032862253633	119.468044435491	-0.0752285585987753	NA	4692673.51081308	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	ARG	MDR_25142	SRR6824543	lineage2.2.1	cn	China: Tonghai County, Yuxi City, Yunnan Province	pnca_p.leu117arg	A	H	LEU	LA117	pnca_complex.pdb
268	N118E	L4	PZA	N	N118	118	E	NA	Pre-MDR	OM	hydrophilic	hydrophilic	neutral	acidic	polar	neg	19.738	-0.713	0.78	-2.32726	0.371	-0.19	-29	61	0.692	2	2	80	0.41025641025641	helix	-0.2	4.06	NA	NA	NA	NA	NA	-6	-14	0	-14	-2	0	0	-4	0	0	0	-2	-2	0	0	-0.180597771023303	0.663265306122449	-0.447446743059294	0.313344594594595	-0.0497382198952879	-0.311827956989247	0.221794871794872	Destabilising	Stabilising	Stabilising	Stabilising	Destabilising	neutral	0.389	0.842	4:2	146/150	N:R:K:E:Y:Q:D:A:M:S:G:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLU	SRR6325445	SRR6325445	lineage4.3	us		pnca_p.asn118glu	A	H	ASN	NA118	pnca_complex.pdb
269	W119C	L2	PZA	W	W119	119	C	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	19.722	-1.498	-1.743	4.0665	-2.343	-1.91	48	71	0.224	4	4	14	0.0491228070175438	helix	-0.2	5.7	7.18700589334483e-05	9.70142977291842	188179.926264578	Inf	0.763784339928386	42	0	0	0	-2	0	-2	0	3	0	-1	4	12	10	10	-0.379432624113475	-0.450387596899225	0.148110781692757	-0.399488491048593	-0.5	0.505263157894737	0.0717948717948718	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	146/150	T:G:S:M:A:W:Q:F:E:Y:I:R:L:V	12.1451538387261	0.919026337424224	119.468044436214	0.101660271548269	5.01153666064432e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	CYS	SRR5266547	SRR5266547	lineage2.2.1	ru	Russia	pnca_p.trp119cys	A	H	TRP	WA119	pnca_complex.pdb
270	W119G	L4	PZA	W	W119	119	G	1	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	19.722	-1.84	-2.983	5.88168	-3.318	-3.39	70	85	0.224	4	4	14	0.0491228070175438	helix	-0.2	5.7	0.0001437401178668	19.411022297013	511754.463552391	Inf	4.73122070382274	52	0	0	0	0	0	0	0	5	0	-3	6	18	15	31	-0.466058763931104	-0.770801033591731	0.214223588458541	-0.565728900255755	-0.887434554973822	0.736842105263158	0.0717948717948718	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	146/150	T:G:S:M:A:W:Q:F:E:Y:I:R:L:V	13.1456002256201	0.924803768446985	139.277183326538	0.0943844491369419	0.0001628371344459	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	other_mutations_pyrazinamide	GLY	ERR2516678	ERR2516678	lineage4	pe	Peru	pnca_p.trp119gly	A	H	TRP	WA119	pnca_complex.pdb
271	W119L	L2	PZA	W	W119	119	L	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	19.722	-2.11	-1.981	2.39227	-1.186	-1.92	32	66	0.224	4	4	14	0.0491228070175438	helix	-0.2	5.7	NA	NA	NA	NA	NA	22	0	0	0	0	0	0	0	1	0	1	1	12	6	8	-0.534447821681864	-0.511886304909561	0.0871316807377676	-0.202216538789429	-0.50261780104712	0.336842105263158	0.0717948717948718	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	146/150	T:G:S:M:A:W:Q:F:E:Y:I:R:L:V	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	LEU	ERR775375	ERR775375	lineage2.2.1	pe		pnca_p.trp119leu	A	H	TRP	WA119	pnca_complex.pdb
272	W119R	L2	PZA	W	W119	119	R	0	MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	19.722	-0.807	-1.874	3.61213	-2.916	-1.89	70	85	0.224	4	4	14	0.0491228070175438	helix	-0.2	5.7	0.0005030904125341	6.47410526315789	6.47410517670654	6.47281975099898	5.34879705543722	-4	-30	-2	-28	-2	0	0	-2	-2	0	-5	-1	0	2	0	-0.204407294832827	-0.484237726098191	0.131561637249689	-0.497186700767263	-0.494764397905759	0.736842105263158	0.0717948717948718	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	146/150	T:G:S:M:A:W:Q:F:E:Y:I:R:L:V	1.86781040138793	0.0145043045254536	0.764079088633846	2.44452495713176	1.4262375473788	32.8877353882417	0.811179756363585	0.0192724555107824	1.71506294819644	1.09429403734416	44.2397805449942	0.0207365726835135	lineage2	dr_mutations_pyrazinamide	ARG	ERR2516865	ERR2516865	lineage2.2.1	gb	UK	pnca_p.trp119arg	A	H	TRP	WA119	pnca_complex.pdb
273	W119S	L4	PZA	W	W119	119	S	1	MDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	19.722	-0.995	-3.136	4.6385	-2.258	-3.44	66	80	0.224	4	4	14	0.0491228070175438	helix	-0.2	5.7	0.0001437401178668	19.411022297013	511754.463554449	Inf	4.73122070382274	44	0	0	0	-2	0	-2	0	6	0	0	6	14	10	12	-0.252026342451874	-0.810335917312662	0.16894426678516	-0.384995737425405	-0.900523560209424	0.694736842105263	0.0717948717948718	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	146/150	T:G:S:M:A:W:Q:F:E:Y:I:R:L:V	13.1456002256241	0.924803768447112	139.277183326815	0.0943844491367826	0.0001628371344395	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	SER	ERR2516993	ERR2516993	lineage4.1.2.1	gb	UK	pnca_p.trp119ser	A	H	TRP	WA119	pnca_complex.pdb
274	L120P	L4	PZA	L	L120	120	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	17.55	-1.644	-2.181	7.59698	-4.748	-1.51	79	85	-1.142	9	9	0	0	helix	-0.533333333333334	8.07	0.0018686215322696	254.917127071823	3820001.12561286	Inf	120.509921037495	16	16	0	16	2	2	2	-2	0	4	-4	-2	8	2	4	-0.416413373860182	-0.563565891472868	0.276698548212035	-0.809548167092924	-0.395287958115183	0.831578947368421	-0.940691927512356	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.201	-1.124	9:9	147/150	F:V:L	15.1557612752458	0.885234541058746	105.002771503186	0.14433677376588	15316.7789340045	20462689242793896	2.40639901531589	1.01650823124183e-20	19.9928891002838	32.1173872780919	Inf	4.89215110948636e-28	lineage4	dr_mutations_pyrazinamide	PRO	ERR3148160	ERR3148160	lineage4.1.1.3	mx	Mexico	pnca_p.leu120pro	A	H	LEU	LA120	pnca_complex.pdb
275	L120R	L3	PZA	L	L120	120	R	1	Pre-XDR	OM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	17.55	-0.772	-2.51	5.6624	-4.716	-1.93	74	85	-1.142	9	9	0	0	helix	-0.533333333333334	8.07	0.0005030904125341	12.1451137320977	12.1450773794065	12.1416664151504	11.0019205274607	-44	-32	-2	-30	0	0	0	0	-8	0	0	-13	-8	-12	-4	-0.195542046605876	-0.648578811369509	0.206236933544096	-0.804092071611253	-0.505235602094241	0.778947368421053	-0.940691927512356	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.201	-1.124	9:9	147/150	F:V:L	2.4969239337312	0.0028293218857323	0.83629495944555	2.98569769616526	2.61610833083038	84.8371270839259	1.08440158608155	0.0022516005975125	2.64750864476206	1.98635523014012	127.719946645636	0.0009101752788745	lineage3	other_mutations_pyrazinamide	ARG	ERR3335799	ERR3335799	lineage3	pk	Pakistan	pnca_p.leu120arg	A	H	LEU	LA120	pnca_complex.pdb
276	R121P	L2	PZA	R	R121	121	P	NA	Pre-XDR	DM	hydrophilic	hydrophilic	basic	non-polar	pos	non-polar	22.533	-1.047	-0.46	4.90155	-1.076	-0.11	77	85	0.506	3	3	125	0.456204379562044	helix	-1.4	3.76	7.18700589334483e-05	9.70142977291842	188179.926264617	Inf	0.763784339928386	40	20	2	18	4	2	2	0	0	4	-3	-2	4	4	0	-0.265197568389058	-0.118863049095607	0.178525120375294	-0.183461210571185	-0.0287958115183246	0.810526315789474	0.162179487179487	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.225	0.59	4:3	147/150	D:T:A:S:G:L:N:C:R:K:I:H:E:Q	12.1451538387264	0.919026337424231	119.468044436227	0.101660271548259	5.01153666063191e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	PRO	ERR1213849	ERR1213849	lineage2.2.2	kr	South Korea	pnca_p.arg121pro	A	H	ARG	RA121	pnca_complex.pdb
277	R121Q	L1	PZA	R	R121	121	Q	0	Other	OM	hydrophilic	hydrophilic	basic	neutral	pos	polar	22.533	-0.674	-0.169	1.41722	-0.286	-0.53	1	53	0.506	3	3	125	0.456204379562044	helix	-1.4	3.76	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	22	18	2	16	0	0	0	0	-1	0	0	-1	-2	-2	2	-0.170719351570415	-0.0436692506459948	0.0516182373123347	-0.0487638533674339	-0.138743455497382	0.0105263157894737	0.162179487179487	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.225	0.59	4:3	147/150	D:T:A:S:G:L:N:C:R:K:I:H:E:Q	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987758	NA	4692673.51080988	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	other_mutations_pyrazinamide	GLN	SAMEA2297105,ERS389492,ERR400534	ERR400534	lineage1.2.1	gb	United Kingdom	pnca_p.arg121gln	A	H	ARG	RA121	pnca_complex.pdb
278	R121W	L2	PZA	R	R121	121	W	NA	Sensitive	OM	hydrophilic	hydrophobic	basic	non-polar	pos	non-polar	22.533	-0.426	-0.538	1.95975	-0.442	-0.5	70	85	0.506	3	3	125	0.456204379562044	helix	-1.4	3.76	NA	NA	NA	NA	NA	6	18	2	16	2	0	2	0	0	0	0	0	-4	-6	-2	-0.10790273556231	-0.139018087855297	0.0713783608563582	-0.0753623188405797	-0.130890052356021	0.736842105263158	0.162179487179487	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.225	0.59	4:3	147/150	D:T:A:S:G:L:N:C:R:K:I:H:E:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	TRP	ERR234139	ERR234139	lineage2.2.1	cn	China	pnca_p.arg121trp	A	H	ARG	RA121	pnca_complex.pdb
279	Q122R	L4	PZA	Q	Q122	122	R	NA	Sensitive	OM	hydrophilic	hydrophilic	neutral	basic	polar	pos	24.683	-0.114	0.113	-1.69455	-0.152	-0.16	-41	72	1.334	1	1	132	0.586666666666667	loop	-4.16666666666667	3.49	NA	NA	NA	NA	NA	-24	-1	0	-1	2	0	-2	0	2	0	3	-1	2	0	2	-0.0288753799392097	0.0960884353741496	-0.325799815427209	-0.0259164535379369	-0.0418848167539267	-0.440860215053763	0.427564102564103	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	neutral	0.842	1.712	2:1	147/150	D:T:S:M:A:G:K:R:N:Q:H:E:Y	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ARG	ERR2229385	ERR2229385	lineage4.8	dk		pnca_p.gln122arg	A	T	GLN	QA122	pnca_complex.pdb
280	R123G	L1	PZA	R	R123	123	G	NA	MDR	OM	hydrophilic	hydrophobic	basic	neutral	pos	non-polar	23.59	-1.129	-1.212	2.77078	-1.005	-0.9	51	75	0.659	3	3	98	0.357664233576642	loop	-2.8	3.81	NA	NA	NA	NA	NA	50	16	2	14	2	0	0	2	1	0	3	-3	6	11	17	-0.28596757852077	-0.313178294573643	0.100917838853721	-0.171355498721228	-0.235602094240838	0.536842105263158	0.211217948717949	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.389	0.842	4:2	147/150	Q:H:F:Y:E:K:I:C:R:N:V:L:T:A:M	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	GLY	ERR1213910	ERR1213910	lineage1.2.1	ph	Philippines	pnca_p.arg123gly	A	T	ARG	RA123	pnca_complex.pdb
281	R123P	L2	PZA	R	R123	123	P	1	Pre-XDR	DM	hydrophilic	hydrophilic	basic	non-polar	pos	non-polar	23.59	-0.955	-0.348	3.53353	-1.065	-0.23	54	75	0.659	3	3	98	0.357664233576642	loop	-2.8	3.81	0.0001437401178668	19.411022297013	511754.463554485	Inf	4.73122070382274	42	16	2	14	3	0	1	2	4	2	-2	0	6	2	4	-0.241894630192503	-0.089922480620155	0.12869885415832	-0.181585677749361	-0.0602094240837696	0.568421052631579	0.211217948717949	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.389	0.842	4:2	147/150	Q:H:F:Y:E:K:I:C:R:N:V:L:T:A:M	13.1456002256242	0.924803768447114	139.27718332682	0.0943844491367796	0.0001628371344393	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	PRO	ERS458430,SAMEA2535028	ERR552955_ERR552956	lineage2.2.1	uz	Nukus	pnca_p.arg123pro	A	T	ARG	RA123	pnca_complex.pdb
282	G124R	L4	PZA	G	G124	124	R	NA	Pre-MDR	OM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	26.025	-0.075	-0.757	-0.489243	-0.439	-0.47	28	63	1.071	1	1	36	0.346153846153846	loop	-0.233333333333333	3.58	NA	NA	NA	NA	NA	-20	-14	-2	-12	-2	0	0	-2	-2	0	-5	-2	4	-1	1	-0.0189969604863221	-0.195607235142119	-0.0940634853495347	-0.0748508098891731	-0.12303664921466	0.294736842105263	0.343269230769231	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	0.59	1.183	3:1	147/150	A:S:G:D:Q:E:R:K:N	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ARG	ERR3148213	ERR3148213	lineage4.3.4.1	mx	Mexico	pnca_p.gly124arg	A	T	GLY	GA124	pnca_complex.pdb
283	G124S	L1	PZA	G	G124	124	S	0	Sensitive	OM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	26.025	0.612	-1.24	1.1133	-0.85	-0.39	-22	61	1.071	1	1	36	0.346153846153846	loop	-0.233333333333333	3.58	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	0	0	0	-2	0	-2	0	-1	0	-3	0	0	-3	-9	0.274439461883408	-0.320413436692506	0.0405488093590425	-0.144927536231884	-0.102094240837696	-0.236559139784946	0.343269230769231	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.59	1.183	3:1	147/150	A:S:G:D:Q:E:R:K:N	-8.98740878149641	0.940032862253633	119.46804443549	-0.0752285585987759	NA	4692673.51081215	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	other_mutations_pyrazinamide	SER	ERR2513039	ERR2513039	lineage1.1.2	gb	UK	pnca_p.gly124ser	A	T	GLY	GA124	pnca_complex.pdb
284	V125A	L1	PZA	V	V125	125	A	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	21.367	-1.941	-2.449	2.08114	-2.189	-1.89	49	71	-0.095	5	5	0	0	loop	0.1	6.12	NA	NA	NA	NA	NA	4	0	0	0	0	0	0	0	0	0	-1	2	4	4	5	-0.491641337386018	-0.6328165374677	0.0757996488902163	-0.373231031543052	-0.494764397905759	0.515789473684211	-0.0782537067545305	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	I:L:V:Q:F:A:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	ALA	DRR185019	DRR185019	lineage1.1.1	vn	Vietnam	pnca_p.val125ala	A	-	VAL	VA125	pnca_complex.pdb
285	V125D	L4	PZA	V	V125	125	D	NA	Other	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	21.367	-0.358	-3.013	3.9883	-4.507	-2.67	74	85	-0.095	5	5	0	0	loop	0.1	6.12	0.0002156101768003	0.807902480033628	4.5966027178939e-05	0	0.000393645565148	-8	-32	-2	-30	-2	0	-2	0	-8	0	-6	-1	2	2	-2	-0.0906788247213779	-0.778552971576227	0.145262567472082	-0.76845694799659	-0.698952879581152	0.778947368421053	-0.0782537067545305	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	I:L:V:Q:F:A:E	-9.98760797391818	0.930014323968984	113.719344729606	-0.0878268160765965	NA	127.948735098941	-0.0926410587047397	1	0	0	11.7369754738005	0.984170607395186	lineage4	dr_mutations_pyrazinamide	ASP	ERR2229701	ERR2229701	lineage4.8	dk		pnca_p.val125asp	A	-	VAL	VA125	pnca_complex.pdb
286	V125F	L2	PZA	V	V125	125	F	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	21.367	-1.707	-1.739	4.56	-1.65	-1.72	48	71	-0.095	5	5	0	0	loop	0.1	6.12	0.0001437401178668	1.2119588062211	4.59700131334263e-05	0	1.01056822389159e-25	-20	-4	-2	-2	0	0	0	0	-11	0	-6	-10	-2	-6	-2	-0.432370820668693	-0.449354005167959	0.166085125911465	-0.281329923273657	-0.450261780104712	0.505263157894737	-0.0782537067545305	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	I:L:V:Q:F:A:E	-9.98752126245059	0.942832958100734	139.277183325398	-0.0717096729269461	NA	144471.706314848	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	other_mutations_pyrazinamide	PHE	SRR1710096	SRR1710096	lineage2.2.1	cn		pnca_p.val125phe	A	-	VAL	VA125	pnca_complex.pdb
287	V125G	L4	PZA	V	V125	125	G	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	21.367	-1.814	-3.005	3.83404	-3.24	-2.63	70	85	-0.095	5	5	0	0	loop	0.1	6.12	0.0031622825930717	67.409751924722	67.4093600119011	67.3855171102593	174.918303180059	8	0	0	0	0	0	0	0	-2	0	-1	0	8	13	21	-0.459473150962513	-0.776485788113695	0.139644082488946	-0.552429667519182	-0.68848167539267	0.736842105263158	-0.0782537067545305	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.332	-0.038	6:5	147/150	I:L:V:Q:F:A:E	4.21078388085649	1.83664466117051e-12	0.597583217500306	7.04635564979589	24.5112444006964	278.636893097378	1.82872272889042	2.05851007546474e-28	27.6864470029886	21.4729050424094	338.090551978209	6.23802610818127e-40	lineage4	dr_mutations_pyrazinamide	GLY	ERR275229	ERR275229	lineage4.3.4.2	pt	Portugal	pnca_p.val125gly	A	-	VAL	VA125	pnca_complex.pdb
