consurf tile fixes
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0234a8f77b
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2 changed files with 169 additions and 163 deletions
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@ -12,81 +12,79 @@
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# wideP():
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# input args
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#==========================================================
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wideP_consurf <- function(plot_df
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, xvar_colname = "position"
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, yvar_colname = "consurf_score"
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, yvar_colourN_colname = "consurf_colour_rev" # num from 0-1
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, plot_error_bars = T
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, upper_EB_colname = "consurf_ci_upper"
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, lower_EB_colname = "consurf_ci_lower"
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, plot_type = "point" # default is point
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, point_colours
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, p_size = 2
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, leg_title1 = ""
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, leg_labels = c("0": "Insufficient Data"
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, "1": "Variable"
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, "2", "3", "4", "5", "6", "7", "8"
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, "9": "Conserved")
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, panel_col = "white"
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, panel_col_fill = "white"
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# axes title and label sizes
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, x_axls = 12 # x-axis label size
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, y_axls = 15 # y-axis label size
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, x_axts = 12 # x-axis text size
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, y_axts = 12 # y-axis text size
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, default_xtc = "black" # x-axis text colour
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, ptitle = ""
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, xlab = ""
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, ylab = ""
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, pts = 20
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# plot margins
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, t_margin = 0.5
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, r_margin = 0.5
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, b_margin = 1
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, l_margin = 1
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, unit_margin = "cm"
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# Custom 1: x-axis: text colour
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, xtext_colour_aa = F
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, xtext_colour_aa1 = active_aa_pos
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, xtext_colour_aa2 = aa_pos_drug
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, xtext_colours = c("purple", "brown", "black")
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# Custom 2: x-axis: geom tiles ~ lig distance
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, A_xvar_lig = T
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, leg_title2 = "Ligand Distance"
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, lig_dist_colname = LigDist_colname # from globals
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, lig_dist_colours = c("green", "yellow", "orange", "red")
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, tpos0 = 0 # 0 is a magic number that does my sensible default
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, tW0 = 1
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, tH0 = 0.3
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# Custom 3: x-axis: geom tiles ~ active sites and ligand
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, A_xvar_aa = F
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, aa_pos_drug = NULL
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, drug_aa_colour = "purple"
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, tW = 1
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, tH = 0.2
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, active_aa_pos = NULL
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, active_aa_colour = "brown"
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, aa_pos_lig1 = NULL
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, aa_colour_lig1 = "blue"
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, tpos1 = 0
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, aa_pos_lig2 = NULL
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, aa_colour_lig2 = "cyan"
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, tpos2 = 0
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, aa_pos_lig3 = NULL
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, aa_colour_lig3 = "cornflowerblue"
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, tpos3 = 0
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, default_gt_clr = "white"
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, debug=FALSE
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wideP_consurf2 <- function(plot_df
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, xvar_colname = "position"
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, yvar_colname = "consurf_score"
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, yvar_colourN_colname = "consurf_colour_rev" # num from 0-1
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, plot_error_bars = T
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, upper_EB_colname = "consurf_ci_upper"
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, lower_EB_colname = "consurf_ci_lower"
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, plot_type = "point" # default is point
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, point_colours
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, p_size = 2
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, leg_title1 = ""
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, leg_labels = c("0": "Insufficient Data"
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, "1": "Variable"
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, "2", "3", "4", "5", "6", "7", "8"
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, "9": "Conserved")
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, panel_col = "white"
