365 lines
11 KiB
R
Executable file
365 lines
11 KiB
R
Executable file
#!/usr/bin/env Rscript
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#require('compare')
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require('getopt', quietly=TRUE) # We need to be able to parse arguments
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#########################################################
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# TASK: To calculate Allele Frequency and
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# Odds Ratio from master data
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# and add the calculated params to meta_data extracted from
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# data_extraction.py
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#########################################################
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#getwd()
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setwd('~/git/LSHTM_analysis/scripts')
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cat(c(getwd(),'\n'))
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# Command line args
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spec = matrix(c(
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"drug" , "d", 1, "character",
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"gene" , "g", 1, "character"
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), byrow = TRUE, ncol = 4)
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opt = getopt(spec);
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drug = opt$drug
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gene = opt$gene
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if(is.null(drug)|is.null(gene)) {
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stop('Missing arguments: --drug and --gene must both be specified (case-sensitive)')
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}
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#options(scipen = 999) #disabling scientific notation in R.
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#%% variable assignment: input and output paths & filenames
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gene_match = paste0(gene,'_p.')
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cat(gene_match)
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#=============
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# directories
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#=============
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datadir = paste0('~/git/Data')
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indir = paste0(datadir, '/', drug, '/', 'input')
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outdir = paste0(datadir, '/', drug, '/', 'output')
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#===========
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# input
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#===========
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# input file 1: master data
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#in_filename_master = 'original_tanushree_data_v2.csv' #19K
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in_filename_master = 'mtb_gwas_meta_v3.csv' #33k
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infile_master = paste0(datadir, '/', in_filename_master)
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cat(paste0('Reading infile1: raw data', ' ', infile_master) )
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# input file 2: gene associated meta data file to extract valid snps and add calcs to.
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# This is outfile_metadata from data_extraction.py
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in_filename_metadata = paste0(tolower(gene), '_metadata.csv')
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infile_metadata = paste0(outdir, '/', in_filename_metadata)
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cat(paste0('Reading input file 2 i.e gene associated metadata:', infile_metadata))
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#===========
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# output
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#===========
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#out_filename_af_or = paste0(tolower(gene), '_meta_data_with_AF_OR.csv')
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out_filename_af_or = paste0(tolower(gene), '_af_or.csv')
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outfile_af_or = paste0(outdir, '/', out_filename_af_or)
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cat(paste0('Output file with full path:', outfile_af_or))
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#%% end of variable assignment for input and output files
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#########################################################
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# 1: Read master/raw data stored in Data/
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#########################################################
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raw_data_all = read.csv(infile_master, stringsAsFactors = F)
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# building cols to extract
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dr_muts_col = paste0('dr_mutations_', drug)
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other_muts_col = paste0('other_mutations_', drug)
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cat('Extracting columns based on variables:\n'
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, drug
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, '\n'
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, dr_muts_col
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, '\n'
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, other_muts_col
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, '\n===============================================================')
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raw_data = raw_data_all[,c("id"
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, drug
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, dr_muts_col
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, other_muts_col)]
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rm(raw_data_all)
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rm(indir, in_filename_master, infile_master)
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#===========
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# 1a: exclude na
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#===========
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raw_data = raw_data[!is.na(raw_data[[drug]]),]
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total_samples = length(unique(raw_data$id))
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cat(paste0('Total samples without NA in', ' ', drug, 'is:', total_samples))
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# sanity check: should be true
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is.numeric(total_samples)
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#===========
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# 1b: combine the two mutation columns
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#===========
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all_muts_colname = paste0('all_mutations_', drug)
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print(paste('New column added:', all_muts_colname))
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#raw_data$all_mutations_pyrazinamide = paste(raw_data$dr_mutations_pyrazinamide, raw_data$other_mutations_pyrazinamide)