288	D126E	L4	PZA	D	D126	126	E	NA	Pre-XDR	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	23.083	0.035	-0.157	-0.565957	-0.068	-0.55	-71	87	3.12	1	1	66	0.341968911917098	loop	-0.933333333333333	4.35	NA	NA	NA	NA	NA	-2	2	0	2	0	0	0	0	-1	0	0	-1	0	0	-2	0.0156950672645739	-0.0405684754521963	-0.108812773975236	-0.0115942028985507	-0.143979057591623	-0.763440860215054	1	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	1.712	3.125	1:1	147/150	D:T:M:S:A:G:L:N:V:K:R:H:E:Y:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLU	SAMN01925669	SRR847782_SRR924233	lineage4.3.3	za	South Africa	pnca_p.asp126glu	A	-	ASP	DA126	pnca_complex.pdb
289	E127D	L4	PZA	E	E127	127	D	NA	Pre-XDR	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	20.54	-1.005	-1.341	2.77841	-0.674	-1.29	-42	72	0.916	2	2	55	0.246636771300448	strand	-0.933333333333333	5.17	NA	NA	NA	NA	NA	6	-2	0	-2	2	0	0	2	-3	0	-2	-3	0	0	0	-0.254559270516717	-0.346511627906977	0.101195740062209	-0.114919011082694	-0.337696335078534	-0.451612903225806	0.293589743589744	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.59	1.183	3:1	147/150	D:T:S:G:A:R:K:N:L:Q:H:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ASP	SAMN02628491	SRR1180156_SRR1172164_SRR1172203_SRR1183110	lineage4.3	ir	Iran	pnca_p.glu127asp	A	E	GLU	EA127	pnca_complex.pdb
290	E127V	L2	PZA	E	E127	127	V	NA	Pre-XDR	OM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	20.54	-2.247	0.454	-0.86796	-0.221	-0.33	12	59	0.916	2	2	55	0.246636771300448	strand	-0.933333333333333	5.17	NA	NA	NA	NA	NA	14	24	4	20	2	0	0	2	-3	0	-3	0	-2	-2	-2	-0.569148936170213	0.386054421768707	-0.166876874567408	-0.0376811594202899	-0.0863874345549738	0.126315789473684	0.293589743589744	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	0.59	1.183	3:1	147/150	D:T:S:G:A:R:K:N:L:Q:H:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	VAL	SRS797287	SRR1723816	lineage2.2.1	cn	China:Guangdong	pnca_p.glu127val	A	E	GLU	EA127	pnca_complex.pdb
291	V128F	L2	PZA	V	V128	128	F	1	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	16.575	-2.082	-1.849	2.63855	-1.167	-1.73	58	75	-0.394	6	6	0	0	strand	-0.933333333333333	8.31	7.18700589334483e-05	9.70142977291842	188179.926264397	Inf	0.763784339928386	-34	0	0	0	0	0	0	0	-11	0	-3	-10	-4	-2	-8	-0.527355623100304	-0.477777777777778	0.0961017344240561	-0.198976982097187	-0.452879581151832	0.610526315789474	-0.324546952224053	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.332	7:6	147/150	V:L:I:A	12.1451538387252	0.919026337424191	119.468044436157	0.101660271548309	5.01153666069775e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	PHE	ERR552933,SAMEA2535011,ERS458413	ERR552933	lineage2.2.1	uz	Nukus	pnca_p.val128phe	A	E	VAL	VA128	pnca_complex.pdb
292	V128G		PZA	V	V128	128	G	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	16.575	-1.823	-3.564	4.66562	-4.952	-3.82	75	85	-0.394	6	6	0	0	strand	-0.933333333333333	8.31	0.0010061808250682	29.234474017744	29.2344625578696	29.200882251631	41.8296883276733	16	0	0	0	0	0	0	0	0	0	0	0	6	7	23	-0.461752786220871	-0.920930232558139	0.169932036218213	-0.844330775788576	-1	0.789473684210526	-0.324546952224053	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.553	-0.332	7:6	147/150	V:L:I:A	3.37534823794128	9.91219225447489e-06	0.763812419492775	4.41908006704415	7.9656644717311	187.959563815878	1.46589528448265	3.94377277607243e-08	7.40408811526751	6.49943487243223	267.206211303594	9.95798685333635e-11		dr_mutations_pyrazinamide	GLY	SRR5818412	SRR5818412		dj	Djibouti	pnca_p.val128gly	A	E	VAL	VA128	pnca_complex.pdb
293	D129G	L4	PZA	D	D129	129	G	0	MDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	14.781	-3.948	-0.65	4.68356	-2.685	-0.43	77	85	0.074	5	5	0	0	strand	1.63333333333333	7.77	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	34	36	4	32	4	0	0	4	4	0	0	4	4	11	19	-1	-0.167958656330749	0.170585450068838	-0.457800511508951	-0.112565445026178	0.810526315789474	0.0237179487179487	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	I:V:L:E:Y:H:F:D:W:S:A:T	-8.98740878149641	0.940032862253633	119.468044435492	-0.0752285585987749	NA	4692673.51081121	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	GLY	ERR2516980	ERR2516980	lineage4.8	gb	UK	pnca_p.asp129gly	A	E	ASP	DA129	pnca_complex.pdb
294	D129N	L4	PZA	D	D129	129	N	NA	Sensitive	OM	hydrophilic	hydrophilic	acidic	neutral	neg	polar	14.781	-3.245	-0.735	1.83782	-2.127	-0.87	72	85	0.074	5	5	0	0	strand	1.63333333333333	7.77	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-2	38	4	34	-2	0	-2	0	0	0	-1	0	0	2	0	-0.82193515704154	-0.189922480620155	0.0669374048470633	-0.362659846547315	-0.227748691099476	0.757894736842105	0.0237179487179487	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	I:V:L:E:Y:H:F:D:W:S:A:T	-8.98740878149641	0.940032862253634	119.468044435493	-0.0752285585987741	NA	4692673.51081231	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	ASN	ERR190405	ERR190405	lineage4.3.4.1	mw	Malawi	pnca_p.asp129asn	A	E	ASP	DA129	pnca_complex.pdb
295	D129Y	L2	PZA	D	D129	129	Y	NA	Pre-MDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	14.781	-2.832	0.187	3.80937	0.436	0.12	42	71	0.074	5	5	0	0	strand	1.63333333333333	7.77	7.18700589334483e-05	9.70142977291842	188179.926264589	Inf	0.763784339928386	-40	34	4	30	4	0	0	2	-8	0	-3	-5	8	4	2	-0.717325227963526	0.159013605442177	0.138745547388894	0.368243243243243	0.0674157303370786	0.442105263157895	0.0237179487179487	Destabilising	Stabilising	Destabilising	Stabilising	Stabilising	effect	-0.147	0.225	6:4	147/150	I:V:L:E:Y:H:F:D:W:S:A:T	12.1451538387262	0.919026337424226	119.468044436217	0.101660271548266	5.0115366606409e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	TYR	SRR5074094	SRR5074094	lineage2.2.1.1	vn	Vietnam	pnca_p.asp129tyr	A	E	ASP	DA129	pnca_complex.pdb
296	V130A	L2	PZA	V	V130	130	A	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.402	-1.377	-2.827	3.70939	-2.959	-2.32	51	75	0.064	5	5	0	0	strand	1.63333333333333	11.27	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	2	0	0	0	0	0	-2	0	3	0	3	0	4	4	3	-0.348784194528875	-0.730490956072351	0.135104058158932	-0.504518329070759	-0.607329842931937	0.536842105263158	0.0205128205128205	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	M:F:D:C:I:L:V	-8.98740878149641	0.940032862253633	119.468044435491	-0.0752285585987751	NA	4692673.51081327	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	dr_mutations_pyrazinamide	ALA	ERR029210	ERR029210	lineage2.2.1	nl	Netherlands	pnca_p.val130ala	A	E	VAL	VA130	pnca_complex.pdb
297	V130E	L2	PZA	V	V130	130	E	1	MDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	10.402	-0.292	-3.207	5.05456	-5.67	-1.74	75	85	0.064	5	5	0	0	strand	1.63333333333333	11.27	0.0001437401178668	19.411022297013	511754.46355254	Inf	4.73122070382274	-46	-56	-4	-52	0	0	0	0	-5	0	0	-5	-2	2	-4	-0.0739614994934143	-0.828682170542636	0.184098077637512	-0.966751918158568	-0.455497382198953	0.789473684210526	0.0205128205128205	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	M:F:D:C:I:L:V	13.1456002256204	0.924803768446994	139.277183326557	0.0943844491369309	0.0001628371344454	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	GLU	ETB0035	SRR6257089	lineage2.2.1	ru	Russia:Leningrad Region	pnca_p.val130glu	A	E	VAL	VA130	pnca_complex.pdb
298	V130G	L2	PZA	V	V130	130	G	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	10.402	-1.126	-3.429	5.77612	-4.805	-3.46	73	85	0.064	5	5	0	0	strand	1.63333333333333	11.27	0.0001437401178668	19.411022297013	511754.463552424	Inf	4.73122070382274	20	0	0	0	0	0	-2	0	3	0	3	0	8	9	23	-0.285207700101317	-0.886046511627907	0.210378863482397	-0.81926683716965	-0.905759162303665	0.768421052631579	0.0205128205128205	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	M:F:D:C:I:L:V	13.1456002256202	0.924803768446987	139.277183326542	0.0943844491369394	0.0001628371344458	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	GLY	SRR6397471	SRR6397471	lineage2.2.1	ca	Canada	pnca_p.val130gly	A	E	VAL	VA130	pnca_complex.pdb
299	V130M	L1	PZA	V	V130	130	M	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.402	-2.265	-1.539	-0.707302	-2.385	-1.31	34	66	0.064	5	5	0	0	strand	1.63333333333333	11.27	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-58	0	0	0	0	0	-2	0	-2	0	3	-5	-4	2	-6	-0.57370820668693	-0.397674418604651	-0.135988233484581	-0.406649616368286	-0.342931937172775	0.357894736842105	0.0205128205128205	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	147/150	M:F:D:C:I:L:V	-8.98740878149642	0.940032862253634	119.468044435493	-0.0752285585987741	NA	4692673.5108132	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	other_mutations_pyrazinamide	MET	ERR3256144	ERR3256144	lineage1.2.1	ph	Philippines	pnca_p.val130met	A	E	VAL	VA130	pnca_complex.pdb
300	V131F	L2	PZA	V	V131	131	F	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	9.063	-2.323	-1.59	1.54666	-1.786	-1.52	72	85	0.204	4	4	1	0.0057471264367816	strand	2.66666666666667	10.36	0.0001437401178668	19.411022297013	511754.463553315	Inf	4.73122070382274	-44	-4	0	-4	0	0	0	0	-15	0	-7	-8	-6	-8	-8	-0.588399189463019	-0.410852713178295	0.0563327238689093	-0.304518329070759	-0.397905759162304	0.757894736842105	0.0653846153846154	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	147/150	D:M:S:A:G:T:C:V:L	13.1456002256219	0.924803768447041	139.277183326661	0.0943844491368713	0.000162837134443	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	PHE	SAMN10961371	SRR8651611	lineage2.2.1	cn	China:Wenzhou	pnca_p.val131phe	A	E	VAL	VA131	pnca_complex.pdb
301	V131G	L4	PZA	V	V131	131	G	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	9.063	-1.774	-3.182	5.35345	-3.989	-2.53	54	75	0.204	4	4	1	0.0057471264367816	strand	2.66666666666667	10.36	0.0001437401178668	19.411022297013	511754.463553338	Inf	4.73122070382274	6	0	0	0	0	0	0	0	0	0	0	0	2	3	19	-0.449341438703141	-0.822222222222222	0.194984302041827	-0.680136402387042	-0.662303664921466	0.568421052631579	0.0653846153846154	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	147/150	D:M:S:A:G:T:C:V:L	13.145600225622	0.924803768447044	139.277183326667	0.0943844491368676	0.0001628371344429	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	other_mutations_pyrazinamide	GLY	ERR2510219	ERR2510219	lineage4.1.2.1	pe	Peru	pnca_p.val131gly	A	E	VAL	VA131	pnca_complex.pdb
302	G132A	L4	PZA	G	G132	132	A	NA	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	6.912	-1.92	-0.829	5.5504	-2.927	-0.28	80	91	-1.191	9	9	0	0	strand	2.76666666666667	8.75	0.0005749604714675	14.5802781289507	14.5802277940117	14.5801759034061	15.0663448164144	-4	0	0	0	0	0	0	0	-6	0	-6	-2	0	-7	-18	-0.486322188449848	-0.21421188630491	0.20215764974978	-0.499062233589088	-0.0732984293193717	0.842105263157895	-0.981054365733114	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	147/150	G	2.6796663501904	0.0010245352377104	0.816050632122417	3.28370108999367	3.35697961122016	99.5538692591549	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage4	dr_mutations_pyrazinamide	ALA	ERR3148191	ERR3148191	lineage4.1.2.1	mx	Mexico	pnca_p.gly132ala	A	E	GLY	GA132	pnca_complex.pdb
303	G132D	L2	PZA	G	G132	132	D	NA	MDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	6.912	-0.822	-2.518	18.6426	-3.507	-1.46	92	95	-1.191	9	9	0	0	strand	2.76666666666667	8.75	0.0004312203536006	58.3312262958281	1393450.94022859	Inf	23.547488635408	-22	-42	-2	-40	0	0	0	0	-11	0	-9	-6	-4	-9	-23	-0.208206686930091	-0.650645994832041	0.679004071999359	-0.597953964194373	-0.382198952879581	0.968421052631579	-0.981054365733114	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	147/150	G	14.1472939191334	0.915018435775164	132.576060676564	0.106710773022948	4.17896182038204	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage2	dr_mutations_pyrazinamide	ASP	SAMN02414914	SRR1146372_SRR1144743_SRR1166918_SRR1162871	lineage2.2.1	se	Sweden	pnca_p.gly132asp	A	E	GLY	GA132	pnca_complex.pdb
304	G132S	L4	PZA	G	G132	132	S	1	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	6.912	-0.312	-2.061	10.5344	-3.165	-1.17	85	91	-1.191	9	9	0	0	strand	2.76666666666667	8.75	0.0005749604714675	77.8405735976381	1394626.35224766	Inf	33.1802749271012	-4	0	0	0	0	0	0	0	-8	0	-8	-4	0	-7	-19	-0.0790273556231003	-0.532558139534884	0.383685778596872	-0.539641943734015	-0.306282722513089	0.894736842105263	-0.981054365733114	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.229	-1.178	9:9	147/150	G	14.1481370895088	0.901927875940771	114.814237034556	0.123226330243789	82.9586021393604	NA	1.89120602753485	7.2326389241213e-07	6.1407032156959	8.29603358299821	Inf	8.39983046099388e-09	lineage4	dr_mutations_pyrazinamide	SER	ERR2515345	ERR2515345	lineage4.1.1.3	za	South Africa	pnca_p.gly132ser	A	E	GLY	GA132	pnca_complex.pdb
305	I133S	L2	PZA	I	I133	133	S	NA	XDR	OM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	3.05	0.578	-3.219	3.30196	-2.461	-3.47	70	85	-0.739	8	8	3	0.015228426395939	strand	1.96666666666667	7.9	7.18700589334483e-05	9.70142977291842	188179.926264589	Inf	0.763784339928386	34	0	0	0	-2	0	-2	0	0	0	0	0	6	8	0	0.259192825112108	-0.831782945736434	0.120264570691803	-0.419607843137255	-0.908376963350785	0.736842105263158	-0.608731466227348	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	147/150	V:L:I:M:F	12.1451538387262	0.919026337424226	119.468044436218	0.101660271548266	5.01153666064073e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	SER	SAMN07236533,SRR5709884	SRR5709884	lineage2.2.1	th	Thailand	pnca_p.ile133ser	A	E	ILE	IA133	pnca_complex.pdb
306	I133T	L2	PZA	I	I133	133	T	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	3.05	0.703	-2.788	1.57844	-1.862	-2.56	68	80	-0.739	8	8	3	0.015228426395939	strand	1.96666666666667	7.9	0.0031622825930717	6.43697178374994	6.43697172567821	6.43569721125883	46.3588997045238	34	0	0	0	-2	0	-2	0	0	0	0	0	4	4	0	0.3152466367713	-0.720413436692506	0.0574902206455467	-0.317476555839727	-0.670157068062827	0.715789473684211	-0.608731466227348	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.674	8:7	147/150	V:L:I:M:F	1.86205820059201	1.05183177238756e-09	0.305188422646507	6.10133957390906	3.55222271070208	11.8555583489231	0.808681605417837	2.90039620895666e-09	8.53754267120101	3.39739251160169	12.3875045168727	9.84592753332283e-12	lineage2	dr_mutations_pyrazinamide	THR	ERS457609	ERR551811_ERR551810_ERR551809	lineage2.2.1	uz	Nukus	pnca_p.ile133thr	A	E	ILE	IA133	pnca_complex.pdb
307	A134D	L4	PZA	A	A134	134	D	0	Pre-XDR	OM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	3.047	0.582	-2.98	1.03055	-3.856	-1.13	85	91	-1.111	9	9	10	0.0775193798449612	loop	1.86666666666667	6.77	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-24	-34	0	-34	-2	0	-2	0	0	0	0	-2	-4	-2	-3	0.260986547085202	-0.770025839793282	0.0375348742342237	-0.657459505541347	-0.295811518324607	0.894736842105263	-0.915156507413509	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.178	-1.094	9:9	147/150	V:T:S:A	-8.98740878149643	0.940032862253634	119.468044435494	-0.0752285585987736	NA	4692673.510814	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	ASP	SRR2100459,SAMN03647632	SRR2100459	lineage4.4.1.1			pnca_p.ala134asp	A	-	ALA	AA134	pnca_complex.pdb