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, panel_col_fill = "white"
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# axes title and label sizes
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, x_axls = 12 # x-axis label size
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, y_axls = 15 # y-axis label size
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, x_axts = 12 # x-axis text size
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, y_axts = 12 # y-axis text size
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, default_xtc = "black" # x-axis text colour
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, ptitle = ""
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, xlab = ""
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, ylab = ""
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, pts = 20
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# plot margins
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, t_margin = 0.5
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, r_margin = 0.5
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, b_margin = 1
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, l_margin = 1
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, unit_margin = "cm"
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# Custom 1: x-axis: text colour
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, xtext_colour_aa = F
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, xtext_colour_aa1 = active_aa_pos
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, xtext_colour_aa2 = aa_pos_drug
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, xtext_colours = c("purple", "brown", "black")
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# Custom 2: x-axis: geom tiles ~ lig distance
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, annotate_ligand_distance = T
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, leg_title2 = "Ligand Distance"
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, lig_dist_colname = LigDist_colname # from globals
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, lig_dist_colours = c("green", "yellow", "orange", "red")
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, tpos0 = 0 # 0 is a magic number that does my sensible default
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, tW0 = 1
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, tH0 = 0.3
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# Custom 3: x-axis: geom tiles ~ active sites and ligand
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, annotate_active_sites = F
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, aa_pos_drug = aa_pos_drug
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, drug_aa_colour = "purple"
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, active_aa_pos = NULL
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, active_aa_colour = "brown"
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, aa_pos_lig1 = NULL
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, aa_colour_lig1 = "blue"
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, tpos1 = 0
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, aa_pos_lig2 = NULL
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, aa_colour_lig2 = "cyan"
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, tpos2 = 0
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, aa_pos_lig3 = NULL
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, aa_colour_lig3 = "cornflowerblue"
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, tpos3 = 0
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, default_gt_clr = "white"
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, debug=FALSE
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){
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if(missing(point_colours)){
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@ -119,7 +117,7 @@ wideP_consurf <- function(plot_df
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# Build data with colours
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# ~ ligand distance
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#=========================
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if (A_xvar_lig){
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if (annotate_ligand_distance){
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cat("\nAnnotating x-axis ~", lig_dist_colname, "requested...")
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#-------------------------------------
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@ -159,7 +157,7 @@ wideP_consurf <- function(plot_df
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ligDcolKey <- data.frame(ligD_colours = lig_cols
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, lig_distR = ligD_valsR); ligDcolKey
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names(ligDcolKey)
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#names(ligDcolKey)
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cat("\nSuccessful: Mapping b/w", lig_dist_colname, "and colours")
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#-------------------------------------
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@ -168,11 +166,9 @@ wideP_consurf <- function(plot_df
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plot_df = merge(plot_df, ligDcolKey, by = 'lig_distR')
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plot_df_check = as.data.frame(cbind(position = plot_df[[xvar_colname]]
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, ligD = plot_df[[lig_dist_colname]]
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, ligDR = plot_df$lig_distR
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, ligD_cols = plot_df$ligD_colours))
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} else{
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plot_df = plot_df
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, ligD = plot_df[[lig_dist_colname]]
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, ligDR = plot_df$lig_distR
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, ligD_cols = plot_df$ligD_colours))
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}
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###############################################
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@ -184,7 +180,7 @@ wideP_consurf <- function(plot_df
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# ~ on active sites
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#==========================
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if(A_xvar_aa) {
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if(annotate_active_sites) {
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cat("\nAnnotation for xvar requested. Building colours for annotation...")
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aa_colour_colname = "bg_all"
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@ -196,9 +192,9 @@ wideP_consurf <- function(plot_df
