diff --git a/stats_check.R b/stats_check.R
new file mode 100755
index 0000000..4bc5f5e
--- /dev/null
+++ b/stats_check.R
@@ -0,0 +1,85 @@
+#!/usr/bin/Rscript
+############################################################
+# TASK: to check unpaired stats from WF data
+############################################################
+#=============
+# Input
+#=============
+source("data_extraction_formatting.R")
+#%%========================================================
+# data assignment for stats
+wf_npa = npa_df_adults_clean[npa_df_adults_clean$flustat == 1,]
+wf_sam = sam_df_adults_clean[sam_df_adults_clean$flustat == 1,]
+wf_serum = serum_df_adults_clean[serum_df_adults_clean$flustat == 1,]
+############################################################
+#=========
+# t1
+#=========
+wilcox.test(wf_npa$eotaxin3_npa1[wf_npa$obesity == 1], wf_npa$eotaxin3_npa1[wf_npa$obesity == 0], paired = F)
+length(wf_npa$eotaxin3_npa1) - sum(is.na(wf_npa$eotaxin3_npa1))
+
+wilcox.test(wf_npa$il12p70_npa1[wf_npa$obesity == 1], wf_npa$il12p70_npa1[wf_npa$obesity == 0], paired = F)
+length(wf_npa$il12p70_npa1) - sum(is.na(wf_npa$il12p70_npa1))
+
+#=========
+# t2
+#=========
+wilcox.test(wf_npa$il4_npa2[wf_npa$obesity == 1], wf_npa$il4_npa2[wf_npa$obesity == 0], paired = F)
+length(wf_npa$il4_npa2) - sum(is.na(wf_npa$il4_npa2))
+
+#=========
+# t3
+#=========
+wilcox.test(wf_npa$ip10_npa3[wf_npa$obesity == 1], wf_npa$ip10_npa3[wf_npa$obesity == 0], paired = F)
+length(wf_npa$ip10_npa3) - sum(is.na(wf_npa$ip10_npa3))
+###################################################################
+##########
+# sam
+##########
+#=========
+# t1
+#=========
+wilcox.test(wf_sam$eotaxin3_sam1[wf_sam$obesity == 1], wf_sam$eotaxin3_sam1[wf_sam$obesity == 0], paired = F)
+length(wf_sam$eotaxin3_sam1) - sum(is.na(wf_sam$eotaxin3_sam1))
+
+wilcox.test(wf_sam$il12p70_sam1[wf_sam$obesity == 1], wf_sam$il12p70_sam1[wf_sam$obesity == 0], paired = F)
+length(wf_sam$il12p70_sam1) - sum(is.na(wf_sam$il12p70_sam1))
+
+#=========
+# t2
+#=========
+wilcox.test(wf_sam$il4_sam2[wf_sam$obesity == 1], wf_sam$il4_sam2[wf_sam$obesity == 0], paired = F)
+length(wf_sam$il4_sam2) - sum(is.na(wf_sam$il4_sam2))
+
+#=========
+# t3
+#=========
+wilcox.test(wf_sam$tnfr1_sam3[wf_sam$obesity == 1], wf_sam$tnfr1_sam3[wf_sam$obesity == 0], paired = F)
+length(wf_sam$tnfr1_sam3) - sum(is.na(wf_sam$tnfr1_sam3))
+
+###################################################################
+##########
+# serum
+##########
+
+#=========
+# t1
+#=========
+wilcox.test(wf_serum$eotaxin3_serum1[wf_serum$obesity == 1], wf_serum$eotaxin3_serum1[wf_serum$obesity == 0], paired = F)
+length(wf_serum$eotaxin3_serum1) - sum(is.na(wf_serum$eotaxin3_serum1))
+
+wilcox.test(wf_serum$ifna2a_serum1[wf_serum$obesity == 1], wf_serum$ifna2a_serum1[wf_serum$obesity == 0], paired = F)
+length(wf_serum$ifna2a_serum1) - sum(is.na(wf_serum$ifna2a_serum1))
+
+#=========
+# t2
+#=========
+wilcox.test(wf_serum$il5_serum2[wf_serum$obesity == 1], wf_serum$il5_serum2[wf_serum$obesity == 0], paired = F)
+length(wf_serum$il5_serum2) - sum(is.na(wf_serum$il5_serum2))
+
+#=========
+# t3
+#=========
+wilcox.test(wf_serum$rantes_serum3[wf_serum$obesity == 1], wf_serum$rantes_serum3[wf_serum$obesity == 0], paired = F)
+length(wf_serum$rantes_serum3) - sum(is.na(wf_serum$rantes_serum3))
+