308	A134G	L4	PZA	A	A134	134	G	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	3.047	-0.378	-1.618	-1.28536	-2.508	-1.88	79	85	-1.111	9	9	10	0.0775193798449612	loop	1.86666666666667	6.77	NA	NA	NA	NA	NA	2	0	0	0	0	0	0	0	0	0	1	0	0	3	14	-0.0957446808510638	-0.418087855297158	-0.247127585941706	-0.427621483375959	-0.492146596858639	0.831578947368421	-0.915156507413509	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-1.094	9:9	147/150	V:T:S:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLY	ERR3148178	ERR3148178	lineage4.3.3	mx	Mexico	pnca_p.ala134gly	A	-	ALA	AA134	pnca_complex.pdb
309	A134P	L2	PZA	A	A134	134	P	1	MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	3.047	0.077	-1.426	-5.2012	-2.503	-0.19	88	91	-1.111	9	9	10	0.0775193798449612	loop	1.86666666666667	6.77	7.18700589334483e-05	9.70142977291842	188179.92626465	Inf	0.763784339928386	0	0	0	0	0	0	0	0	1	1	-1	-2	-2	0	-9	0.0345291479820627	-0.368475452196382	-1	-0.426768968456948	-0.0497382198952879	0.926315789473684	-0.915156507413509	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-1.094	9:9	147/150	V:T:S:A	12.1451538387265	0.919026337424237	119.468044436237	0.101660271548252	5.01153666062244e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	PRO	SAMEA2535049,ERS458451	ERR552982_ERR552983	lineage2.2.1	uz	Nukus	pnca_p.ala134pro	A	-	ALA	AA134	pnca_complex.pdb
310	A134T	L1	PZA	A	A134	134	T	NA	XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	3.047	0.875	-1.925	-0.943073	-1.723	-1.44	61	80	-1.111	9	9	10	0.0775193798449612	loop	1.86666666666667	6.77	NA	NA	NA	NA	NA	-6	0	0	0	-2	0	-2	0	-2	0	0	-4	-2	-2	-3	0.39237668161435	-0.497416020671835	-0.181318349611628	-0.293776641091219	-0.37696335078534	0.642105263157895	-0.915156507413509	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-1.094	9:9	147/150	V:T:S:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	THR	SRR7898227	SRR7898227	lineage1.1.2	in	India	pnca_p.ala134thr	A	-	ALA	AA134	pnca_complex.pdb
311	A134V	L4	PZA	A	A134	134	V	0	Other	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	3.047	0.118	-0.414	-1.46184	-1.64	-0.88	74	85	-1.111	9	9	10	0.0775193798449612	loop	1.86666666666667	6.77	0.0003593502946672	19.4256842105263	19.4256680571767	19.4169790983636	9.87561363723508	-6	0	0	0	0	0	0	0	-2	0	-2	-6	-2	-2	-3	0.052914798206278	-0.106976744186046	-0.281058217334461	-0.279624893435635	-0.230366492146597	0.778947368421053	-0.915156507413509	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.178	-1.094	9:9	147/150	V:T:S:A	2.96659528728018	0.0079500503166053	1.11770528264822	2.65418382943607	2.87215213651462	380.097158975588	1.28837632444196	0.0036816889015929	2.43395291165754	1.92061868691892	951.403114304703	0.0016748373055023	lineage4	dr_mutations_pyrazinamide	VAL	ERR2513980	ERR2513980	lineage4.8	gb	UK	pnca_p.ala134val	A	-	ALA	AA134	pnca_complex.pdb
312	T135A	L2	PZA	T	T135	135	A	NA	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	6.021	-1.324	-0.347	0.939971	-1.151	-0.32	72	85	-0.612	7	7	3	0.0174418604651162	loop	-0.8	5.97	NA	NA	NA	NA	NA	4	0	0	0	4	0	-2	4	4	0	4	2	6	6	3	-0.335359675785208	-0.0896640826873385	0.0342357898877469	-0.196248934356351	-0.0837696335078534	0.757894736842105	-0.504118616144975	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.553	8:7	147/150	G:S:M:A:T:L:R:E:Y:F:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	ALA	SAMN02584663	SRR1166176_SRR1169028	lineage2.2.1	md	Moldova	pnca_p.thr135ala	A	T	THR	TA135	pnca_complex.pdb
313	T135I	L4	PZA	T	T135	135	I	0	MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	6.021	-1.039	0.442	-0.624872	-0.279	0.66	86	91	-0.612	7	7	3	0.0174418604651162	loop	-0.8	5.97	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-10	0	0	0	4	0	-2	4	-3	0	-1	0	2	2	-4	-0.263171225937183	0.375850340136054	-0.120139967699762	-0.0475703324808184	0.370786516853933	0.905263157894737	-0.504118616144975	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-0.728	-0.553	8:7	147/150	G:S:M:A:T:L:R:E:Y:F:Q	-8.9874087814964	0.940032862253633	119.46804443549	-0.0752285585987755	NA	4692673.5108109	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	ILE	ERR2516192	ERR2516192	lineage4.2.2	be	Belgium	pnca_p.thr135ile	A	T	THR	TA135	pnca_complex.pdb
314	T135P	L4	PZA	T	T135	135	P	1	MDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	6.021	-1.103	-0.141	4.22994	-2.513	0.5	93	95	-0.612	7	7	3	0.0174418604651162	loop	-0.8	5.97	0.0019404915912031	127.447513812155	127.447463030391	127.547977311481	114.177692609973	4	0	0	0	6	2	-2	4	0	2	0	0	4	4	-2	-0.279381965552178	-0.0364341085271317	0.154063622258321	-0.42847399829497	0.280898876404494	0.978947368421053	-0.504118616144975	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.728	-0.553	8:7	147/150	G:S:M:A:T:L:R:E:Y:F:Q	4.84770422501703	1.95563371409724e-06	1.01886282652061	4.75795573146171	27.0895053611136	2275.86435679243	2.10533136786968	2.29679039420522e-19	18.638878636844	20.9103539725502	5063.91643152716	1.19139212303841e-26	lineage4	dr_mutations_pyrazinamide	PRO	SAMN03648845,SRR2101598	SRR2101598	lineage4.1.2.1			pnca_p.thr135pro	A	T	THR	TA135	pnca_complex.pdb
315	T135S	L4	PZA	T	T135	135	S	0	MDR	OM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	6.021	-0.709	-1.605	0.77889	-1.567	-0.71	65	80	-0.612	7	7	3	0.0174418604651162	loop	-0.8	5.97	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	0	0	0	0	0	0	0	0	3	0	1	2	2	2	2	-0.179584599797366	-0.414728682170543	0.0283688692371011	-0.267178175618073	-0.18586387434555	0.684210526315789	-0.504118616144975	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.553	8:7	147/150	G:S:M:A:T:L:R:E:Y:F:Q	-8.98740878149641	0.940032862253632	119.46804443549	-0.075228558598776	NA	4692673.51081184	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	SER	0600D-sc-2011-10-12T14:59:43Z-1289186,ERR117452	ERR117452	lineage4.3.3	ru	Russia,Samara	pnca_p.thr135ser	A	T	THR	TA135	pnca_complex.pdb
316	D136A	L2	PZA	D	D136	136	A	1	MDR	OM	hydrophilic	hydrophobic	acidic	non-polar	neg	non-polar	6.179	-2.506	0.66	1.50112	-0.993	1.78	73	85	-1.076	9	9	6	0.0310880829015544	loop	-2.46666666666667	5.37	7.18700589334483e-05	9.70142977291842	188179.926264403	Inf	0.763784339928386	4	29	0	29	8	0	2	2	4	0	0	4	6	10	-3	-0.634751773049645	0.561224489795918	0.0546740579403988	-0.169309462915601	1	0.768421052631579	-0.886326194398682	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.152	-1.028	9:9	147/150	S:E:D	12.1451538387252	0.919026337424193	119.468044436159	0.101660271548308	5.01153666069587e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ALA	ERR551545	ERR551545	lineage2.2.1	sz	Swaziland	pnca_p.asp136ala	A	T	ASP	DA136	pnca_complex.pdb
317	D136G	L2	PZA	D	D136	136	G	0	MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	6.179	-2.578	0.314	2.04991	-1.455	0.52	79	85	-1.076	9	9	6	0.0310880829015544	loop	-2.46666666666667	5.37	0.0008624407072013	3.46852810205801	3.46852813512699	3.46809703626328	3.5269411605584	6	29	0	29	8	0	2	2	5	0	5	2	8	13	15	-0.652988855116515	0.267006802721088	0.0746621843107832	-0.248081841432225	0.292134831460674	0.831578947368421	-0.886326194398682	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.152	-1.028	9:9	147/150	S:E:D	1.24373033542254	0.0337889223820885	0.585951699076682	2.12258166907334	1.02578380758221	10.8758695145359	0.540145217509122	0.0400825565026472	1.39704458655237	0.867177455332643	12.7072994899286	0.0603790832350862	lineage2	dr_mutations_pyrazinamide	GLY	ERR2516443	ERR2516443	lineage2.2.1	pe	Peru	pnca_p.asp136gly	A	T	ASP	DA136	pnca_complex.pdb
318	D136N	L2	PZA	D	D136	136	N	NA	Pre-MDR	DM	hydrophilic	hydrophilic	acidic	neutral	neg	polar	6.179	-1.58	0.216	0.961088	-1.377	0.48	79	85	-1.076	9	9	6	0.0310880829015544	loop	-2.46666666666667	5.37	0.0005030904125341	3.63983585858586	3.63983585304829	3.63936365398778	1.71237896478786	0	29	0	29	4	0	0	2	-5	0	3	-9	4	2	-4	-0.400202634245187	0.183673469387755	0.0350049169938592	-0.234782608695652	0.269662921348315	0.831578947368421	-0.886326194398682	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-1.152	-1.028	9:9	147/150	S:E:D	1.29193858531542	0.0908665432562708	0.764078977125449	1.69084430273928	0.716519099632433	16.5222710930887	0.561081799221107	0.101883776579733	0.99189496518722	0.532738756693438	21.52594647947	0.190677002690395	lineage2	dr_mutations_pyrazinamide	ASN	SRR5073892	SRR5073892	lineage2.2.1.1	vn	Vietnam	pnca_p.asp136asn	A	T	ASP	DA136	pnca_complex.pdb
319	D136Y	L4	PZA	D	D136	136	Y	NA	MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	6.179	-1.662	-0.96	6.12929	-1.07	-0.23	86	91	-1.076	9	9	6	0.0310880829015544	loop	-2.46666666666667	5.37	0.0002156101768003	29.1287878787879	511969.848429002	Inf	9.2872640727157	-32	25	0	25	6	0	2	4	-6	0	-4	-4	-2	2	-2	-0.4209726443769	-0.248062015503876	0.223242083639887	-0.182438192668372	-0.0602094240837696	0.905263157894737	-0.886326194398682	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.152	-1.028	9:9	147/150	S:E:D	13.1460210125005	0.907969111549614	113.719344731947	0.115600569485232	0.183926936277317	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage4	dr_mutations_pyrazinamide	TYR	ERR2510215	ERR2510215	lineage4.3.4.1	pe	Peru	pnca_p.asp136tyr	A	T	ASP	DA136	pnca_complex.pdb
320	H137N	L3	PZA	H	H137	137	N	0	MDR	OM	hydrophobic	hydrophilic	basic	neutral	pos	polar	3.419	-0.124	0.189	0.40144	-0.475	0.6	65	80	-0.415	7	7	84	0.375	loop	-1.4	4.6	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	14	3	0	3	0	0	0	0	-1	0	1	-1	4	2	2	-0.0314083080040526	0.160714285714286	0.0146213186284865	-0.0809889173060528	0.337078651685393	0.684210526315789	-0.341845140032949	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.615	-0.332	7:6	147/150	Q:H:F:E:Y:I:V:W	-8.98740878149642	0.940032862253634	119.468044435493	-0.075228558598774	NA	4692673.51081254	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage3	other_mutations_pyrazinamide	ASN	ERR2516643	ERR2516643	lineage3	nl	Netherlands	pnca_p.his137asn	A	T	HIS	HA137	pnca_complex.pdb
321	H137P	L4	PZA	H	H137	137	P	NA	Pre-XDR	DM	hydrophobic	hydrophilic	basic	non-polar	pos	non-polar	3.419	-0.765	0.366	2.19045	-0.882	1.03	86	91	-0.415	7	7	84	0.375	loop	-1.4	4.6	NA	NA	NA	NA	NA	34	3	0	3	2	0	2	0	0	4	-2	-4	2	-4	0	-0.193768996960486	0.311224489795918	0.0797809570291159	-0.150383631713555	0.578651685393258	0.905263157894737	-0.341845140032949	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.615	-0.332	7:6	147/150	Q:H:F:E:Y:I:V:W	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	PRO	ERR2707085	ERR2707085	lineage4.1.2	cd	Democratic Republic of Congo	pnca_p.his137pro	A	T	HIS	HA137	pnca_complex.pdb
322	H137R	L2	PZA	H	H137	137	R	1	MDR	DM	hydrophobic	hydrophilic	basic	basic	pos	pos	3.419	0.466	-0.272	0.488665	-0.317	0.21	76	85	-0.415	7	7	84	0.375	loop	-1.4	4.6	0.0002874802357337	4.85191417753471	4.85191414039981	4.85118443551178	1.17497090855698	-52	-2	0	-2	0	0	0	0	1	0	4	-1	-2	-2	-4	0.208968609865471	-0.0702842377260982	0.0177982429941943	-0.0540494458653026	0.117977528089888	0.8	-0.341845140032949	Stabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.615	-0.332	7:6	147/150	Q:H:F:E:Y:I:V:W	1.57937329521209	0.114342297004304	1.0002536454007	1.57897279602454	0.581889091312819	40.4562911075324	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561	lineage2	dr_mutations_pyrazinamide	ARG	MDR_10142	SRR6824295	lineage2.2.1	cn	China: Huqiu Hi-tech District, Suzhou City, Jiangsu Province	pnca_p.his137arg	A	T	HIS	HA137	pnca_complex.pdb
323	H137Y	L1	PZA	H	H137	137	Y	NA	Sensitive	OM	hydrophobic	hydrophobic	basic	neutral	pos	polar	3.419	-0.005	0.859	0.344473	-0.394	1.72	-7	53	-0.415	7	7	84	0.375	loop	-1.4	4.6	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-42	3	0	3	2	0	2	0	-1	0	1	-4	-2	-2	0	-0.0012664640324214	0.730442176870748	0.0125464564864255	-0.0671781756180733	0.966292134831461	-0.0752688172043011	-0.341845140032949	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	neutral	-0.615	-0.332	7:6	147/150	Q:H:F:E:Y:I:V:W	-8.98740878149642	0.940032862253633	119.468044435491	-0.0752285585987755	NA	4692673.51081285	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage1	other_mutations_pyrazinamide	TYR	ERR767966	ERR767966	lineage1.1.1	th	Thailand	pnca_p.his137tyr	A	T	HIS	HA137	pnca_complex.pdb
324	C138G	L4	PZA	C	C138	138	G	NA	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	neutral	non-polar	non-polar	3.282	-0.012	-0.015	1.12063	-2.578	1.06	90	95	-1.148	9	9	12	0.0718562874251497	helix	1.16666666666667	6.7	NA	NA	NA	NA	NA	16	0	0	0	2	0	0	0	3	0	3	0	6	9	19	-0.0030395136778115	-0.003875968992248	0.0408157839145099	-0.439556692242114	0.595505617977528	0.947368421052632	-0.945634266886326	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-1.221	-1.124	9:9	147/150	C:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLY	ERR1034711	ERR1034711	lineage4.1.2.1	pe	Peru	pnca_p.cys138gly	A	H	CYS	CA138	pnca_complex.pdb
325	C138R	L2	PZA	C	C138	138	R	1	MDR	DM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	3.282	0.35	0.099	-2.11829	-2.383	0.53	91	95	-1.148	9	9	12	0.0718562874251497	helix	1.16666666666667	6.7	0.0008624407072013	116.958174904943	3797407.11818115	Inf	52.5277145499225	-86	-32	0	-32	-2	0	-2	-2	-5	0	-1	-6	-14	-6	-12	0.15695067264574	0.0841836734693877	-0.407269476274706	-0.406308610400682	0.297752808988764	0.957894736842105	-0.945634266886326	Stabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	-1.221	-1.124	9:9	147/150	C:S	15.1498290544778	0.921917088417709	154.559892765108	0.0980191483278375	60.4370486597206	NA	2.06803058263964	6.09960824712391e-10	9.21469805705084	13.5248372165465	Inf	4.24219331644313e-13	lineage2	dr_mutations_pyrazinamide	ARG	ERR2041744	ERR2041744	lineage2.2.1	rs	Serbia	pnca_p.cys138arg	A	H	CYS	CA138	pnca_complex.pdb
326	C138S	L2	PZA	C	C138	138	S	NA	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	3.282	0.814	-0.002	-0.229289	-1.639	1.02	87	91	-1.148	9	9	12	0.0718562874251497	helix	1.16666666666667	6.7	NA	NA	NA	NA	NA	4	0	0	0	0	0	-2	0	3	0	3	0	2	0	2	0.365022421524664	-0.000516795865633	-0.0440838652618626	-0.279454390451833	0.573033707865168	0.91578947368421	-0.945634266886326	Stabilising	Destabilising	Stabilising	Destabilising	Stabilising	effect	-1.221	-1.124	9:9	147/150	C:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	dr_mutations_pyrazinamide	SER	SRR7517719	SRR7517719	lineage2.2.1	ch	Switzerland	pnca_p.cys138ser	A	H	CYS	CA138	pnca_complex.pdb
327	C138W	L2	PZA	C	C138	138	W	NA	Pre-MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	3.282	0.943	-1.048	-1.71951	-1.165	-0.99	91	95	-1.148	9	9	12	0.0718562874251497	helix	1.16666666666667	6.7	NA	NA	NA	NA	NA	-72	0	0	0	0	0	-2	0	-2	0	0	-3	-12	-2	-16	0.422869955156951	-0.270801033591731	-0.330598707990464	-0.198635976129582	-0.259162303664921	0.957894736842105	-0.945634266886326	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	147/150	C:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	TRP	SRS797297	SRR1723826_SRR1723827	lineage2.2.1	cn	China:Fujian	pnca_p.cys138trp	A	H	CYS	CA138	pnca_complex.pdb