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# column colour 0: Active site + drug binding sites
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#--------------------------------------------------
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plot_df[[aa_colour_colname]] = ifelse(plot_df[[xvar_colname]]%in%aa_pos_drug
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, drug_aa_colour
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, ifelse(plot_df[[xvar_colname]]%in%active_aa_pos
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, active_aa_colour, default_gt_clr ))
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, drug_aa_colour
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, ifelse(plot_df[[xvar_colname]]%in%active_aa_pos
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, active_aa_colour, default_gt_clr ))
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plot_df[[aa_colour_colname]]
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cat("\nColumn created 'bg_all':", length(plot_df[[aa_colour_colname]]))
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@ -208,20 +204,20 @@ wideP_consurf <- function(plot_df
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cat("\nAssigning colours to drug binding and ligand-1 binding residues")
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plot_df[[aa_colour_colname1]] = plot_df[[aa_colour_colname]]
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plot_df[[aa_colour_colname1]] = ifelse(plot_df[[xvar_colname]]%in%aa_pos_lig1
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, aa_colour_lig1, plot_df[[aa_colour_colname]])
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, aa_colour_lig1, plot_df[[aa_colour_colname]])
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#------------------------------------------------
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# column colour 2: Ligand 2
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#------------------------------------------------
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plot_df[[aa_colour_colname2]] = plot_df[[aa_colour_colname1]]
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plot_df[[aa_colour_colname2]] = ifelse(plot_df[[xvar_colname]]%in%aa_pos_lig2
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, aa_colour_lig2, plot_df[[aa_colour_colname1]])
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, aa_colour_lig2, plot_df[[aa_colour_colname1]])
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#------------------------------------------------
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# column colour 3: Ligand 3
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#------------------------------------------------
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plot_df[[aa_colour_colname3]] = plot_df[[aa_colour_colname2]]
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plot_df[[aa_colour_colname3]] = ifelse(plot_df[[xvar_colname]]%in%aa_pos_lig3
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, aa_colour_lig3, plot_df[[aa_colour_colname2]])
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, aa_colour_lig3, plot_df[[aa_colour_colname2]])
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}
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###################
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@ -237,12 +233,13 @@ wideP_consurf <- function(plot_df
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ymax = max(plot_df[[yvar_colname]]); ymax
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g = ggplot(plot_df, aes_string(x = sprintf("factor(%s)", xvar_colname)
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, y = yvar_colname
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, colour = sprintf("factor(%s)", yvar_colourN_colname)
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, y = yvar_colname
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, colour = sprintf("factor(%s)", yvar_colourN_colname)
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))
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#"if SPECIAL do SPECIAL THING, otherwise do NORMAL THING"
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if (plot_type == "bar"){
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cat('\ndoing bar plot')
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g0 = g +
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geom_bar(stat = "identity")
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}
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@ -256,6 +253,7 @@ wideP_consurf <- function(plot_df
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}
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if (plot_error_bars){
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cat('\nplotting error bars')
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g0 = g0 +
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geom_errorbar(aes(ymin = eval(parse(text = lower_EB_colname))
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, ymax = eval(parse(text = upper_EB_colname))
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@ -291,7 +289,7 @@ wideP_consurf <- function(plot_df
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, b = b_margin
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, l = l_margin
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, unit = unit_margin))+
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guides(colour = guide_legend(title = "ConsurfXXXX")) +
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#guides(colour = guide_legend(title = "ConsurfXXXX")) +
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labs(title = ptitle
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, x = xlab
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@ -302,7 +300,7 @@ wideP_consurf <- function(plot_df
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#------------------
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# yayy
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g1_leg = ggplot(plot_df, aes_string(x = sprintf("factor(%s)"
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, xvar_colname) ))
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, xvar_colname) ))
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g1_leg = g1_leg + geom_bar(); g1_leg
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g1_leg = g1_leg + geom_bar(aes_string(fill = sprintf("factor(%s)"
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, yvar_colourN_colname)))
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@ -320,7 +318,7 @@ wideP_consurf <- function(plot_df
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#-------
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# plot
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#-------
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if(A_xvar_lig){ # 0 is a magic number that does my sensible default