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raw_data[[all_muts_colname]] = paste(raw_data[[dr_muts_col]], raw_data[[other_muts_col]])
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head(raw_data[[all_muts_colname]])
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#===========
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# 1c: create yet another column that contains all the mutations but in lower case
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#===========
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head(raw_data[[all_muts_colname]])
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raw_data$all_muts_gene = tolower(raw_data[[all_muts_colname]])
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head(raw_data$all_muts_gene)
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# sanity checks
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#table(grepl("gene_p",raw_data$all_muts_gene))
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cat(paste0('converting gene match:', gene_match, ' ', 'to lowercase'))
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gene_match = tolower(gene_match)
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table(grepl(gene_match,raw_data$all_muts_gene))
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# sanity check: should be TRUE
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#sum(table(grepl("gene_p",raw_data$all_muts_gene))) == total_samples
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# sanity check
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if(sum(table(grepl(gene_match, raw_data$all_muts_gene))) == total_samples){
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cat('PASS: Total no. of samples match')
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} else{
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cat('FAIL: No. of samples mismatch')
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}
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#########################################################
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# 2: Read valid snps for which OR
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# can be calculated
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#########################################################
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cat(paste0('Reading gene associated metadata:', infile_metadata))
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gene_metadata = read.csv(infile_metadata
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#, file.choose()
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, stringsAsFactors = F
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, header = T)
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# get just the variable name from variable
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#deparse(substitute(myvar)
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print(paste('Dim of', deparse(substitute(gene_metadata)), ':')); print(dim(gene_metadata))
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# clear variables
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rm(in_filename_metadata, infile_metadata)
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# count na in drug column
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tot_pza_na = sum(is.na(gene_metadata[[drug]]))
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expected_rows = nrow(gene_metadata) - tot_pza_na
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# drop na from the drug column
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gene_snps_or = gene_metadata[!is.na(gene_metadata[[drug]]),]
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# sanity check
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if(nrow(gene_snps_or) == expected_rows){
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cat('PASS: no. of rows match with expected_rows')
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} else{
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cat('FAIL: nrows mismatch.')
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}
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# extract unique snps to iterate over for AF and OR calcs
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gene_snps_unique = unique(gene_snps_or$mutation)
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cat(paste0('Total no. of distinct comp snps to perform OR calcs: ', length(gene_snps_unique)))
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#===========================================================================================
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#########################
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# custom chisq function:
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# To calculate OR
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#########################
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#i = "pnca_p.trp68gly"
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i = "pnca_p.gly162asp"
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mut = grepl(i,raw_data$all_muts_gene)
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mut = as.numeric(mut)
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dst = raw_data[[drug]]
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#x = as.numeric(mut)
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#y = dst
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mychisq_or = function(x,y){
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tab = as.matrix(table(x,y))
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a = tab[2,2]
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if (a==0){ a<-0.5}
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b = tab[2,1]
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if (b==0){ b<-0.5}
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c = tab[1,2]
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if (c==0){ c<-0.5}
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d = tab[1,1]
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if (d==0){ d<-0.5}
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(a/b)/(c/d)
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}
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or_mychisq = mychisq_or(dst, mut)
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print(paste0('mychisq OR:', or_mychisq ))
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odds_fisher = fisher.test(table(dst, mut))$estimate
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pval_fisher = fisher.test(table(dst, mut))$p.value
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print(paste0('fisher OR:', odds_fisher))
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print(paste0('fisher p-value:', pval_fisher))
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model<-glm(dst ~ mut, family = binomial)
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or_logistic = exp(summary(model)$coefficients[2,1])
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print(paste0('logistic OR:', or_logistic))
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pval_logistic = summary(model)$coefficients[2,4]
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print(paste0('logistic pval:', pval_logistic))
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#=====================================
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#OR calcs using the following 4
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#1) chisq.test
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#2) fisher
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#3) modified chisq.test
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#4) logistic
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#5) adjusted logistic?