328	C138Y	L7	PZA	C	C138	138	Y	NA	Other	DM	hydrophobic	hydrophobic	neutral	neutral	non-polar	polar	3.282	0.914	-0.518	-0.565013	-1.271	-0.44	90	95	-1.148	9	9	12	0.0718562874251497	helix	1.16666666666667	6.7	NA	NA	NA	NA	NA	-72	-40	0	-40	0	0	-2	0	1	0	0	1	-12	-2	-14	0.409865470852018	-0.133850129198966	-0.108631277397524	-0.216709292412617	-0.115183246073298	0.947368421052632	-0.945634266886326	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.124	9:9	147/150	C:S	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage7	dr_mutations_pyrazinamide	TYR	ERR233349	ERR233349	lineage7			pnca_p.cys138tyr	A	H	CYS	CA138	pnca_complex.pdb
329	V139A	L4	PZA	V	V139	139	A	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.113	-0.537	-1.598	2.24246	-2.438	-1.84	52	75	-1.171	9	9	0	0	helix	0.733333333333333	9.18	0.0018686215322696	4.8698224852071	4.86982245520442	4.86909461472896	17.6365403604529	2	0	0	0	0	0	0	0	1	0	1	0	2	4	7	-0.136018237082067	-0.412919896640827	0.0816752744411017	-0.415686274509804	-0.481675392670157	0.547368421052632	-0.964579901153213	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	I:V:A	1.58305747959306	5.59300498642966e-05	0.392881092301541	4.02935521869819	2.23340951898872	10.6183850984524	0.687513130598596	0.0001170700255588	3.93155428682984	2.07661167855124	11.4195822840533	2.67400952353788e-05	lineage4	dr_mutations_pyrazinamide	ALA	ERR751572	ERR751572	lineage4.3.4.2	pe		pnca_p.val139ala	A	H	VAL	VA139	pnca_complex.pdb
330	V139G	L4	PZA	V	V139	139	G	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	5.113	-0.4	-2.377	4.03008	-4.222	-2.37	84	91	-1.171	9	9	0	0	helix	0.733333333333333	9.18	0.000646830530401	38.9169126950654	38.9169126367817	38.9145296299157	27.8738145664613	12	0	0	0	0	0	0	0	1	0	1	0	6	17	25	-0.101317122593718	-0.61421188630491	0.146784286016069	-0.719863597612958	-0.620418848167539	0.884210526315789	-0.964579901153213	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	I:V:A	3.66142892829214	0.0005578275078108	1.06087424525349	3.45133171502081	7.13685172053903	722.165073758294	1.59013838008863	5.5228318480997e-06	5.25783817940065	5.21074959149254	1710.75565736304	1.29490403629991e-07	lineage4	dr_mutations_pyrazinamide	GLY	SAMN01828249	SRR833062_SRR833186_SRR833060_SRR833123_SRR832980	lineage4.3.3	za	South Africa	pnca_p.val139gly	A	H	VAL	VA139	pnca_complex.pdb
331	V139L	L2	PZA	V	V139	139	L	NA	Pre-MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.113	-0.504	-0.462	-1.24107	-0.518	-0.59	79	85	-1.171	9	9	0	0	helix	0.733333333333333	9.18	0.0007905706482679	21.8981012658228	21.8980984766686	21.8894347089025	28.1227645329198	-28	0	0	0	0	0	0	0	-4	0	-1	-3	-2	0	0	-0.127659574468085	-0.11937984496124	-0.238612243328463	-0.0883205456095482	-0.154450261780105	0.831578947368421	-0.964579901153213	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	I:V:A	3.08639980550335	7.90481988040462e-05	0.781907928411257	3.9472675661117	5.63934469685405	143.700732785851	1.34040645979333	4.86279858821822e-06	5.31311371806022	4.52710002121826	207.736204499878	1.13858693465794e-07	lineage2	dr_mutations_pyrazinamide	LEU	SRS797221	SRR1723744	lineage2.2.1	cn	China:Jiangsu	pnca_p.val139leu	A	H	VAL	VA139	pnca_complex.pdb
332	V139M	L4	PZA	V	V139	139	M	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	5.113	-1.067	-0.944	-0.87501	-1.235	-1	69	80	-1.171	9	9	0	0	helix	0.733333333333333	9.18	0.000646830530401	38.9169126950654	38.9169126367816	38.9145296299157	27.8738145664613	-44	0	0	0	0	0	0	0	-6	0	-1	-5	-2	0	0	-0.270263424518744	-0.243927648578811	-0.168232331000538	-0.210571184995737	-0.261780104712042	0.726315789473684	-0.964579901153213	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.221	-1.152	9:9	147/150	I:V:A	3.66142892829214	0.0005578275078108	1.06087424525349	3.4513317150208	7.13685172054607	722.165073756559	1.59013838008863	5.5228318480997e-06	5.25783817940065	5.21074959149254	1710.75565736304	1.29490403629991e-07	lineage4	dr_mutations_pyrazinamide	MET	ERR1034990	ERR1034990	lineage4.1.1.3	za	South Africa	pnca_p.val139met	A	H	VAL	VA139	pnca_complex.pdb
333	R140G	L3	PZA	R	R140	140	G	NA	Pre-XDR	DM	hydrophilic	hydrophobic	basic	neutral	pos	non-polar	7.347	-1.735	-1.398	5.02413	-1.666	-1.21	71	85	-0.185	6	6	58	0.211678832116788	helix	-1.26666666666667	5.39	NA	NA	NA	NA	NA	70	36	0	36	4	0	2	4	1	0	0	1	6	15	17	-0.439463019250253	-0.361240310077519	0.182989750799467	-0.284057971014493	-0.316753926701571	0.747368421052632	-0.152388797364086	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	147/150	G:A:T:N:L:V:K:R:E:Y:H:F:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage3	dr_mutations_pyrazinamide	GLY	ERR3335728	ERR3335728	lineage3	pk	Pakistan	pnca_p.arg140gly	A	H	ARG	RA140	pnca_complex.pdb
334	R140H	L4	PZA	R	R140	140	H	NA	MDR	DM	hydrophilic	hydrophobic	basic	basic	pos	pos	7.347	-1.013	-1.041	2.29767	-1.276	-1.42	53	75	-0.185	6	6	58	0.211678832116788	helix	-1.26666666666667	5.39	NA	NA	NA	NA	NA	38	6	0	6	2	0	0	2	-4	0	-4	-4	-2	-2	4	-0.256585612968592	-0.268992248062015	0.0836861428186394	-0.217561807331628	-0.371727748691099	0.557894736842105	-0.152388797364086	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	147/150	G:A:T:N:L:V:K:R:E:Y:H:F:Q	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	HIS	2591	ERR2432995	lineage4.3.3	br	Brazil	pnca_p.arg140his	A	H	ARG	RA140	pnca_complex.pdb
335	R140P	L4	PZA	R	R140	140	P	NA	MDR	DM	hydrophilic	hydrophilic	basic	non-polar	pos	non-polar	7.347	-1.466	-0.818	6.54201	-1.939	-0.18	86	91	-0.185	6	6	58	0.211678832116788	helix	-1.26666666666667	5.39	0.0002156101768003	29.1287878787879	511969.848429156	Inf	9.2872640727157	66	36	0	36	6	2	2	4	-3	2	-8	-1	6	6	-2	-0.371327254305978	-0.211369509043928	0.238274244421944	-0.330605285592498	-0.0471204188481675	0.905263157894737	-0.152388797364086	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	147/150	G:A:T:N:L:V:K:R:E:Y:H:F:Q	13.1460210125008	0.90796911154963	113.719344731969	0.115600569485212	0.183926936276834	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage4	dr_mutations_pyrazinamide	PRO	SAMN01925686	SRR924238_SRR847784	lineage4.3.3	za	South Africa	pnca_p.arg140pro	A	H	ARG	RA140	pnca_complex.pdb
336	R140S	L4	PZA	R	R140	140	S	NA	MDR	DM	hydrophilic	hydrophilic	basic	neutral	pos	polar	7.347	-1.646	-1.705	4.37186	-0.933	-1.49	53	75	-0.185	6	6	58	0.211678832116788	helix	-1.26666666666667	5.39	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	64	36	0	36	2	0	0	4	1	0	-3	2	6	6	6	-0.416919959473151	-0.440568475452196	0.159232657580548	-0.159079283887468	-0.390052356020942	0.557894736842105	-0.152388797364086	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	147/150	G:A:T:N:L:V:K:R:E:Y:H:F:Q	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987745	NA	4692673.51081424	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	dr_mutations_pyrazinamide	SER	ERR2707078	ERR2707078	lineage4.3.4.2	cd	Democratic Republic of Congo	pnca_p.arg140ser	A	H	ARG	RA140	pnca_complex.pdb
337	Q141P	L2	PZA	Q	Q141	141	P	1	MDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	8.108	-1.656	0.901	5.02461	-1.835	1.14	79	85	-0.137	6	6	41	0.182222222222222	helix	-2.9	5.08	0.0019404915912031	28.1422962648557	28.1422937171073	28.1176868093208	83.7298318312042	30	-31	0	-31	4	2	0	4	3	4	-1	-2	-8	-10	-6	-0.419452887537994	0.766156462585034	0.1830072334443	-0.312872975277067	0.640449438202247	0.831578947368421	-0.112850082372323	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.332	-0.038	6:5	147/150	N:C:R:F:H:E:Y:Q:W:D:A:S	3.33727355927796	7.45865029764653e-10	0.542113710503027	6.15603976549737	10.8149160259451	96.0464157669383	1.44935953071313	1.80101596312758e-14	13.744482437837	9.60105095933572	112.1508500808	5.67227891121513e-20	lineage2	dr_mutations_pyrazinamide	PRO	ERR2517000	ERR2517000	lineage2.2.1	gb	UK	pnca_p.gln141pro	A	H	GLN	QA141	pnca_complex.pdb
338	Q141R	L4	PZA	Q	Q141	141	R	0	Sensitive	OM	hydrophilic	hydrophilic	neutral	basic	polar	pos	8.108	-1.581	0.765	1.88305	-0.131	0.13	36	66	-0.137	6	6	41	0.182222222222222	helix	-2.9	5.08	0.0001437401178668	1.2119588062211	4.59700131334267e-05	0	1.01056822389159e-25	-58	-30	0	-30	4	0	0	4	-3	0	1	-5	0	0	0	-0.400455927051672	0.650510204081633	0.068584779900786	-0.0223358908780904	0.0730337078651685	0.378947368421053	-0.112850082372323	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	-0.332	-0.038	6:5	147/150	N:C:R:F:H:E:Y:Q:W:D:A:S	-9.98752126245058	0.942832958100734	139.277183325398	-0.0717096729269461	NA	144471.706314847	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage4	other_mutations_pyrazinamide	ARG	ERR2510802	ERR2510802	lineage4.1.2.1	it	Italy	pnca_p.gln141arg	A	H	GLN	QA141	pnca_complex.pdb
339	T142A	L4	PZA	T	T142	142	A	1	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	8.098	-1.983	-0.572	2.85915	-2.289	-0.73	80	91	-0.892	8	8	0	0	helix	-0.8	8.33	0.0005749604714675	14.5802781289507	14.5802277940117	14.5801759034061	15.0663448164144	4	0	0	0	2	0	2	0	4	0	4	0	6	2	9	-0.502279635258359	-0.147803617571059	0.104136466611791	-0.390281329923274	-0.19109947643979	0.842105263157895	-0.734761120263591	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	S:E:T	2.6796663501904	0.0010245352377103	0.816050632122411	3.28370108999369	3.35697961122189	99.5538692592404	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage4	dr_mutations_pyrazinamide	ALA	364	ERR3014580	lineage4.8	tn	Tunisia:Sousse	pnca_p.thr142ala	A	H	THR	TA142	pnca_complex.pdb
340	T142K	L6	PZA	T	T142	142	K	1	MDR	DM	hydrophobic	hydrophobic	neutral	basic	polar	pos	8.098	-1.238	-0.83	3.49705	-5.128	-0.82	91	95	-0.892	8	8	0	0	helix	-0.8	8.33	0.0002156101768003	29.1287878787879	511969.848429232	Inf	9.2872640727157	-82	-48	-2	-46	2	0	2	0	1	0	3	-2	-6	-4	-6	-0.313576494427558	-0.214470284237726	0.127370173151028	-0.874339300937766	-0.214659685863874	0.957894736842105	-0.734761120263591	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	S:E:T	13.1460210125009	0.907969111549634	113.719344731975	0.115600569485207	0.183926936276726	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage6	dr_mutations_pyrazinamide	LYS	ERR2516338	ERR2516338	lineage6	be	Belgium	pnca_p.thr142lys	A	H	THR	TA142	pnca_complex.pdb
341	T142M	L2	PZA	T	T142	142	M	1	MDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	8.098	-1.836	0.175	-0.886939	-1.977	-0.23	85	91	-0.892	8	8	0	0	helix	-0.8	8.33	0.0002156101768003	9.70466975178797	9.70465898653051	9.70194990589383	2.29168245016684	-56	0	0	0	2	0	2	0	-3	0	1	-4	0	0	0	-0.465045592705167	0.148809523809524	-0.170525840190725	-0.337084398976982	-0.0602094240837696	0.894736842105263	-0.734761120263591	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	S:E:T	2.27260607808817	0.0634455902260899	1.22442760158496	1.8560559033024	0.929172866365547	208.881603004778	0.986980761010679	0.0776844925942494	1.10966566656461	0.504888890842109	570.372842410328	0.130068862702538	lineage2	dr_mutations_pyrazinamide	MET	MDR_2167	SRR6824708	lineage2.2.1	cn	China: Heping District, Tianjin	pnca_p.thr142met	A	H	THR	TA142	pnca_complex.pdb
342	T142P	L2	PZA	T	T142	142	P	NA	XDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	8.098	-1.638	-0.706	6.72536	-4.677	-0.54	92	95	-0.892	8	8	0	0	helix	-0.8	8.33	0.0001437401178668	19.411022297013	511754.463554492	Inf	4.73122070382274	4	-25	0	-25	4	2	2	0	4	4	0	-2	6	-2	4	-0.414893617021277	-0.182428940568475	0.2449522505263	-0.797442455242967	-0.141361256544503	0.968421052631579	-0.734761120263591	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	S:E:T	13.1456002256242	0.924803768447115	139.277183326822	0.0943844491367787	0.0001628371344392	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	PRO	SAMN02584649	SRR1169151_SRR1166145	lineage2.2.1	md	Moldova	pnca_p.thr142pro	A	H	THR	TA142	pnca_complex.pdb
343	T142R	L4	PZA	T	T142	142	R	NA	MDR	OM	hydrophobic	hydrophilic	neutral	basic	polar	pos	8.098	-1.267	-0.733	5.15889	-4.739	-0.89	91	95	-0.892	8	8	0	0	helix	-0.8	8.33	NA	NA	NA	NA	NA	-106	-34	0	-34	-2	0	4	-4	-9	0	0	-9	-10	-14	-4	-0.320921985815603	-0.189405684754522	0.187898003336271	-0.808013640238704	-0.232984293193717	0.957894736842105	-0.734761120263591	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	S:E:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ARG	ERR1199137	ERR1199137	lineage4.6.1.2	ug	Uganda	pnca_p.thr142arg	A	H	THR	TA142	pnca_complex.pdb
344	A143D	L3	PZA	A	A143	143	D	0	MDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	10.807	0.346	-3.46	5.46827	-5.865	-1.17	84	91	-0.834	8	8	0	0	helix	-0.8	8.82	0.0001437401178668	4.85029436501262	4.85029432717734	4.84956447904112	0.0881192621863332	-14	-46	-2	-44	0	0	0	0	-9	0	-1	-8	-6	-4	-3	0.155156950672646	-0.89405684754522	0.199166296374537	-1	-0.306282722513089	0.884210526315789	-0.686985172981878	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.955	-0.779	9:8	147/150	I:C:V:S:A:T	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673654	122.683760399096	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage3	dr_mutations_pyrazinamide	ASP	ERR2517002	ERR2517002	lineage3.1.2.1	gb	UK	pnca_p.ala143asp	A	H	ALA	AA143	pnca_complex.pdb
345	A143G	L4	PZA	A	A143	143	G	1	XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	10.807	-0.868	-2.08	2.31533	-3.708	-2.04	72	85	-0.834	8	8	0	0	helix	-0.8	8.82	0.0003593502946672	48.5888795282224	1392863.97690657	Inf	18.7541888696359	2	0	0	0	0	0	0	0	0	0	0	0	2	5	18	-0.219858156028369	-0.537467700258398	0.084329358459779	-0.632225063938619	-0.534031413612565	0.757894736842105	-0.686985172981878	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.955	-0.779	9:8	147/150	I:C:V:S:A:T	14.1468726004037	0.922400595820385	145.229797685252	0.0974102617085742	0.390278156381537	NA	1.68653688423854	0.0001456121755417	3.83680230943558	4.44801917882778	Inf	1.4869638142509e-05	lineage4	other_mutations_pyrazinamide	GLY	ERR2510826	ERR2510826	lineage4.1.2.1	it	Italy	pnca_p.ala143gly	A	H	ALA	AA143	pnca_complex.pdb
346	A143P	L1	PZA	A	A143	143	P	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	10.807	-0.732	-1.416	2.35082	-3.997	-0.21	85	91	-0.834	8	8	0	0	helix	-0.8	8.82	NA	NA	NA	NA	NA	0	0	0	0	2	2	-2	0	3	4	1	-2	-2	-2	-5	-0.185410334346505	-0.365891472868217	0.0856219815121031	-0.681500426257459	-0.0549738219895288	0.894736842105263	-0.686985172981878	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.955	-0.779	9:8	147/150	I:C:V:S:A:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	dr_mutations_pyrazinamide	PRO	SAMN07236269,SRR5709755	SRR5709755	lineage1.1.1	th	Thailand	pnca_p.ala143pro	A	H	ALA	AA143	pnca_complex.pdb
347	A143T	L2	PZA	A	A143	143	T	1	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	10.807	0.07	-2.359	0.406768	-2.811	-1.46	47	71	-0.834	8	8	0	0	helix	-0.8	8.82	0.0001437401178668	4.85029436501262	4.85029432717732	4.84956447904112	0.0881192621863332	-8	0	0	0	0	0	-2	0	-10	0	-4	-8	-6	-6	-5	0.0313901345291479	-0.609560723514212	0.0148153759861304	-0.479283887468031	-0.382198952879581	0.494736842105263	-0.686985172981878	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.955	-0.779	9:8	147/150	I:C:V:S:A:T	1.57903938912401	0.264247566376724	1.41439286276584	1.11640791656443	0.191756076673653	122.683760399095	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	dr_mutations_pyrazinamide	THR	0802Y-sc-2012-02-09T18:57:12Z-1355278,ERR133945	ERR133945	lineage2.2.1	ru	Russia	pnca_p.ala143thr	A	H	ALA	AA143	pnca_complex.pdb