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if(annotate_ligand_distance){ # 0 is a magic number that does my sensible default
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if (tpos0 == 0){
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tpos0 = ymin-0.5
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}
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@ -339,8 +337,6 @@ wideP_consurf <- function(plot_df
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, "\nNo. of colours:", n_colours)
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g2 = g1 + geom_tile(aes(, tpos0
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# g2 = g1 + geom_tile(aes(, tpos0
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, width = tW0
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, height = tH0)
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, fill = plot_df$ligD_colours
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@ -349,14 +345,19 @@ wideP_consurf <- function(plot_df
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#cat("Nrows of plot df", length(plot_df$ligD_colours))
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out = g2
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cat('\nOut is g2')
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}else{
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cat('\nOut is g1')
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out = g1
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}
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#==============================================
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# x-axis: geom_tiles ~ active sites and others
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#==============================================
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if(A_xvar_aa){
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if(annotate_active_sites){
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cat('\nDrug Positions: ',aa_pos_drug)
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cat ('\nLig 1 Positions:', aa_pos_lig1)
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cat ('\nLig 2 Positions:', aa_pos_lig2)
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cat ('\nLig 2 Positions:', aa_pos_lig3)
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#tpos = 0
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#tW = 1
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#tH = 0.2
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@ -366,67 +367,70 @@ wideP_consurf <- function(plot_df
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#---------------------
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if (all(!is.null(active_aa_pos) &&
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!is.null(aa_pos_drug) &&
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!is.null(aa_pos_lig1) && !is.null(aa_pos_lig2) && !is.null(aa_pos_lig3))) {
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if (debug){
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cat("\n\nAnnotating xvar with active, drug binding, and Lig 1&2&3 sites")
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cat("\nCreating column colours, column name:", aa_colour_colname3)
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cat("\nDoing Plot with 3 ligands")
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}
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#out = out + geom_tile(aes(,tpos3
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out = out + geom_tile(aes(,2
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, width = tW
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, height = tH )
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!is.null(aa_pos_lig1) &&
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!is.null(aa_pos_lig2) &&
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!is.null(aa_pos_lig3))) {
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#if (debug){
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cat("\n\nAnnotating xvar with active, drug binding, and Lig 1 & 2 & 3 sites")
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#cat("\nCreating column colours, column name:", aa_colour_colname3)
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cat("\nDoing Plot with 3 ligands")
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#}
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out = out + geom_tile(aes(,tpos3
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, width = tW0
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, height = tH0 )
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, fill = plot_df[[aa_colour_colname3]]
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, colour = plot_df[[aa_colour_colname3]]
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, linetype = "solid") +
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geom_tile(aes(, tpos2
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, width = tW
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, height = tH )
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, width = tW0
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, height = tH0 )
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, fill = plot_df[[aa_colour_colname2]]
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, colour = plot_df[[aa_colour_colname2]]
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, linetype = "solid")+
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geom_tile(aes(, tpos1
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, width = tW
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, height = tH)
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, width = tW0
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, height = tH0)
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, fill = plot_df[[aa_colour_colname1]]
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, colour = plot_df[[aa_colour_colname1]]
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, linetype = "solid")
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if (debug){
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cat("\nDone Plot with 3 ligands")
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}
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}
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#if (debug){
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cat("\nDone Plot with 3 ligands")
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#}
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} else {cat("\n3 Lig Plot condition FAIL")}
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#---------------------
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# Add2plot: 2 ligands
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#---------------------
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if (all(!is.null(active_aa_pos) &&