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#6) kinship (separate script)
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#======================================
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# TEST FOR a few muts: sapply and df
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#===============================================
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#snps <- gene_snps_unique[1:2]# reassign so you test with subset of muts
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snps <- gene_snps_unique
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cat(paste0('Running calculations for:', length(snps), ' nssnps\n'
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, 'gene: ', gene
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, '\ndrug: ', drug ))
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# DV: <drug> 0 or 1
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dst = raw_data[[drug]]
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# initialise an empty df
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ors_df = data.frame()
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x = sapply(snps,function(m){
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mut = grepl(m,raw_data$all_muts_gene)
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mut = as.numeric(mut)
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cat(paste0('Running mutation:', m, '\n'))
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model<-glm(dst ~ mut, family = binomial)
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#-------------------
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# allele frequency
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#-------------------
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afs = mean(mut)
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#-------------------
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# logistic model
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#-------------------
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beta_logistic = summary(model)$coefficients[2,1]
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or_logistic = exp(summary(model)$coefficients[2,1])
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#print(paste0('logistic OR:', or_logistic))
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pval_logistic = summary(model)$coefficients[2,4]
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#print(paste0('logistic pval:', pval_logistic))
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se_logistic = summary(model)$coefficients[2,2]
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#print(paste0('logistic SE:', se_logistic))
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zval_logistic = summary(model)$coefficients[2,3]
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#print(paste0('logistic zval:', zval_logistic))
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ci_mod = exp(confint(model))[2,]
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#print(paste0('logistic CI:', ci_mod))
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ci_lower_logistic = ci_mod[["2.5 %"]]
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ci_upper_logistic = ci_mod[["97.5 %"]]
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#-------------------
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# custom_chisq and fisher: OR p-value and CI
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#-------------------
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or_mychisq = mychisq_or(dst, mut)
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#print(paste0('mychisq OR:', or_mychisq))
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odds_fisher = fisher.test(table(dst, mut))$estimate
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or_fisher = odds_fisher[[1]]
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pval_fisher = fisher.test(table(dst, mut))$p.value
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ci_lower_fisher = fisher.test(table(dst, mut))$conf.int[1]
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ci_upper_fisher = fisher.test(table(dst, mut))$conf.int[2]
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#-------------------
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# chi sq estimates
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#-------------------
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estimate_chisq = chisq.test(table(dst, mut))$statistic; estimate_chisq
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est_chisq = estimate_chisq[[1]]; print(est_chisq)
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pval_chisq = chisq.test(table(dst, mut))$p.value
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# build a row to append to df
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row = data.frame(mutation = m
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, af = afs
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, beta_logistic = beta_logistic
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, or_logistic = or_logistic
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, pval_logistic = pval_logistic
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, se_logistic = se_logistic
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, zval_logistic = zval_logistic
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, ci_low_logistic = ci_lower_logistic
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, ci_hi_logistic = ci_upper_logistic
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, or_mychisq = or_mychisq
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, or_fisher = or_fisher
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, pval_fisher = pval_fisher
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, ci_low_fisher= ci_lower_fisher
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, ci_hi_fisher = ci_upper_fisher
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, est_chisq = est_chisq
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, pval_chisq = pval_chisq
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)
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#print(row)
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ors_df <<- rbind(ors_df, row)
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})
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#%%======================================================
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# Writing file with calculated ORs and AFs
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cat(paste0('writing output file: '
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, '\nFilen: ', outfile_af_or))
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write.csv(ors_df, outfile_af_or
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, row.names = F)
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cat(paste0('Finished writing:'
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, outfile_af_or
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, '\nNo. of rows: ', nrow(ors_df)
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, '\nNo. of cols: ', ncol(ors_df)))
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#%%======================================================
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cat('\n======sneak peek into a few muts with prominent or and p-vals=======\n')
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cat(paste0('======================================='
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, '\nmutation with highest logistic OR:'
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, '\n=======================================\n'))
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print(ors_df[which(ors_df$or_logistic == max(ors_df$or_logistic)),])
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cat(paste0('======================================='
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, '\nmutation with highest mychisq OR:'
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, '\n=======================================\n'))
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print(ors_df[which(ors_df$or_mychisq == max(ors_df$or_mychisq)),])
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# gives too many due to Inf
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#cat(paste0('======================================='
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#, '\nmutation with highest fisher OR:'
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#, '\n=======================================\n'))
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#print(ors_df[which(ors_df$or_fisher == max(ors_df$or_fisher)),])
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cat(paste0('======================================='
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, '\nmutation with lowest logistic pval:'
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, '\n=======================================\n'))
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print(ors_df[which(ors_df$pval_logistic == min(ors_df$pval_logistic)),])
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cat(paste0('======================================='
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, '\nmutation with lowest fisher pval:'
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, '\n=======================================\n'))
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print(ors_df[which(ors_df$pval_fisher == min(ors_df$pval_fisher)),])
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#**********************************************************
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cat('End of script: calculated AF, OR, pvalues and saved file')
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#**********************************************************
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