348	A143V	L2	PZA	A	A143	143	V	0	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.807	-1.168	-0.622	0.113853	-1.998	-0.56	-16	57	-0.834	8	8	0	0	helix	-0.8	8.82	0.0003593502946672	0.484657419083649	1.69068633684891e-05	0	0.177777263899814	-8	0	0	0	0	0	0	0	-12	0	-6	-6	-2	-2	-7	-0.295845997973658	-0.160723514211886	0.00414677408780658	-0.340664961636829	-0.146596858638743	-0.172043010752688	-0.686985172981878	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.955	-0.779	9:8	147/150	I:C:V:S:A:T	-10.9877909019996	0.939691219428539	145.229797681912	-0.0756579646696575	NA	60.3389082676124	-0.314565134742567	0.596111875058711	0.224672226566594	0	5.29224350433385	0.673290424524484	lineage2	other_mutations_pyrazinamide	VAL	SRR2100911,SAMN03648084	SRR2100911	lineage2.2.1			pnca_p.ala143val	A	H	ALA	AA143	pnca_complex.pdb
349	D145E	L2	PZA	D	D145	145	E	NA	MDR	DM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	11.649	-1.031	-0.148	-0.4769	-0.323	-0.78	70	85	-1.092	9	9	33	0.170984455958549	helix	-1.73333333333333	4.71	7.18700589334483e-05	9.70142977291842	188179.926264557	Inf	0.763784339928386	-10	4	0	4	2	0	-2	4	-3	0	-2	-1	0	2	-2	-0.261144883485309	-0.0382428940568475	-0.0916903791432746	-0.0550724637681159	-0.204188481675393	0.736842105263158	-0.899505766062603	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.152	-1.062	9:9	147/150	S:E:A:D:K	12.145153838726	0.919026337424219	119.468044436206	0.101660271548274	5.01153666065157e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	dr_mutations_pyrazinamide	GLU	SAMN02360576	SRR1013594_SRR1048979_SRR1013593_SRR1048978	lineage2.2.2	kr	South Korea	pnca_p.asp145glu	A	H	ASP	DA145	pnca_complex.pdb
350	D145Y	L4	PZA	D	D145	145	Y	1	Pre-MDR	OM	hydrophilic	hydrophobic	acidic	neutral	neg	polar	11.649	-1.689	-0.409	-0.814413	-0.858	-1.05	82	91	-1.092	9	9	33	0.170984455958549	helix	-1.73333333333333	4.71	7.18700589334483e-05	9.70142977291842	188179.926264644	Inf	0.763784339928386	-38	28	0	28	0	0	-4	6	-1	0	0	-2	-2	-2	-2	-0.427811550151976	-0.105684754521964	-0.1565817503653	-0.146291560102302	-0.274869109947644	0.863157894736842	-0.899505766062603	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.152	-1.062	9:9	147/150	S:E:A:D:K	12.1451538387265	0.919026337424236	119.468044436235	0.101660271548254	5.01153666062444e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	TYR	ERR2515552	ERR2515552	lineage4.1.1.3	za	South Africa	pnca_p.asp145tyr	A	H	ASP	DA145	pnca_complex.pdb
351	A146E	L2	PZA	A	A146	146	E	NA	XDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	14.858	0.284	-2.184	3.30206	-5.327	-0.93	75	85	-0.895	8	8	0	0	helix	0.833333333333333	7.08	0.0001437401178668	19.411022297013	511754.463553649	Inf	4.73122070382274	-36	-46	-2	-44	0	0	-2	0	-6	0	-2	-7	-10	-8	-9	0.127354260089686	-0.564341085271318	0.120268212909476	-0.908269394714407	-0.243455497382199	0.789473684210526	-0.737232289950577	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	G:S:A	13.1456002256226	0.924803768447062	139.277183326707	0.0943844491368447	0.0001628371344419	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	GLU	SAMN02628508	SRR1174308_SRR1173552	lineage2.2.1	ir	Iran	pnca_p.ala146glu	A	H	ALA	AA146	pnca_complex.pdb
352	A146P	L4	PZA	A	A146	146	P	NA	Pre-MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	14.858	-1.497	-1.233	3.10964	-3.961	0.02	79	85	-0.895	8	8	0	0	helix	0.833333333333333	7.08	7.18700589334483e-05	9.70142977291842	188179.926264681	Inf	0.763784339928386	0	0	0	0	2	2	-2	0	-1	4	-5	-4	-4	-2	-5	-0.379179331306991	-0.318604651162791	0.113259857662133	-0.67536231884058	0.0112359550561797	0.831578947368421	-0.737232289950577	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	-0.993	-0.827	9:8	147/150	G:S:A	12.1451538387267	0.919026337424242	119.468044436246	0.101660271548246	5.01153666061373e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	PRO	NG70	ERR1679638	lineage4.6	ng	Nigeria	pnca_p.ala146pro	A	H	ALA	AA146	pnca_complex.pdb
353	A146T	L4	PZA	A	A146	146	T	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	14.858	-0.252	-2.074	1.48094	-2.71	-1.44	53	75	-0.895	8	8	0	0	helix	0.833333333333333	7.08	0.0007187005893344	7.28887484197219	7.28887438834092	7.28728068125885	10.1421736186998	0	0	0	0	-2	0	-4	0	-5	0	-1	-4	-6	-2	-7	-0.0638297872340425	-0.535917312661499	0.053939058413887	-0.462063086104007	-0.37696335078534	0.557894736842105	-0.737232289950577	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	G:S:A	1.9863491292074	0.0021008470164788	0.645842738542518	3.07559257179235	2.08051219809968	28.5295272509804	0.862660492976905	0.0027830995390782	2.55547126060833	1.72659076531017	35.1741774538487	0.0014491518456837	lineage4	dr_mutations_pyrazinamide	THR	SRR3724799	SRR3724799	lineage4.3.4.2.1	cd	DRC	pnca_p.ala146thr	A	H	ALA	AA146	pnca_complex.pdb
354	A146V	L4	PZA	A	A146	146	V	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	14.858	-1.712	-0.425	0.463909	-2.215	-0.75	63	80	-0.895	8	8	0	0	helix	0.833333333333333	7.08	0.0010061808250682	63.3736263736264	63.3734135554618	63.3515540724066	51.5211006732107	0	0	0	0	0	0	0	0	-9	0	-3	-8	-4	-2	-5	-0.433637284701115	-0.109819121447028	0.0168965755869434	-0.37766410912191	-0.196335078534031	0.663157894736842	-0.737232289950577	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	147/150	G:S:A	4.14904442894259	6.28168511875833e-05	1.03675010392783	4.0019715582603	12.6188969963258	1151.617862186	1.8019085590898	1.22416292455872e-09	8.91216077783748	9.50473092944164	2653.71554384332	7.08317026236427e-13	lineage4	dr_mutations_pyrazinamide	VAL	SAMN02567715	SRR1166171	lineage4.8	ro	Romania	pnca_p.ala146val	A	H	ALA	AA146	pnca_complex.pdb
355	R148C	L2	PZA	R	R148	148	C	NA	Pre-XDR	OM	hydrophilic	hydrophobic	basic	neutral	pos	non-polar	14.673	-0.94	-0.277	1.80299	-0.806	0.84	39	66	1.306	1	1	156	0.569343065693431	helix	-1.26666666666667	3.5	NA	NA	NA	NA	NA	38	22	0	22	0	0	0	2	-3	0	-7	3	6	6	4	-0.238095238095238	-0.0715762273901808	0.0656688204313843	-0.137425404944587	0.47191011235955	0.410526315789474	0.418589743589744	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.842	1.712	2:1	146/150	I:R:K:N:L:Q:E:H:W:D:M:S:A:G:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	CYS	SAMN02414984	SRR1166878_SRR1162765_SRR1165490_SRR1165550	lineage2.2.1	se	Sweden	pnca_p.arg148cys	A	H	ARG	RA148	pnca_complex.pdb
356	N149D	L4	PZA	N	N149	149	D	NA	Sensitive	OM	hydrophilic	hydrophilic	neutral	acidic	polar	neg	16.469	-0.043	0.469	1.84951	-0.656	0.16	-34	66	0.97	1	1	71	0.364102564102564	loop	-2.8	3.68	NA	NA	NA	NA	NA	0	-5	0	-5	0	0	2	0	0	0	0	0	0	0	2	-0.0108915906788247	0.398809523809524	0.0673631800930951	-0.111849957374254	0.0898876404494382	-0.365591397849462	0.310897435897436	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	neutral	0.59	1.183	3:1	146/150	Q:E:Y:H:F:C:R:K:L:N:G:M:S:A:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ASP	SRR5065677	SRR5065677	lineage4.1.2.1	vn	Vietnam	pnca_p.asn149asp	A	T	ASN	NA149	pnca_complex.pdb
357	N149S	L2	PZA	N	N149	149	S	NA	Pre-MDR	OM	hydrophilic	hydrophilic	neutral	neutral	polar	polar	16.469	-0.233	-0.42	0.889156	-0.206	0.47	-76	87	0.97	1	1	71	0.364102564102564	loop	-2.8	3.68	NA	NA	NA	NA	NA	18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	-0.0590172239108409	-0.108527131782946	0.0323849969769593	-0.0351236146632566	0.264044943820225	-0.817204301075269	0.310897435897436	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	neutral	0.59	1.183	3:1	146/150	Q:E:Y:H:F:C:R:K:L:N:G:M:S:A:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	SER	SRR5074122	SRR5074122	lineage2.2.2	vn	Vietnam	pnca_p.asn149ser	A	T	ASN	NA149	pnca_complex.pdb
358	N149V	L2	PZA	N	N149	149	V	1	Pre-XDR	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	16.469	-0.706	-0.064	0.446809	-0.317	-0.26	-28	61	0.97	1	1	71	0.364102564102564	loop	-2.8	3.68	7.18700589334483e-05	9.70142977291842	188179.926264649	Inf	0.763784339928386	16	0	0	0	0	0	2	0	-3	0	-3	0	0	0	-2	-0.178824721377913	-0.0165374677002583	0.0162737563647754	-0.0540494458653026	-0.0680628272251308	-0.301075268817204	0.310897435897436	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.59	1.183	3:1	146/150	Q:E:Y:H:F:C:R:K:L:N:G:M:S:A:D	12.1451538387265	0.919026337424236	119.468044436237	0.101660271548253	5.01153666062283e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	VAL	MDR_12332	SRR6824501	lineage2.2.1	cn	China: Xuanzhou District, Xuancheng City, Anhui Province	pnca_p.asn149val	A	T	ASN	NA149	pnca_complex.pdb
359	G150D	L4	PZA	G	G150	150	D	NA	MDR	OM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	21.16	1.269	-0.785	3.55888	-0.949	-0.34	51	75	-0.715	8	8	63	0.605769230769231	loop	-0.0333333333333334	3.34	7.18700589334483e-05	9.70142977291842	188179.926264446	Inf	0.763784339928386	-12	-18	-2	-16	0	0	0	0	-1	0	-3	0	-2	-3	-9	0.569058295964126	-0.202842377260982	0.129622156338551	-0.161807331628303	-0.0890052356020942	0.536842105263158	-0.588962108731466	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.615	8:7	146/150	G:E:H:Q:L:D:R	12.1451538387254	0.9190263374242	119.468044436172	0.101660271548298	5.01153666068333e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	ASP	ERR2179651	ERR2179651	lineage4.4.2	cn	China	pnca_p.gly150asp	A	T	GLY	GA150	pnca_complex.pdb
360	L151S	L4	PZA	L	L151	151	S	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	18.507	0.079	-2.523	3.86505	-2.175	-2.07	57	75	-0.04	5	5	10	0.0497512437810945	loop	1.73333333333333	5.31	0.0010061808250682	8.75696202531646	8.7569577260426	8.75484033225151	18.8086142562631	18	0	0	0	0	0	0	-2	-3	0	-3	0	10	4	8	0.0354260089686098	-0.651937984496124	0.140773534189497	-0.370843989769821	-0.541884816753927	0.6	-0.0329489291598023	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	145/150	Q:F:Y:I:R:L:V:M:W	2.16984855308098	0.0001010560982854	0.558082575591327	3.888042106998	3.02252426488638	28.523060095548	0.942353466387901	0.0001077244819236	3.96768558587492	2.63211458386555	33.2911283333248	1.44512946670157e-05	lineage4	dr_mutations_pyrazinamide	SER	SAMN02403582	SRR1169250_SRR1167347_SRR1165654_SRR1169315	lineage4.8	za	South Africa	pnca_p.leu151ser	A	-	LEU	LA151	pnca_complex.pdb
361	L151V	L2	PZA	L	L151	151	V	NA	Other	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	18.507	-1.269	-1.74	1.09305	-1.108	-1.93	31	66	-0.04	5	5	10	0.0497512437810945	loop	1.73333333333333	5.31	NA	NA	NA	NA	NA	18	0	0	0	0	0	0	0	-5	0	-1	-4	-2	-2	2	-0.321428571428571	-0.449612403100775	0.0398112602801594	-0.188917306052856	-0.505235602094241	0.326315789473684	-0.0329489291598023	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	145/150	Q:F:Y:I:R:L:V:M:W	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	VAL	SRR5067646	SRR5067646	lineage2.2.1.1	vn	Vietnam	pnca_p.leu151val	A	-	LEU	LA151	pnca_complex.pdb
362	L151W	L2;L4	PZA	L	L151	151	W	1	XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	18.507	-0.714	-1.831	2.01402	-0.33	-2.26	20	63	-0.04	5	5	10	0.0497512437810945	loop	1.73333333333333	5.31	7.18700589334483e-05	9.70142977291842	188179.926264453	Inf	0.763784339928386	-22	0	0	0	0	0	0	0	-11	0	-3	-10	-10	-16	-2	-0.180851063829787	-0.47312661498708	0.0733549923877651	-0.0562659846547315	-0.591623036649215	0.210526315789474	-0.0329489291598023	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	145/150	Q:F:Y:I:R:L:V:M:W	12.1451538387255	0.919026337424201	119.468044436174	0.101660271548297	5.01153666068148e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2;lineage4	other_mutations_pyrazinamide	TRP	XTB13-245	SRR1172073_SRR1163374_SRR1163172_SRR1169046	lineage4.3.3;lineage2.2.1	by	Belarus	pnca_p.leu151trp	A	-	LEU	LA151	pnca_complex.pdb
363	T153I	L4	PZA	T	T153	153	I	NA	Pre-MDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	15.544	-2.111	0.532	-0.698929	-1.456	-0.46	43	71	-0.188	6	6	12	0.0697674418604651	strand	-1.13333333333333	5.38	NA	NA	NA	NA	NA	-20	0	0	0	2	0	2	0	-11	0	-5	-8	-2	-4	-2	-0.534701114488348	0.452380952380952	-0.134378412673998	-0.248252344416027	-0.120418848167539	0.452631578947368	-0.154859967051071	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	144/150	V:C:A:S:T	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ILE	SRR5067274	SRR5067274	lineage4.2.2	vn	Vietnam	pnca_p.thr153ile	A	E	THR	TA153	pnca_complex.pdb
364	T153P	L2	PZA	T	T153	153	P	1	Pre-XDR	OM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	15.544	-2.212	-0.926	3.60938	-4.405	-0.6	70	85	-0.188	6	6	12	0.0697674418604651	strand	-1.13333333333333	5.38	7.18700589334483e-05	9.70142977291842	188179.926264634	Inf	0.763784339928386	0	0	0	0	2	0	2	0	-6	0	-6	-2	6	8	0	-0.560283687943262	-0.239276485788114	0.131461476263667	-0.751065643648764	-0.157068062827225	0.736842105263158	-0.154859967051071	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.038	7:5	144/150	V:C:A:S:T	12.1451538387264	0.919026337424234	119.468044436232	0.101660271548256	5.0115366606273e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	PRO	SAMN10961392	SRR8651662	lineage2.2.1	cn	China:Wenzhou	pnca_p.thr153pro	A	E	THR	TA153	pnca_complex.pdb
365	R154G	L4	PZA	R	R154	154	G	NA	XDR	DM	hydrophilic	hydrophobic	basic	neutral	pos	non-polar	15.259	-2.707	-1.337	4.31639	-1.992	-1.71	73	85	1.542	1	1	60	0.218978102189781	strand	-0.333333333333333	5.21	0.0011499209429351	34.1357293868922	34.1353729715529	34.1417078158954	51.1500808664409	66	28	4	24	2	0	0	2	0	0	-1	1	10	11	21	-0.685663627152989	-0.345478036175711	0.157212319437059	-0.339641943734015	-0.447643979057592	0.768421052631579	0.494230769230769	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.842	1.712	2:1	144/150	T:W:E:F:Q:V:N:L:R:I:C:S:A:D:Y:H	3.530334177178	2.90603701922833e-06	0.754772029037902	4.6773516258652	9.53076902254824	217.507266299408	1.53320918696851	1.50118436279358e-09	8.82356596809844	7.82937374234326	309.275011521707	8.55671558873616e-13	lineage4	dr_mutations_pyrazinamide	GLY	SAMN02403532	SRR1165336_SRR1167260_SRR1173456_SRR1173876	lineage4.1.1.3	za	South Africa	pnca_p.arg154gly	A	E	ARG	RA154	pnca_complex.pdb
366	R154S	L2	PZA	R	R154	154	S	NA	MDR	OM	hydrophilic	hydrophilic	basic	neutral	pos	polar	15.259	-2.614	-2.044	2.37151	-1.076	-1.74	55	75	1.542	1	1	60	0.218978102189781	strand	-0.333333333333333	5.21	NA	NA	NA	NA	NA	60	28	4	24	0	0	0	0	3	0	0	3	10	4	6	-0.662107396149949	-0.528165374677003	0.0863755563487496	-0.183461210571185	-0.455497382198953	0.578947368421053	0.494230769230769	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.842	1.712	2:1	144/150	T:W:E:F:Q:V:N:L:R:I:C:S:A:D:Y:H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	SER	ERR1023404	ERR1023404	lineage2.2.1			pnca_p.arg154ser	A	E	ARG	RA154	pnca_complex.pdb
367	R154W	L2	PZA	R	R154	154	W	NA	MDR	OM	hydrophilic	hydrophobic	basic	non-polar	pos	non-polar	15.259	-1.381	-0.436	2.7463	-0.368	-0.6	56	75	1.542	1	1	60	0.218978102189781	strand	-0.333333333333333	5.21	NA	NA	NA	NA	NA	10	28	4	24	2	0	0	2	-4	0	-3	-1	10	4	6	-0.349797365754813	-0.11266149870801	0.100026223967249	-0.0627450980392157	-0.157068062827225	0.589473684210526	0.494230769230769	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.842	1.712	2:1	144/150	T:W:E:F:Q:V:N:L:R:I:C:S:A:D:Y:H	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	TRP	SRS796973	SRR1723461	lineage2.2.1	cn	China:Shaanxi	pnca_p.arg154trp	A	E	ARG	RA154	pnca_complex.pdb