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!is.null(aa_pos_drug) &&
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!is.null(aa_pos_lig1) && !is.null(aa_pos_lig2) && is.null(aa_pos_lig3))) {
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if (debug){
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cat("\n\nAnnotating xvar with active, drug binding, and Lig 1&2 sites")
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cat("\nCreating column colours, column name:", aa_colour_colname2)
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cat("\nDoing Plot with 2 ligands")
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}
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!is.null(aa_pos_lig1) &&
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!is.null(aa_pos_lig2) &&
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is.null(aa_pos_lig3))) {
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#if (debug){
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cat("\n\nAnnotating xvar with active, drug binding, and Lig 1&2 sites")
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cat("\nCreating column colours, column name:", aa_colour_colname2)
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cat("\nDoing Plot with 2 ligands")
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#}
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out = out +
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geom_tile(aes(, tpos2
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, width = tW
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, height = tH)
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, width = tW0
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, height = tH0)
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, fill = plot_df[[aa_colour_colname2]]
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, colour = plot_df[[aa_colour_colname2]]
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, linetype = "solid")+
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geom_tile(aes(, tpos1
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, width = tW
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, height = tH)
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, width = tW0
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, height = tH0)
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, fill = plot_df[[aa_colour_colname1]]
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, colour = plot_df[[aa_colour_colname1]]
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, linetype = "solid")
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if (debug){
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cat("\nDone Plot with 2 ligands")
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}
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}
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#if (debug){
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cat("\nDone Plot with 2 ligands")
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#}
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} else {cat("\n2 Lig Plot condition FAIL")}
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#---------------------
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# Add2plot: 1 ligand
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@ -434,53 +438,54 @@ wideP_consurf <- function(plot_df
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if (all(!is.null(active_aa_pos) &&
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!is.null(aa_pos_drug) &&
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!is.null(aa_pos_lig1) && is.null(aa_pos_lig2) && is.null(aa_pos_lig3))) {
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if (debug){
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cat("\n\nAnnotating xvar with active, drug binding, and Lig 1 sites")
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cat("\nCreating column colours, column name:", aa_colour_colname1)
|
||||
|
||||
cat("\nDoing Plot with 1 ligands")
|
||||
}
|
||||
#if (debug){
|
||||
cat("\n\nAnnotating xvar with active, drug binding, and Lig 1 sites")
|
||||
cat("\nCreating column colours, column name:", aa_colour_colname1)
|
||||
|
||||
cat("\nDoing Plot with 1 ligands")
|
||||
#}
|
||||
out = out +
|
||||
geom_tile(aes(, tpos1
|
||||
, width = tW
|
||||
, height = tH)
|
||||
, width = tW0
|
||||
, height = tH0)
|
||||
, fill = plot_df[[aa_colour_colname1]]
|
||||
, colour = plot_df[[aa_colour_colname1]]
|
||||
, linetype = "solid")
|
||||
|
||||
cat("\nDone Plot with 1 ligand")
|
||||
|
||||
}
|
||||
} else {cat("\n3 Lig Plot condition FAIL")}
|
||||
|
||||
#-----------------------------------
|
||||
# Add2plot:NO ligands
|
||||
# No Ligs: Just drug and active site
|
||||
# DEFAULT: A_xvar_aa == TRUE
|
||||
# DEFAULT: annotate_active_sites == TRUE
|
||||
#----------------------------------
|
||||
if (all(!is.null(active_aa_pos) &&
|
||||
!is.null(aa_pos_drug) &&
|
||||
is.null(aa_pos_lig1) &&
|
||||
is.null(aa_pos_lig2) &&
|
||||
is.null(aa_pos_lig3))) {
|
||||
if (debug){
|
||||
cat("\n\nAnnotating xvar with active and drug binding sites")
|
||||
cat("\nCreating column colours, column name:", aa_colour_colname)
|
||||
cat("\nDoing Plot with 0 ligands: active and drug site only")
|
||||
}
|
||||
#if (debug){
|
||||
cat("\n\nAnnotating xvar with active and drug binding sites")
|
||||
cat("\nCreating column colours, column name:", aa_colour_colname)
|
||||
cat("\nDoing Plot with 0 ligands: active and drug site only")
|
||||
#}
|
||||
out = out + geom_tile(aes(, tpos3
|
||||
, width = tW
|
||||
, height = tH)
|
||||
, width = tW0
|
||||
, height = tH0)
|
||||
, fill = plot_df[[aa_colour_colname]]
|
||||
, colour = plot_df[[aa_colour_colname]]
|
||||
, linetype = "solid")
|
||||
if (debug){
|
||||
cat("\nDone Plot with: Active and Drug sites")
|
||||
}
|
||||
#if (debug){
|
||||
cat("\nDone Plot with: Active and Drug sites")
|
||||
}
|
||||
}else{
|
||||
#}
|
||||
} else {
|
||||
cat("\nNo annotation for additional ligands on xvar requested")
|
||||
}
|
||||
if (A_xvar_lig){
|
||||
if (annotate_ligand_distance){
|
||||
cat('\nOutput: Plot + distance heat-bar + other stuff')
|
||||
legs = cowplot::plot_grid(legend1
|
||||
, generate_distance_legend(plot_df, yvar_colname = yvar_colname)
|
||||
, ncol = 1
|
||||
|
|
@ -505,13 +510,13 @@ wideP_consurf <- function(plot_df
|
|||
#return(out2)
|
||||
|
||||
}
|
||||
|
||||
|
||||
# wideP_consurf(
|
||||
# merged_df3,
|
||||
# A_xvar_aa = T,
|
||||
# active_aa_pos = active_aa_pos,
|
||||
# aa_pos_lig1 = aa_pos_lig1,
|
||||
# aa_pos_lig2 = aa_pos_lig2,
|
||||
# aa_pos_lig3 = aa_pos_lig3,
|
||||
#
|
||||
# wideP_consurf2(
|
||||
# merged_df3,
|
||||
# annotate_active_sites = TRUE,
|
||||
# active_aa_pos = active_aa_pos,
|
||||
# aa_pos_drug = aa_pos_drug,
|
||||
# aa_pos_lig1 = aa_pos_lig1,
|
||||
# aa_pos_lig2 = aa_pos_lig2,
|
||||
# aa_pos_lig3 = aa_pos_lig3,
|
||||
# debug=T)
|
||||
Loading…
Add table
Add a link
Reference in a new issue