saving previous stuff from work
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2 changed files with 18 additions and 198 deletions
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getwd()
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getwd()
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setwd("~/git/LSHTM_analysis/mcsm_analysis/pyrazinamide/scripts/plotting")
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setwd("~/git/LSHTM_analysis/mcsm_analysis/pyrazinamide/scripts/plotting")
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getwd()
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getwd()
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source("../combining_two_df.R")
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source("../Header_TT.R")
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getwd()
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setwd("~/git/LSHTM_analysis/mcsm_analysis/pyrazinamide/scripts/plotting")
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getwd()
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source("../Header_TT.R")
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source("../Header_TT.R")
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source("../barplot_colour_function.R")
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source("../combining_two_df.R")
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############################################################
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#<<<<<<<<<<<<<<<<<<<<<<<<<
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# Output dir for plots
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############################################################
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out_dir = "~/git/Data/pyrazinamide/output/plots"
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source("subcols_axis.R")
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table(mut_pos_cols$lab_bg)
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#blue cornflowerblue green purple white yellow
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#2 2 2 4 117 3
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sum( table(mut_pos_cols$lab_bg) ) == nrow(mut_pos_cols) # should be True
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table(mut_pos_cols$lab_bg2)
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#green white
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#2 128
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sum( table(mut_pos_cols$lab_bg2) ) == nrow(mut_pos_cols) # should be True
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table(mut_pos_cols$lab_fg)
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#black white
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#124 6
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sum( table(mut_pos_cols$lab_fg) ) == nrow(mut_pos_cols) # should be True
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# very important!
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my_axis_colours = mut_pos_cols$lab_fg
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# now clear mut_pos_cols
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rm(mut_pos_cols)
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###########################
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# 2: Plot: DUET scores
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###########################
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#==========================
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# Plot 2: Barplot with scores (unordered)
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# corresponds to DUET_outcome
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# Stacked Barplot with colours: DUET_outcome @ position coloured by
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# stability scores. This is a barplot where each bar corresponds
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# to a SNP and is coloured by its corresponding DUET stability value.
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# Normalised values (range between -1 and 1 ) to aid visualisation
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# NOTE: since barplot plots discrete values, colour = score, so number of
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# colours will be equal to the no. of unique normalised scores
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# rather than a continuous scale
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# will require generating the colour scale separately.
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#============================
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# sanity checks
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upos = unique(my_df$Position)
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str(my_df$DUET_outcome)
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colnames(my_df)
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#===========================
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# Data preparation for plots
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#===========================
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#!!!!!!!!!!!!!!!!!
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# REASSIGNMENT
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# REASSIGNMENT
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df <- my_df
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my_df = merged_df3_comp
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#!!!!!!!!!!!!!!!!!
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# delete variables not required
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rm(merged_df2, merged_df2_comp, merged_df3, merged_df3_comp)
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# quick checks
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colnames(my_df)
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str(my_df)
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#<<<<<<<<<<<<<<<<<<<<<<<<
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# REASSIGNMENT
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df = my_df
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rm(my_df)
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rm(my_df)
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# sanity checks
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# should be a factor
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is.factor(df$DUET_outcome)
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#TRUE
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table(df$DUET_outcome)
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table(df$DUET_outcome)
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#Destabilizing Stabilizing
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#288 47
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# should be -1 and 1
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min(df$ratioDUET)
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max(df$ratioDUET)
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# sanity checks
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# very important!!!!