368	V155A	L2;L4	PZA	V	V155	155	A	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	12.302	-1.886	-3.049	3.6527	-2.972	-2.61	54	75	-0.482	7	7	0	0	strand	1.16666666666667	7.97	7.18700589334483e-05	9.70142977291842	188179.926264589	Inf	0.763784339928386	2	0	0	0	0	0	0	0	0	0	0	0	0	2	3	-0.477710233029382	-0.787855297157623	0.133039284959826	-0.506734867860188	-0.683246073298429	0.568421052631579	-0.397034596375618	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.615	-0.411	7:7	144/150	F:Y:I:L:V	12.1451538387262	0.919026337424226	119.468044436217	0.101660271548266	5.0115366606409e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2;lineage4	dr_mutations_pyrazinamide	ALA	ERR1213939	ERR1213939	lineage4.3;lineage2.2.1	pe	Peru	pnca_p.val155ala	A	E	VAL	VA155	pnca_complex.pdb
369	V155G	L2	PZA	V	V155	155	G	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	12.302	-1.655	-3.675	5.87683	-4.368	-3.48	72	85	-0.482	7	7	0	0	strand	1.16666666666667	7.97	0.0004312203536006	24.2923336141533	24.2923334241601	24.2833096922897	14.1977442691074	4	0	0	0	0	0	0	0	0	0	0	0	6	3	25	-0.41919959473151	-0.949612403100775	0.214046940901376	-0.744757033248082	-0.910994764397906	0.757894736842105	-0.397034596375618	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.615	-0.411	7:7	144/150	F:Y:I:L:V	3.19016080361873	0.0035954281300594	1.09565968954934	2.91163472932996	3.91551977432402	465.531063220228	1.38546923679232	0.0007494070007895	3.12528225414311	2.71567844828345	1141.60089496884	0.0001645676755807	lineage2	dr_mutations_pyrazinamide	GLY	SAMN03653311,SRS935922	SRR2024894	lineage2.2.1	cn	China	pnca_p.val155gly	A	E	VAL	VA155	pnca_complex.pdb
370	V155L	L4	PZA	V	V155	155	L	1	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	12.302	-2.349	-1.141	-0.756754	-1.463	-1.05	-14	57	-0.482	7	7	0	0	strand	1.16666666666667	7.97	7.18700589334483e-05	9.70142977291842	188179.926264586	Inf	0.763784339928386	-34	0	0	0	0	0	0	0	-6	0	-2	-4	-8	-8	-2	-0.594984802431611	-0.294832041343669	-0.145496039375529	-0.249445865302643	-0.274869109947644	-0.150537634408602	-0.397034596375618	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	-0.615	-0.411	7:7	144/150	F:Y:I:L:V	12.1451538387262	0.919026337424224	119.468044436215	0.101660271548268	5.01153666064309e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	LEU	ERR2864270	ERR2864270	lineage4.1	pt	Portugal	pnca_p.val155leu	A	E	VAL	VA155	pnca_complex.pdb
371	V155M	L2	PZA	V	V155	155	M	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	12.302	-2.486	-1.505	-0.575554	-2.196	-1.23	51	75	-0.482	7	7	0	0	strand	1.16666666666667	7.97	0.0004312203536006	58.3312262958281	1393450.94022009	Inf	23.547488635408	-48	0	0	0	0	0	0	0	-8	0	0	-8	-4	-4	0	-0.629685916919959	-0.388888888888889	-0.110657925094209	-0.374424552429668	-0.321989528795811	0.536842105263158	-0.397034596375618	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.615	-0.411	7:7	144/150	F:Y:I:L:V	14.1472939191273	0.915018435774946	132.576060676167	0.106710773023222	4.1789618205157	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage2	dr_mutations_pyrazinamide	MET	SRS797250	SRR1723776	lineage2.2.1	cn	China:Heilongjiang	pnca_p.val155met	A	E	VAL	VA155	pnca_complex.pdb
372	L156P	L2	PZA	L	L156	156	P	1	XDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	13.994	-1.81	-2.813	2.74955	-3.96	-1.74	63	80	0.122	5	5	0	0	strand	4.06666666666667	6.53	0.0001437401178668	19.411022297013	511754.463553313	Inf	4.73122070382274	18	0	0	0	2	2	0	0	4	2	0	2	0	-2	-2	-0.458459979736575	-0.72687338501292	0.100144596041638	-0.675191815856777	-0.455497382198953	0.663157894736842	0.0391025641025641	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	144/150	L:V:N:R:I:K:F:H:P	13.1456002256219	0.924803768447042	139.277183326662	0.0943844491368703	0.000162837134443	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	dr_mutations_pyrazinamide	PRO	ERR108455,0716E-sc-2011-10-12T14:58:55Z-1289120	ERR108455	lineage2.2.1	ru	Russia,Samara	pnca_p.leu156pro	A	E	LEU	LA156	pnca_complex.pdb
373	L156Q	L4	PZA	L	L156	156	Q	1	Pre-MDR	OM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	13.994	-0.36	-2.863	2.41139	-4.191	-2.69	41	71	0.122	5	5	0	0	strand	4.06666666666667	6.53	7.18700589334483e-05	9.70142977291842	188179.92626469	Inf	0.763784339928386	-14	0	0	0	-2	0	0	-2	3	0	-2	5	0	0	0	-0.0911854103343465	-0.739793281653747	0.0878280727569403	-0.71457800511509	-0.704188481675393	0.431578947368421	0.0391025641025641	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.147	0.225	6:4	144/150	L:V:N:R:I:K:F:H:P	12.1451538387267	0.919026337424244	119.46804443625	0.101660271548243	5.01153666061033e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	other_mutations_pyrazinamide	GLN	ERR038749	ERR038749	lineage4.6.1.2	ug	Uganda	pnca_p.leu156gln	A	E	LEU	LA156	pnca_complex.pdb
374	V157A	L2	PZA	V	V157	157	A	1	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.632	-0.931	-1.625	0.858959	-1.03	-1.74	-36	66	0.579	3	3	45	0.258620689655172	strand	1.5	4.12	7.18700589334483e-05	9.70142977291842	188179.926264427	Inf	0.763784339928386	0	0	0	0	0	0	0	0	3	0	3	0	2	0	5	-0.235815602836879	-0.419896640826873	0.0312851565060934	-0.175618073316283	-0.455497382198953	-0.387096774193548	0.185576923076923	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.225	0.842	4:2	144/150	Q:F:E:I:R:L:V:T:W:K:P:S:M:G:A:D	12.1451538387253	0.919026337424197	119.468044436166	0.101660271548303	5.01153666068889e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ALA	ERR2516727	ERR2516727	lineage2.2.1.1	pe	Peru	pnca_p.val157ala	A	E	VAL	VA157	pnca_complex.pdb
375	V157G	L2	PZA	V	V157	157	G	1	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	13.632	-0.926	-1.896	1.97999	-1.919	-2.35	-7	53	0.579	3	3	45	0.258620689655172	strand	1.5	4.12	7.18700589334483e-05	9.70142977291842	188179.926264401	Inf	0.763784339928386	-2	0	0	0	0	0	0	0	3	0	3	0	2	3	17	-0.234549138804458	-0.489922480620155	0.072115545713474	-0.327195225916454	-0.615183246073298	-0.0752688172043011	0.185576923076923	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.225	0.842	4:2	144/150	Q:F:E:I:R:L:V:T:W:K:P:S:M:G:A:D	12.1451538387252	0.919026337424192	119.468044436158	0.101660271548309	5.01153666069661e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	GLY	MDR_12366	SRR6824636	lineage2.2.1	cn	China: Xuanzhou District, Xuancheng City, Anhui Province	pnca_p.val157gly	A	E	VAL	VA157	pnca_complex.pdb
376	V157L	L4	PZA	V	V157	157	L	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	13.632	-0.972	-0.484	-2.04339	1.184	-0.57	-43	72	0.579	3	3	45	0.258620689655172	strand	1.5	4.12	NA	NA	NA	NA	NA	-18	0	0	0	2	0	-2	2	-3	0	1	-4	-4	-2	-2	-0.246200607902736	-0.125064599483204	-0.392868953318465	1	-0.149214659685864	-0.462365591397849	0.185576923076923	Destabilising	Destabilising	Stabilising	Stabilising	Destabilising	neutral	0.225	0.842	4:2	144/150	Q:F:E:I:R:L:V:T:W:K:P:S:M:G:A:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	LEU	ERR1198987	ERR1198987	lineage4.6.1.1	ug	Uganda	pnca_p.val157leu	A	E	VAL	VA157	pnca_complex.pdb
377	L159P	L4	PZA	L	L159	159	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	11.435	-1.752	-1.412	6.08412	-4.496	-0.94	92	95	-0.599	7	7	6	0.0298507462686567	strand	-0.133333333333333	5.42	NA	NA	NA	NA	NA	24	4	0	4	2	2	2	0	2	4	-4	0	-4	-2	-2	-0.443768996960486	-0.364857881136951	0.221596893916768	-0.766581415174766	-0.246073298429319	0.968421052631579	-0.49341021416804	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	141/150	G:A:M:H:F:Y:L:V	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	PRO	ERR1034843	ERR1034843	lineage4.7	pt	Portugal	pnca_p.leu159pro	A	E	LEU	LA159	pnca_complex.pdb
378	L159R	L2	PZA	L	L159	159	R	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	11.435	-1.154	-0.753	0.0571381999999999	-4.133	-0.41	89	91	-0.599	7	7	6	0.0298507462686567	strand	-0.133333333333333	5.42	0.0005030904125341	68.0817875210792	1394038.39846606	Inf	28.35828361563	-56	-26	-2	-24	-4	0	-2	-2	-3	0	0	-5	-4	-4	0	-0.292299898682877	-0.194573643410853	0.00208109761871808	-0.704688832054561	-0.107329842931937	0.936842105263158	-0.49341021416804	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	141/150	G:A:M:H:F:Y:L:V	14.1477154154513	0.908235680147999	122.741582019401	0.115264242017143	22.9578143501753	NA	1.83303094984313	4.24048522418865e-06	5.37258444573496	7.00237574052121	Inf	1.00814491180914e-07	lineage2	dr_mutations_pyrazinamide	ARG	SRR5065596	SRR5065596	lineage2.2.1	vn	Vietnam	pnca_p.leu159arg	A	E	LEU	LA159	pnca_complex.pdb
379	L159V	L2	PZA	L	L159	159	V	0	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.435	-1.82	-1.126	2.84083	-2.332	-1.09	60	80	-0.599	7	7	6	0.0298507462686567	strand	-0.133333333333333	5.42	0.0001437401178668	4.85029436501262	4.85029432717735	4.84956447904112	0.0881192621863332	22	4	0	4	0	0	0	0	-8	0	-2	-6	10	-2	8	-0.460992907801418	-0.290956072351421	0.103469212334006	-0.397612958226769	-0.285340314136126	0.631578947368421	-0.49341021416804	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.728	-0.485	8:7	141/150	G:A:M:H:F:Y:L:V	1.57903938912402	0.264247566376723	1.41439286276584	1.11640791656443	0.191756076673846	122.683760399179	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	dr_mutations_pyrazinamide	VAL	ERS458597,SAMEA2535195	ERR553180_ERR553181	lineage2.2.1	uz	Nukus	pnca_p.leu159val	A	E	LEU	LA159	pnca_complex.pdb
380	T160A	L2	PZA	T	T160	160	A	1	XDR	DM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	9.847	-2.509	-0.842	2.1045	-1.519	-0.73	57	75	-0.228	6	6	22	0.127906976744186	strand	1.63333333333333	4.98	0.0005749604714675	77.8405735976381	1394626.35224664	Inf	33.1802749271012	8	0	0	0	2	0	2	0	0	0	0	0	2	4	3	-0.635511651469098	-0.217571059431524	0.0766504709387452	-0.258994032395567	-0.19109947643979	0.6	-0.187808896210873	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.147	7:6	141/150	V:C:I:M:S:T	14.1481370895081	0.901927875940744	114.814237034519	0.123226330243822	82.9586021395589	NA	1.89120602753485	7.2326389241213e-07	6.1407032156959	8.29603358299821	Inf	8.39983046099388e-09	lineage2	dr_mutations_pyrazinamide	ALA	ERR1873556	ERR1873556	lineage2.2.1	za	South Africa	pnca_p.thr160ala	A	E	THR	TA160	pnca_complex.pdb
381	T160P	L2	PZA	T	T160	160	P	NA	XDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	9.847	-2.375	-0.79	3.37194	-3.753	-0.23	91	95	-0.228	6	6	22	0.127906976744186	strand	1.63333333333333	4.98	0.0002156101768003	29.1287878787879	511969.848428359	Inf	9.2872640727157	6	0	0	0	2	0	2	0	-4	2	-6	0	0	0	-4	-0.601570415400203	-0.204134366925065	0.122813394619716	-0.639897698209719	-0.0602094240837696	0.957894736842105	-0.187808896210873	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.147	7:6	141/150	V:C:I:M:S:T	13.1460210124992	0.907969111549571	113.719344731883	0.115600569485286	0.183926936278634	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage2	dr_mutations_pyrazinamide	PRO	SRR5710026,SAMN07236538	SRR5710026	lineage2.2.1	th	Thailand	pnca_p.thr160pro	A	E	THR	TA160	pnca_complex.pdb
382	T160R	L4	PZA	T	T160	160	R	NA	MDR	DM	hydrophobic	hydrophilic	neutral	basic	polar	pos	9.847	-2.007	-0.456	2.8141	-2.869	-0.29	83	91	-0.228	6	6	22	0.127906976744186	strand	1.63333333333333	4.98	0.0001437401178668	19.411022297013	511754.463552351	Inf	4.73122070382274	-80	-36	-2	-34	0	0	-2	0	-5	0	-2	-3	-8	-2	-4	-0.508358662613982	-0.117829457364341	0.10249564754988	-0.489173060528559	-0.0759162303664921	0.873684210526316	-0.187808896210873	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.411	-0.147	7:6	141/150	V:C:I:M:S:T	13.14560022562	0.924803768446983	139.277183326532	0.0943844491369454	0.000162837134446	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	ARG	SRR4034747	SRR4034747	lineage4.4.1.1	za	South Africa: Kwazulu-Natal	pnca_p.thr160arg	A	E	THR	TA160	pnca_complex.pdb
383	A161G	L2	PZA	A	A161	161	G	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	7.033	-0.925	-0.745	2.35053	-1.432	-1.02	50	75	-0.773	8	8	11	0.0852713178294573	strand	0.233333333333333	4.53	NA	NA	NA	NA	NA	10	0	0	0	0	0	0	0	0	0	0	0	2	11	10	-0.234295845997974	-0.19250645994832	0.0856114190808499	-0.244160272804774	-0.267015706806283	0.526315789473684	-0.63673805601318	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.872	-0.728	8:8	141/150	E:Q:V:I:R:K:S:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	GLY	SRS797195	SRR1723714	lineage2.2.1	cn	China:Shanxi	pnca_p.ala161gly	A	E	ALA	AA161	pnca_complex.pdb
384	A161V	L1	PZA	A	A161	161	V	NA	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	7.033	-0.846	0.043	-2.66025	0.125	-0.2	21	63	-0.773	8	8	11	0.0852713178294573	strand	0.233333333333333	4.53	NA	NA	NA	NA	NA	-2	0	0	0	0	0	0	0	-5	0	-1	-4	-2	-4	-3	-0.214285714285714	0.0365646258503401	-0.511468507267554	0.105574324324324	-0.0523560209424083	0.221052631578947	-0.63673805601318	Destabilising	Stabilising	Stabilising	Stabilising	Destabilising	effect	-0.872	-0.728	8:8	141/150	E:Q:V:I:R:K:S:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	VAL	ERR752069	ERR752069	lineage1.1.1	th	Thailand	pnca_p.ala161val	A	E	ALA	AA161	pnca_complex.pdb
385	G162A	L4	PZA	G	G162	162	A	NA	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	8.315	-0.164	-0.551	0.560964	-1.074	-0.09	10	59	-0.02	5	5	26	0.25	loop	1.86666666666667	3.93	NA	NA	NA	NA	NA	-6	0	0	0	0	0	0	0	-4	0	-2	-2	0	-1	-12	-0.0415400202634245	-0.142377260981912	0.0204315299499559	-0.183120204603581	-0.0235602094240837	0.105263157894737	-0.0164744645799012	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	141/150	F:S:G:A:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	ALA	SAMN02584635	SRR1166166_SRR1169042	lineage4.2.1	md	Moldova	pnca_p.gly162ala	A	-	GLY	GA162	pnca_complex.pdb
386	G162D	L4	PZA	G	G162	162	D	1	MDR	DM	hydrophobic	hydrophilic	neutral	acidic	non-polar	neg	8.315	2.23	-1.037	3.43178	-1.558	-0.94	79	85	-0.02	5	5	26	0.25	loop	1.86666666666667	3.93	0.0002874802357337	38.8547368421053	1392277.5078503	Inf	13.9909822352959	-20	-24	0	-24	0	0	0	0	-6	0	0	-4	-4	-5	-19	1	-0.267958656330749	0.124992897675537	-0.265643648763853	-0.246073298429319	0.831578947368421	-0.0164744645799012	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	141/150	F:S:G:A:P	14.146451459095	0.930573112990214	162.371849599053	0.0871237932808364	0.0113995443355929	NA	1.58944397188817	0.0008528275207519	3.06913879340442	3.20302487654751	Inf	0.0001836895199759	lineage4	dr_mutations_pyrazinamide	ASP	ERR038742	ERR038742	lineage4.8	ug	Uganda	pnca_p.gly162asp	A	-	GLY	GA162	pnca_complex.pdb
387	G162R	L5	PZA	G	G162	162	R	NA	Sensitive	OM	hydrophobic	hydrophilic	neutral	basic	non-polar	pos	8.315	-0.04	-0.44	3.42463	-0.961	-0.15	87	91	-0.02	5	5	26	0.25	loop	1.86666666666667	3.93	NA	NA	NA	NA	NA	-68	-22	0	-22	0	0	0	0	-7	0	-3	-5	-2	-5	-17	-0.0101317122593718	-0.113695090439276	0.124732479111882	-0.16385336743393	-0.0392670157068062	0.91578947368421	-0.0164744645799012	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	141/150	F:S:G:A:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage5	other_mutations_pyrazinamide	ARG	ERR702413	ERR702413	lineage5	gh	Ghana	pnca_p.gly162arg	A	-	GLY	GA162	pnca_complex.pdb
