diff --git a/mcsm_analysis/pyrazinamide/scripts/KS_test_PS.R b/mcsm_analysis/pyrazinamide/scripts/KS_test_PS.R new file mode 100644 index 0000000..5a827c8 --- /dev/null +++ b/mcsm_analysis/pyrazinamide/scripts/KS_test_PS.R @@ -0,0 +1,157 @@ +getwd() +setwd("~/git/LSHTM_analysis/mcsm_analysis/pyrazinamide/scripts/plotting") +getwd() + +######################################################################## +# Installing and loading required packages # +######################################################################## + +source("../Header_TT.R") +#source("../barplot_colour_function.R") +#require(data.table) + +######################################################################## +# Read file: call script for combining df for PS # +######################################################################## + +source("../combining_two_df.R") + +#---------------------- PAY ATTENTION +# the above changes the working dir +#[1] "git/LSHTM_analysis/mcsm_analysis/pyrazinamide/scripts" +#---------------------- PAY ATTENTION + +#========================== +# This will return: + +# df with NA for pyrazinamide: +# merged_df2 +# merged_df3 + +# df without NA for pyrazinamide: +# merged_df2_comp +# merged_df3_comp +#=========================== + +########################### +# Data for plots +# you need merged_df2 or merged_df2_comp +# since this is one-many relationship +# i.e the same SNP can belong to multiple lineages +# using the _comp dataset means +# we lose some muts and at this level, we should use +# as much info as available, hence use df with NA +########################### + +# uncomment as necessary + +#%%%%%%%%%%%%%%%%%%%%%%%%% +# REASSIGNMENT +my_df = merged_df2 +#my_df = merged_df2_comp +#%%%%%%%%%%%%%%%%%%%%%%%%% + +# delete variables not required +rm(merged_df2, merged_df2_comp, merged_df3, merged_df3_comp) + +# quick checks +colnames(my_df) +str(my_df) + +# Ensure correct data type in columns to plot: need to be factor +is.factor(my_df$lineage) +my_df$lineage = as.factor(my_df$lineage) +is.factor(my_df$lineage) + +table(my_df$mutation_info); str(my_df$mutation_info) + +# subset df with dr muts only +my_df_dr = subset(my_df, mutation_info == "dr_mutations_pyrazinamide") +table(my_df_dr$mutation_info) + +######################################################################## +# end of data extraction and cleaning for plots # +######################################################################## + +#========================== +# Run two times: +# uncomment as necessary +# 1) for all muts +# 2) for dr_muts +#=========================== +#%%%%%%%%%%%%%%%%%%%%%%%% +# REASSIGNMENT + +#================ +# for ALL muts +#================ +#plot_df = my_df + +#================ +# for dr muts ONLY +#================ +plot_df = my_df_dr + +#%%%%%%%%%%%%%%%%%%%%%%%% +#============================ +# Plot: Lineage Distribution +# x = mcsm_values, y = dist +# fill = stability +#============================ + +table(plot_df$lineage); str(plot_df$lineage) + +# subset only lineages1-4 +sel_lineages = c("lineage1" + , "lineage2" + , "lineage3" + , "lineage4") + +# uncomment as necessary +df_lin = subset(plot_df, subset = lineage %in% sel_lineages ) + +# refactor +df_lin$lineage = factor(df_lin$lineage) + +table(df_lin$lineage) #{RESULT: No of samples within lineage} +#lineage1 lineage2 lineage3 lineage4 + +length(unique(df_lin$Mutationinformation)) +#{Result: No. of unique mutations the 4 lineages contribute to} + +# sanity checks +r1 = 2:5 # when merged_df2 used: because there is missing lineages +if(sum(table(plot_df$lineage)[r1]) == nrow(df_lin)) { + print ("sanity check passed: numbers match") +} else{ + print("Error!: check your numbers") +} + +#%%%%%%%%%%%%%%%%%%%%%%%%%% +# REASSIGNMENT +df <- df_lin +#%%%%%%%%%%%%%%%%%%%%%%%%%% + +rm(df_lin) + +# COMPARING DISTRIBUTIONS +head(df$lineage) +df$lineage = as.character(df$lineage) + +lin1 = df[df$lineage == "lineage1",]$ratioDUET +lin2 = df[df$lineage == "lineage2",]$ratioDUET +lin3 = df[df$lineage == "lineage3",]$ratioDUET +lin4 = df[df$lineage == "lineage4",]$ratioDUET + +# ks test +ks.test(lin1,lin2) +ks.test(lin1,lin3) +ks.test(lin1,lin4) + +ks.test(lin2,lin3) +ks.test(lin2,lin4) + +ks.test(lin3,lin4) + + +