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tapply(df$ratioDUET, df$DUET_outcome, min)
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#Destabilizing Stabilizing
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#-1.0000000 0.01065719
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tapply(df$ratioDUET, df$DUET_outcome, max)
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#Destabilizing Stabilizing
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#-0.003875969 1.0000000
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# check unique values in normalised data
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u = unique(df$ratioDUET) # 323
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# %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
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# Run this section if rounding is to be used
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# specify number for rounding
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n = 3
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df$ratioDUETR = round(df$ratioDUET, n) # 335, 40
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u = unique(df$ratioDUETR) # 287
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# create an extra column called group which contains the "gp name and score"
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# so colours can be generated for each unique values in this column
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my_grp = df$ratioDUETR
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df$group <- paste0(df$DUET_outcome, "_", my_grp, sep = "") # 335,41
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# Call the function to create the palette based on the group defined above
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colours <- ColourPalleteMulti(df, "DUET_outcome", "my_grp")
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my_title = "Protein stability (DUET)"
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library(ggplot2)
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# axis label size
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my_xaxls = 13
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my_yaxls = 15
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# axes text size
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my_xaxts = 15
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my_yaxts = 15
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# no ordering of x-axis according to frequency
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g = ggplot(df, aes(factor(Position, ordered = T)))
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g +
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geom_bar(aes(fill = group), colour = "grey") +
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scale_fill_manual( values = colours
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, guide = 'none') +
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theme( axis.text.x = element_text(size = my_xaxls
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, angle = 90
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, hjust = 1
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, vjust = 0.4)
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, axis.text.y = element_text(size = my_yaxls
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, angle = 0
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, hjust = 1
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, vjust = 0)
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, axis.title.x = element_text(size = my_xaxts)
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, axis.title.y = element_text(size = my_yaxts ) ) +
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labs(title = my_title
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, x = "Position"
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, y = "Frequency")
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class(df$lab_bg)
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# make this a named vector
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# define cartesian coord
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my_xlim = length(unique(df$Position)); my_xlim
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# axis label size
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my_xals = 15
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my_yals = 15
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# axes text size
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my_xats = 15
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my_yats = 18
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# using geom_tile
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g = ggplot(df, aes(factor(Position, ordered = T)))
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g +
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coord_cartesian(xlim = c(1, my_xlim)
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, ylim = c(0, 6)
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, clip = "off") +
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geom_bar(aes(fill = group), colour = "grey") +
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scale_fill_manual( values = colours
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, guide = 'none') +
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geom_tile(aes(,-0.8, width = 0.9, height = 0.85)
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, fill = df$lab_bg) +
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geom_tile(aes(,-1.2, width = 0.9, height = -0.2)
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, fill = df$lab_bg2) +
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# Here it's important to specify that your axis goes from 1 to max number of levels
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theme( axis.text.x = element_text(size = my_xats
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, angle = 90
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, hjust = 1
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, vjust = 0.4
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, colour = my_axis_colours)
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, axis.text.y = element_text(size = my_yats
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, angle = 0
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, hjust = 1
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, vjust = 0)
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, axis.title.x = element_text(size = my_xals)
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, axis.title.y = element_text(size = my_yals )
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, axis.ticks.x = element_blank()
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) +
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labs(title = my_title
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, x = "Position"
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, y = "Frequency")
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class(df$lab_bg)
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# make this a named vector
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# define cartesian coord
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my_xlim = length(unique(df$Position)); my_xlim
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# axis label size
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my_xals = 18
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my_yals = 18
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# axes text size
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my_xats = 14
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my_yats = 18
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my_plot_name = "barplot_PS_acoloured.svg"
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out_file = paste0(out_dir, "/", my_plot_name); outfile
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svg(outfile, width = 26, height = 4)
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svg(out_file, width = 26, height = 4)
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# using geom_tile
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g = ggplot(df, aes(factor(Position, ordered = T)))
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outFile = g +
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coord_cartesian(xlim = c(1, my_xlim)
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, ylim = c(0, 6)
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, clip = "off"
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) +
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geom_bar(aes(fill = group), colour = "grey") +
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scale_fill_manual( values = colours
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, guide = 'none') +
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# geom_tile(aes(,-0.6, width = 0.9, height = 0.7)
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# , fill = df$lab_bg) +
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# geom_tile(aes(,-1, width = 0.9, height = 0.3)
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# , fill = df$lab_bg2) +
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geom_tile(aes(,-0.8, width = 0.9, height = 0.85)
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, fill = df$lab_bg) +
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geom_tile(aes(,-1.2, width = 0.9, height = -0.2)
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, fill = df$lab_bg2) +
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# Here it's important to specify that your axis goes from 1 to max number of levels
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theme( axis.text.x = element_text(size = my_xats
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, angle = 90
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, hjust = 1
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, vjust = 0.4
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, colour = my_axis_colours)
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, axis.text.y = element_text(size = my_yats
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, angle = 0
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, hjust = 1
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, vjust = 0)
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, axis.title.x = element_text(size = my_xals)
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, axis.title.y = element_text(size = my_yals )
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, axis.ticks.x = element_blank()
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) +
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labs(title = ""
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, x = "Position"
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, y = "Frequency")
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print(outFile)
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dev.off()
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@ -64,10 +64,10 @@ str(my_df)
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# Data for plots
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# Data for plots
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#===================
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#===================
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#<<<<<<<<<<<<<<<<<<<<<<<<
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#!!!!!!!!!!!!!!!!!!!!!!!!
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# REASSIGNMENT
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# REASSIGNMENT
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df = my_df
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df = my_df
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#<<<<<<<<<<<<<<<<<<<<<<<<<
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#!!!!!!!!!!!!!!!!!!!!!!!!
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rm(my_df)
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rm(my_df)
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