388	G162S	LBOV	PZA	G	G162	162	S	NA	Other	OM	hydrophobic	hydrophilic	neutral	neutral	non-polar	polar	8.315	0.821	-0.866	0.905309	-1.14	-0.55	21	63	-0.02	5	5	26	0.25	loop	1.86666666666667	3.93	NA	NA	NA	NA	NA	-6	0	0	0	0	0	0	0	-6	0	-2	-4	0	-3	-13	0.368161434977578	-0.223772609819121	0.0329733243977593	-0.194373401534527	-0.143979057591623	0.221052631578947	-0.0164744645799012	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	141/150	F:S:G:A:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineageBOV	other_mutations_pyrazinamide	SER	SRR5657412	SRR5657412	lineageBOV	mx		pnca_p.gly162ser	A	-	GLY	GA162	pnca_complex.pdb
389	G162V	L3	PZA	G	G162	162	V	NA	XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	non-polar	non-polar	8.315	-0.162	-0.386	2.07181	-1.467	-1.39	66	80	-0.02	5	5	26	0.25	loop	1.86666666666667	3.93	NA	NA	NA	NA	NA	-6	-2	0	-2	6	0	2	2	-10	0	-2	-8	4	3	-19	-0.0410334346504559	-0.0997416020671834	0.075459829981279	-0.250127877237852	-0.363874345549738	0.694736842105263	-0.0164744645799012	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.244	0.083	6:5	141/150	F:S:G:A:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage3	other_mutations_pyrazinamide	VAL	ERR3335739	ERR3335739	lineage3	pk	Pakistan	pnca_p.gly162val	A	-	GLY	GA162	pnca_complex.pdb
390	V163A	L2	PZA	V	V163	163	A	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	6.663	0.052	-0.478	0.471406	-0.834	-0.36	65	80	-1.017	9	9	87	0.5	loop	1	4.02	0.0002156101768003	0.807902480033628	4.5966027178937e-05	0	0.000393645565148	2	0	0	0	0	0	0	0	0	0	0	0	2	6	1	0.0233183856502242	-0.123514211886305	0.0171696326459254	-0.142199488491049	-0.094240837696335	0.684210526315789	-0.837726523887974	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.094	-0.993	9:9	141/150	V:L:I:M	-9.98760797391822	0.930014323968985	113.719344729607	-0.087826816076596	NA	127.94873509896	-0.0926410587047397	1	0	0	11.7369754738005	0.984170607395186	lineage2	other_mutations_pyrazinamide	ALA	DRR185076	DRR185076	lineage2.2.1	vn	Vietnam	pnca_p.val163ala	A	S	VAL	VA163	pnca_complex.pdb
391	V163G	L3	PZA	V	V163	163	G	0	MDR	OM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	6.663	0.031	-0.815	1.14986	-1.019	-0.88	75	85	-1.017	9	9	87	0.5	loop	1	4.02	0.0001437401178668	1.2119588062211	4.59700131334254e-05	0	1.01056822389159e-25	2	0	0	0	2	0	0	0	0	-1	-1	2	6	11	13	0.0139013452914798	-0.210594315245478	0.0418804041404731	-0.173742540494459	-0.230366492146597	0.789473684210526	-0.837726523887974	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-1.094	-0.993	9:9	141/150	V:L:I:M	-9.98752126245061	0.942832958100734	139.277183325398	-0.0717096729269461	NA	144471.706314857	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage3	other_mutations_pyrazinamide	GLY	ERR2510439	ERR2510439	lineage3	pk	Pakistan	pnca_p.val163gly	A	S	VAL	VA163	pnca_complex.pdb
392	V163L	L4	PZA	V	V163	163	L	0	Sensitive	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	6.663	0.072	-0.098	-0.813595	-0.458	-0.37	67	80	-1.017	9	9	87	0.5	loop	1	4.02	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-20	0	0	0	0	0	0	0	-4	0	0	-4	-6	0	-4	0.032286995515695	-0.0253229974160206	-0.156424478966392	-0.0780903665814152	-0.0968586387434555	0.705263157894737	-0.837726523887974	Stabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-1.094	-0.993	9:9	141/150	V:L:I:M	-8.98740878149642	0.940032862253634	119.468044435493	-0.0752285585987741	NA	4692673.5108124	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	LEU	ERR2515073	ERR2515073	lineage4.1.1.3	gb	UK	pnca_p.val163leu	A	S	VAL	VA163	pnca_complex.pdb
393	S164P	L4	PZA	S	S164	164	P	NA	Pre-XDR	DM	hydrophilic	hydrophilic	neutral	non-polar	polar	non-polar	10.588	-0.348	-0.304	4.82419	-1.323	-0.03	-23	61	-0.033	5	5	46	0.296774193548387	loop	1.73333333333333	3.92	0.0004312203536006	58.3312262958281	1393450.9402227	Inf	23.547488635408	0	0	0	0	4	0	4	0	-4	2	-2	-6	-2	0	-2	-0.0881458966565349	-0.0785529715762273	0.175707500783077	-0.225575447570332	-0.0078534031413612	-0.247311827956989	-0.0271828665568369	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.244	0.083	6:5	140/150	N:V:Q:H:F:E:D:T:G:A:S	14.1472939191292	0.915018435775013	132.576060676288	0.106710773023138	4.17896182047499	NA	1.7659011066874	2.48532104922106e-05	4.60461750194108	5.71767556863798	Inf	1.21868387173808e-06	lineage4	dr_mutations_pyrazinamide	PRO	ERR2516576	ERR2516576	lineage4	pe	Peru	pnca_p.ser164pro	A	-	SER	SA164	pnca_complex.pdb
394	T167I	L2	PZA	T	T167	167	I	1	Pre-XDR	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	10.663	-0.489	0.627	0.429573	-0.058	-0.04	19	59	0.303	4	4	57	0.331395348837209	helix	-1.63333333333333	4.06	0.0001437401178668	4.85029436501262	4.85029432717736	4.84956447904112	0.0881192621863332	-6	0	0	0	2	0	0	2	-3	0	1	-2	0	0	-2	-0.123860182370821	0.533163265306122	0.0156459837265714	-0.00988917306052856	-0.0104712041884816	0.2	0.0971153846153846	Destabilising	Stabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	129/150	L:N:R:E:Q:D:G:A:S:T:P	1.57903938912402	0.264247566376722	1.41439286276584	1.11640791656443	0.191756076673841	122.683760399237	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	other_mutations_pyrazinamide	ILE	ERS458430,SAMEA2535028	ERR552955_ERR552956	lineage2.2.1	uz	Nukus	pnca_p.thr167ile	A	H	THR	TA167	pnca_complex.pdb
395	T168I	L1	PZA	T	T168	168	I	NA	Sensitive	OM	hydrophobic	hydrophobic	neutral	non-polar	polar	non-polar	10.468	-1.201	0.963	-0.7812	-0.018	0.69	11	59	0.703	2	2	24	0.13953488372093	helix	0.933333333333333	4.77	0.0002874802357337	0.605874316939891	1.69083297052993e-05	0	0.0596751347828363	-12	0	0	0	2	0	0	2	-3	0	-3	-2	0	0	0	-0.304204660587639	0.818877551020408	-0.150196108590325	-0.0030690537084399	0.387640449438202	0.115789473684211	0.225320512820513	Destabilising	Stabilising	Stabilising	Destabilising	Stabilising	effect	0.389	0.842	4:2	124/150	L:N:V:C:R:Y:H:F:W:G:S:A:T:P	-10.9877041754913	0.946048325624975	162.371849597271	-0.0676700068561389	NA	2065.08997177458	-0.217617456890647	1	0	0	7.34660540805975	0.807010141806273	lineage1	other_mutations_pyrazinamide	ILE	ERR768038	ERR768038	lineage1.1	th	Thailand	pnca_p.thr168ile	A	H	THR	TA168	pnca_complex.pdb
396	T168N	L4	PZA	T	T168	168	N	NA	MDR	DM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	10.468	-0.341	-0.505	0.148032	-1.149	-0.45	3	53	0.703	2	2	24	0.13953488372093	helix	0.933333333333333	4.77	NA	NA	NA	NA	NA	-10	0	0	0	0	0	0	0	-1	0	1	-2	-2	0	2	-0.0863728470111449	-0.130490956072351	0.00539164766643114	-0.195907928388747	-0.117801047120419	0.0315789473684211	0.225320512820513	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.389	0.842	4:2	124/150	L:N:V:C:R:Y:H:F:W:G:S:A:T:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	ASN	ERR1035323	ERR1035323	lineage4.3.3	br	Brazil	pnca_p.thr168asn	A	H	THR	TA168	pnca_complex.pdb
397	T168P	L2	PZA	T	T168	168	P	NA	Pre-XDR	DM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	10.468	-1.31	0.151	3.22365	-1.21	0.61	49	71	0.703	2	2	24	0.13953488372093	helix	0.933333333333333	4.77	NA	NA	NA	NA	NA	0	-2	0	-2	4	2	0	2	0	4	0	-4	2	2	-2	-0.331813576494427	0.128401360544218	0.117412350031687	-0.206308610400682	0.342696629213483	0.515789473684211	0.225320512820513	Destabilising	Stabilising	Destabilising	Destabilising	Stabilising	effect	0.389	0.842	4:2	124/150	L:N:V:C:R:Y:H:F:W:G:S:A:T:P	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	dr_mutations_pyrazinamide	PRO	SAMN02414858	SRR1162695_SRR1166879_SRR1165549_SRR1165524	lineage2.2.1	se	Sweden	pnca_p.thr168pro	A	H	THR	TA168	pnca_complex.pdb
398	T168S	L3	PZA	T	T168	168	S	1	Pre-XDR	OM	hydrophobic	hydrophilic	neutral	neutral	polar	polar	10.468	-0.83	-0.382	0.47424	-0.28	0.09	-71	87	0.703	2	2	24	0.13953488372093	helix	0.933333333333333	4.77	0.0002874802357337	1.61648444070648	1.61648999729673	1.61641362774286	5.75148528770432e-25	0	0	0	0	0	0	0	0	2	0	2	0	2	2	4	-0.210233029381966	-0.0987080103359173	0.0172728530947924	-0.0477408354646206	0.0505617977528089	-0.763440860215054	0.225320512820513	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	neutral	0.389	0.842	4:2	124/150	L:N:V:C:R:Y:H:F:W:G:S:A:T:P	0.480257130287145	0.677247786834332	1.1538493489906	0.416221693678882	0.0799389646162879	12.6318165284439	0.208571528713835	0.527693668101443	0.277618116975238	0.0307788891197694	20.1401594005	0.999999999999395	lineage3	other_mutations_pyrazinamide	SER	ERR2510560	ERR2510560	lineage3	pk	Pakistan	pnca_p.thr168ser	A	H	THR	TA168	pnca_complex.pdb
399	A171E	L2	PZA	A	A171	171	E	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	acidic	non-polar	neg	10.476	1.116	-2.671	3.52921	-3.951	-0.69	75	85	-0.872	8	8	5	0.0387596899224806	helix	2.46666666666667	5.33	0.0001437401178668	1.2119588062211	4.59700131334266e-05	0	1.01056822389159e-25	-36	-26	0	-26	0	0	0	0	-1	0	-3	-3	0	0	-5	0.500448430493273	-0.690180878552972	0.128541510354825	-0.673657289002558	-0.180628272251309	0.789473684210526	-0.71828665568369	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	122/150	R:C:V:T:E:A	-9.98752126245058	0.942832958100734	139.277183325398	-0.0717096729269461	NA	144471.706314849	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	dr_mutations_pyrazinamide	GLU	SAMN02584620	SRR1169146_SRR1166097	lineage2.2.1	md	Moldova	pnca_p.ala171glu	A	H	ALA	AA171	pnca_complex.pdb
400	A171P	L4	PZA	A	A171	171	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	10.476	-1.239	-1.241	4.26761	-3.012	-0.05	80	91	-0.872	8	8	5	0.0387596899224806	helix	2.46666666666667	5.33	NA	NA	NA	NA	NA	-2	0	0	0	4	2	0	0	-3	4	-5	-4	4	2	1	-0.313829787234043	-0.320671834625323	0.155435645655927	-0.513554987212276	-0.013089005235602	0.842105263157895	-0.71828665568369	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	122/150	R:C:V:T:E:A	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	dr_mutations_pyrazinamide	PRO	ERR1213889	ERR1213889	lineage4.3.4.2.1	rw	Rwanda	pnca_p.ala171pro	A	H	ALA	AA171	pnca_complex.pdb
401	A171T	L3	PZA	A	A171	171	T	0	MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	10.476	0.043	-1.816	0.486585	-1.914	-1.47	25	63	-0.872	8	8	5	0.0387596899224806	helix	2.46666666666667	5.33	0.0003593502946672	0.484657419083649	1.69068633684891e-05	0	0.177777263899814	-2	0	0	0	0	0	-2	0	-3	0	-1	-2	0	0	-5	0.0192825112107623	-0.469250645994832	0.0177224848665856	-0.326342710997442	-0.384816753926702	0.263157894736842	-0.71828665568369	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	122/150	R:C:V:T:E:A	-10.9877909019996	0.93969121942854	145.229797681913	-0.075657964669657	NA	60.3389082676185	-0.314565134742567	0.596111875058711	0.224672226566594	0	5.29224350433385	0.673290424524484	lineage3	dr_mutations_pyrazinamide	THR	ERR2510582	ERR2510582	lineage3	pk	Pakistan	pnca_p.ala171thr	A	H	ALA	AA171	pnca_complex.pdb
402	A171V	L2	PZA	A	A171	171	V	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	10.476	-1.298	-0.659	0.499158	-1.528	-0.62	38	66	-0.872	8	8	5	0.0387596899224806	helix	2.46666666666667	5.33	0.0001437401178668	4.85029436501262	4.85029432717736	4.84956447904112	0.0881192621863332	-2	0	0	0	0	0	0	0	-7	0	-5	-6	2	2	-1	-0.328774062816616	-0.170284237726098	0.0181804208946743	-0.260528559249787	-0.162303664921466	0.4	-0.71828665568369	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.993	-0.827	9:8	122/150	R:C:V:T:E:A	1.57903938912402	0.264247566376722	1.41439286276584	1.11640791656443	0.191756076673654	122.683760399096	0.685768096792206	0.312734154310187	0.504824686014995	0.061763864579114	379.558337204203	0.766581879696361	lineage2	dr_mutations_pyrazinamide	VAL	ERR502955	ERR502955	lineage2.2.1			pnca_p.ala171val	A	H	ALA	AA171	pnca_complex.pdb
403	L172P	L4	PZA	L	L172	172	P	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	13.187	-1.353	-1.821	4.7117	-2.793	-1.44	79	85	1.192	1	1	51	0.253731343283582	helix	0.7	4.4	0.0007187005893344	43.8	43.7999992563294	43.791882510185	32.5514826715601	14	0	0	0	2	2	0	0	3	4	1	-1	2	-2	4	-0.342705167173252	-0.470542635658915	0.17161037012216	-0.476214833759591	-0.37696335078534	0.831578947368421	0.382051282051282	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	0.59	1.183	3:1	122/150	H:F:E:L:V:K:R:C:I:T:A:M:D:W	3.77963380040362	0.000336884092116	1.0542469487651	3.58515033392406	8.22614212186537	807.950983717549	1.6414741105041	1.04401171857701e-06	5.98129462653992	6.06119169154248	1899.94618136838	1.16076545932747e-08	lineage4	dr_mutations_pyrazinamide	PRO	ERR751403	ERR751403	lineage4.3.3	pe		pnca_p.leu172pro	A	H	LEU	LA172	pnca_complex.pdb
404	L172R	L4	PZA	L	L172	172	R	NA	MDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	13.187	-0.704	-0.848	-0.385063	-1.797	-0.43	33	66	1.192	1	1	51	0.253731343283582	helix	0.7	4.4	0.0001437401178668	19.411022297013	511754.463552565	Inf	4.73122070382274	-32	-36	-2	-34	-2	0	0	-4	-8	0	-1	-6	-8	-8	0	-0.178318135764944	-0.219121447028424	-0.0740334922710144	-0.306393861892583	-0.112565445026178	0.347368421052632	0.382051282051282	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	0.59	1.183	3:1	122/150	H:F:E:L:V:K:R:C:I:T:A:M:D:W	13.1456002256205	0.924803768446996	139.277183326561	0.0943844491369283	0.0001628371344453	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage4	dr_mutations_pyrazinamide	ARG	ERR1035063	ERR1035063	lineage4.3.2.1	za	South Africa	pnca_p.leu172arg	A	H	LEU	LA172	pnca_complex.pdb
405	E173G	L4	PZA	E	E173	173	G	NA	Sensitive	OM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	15.936	-0.78	-1.172	0.769667	-0.869	-1.04	-5	53	1.516	1	1	114	0.511210762331839	helix	-1.06666666666667	3.82	NA	NA	NA	NA	NA	6	18	0	18	0	0	0	0	3	0	3	1	0	3	13	-0.197568389057751	-0.302842377260982	0.0280329475010745	-0.148167092924126	-0.272251308900524	-0.0537634408602151	0.485897435897436	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	0.842	1.712	2:1	122/150	Q:E:F:H:K:R:L:V:S:M:G:A:T:D	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	GLY	ERR126622	ERR126622	lineage4.3.4.2.1	mw	Malawi	pnca_p.glu173gly	A	H	GLU	EA173	pnca_complex.pdb
406	E174D	L2	PZA	E	E174	174	D	1	MDR	OM	hydrophilic	hydrophilic	acidic	acidic	neg	neg	14.835	-0.016	-0.698	0.557649	-0.445	-0.43	-14	57	-0.292	6	6	104	0.466367713004484	helix	-1.7	3.93	7.18700589334483e-05	9.70142977291842	188179.926264691	Inf	0.763784339928386	14	0	0	0	0	0	0	0	2	0	2	0	0	0	0	-0.0040526849037487	-0.180361757105943	0.0203107904340795	-0.0758738277919864	-0.112565445026178	-0.150537634408602	-0.240527182866557	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.485	-0.147	7:6	122/150	D:T:A:G:S:K:R:Q:E	12.1451538387268	0.919026337424243	119.468044436249	0.101660271548244	5.01153666061119e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage2	other_mutations_pyrazinamide	ASP	ERS2592796	ERR2677451	lineage2.2.1	de	Germany	pnca_p.glu174asp	A	H	GLU	EA174	pnca_complex.pdb
407	E174G	L2	PZA	E	E174	174	G	0	MDR	DM	hydrophilic	hydrophobic	acidic	neutral	neg	non-polar	14.835	-1.181	-0.868	0.834042	-0.968	-0.01	54	75	-0.292	6	6	104	0.466367713004484	helix	-1.7	3.93	0.0001437401178668	1.2119588062211	4.59700131334288e-05	0	1.01056822389159e-25	22	22	0	22	0	0	0	0	2	0	2	0	0	3	15	-0.299138804457953	-0.224289405684755	0.0303776251283882	-0.165046888320546	-0.0026178010471204	0.568421052631579	-0.240527182866557	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.485	-0.147	7:6	122/150	D:T:A:G:S:K:R:Q:E	-9.98752126245053	0.942832958100734	139.277183325396	-0.0717096729269466	NA	144471.706314838	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	dr_mutations_pyrazinamide	GLY	MDR_11151	SRR6824687	lineage2.2.1	cn	China: Luqiao District, Taizhou City, Zhejiang Province	pnca_p.glu174gly	A	H	GLU	EA174	pnca_complex.pdb
408	E174K	L1	PZA	E	E174	174	K	NA	Sensitive	OM	hydrophilic	hydrophobic	acidic	basic	neg	pos	14.835	-0.819	0.204	-0.813418	-0.452	-0.16	26	63	-0.292	6	6	104	0.466367713004484	helix	-1.7	3.93	NA	NA	NA	NA	NA	-6	4	0	4	0	0	2	-2	0	0	0	-1	0	0	0	-0.207446808510638	0.173469387755102	-0.156390448358071	-0.0770673486786019	-0.0418848167539267	0.273684210526316	-0.240527182866557	Destabilising	Stabilising	Stabilising	Destabilising	Destabilising	effect	-0.485	-0.147	7:6	122/150	D:T:A:G:S:K:R:Q:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage1	other_mutations_pyrazinamide	LYS	SRR5067256	SRR5067256	lineage1.1.1	vn	Vietnam	pnca_p.glu174lys	A	H	GLU	EA174	pnca_complex.pdb
409	M175I	L2	PZA	M	M175	175	I	NA	Pre-XDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.016	-1.143	-0.676	1.67797	-1.272	-0.65	19	59	-0.521	7	7	0	0	helix	-2.03333333333333	6.81	0.0002874802357337	1.61648444070648	1.61648999729673	1.61641362774286	5.75148528770432e-25	14	2	0	2	0	0	0	0	-5	0	-1	-4	6	4	4	-0.28951367781155	-0.174677002583979	0.0611153198959783	-0.216879795396419	-0.170157068062827	0.2	-0.429159802306425	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	120/150	F:M:I:W:C:L:V	0.480257130287143	0.677247786834333	1.1538493489906	0.416221693678881	0.0799389646162787	12.6318165284426	0.208571528713835	0.527693668101443	0.277618116975238	0.0307788891197694	20.1401594005	0.999999999999395	lineage2	dr_mutations_pyrazinamide	ILE	ERR2516249	ERR2516249	lineage2.2.1	be	Belgium	pnca_p.met175ile	A	H	MET	MA175	pnca_complex.pdb
410	M175K	L4	PZA	M	M175	175	K	1	MDR	DM	hydrophobic	hydrophobic	non-polar	basic	non-polar	pos	11.016	-0.207	-1.311	2.59826	-4.252	-0.95	78	85	-0.521	7	7	0	0	helix	-2.03333333333333	6.81	7.18700589334483e-05	9.70142977291842	188179.926264637	Inf	0.763784339928386	-16	-36	0	-36	-2	0	-2	0	-5	0	-4	-1	-2	2	-2	-0.0524316109422492	-0.338759689922481	0.0946342849234042	-0.724978687127025	-0.24869109947644	0.821052631578947	-0.429159802306425	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	120/150	F:M:I:W:C:L:V	12.1451538387265	0.919026337424235	119.468044436234	0.101660271548255	5.01153666062558e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	LYS	ERR040122	ERR040122	lineage4.8	ug	Uganda	pnca_p.met175lys	A	H	MET	MA175	pnca_complex.pdb
411	M175R	L4	PZA	M	M175	175	R	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	basic	non-polar	pos	11.016	-0.698	-1.175	2.37207	-3.407	-0.8	74	85	-0.521	7	7	0	0	helix	-2.03333333333333	6.81	7.18700589334483e-05	9.70142977291842	188179.92626466	Inf	0.763784339928386	-36	-40	-2	-38	-2	0	-2	0	-9	0	-4	-5	-4	-6	0	-0.176798378926039	-0.303617571059431	0.0863959527677212	-0.580903665814152	-0.209424083769634	0.778947368421053	-0.429159802306425	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	120/150	F:M:I:W:C:L:V	12.1451538387266	0.919026337424239	119.46804443624	0.10166027154825	5.01153666061918e-06	NA	0.986835744238421	0.170978870202674	0.767057556984365	0.124324963210678	Inf	0.382146811758261	lineage4	dr_mutations_pyrazinamide	ARG	SAMN02628523	SRR1172252_SRR1180184_SRR1183085_SRR1172151	lineage4.8	ir	Iran	pnca_p.met175arg	A	H	MET	MA175	pnca_complex.pdb
412	M175T	L1	PZA	M	M175	175	T	NA	Pre-MDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	polar	11.016	0.116	-2.131	2.83612	-3.057	-1.73	72	85	-0.521	7	7	0	0	helix	-2.03333333333333	6.81	0.0002156101768003	29.1287878787879	511969.848428383	Inf	9.2872640727157	24	0	0	0	-2	0	-2	0	-4	0	-4	0	10	10	8	0.0520179372197309	-0.550645994832041	0.103297663881584	-0.521227621483376	-0.452879581151832	0.757894736842105	-0.429159802306425	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	120/150	F:M:I:W:C:L:V	13.1460210124993	0.907969111549572	113.719344731884	0.115600569485285	0.183926936278602	NA	1.4643224129316	0.0049931328456149	2.3016268790981	2.00455510251839	Inf	0.0023075254069185	lineage1	dr_mutations_pyrazinamide	THR	ERR1213948	ERR1213948	lineage1.2.2	bd	Bangladesh	pnca_p.met175thr	A	H	MET	MA175	pnca_complex.pdb
413	M175V	L4	PZA	M	M175	175	V	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	11.016	-1.081	-1.251	2.746	-2.087	-1.13	52	75	-0.521	7	7	0	0	helix	-2.03333333333333	6.81	0.0007187005893344	11.3440134907251	11.3439859738742	11.3408060961982	16.1999449415698	24	0	0	0	0	0	0	0	-2	0	-4	0	8	8	8	-0.273809523809524	-0.323255813953488	0.100015297314229	-0.355839727195226	-0.295811518324607	0.547368421052632	-0.429159802306425	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	120/150	F:M:I:W:C:L:V	2.42868773335338	0.0004314327258942	0.689954362035746	3.52007011911253	3.15129211999938	52.6672541515188	1.05476673431854	0.000313793551117	3.50335598599778	2.58674701617199	67.9086927550226	5.69957727561539e-05	lineage4	dr_mutations_pyrazinamide	VAL	ERR2707135	ERR2707135	lineage4.3.3	cd	Democratic Republic of Congo	pnca_p.met175val	A	H	MET	MA175	pnca_complex.pdb
414	R176C	L4	PZA	R	R176	176	C	NA	Sensitive	OM	hydrophilic	hydrophobic	basic	neutral	pos	non-polar	17.132	-0.881	-0.417	1.53676	-0.701	0.75	55	75	1.185	1	1	188	0.686131386861314	helix	-1.1	3.67	NA	NA	NA	NA	NA	32	22	0	22	0	0	2	0	0	0	-3	3	2	2	4	-0.223150962512665	-0.107751937984496	0.0559721443192331	-0.119522591645354	0.421348314606741	0.578947368421053	0.379807692307692	Destabilising	Destabilising	Destabilising	Destabilising	Stabilising	effect	0.59	1.183	3:1	116/150	S:M:A:G:T:D:E:Q:N:L:V:K:I:R	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	CYS	ERR1193718	ERR1193718	lineage4.3	id	Indonesia	pnca_p.arg176cys	A	H	ARG	RA176	pnca_complex.pdb
415	T177P	L3	PZA	T	T177	177	P	0	MDR	OM	hydrophobic	hydrophilic	neutral	non-polar	polar	non-polar	19.207	-0.36	-0.045	0.790967	-0.444	-0.19	21	63	1.87	1	1	126	0.732558139534884	helix	-1.13333333333333	3.45	0.0002874802357337	4.85191417753471	4.85191414039982	4.85118443551178	1.17497090855698	0	13	-2	15	2	2	2	0	4	4	-1	0	-2	-4	-2	-0.0911854103343465	-0.0116279069767441	0.0288087398655293	-0.0757033248081841	-0.0497382198952879	0.221052631578947	0.599358974358974	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	1.183	1.712	1:1	114/150	N:L:R:K:H:E:Q:D:T:S:M:G:A	1.57937329521209	0.114342297004304	1.0002536454007	1.57897279602454	0.581889091312676	40.456291107532	0.685913110299906	0.137954936368295	0.860262754777729	0.351471331417763	67.0421060419706	0.278382201819561	lineage3	other_mutations_pyrazinamide	PRO	ERR2510450	ERR2510450	lineage3	pk	Pakistan	pnca_p.thr177pro	A	H	THR	TA177	pnca_complex.pdb
416	A178P	L4	PZA	A	A178	178	P	NA	MDR	OM	hydrophobic	hydrophilic	non-polar	non-polar	non-polar	non-polar	17.128	-0.05	-0.485	2.01949	-1.803	-0.09	60	80	-0.496	7	7	32	0.248062015503876	loop	0.1	3.71	NA	NA	NA	NA	NA	0	-2	0	-2	2	2	2	0	-6	2	-8	-2	0	-2	-5	-0.0126646403242147	-0.125322997416021	0.0735542216945053	-0.307416879795396	-0.0235602094240837	0.631578947368421	-0.408566721581549	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	110/150	G:M:A:S:C:K:V:Q:H:E	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	PRO	ERR1213826	ERR1213826	lineage4.3.4.2.1	rw	Rwanda	pnca_p.ala178pro	A	T	ALA	AA178	pnca_complex.pdb
417	S179I	L2	PZA	S	S179	179	I	0	Sensitive	OM	hydrophilic	hydrophobic	neutral	non-polar	polar	non-polar	19.449	-0.451	0.381	1.25588	0.395	-0.66	35	66	-0.876	8	8	87	0.561290322580645	loop	1.73333333333333	3.5	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-8	0	0	0	2	0	2	0	0	0	0	0	0	0	2	-0.114235055724417	0.323979591836735	0.0457418833179146	0.333614864864865	-0.172774869109948	0.368421052631579	-0.72158154859967	Destabilising	Stabilising	Destabilising	Stabilising	Destabilising	effect	-1.028	-0.779	9:8	107/150	W:R:D:Q:S:G	-8.98740878149641	0.940032862253634	119.468044435492	-0.0752285585987746	NA	4692673.510814	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage2	other_mutations_pyrazinamide	ILE	ERR2514301	ERR2514301	lineage2.2.1	gb	UK	pnca_p.ser179ile	A	T	SER	SA179	pnca_complex.pdb
418	V180A	L2	PZA	V	V180	180	A	1	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.436	-1.661	-2.048	2.8049	-2.408	-1.98	-8	53	-0.509	7	7	6	0.0344827586206896	loop	-0.0333333333333332	5.26	0.0001437401178668	19.411022297013	511754.463552312	Inf	4.73122070382274	2	0	0	0	0	0	0	0	-2	0	-4	0	2	2	5	-0.420719351570415	-0.529198966408269	0.102160563523918	-0.410571184995737	-0.518324607329843	-0.0860215053763441	-0.419275123558484	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	neutral	-0.674	-0.411	7:7	102/150	I:L:V:S:A:G	13.14560022562	0.92480376844698	139.277183326525	0.094384449136949	0.0001628371344461	NA	1.28804840845689	0.0292235860951597	1.53426649183163	0.91081598550184	Inf	0.0296198560706035	lineage2	other_mutations_pyrazinamide	ALA	ERR552136	ERR552136	lineage2.2.1	ru	Russia	pnca_p.val180ala	A	-	VAL	VA180	pnca_complex.pdb
419	V180F	L1	PZA	V	V180	180	F	NA	MDR	DM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.436	-1.073	-1.808	10.5926	-3.033	-1.23	57	75	-0.509	7	7	6	0.0344827586206896	loop	-0.0333333333333332	5.26	0.0005749604714675	14.5802781289507	14.5802277940117	14.5801759034061	15.0663448164144	-22	0	0	0	0	0	0	0	-10	0	-6	-8	-12	-12	-4	-0.271783181357649	-0.4671834625323	0.385805549282847	-0.517135549872123	-0.321989528795811	0.6	-0.419275123558484	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	102/150	I:L:V:S:A:G	2.6796663501904	0.0010245352377103	0.816050632122415	3.28370108999367	3.35697961122208	99.5538692592325	1.16376580853041	0.0005075351447614	3.29453387923245	2.60435121163486	147.676327006136	0.0001037975122989	lineage1	dr_mutations_pyrazinamide	PHE	SRR8380946	SRR8380946	lineage1.1.2	in	India	pnca_p.val180phe	A	-	VAL	VA180	pnca_complex.pdb
420	V180G	L2	PZA	V	V180	180	G	1	XDR	DM	hydrophobic	hydrophobic	non-polar	neutral	non-polar	non-polar	15.436	-1.577	-2.785	4.93179	-3.447	-2.32	61	80	-0.509	7	7	6	0.0344827586206896	loop	-0.0333333333333332	5.26	0.0007905706482679	107.166385135135	3795803.48339175	Inf	47.6847963834956	12	0	0	0	0	0	0	0	-4	0	-6	0	6	13	23	-0.399442755825735	-0.719638242894057	0.17962652699976	-0.58772378516624	-0.607329842931937	0.642105263157895	-0.419275123558484	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	102/150	I:L:V:S:A:G	15.1494066679809	0.92523341791644	161.432542879438	0.0938435732830823	24.1993753459118	NA	2.03005858162844	3.58120158191569e-09	8.44597123233921	12.2081619622864	Inf	5.00558510128865e-12	lineage2	dr_mutations_pyrazinamide	GLY	ERR229982,2395E-sc-2012-10-11T13:40:00Z-1494301	ERR229982	lineage2.2.1	ru	Russia,Samara	pnca_p.val180gly	A	-	VAL	VA180	pnca_complex.pdb
421	V180L	L2	PZA	V	V180	180	L	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.436	-1.664	-0.877	-0.0108685	-2.269	-0.8	56	75	-0.509	7	7	6	0.0344827586206896	loop	-0.0333333333333332	5.26	NA	NA	NA	NA	NA	-16	0	0	0	0	0	0	0	-9	0	-2	-9	-10	-10	-2	-0.421479229989868	-0.226614987080103	-0.00208961393524571	-0.386871270247229	-0.209424083769634	0.589473684210526	-0.419275123558484	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	effect	-0.674	-0.411	7:7	102/150	I:L:V:S:A:G	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage2	other_mutations_pyrazinamide	LEU	1362G-sc-2012-02-21T11:36:33Z-1364711,ERR137219	ERR137219	lineage2.2.1	ru	Russia	pnca_p.val180leu	A	-	VAL	VA180	pnca_complex.pdb
422	L182F	L4	PZA	L	L182	182	F	NA	Pre-XDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.184	-0.294	-1.168	4.99445	-0.804	-1	47	71	0.281	4	4	50	0.248756218905473	strand	1.5	4.17	7.18700589334483e-05	2.42412778478352	0.0001249735077034	0	1.03410070803344e-29	-6	-2	0	-2	0	0	0	0	1	0	1	1	2	4	0	-0.0744680851063829	-0.301808785529716	0.181908740593973	-0.137084398976982	-0.261780104712042	0.494736842105263	0.0900641025641026	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	87/150	F:Q:L:V:R:I	-8.98740878149642	0.940032862253634	119.468044435493	-0.0752285585987741	NA	4692673.5108132	0.384555509373631	1	0	0	188.544222231268	0.999999999999997	lineage4	other_mutations_pyrazinamide	PHE	ERR2510225	ERR2510225	lineage4.3.4.2	pe	Peru	pnca_p.leu182phe	A	E	LEU	LA182	pnca_complex.pdb
423	L182S	L2	PZA	L	L182	182	S	NA	Pre-XDR	DM	hydrophobic	hydrophilic	non-polar	neutral	non-polar	polar	15.184	0.38	-2.349	5.37676	-1.864	-1.98	71	85	0.281	4	4	50	0.248756218905473	strand	1.5	4.17	0.0007187005893344	4.85678180286436	4.85678176777767	4.85605251831648	5.49640065724654	22	0	0	0	0	0	0	0	-1	0	-1	0	4	4	2	0.170403587443946	-0.606976744186047	0.195833302981519	-0.317817561807332	-0.518324607329843	0.747368421052632	0.0900641025641026	Stabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	87/150	F:Q:L:V:R:I	1.58037603086922	0.0125175169650379	0.632856943325042	2.49720896252777	1.34977418228528	17.4757677148671	0.686348592676127	0.0171758041796076	1.76508291991092	1.11658950345066	21.1173440246382	0.0190556572462216	lineage2	dr_mutations_pyrazinamide	SER	ERR2516229	ERR2516229	lineage2.2.1	be	Belgium	pnca_p.leu182ser	A	E	LEU	LA182	pnca_complex.pdb
424	L182W	L4	PZA	L	L182	182	W	NA	MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	15.184	-0.228	-1.318	4.86413	-1.198	-1.15	75	85	0.281	4	4	50	0.248756218905473	strand	1.5	4.17	NA	NA	NA	NA	NA	-20	0	0	0	0	0	0	0	-1	0	-1	0	4	4	2	-0.0577507598784194	-0.340568475452196	0.177162202521872	-0.204262574595055	-0.301047120418848	0.789473684210526	0.0900641025641026	Destabilising	Destabilising	Destabilising	Destabilising	Destabilising	effect	-0.038	0.389	5:4	87/150	F:Q:L:V:R:I	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	lineage4	other_mutations_pyrazinamide	TRP	ERR1034735	ERR1034735	lineage4.3.3	pe	Peru	pnca_p.leu182trp	A	E	LEU	LA182	pnca_complex.pdb
425	V183L	L2	PZA	V	V183	183	L	NA	Pre-MDR	OM	hydrophobic	hydrophobic	non-polar	non-polar	non-polar	non-polar	17.921	-0.108	-0.423	-0.102908	-0.776	-0.67	-6	53	0.4	4	4	34	0.195402298850575	strand	3.5	4.1	0.0001437401178668	1.2119588062211	4.59700131334244e-05	0	1.01056822389159e-25	-10	0	0	0	0	0	0	0	-3	0	-1	-2	-2	0	-4	-0.0273556231003039	-0.109302325581395	-0.0197854341305852	-0.13231031543052	-0.175392670157068	-0.0645161290322581	0.128205128205128	Destabilising	Destabilising	Stabilising	Destabilising	Destabilising	neutral	0.083	0.59	5:3	59/150	L:V:I:R:C:T:A:G	-9.98752126245063	0.942832958100734	139.277183325399	-0.0717096729269459	NA	144471.706314865	0.0834878586628579	1	0	0	25.8386585962163	0.999999999999746	lineage2	other_mutations_pyrazinamide	LEU	ERR751934	ERR751934	lineage2.2.1	th	Thailand	pnca_p.val183leu	A	E	VAL	VA183	pnca_complex.pdb

386 KiB Raw Blame History

386 KiB

Raw Blame History